유전자재조방법으로 제조한 γ-interferon의 항바이러스 작용측정

남상윤,신영오,남정구 대한바이러스학회 1989 Journal of Bacteriology and Virology Vol.19 No.2

열상 처치 마우스에서 백혈구 분포의 변화

남상윤 전주대학교 자연과학종합연구소 1997 전주대학교 자연과학연구소 학술논문집 Vol.10 No.1

It has been well documented that global suppression of the immune responses occurs after thermal injury and these changes resulted in decreased resistance to infection, which is a leading cause of high mortality of burn patients. Regarding to numerical changes of lymphocytes related to this immune defects, a lot of controversial results have been reported. In this study, the distribution of leukocytes was investigated in thermally injured mice. Mice (Balb/c) were anesthetized by intraperitoneal injection of 2,2,2-tribromoethanol and thermally injured by immersion of hair-removed dorsal skin (15% total body surface) in a boiling water bath (ca. 96℃). Mortality of mice injured for 7, 9 and 11 seconds was <5%, 25 and 100%, respectively. Almost all of mice injured for 7 seconds recovered from wounds after 40 days postinjury, which were used for all experiments. Significant decrease of peripheral blood leukocyte (PBL), lymph node cell (I,NC) and spleen cell (SPC), thymocyte(Thy) numbers was observed at day 2 and followed by gradual increase to (PBL) or over normal level (LNC and SPC) until day 24 of injury, whereas thymocytes sustained lowered numbers. Such increase of cell number coincided with higher proportion of PMN, particularly in spleen (5% vs 30%). Reduction of lymphocyte proportion was due to relative increase of PMN number, not the decrease of absolute number of lymphocytes. This increasing number of PMN may explain the diminished immune response, assessed with whole spleen cells in a murine model. These results also suggest that trafficking of PMN may be important for the recovery from thermal trauma. In addition, the Th/Ts ratio of injured mice was comparable to that of control group, which is not likely to be a critical factor leading to alteration of immunity in thermal injury.

마우스 정소에 있어서 Phospholipid Hydroperoxide Glutathione Peroxidase 유전자의 분리 및 발현양상

남상윤 한국실험동물학회 1999 한국실험동물학회 학술발표대회 논문집 Vol.1999 No.-

Exposure to genistein does not adversely affect the reproductive system in adult male mice adapted to a soy-based commercial diet

남상윤,이범준,강종구,정은용,윤영원,백인정,윤정민,이윤복,손현수,이재용,김강성 대한수의학회 2004 Journal of Veterinary Science Vol.5 No.3

Genistein, a soybean-originated isoflavone, is widely consumed by humans for putative beneficial health effects but its estrogenic activity may affect adversely the development of male reproductive system. Five-week-oldICR mice were purchased and fed with a soybean-based Purina Chow diet until 6 months of age. The animals were xposed by gavage to genistein (2.5 mg/kg/day) or 17β-estradiol (7.5 μg/kg/day) for five weeks. Corn oil was used for the negative control. The animals were fed the caseinbasedAIN-76A diet throughout the experimental periods. There were no significant differences in body and organ weights of mice among experimental groups. Nosignificant differences in sperm counts and sperm motile characteristics were found between the control and the genistein groups. Treatment of 17β-estradiol caused asignificant decrease in epididymal sperm counts compared to the control (p < 0.05). The level of phospholipid hydroxide glutathione peroxidase in the epididymis ofmice exposed to genistein was significantly higher than that of the control mice (p < 0.05). 17β-estradiol treatment caused a reduction of germ cells in the testis andhyperplasia of mucosal fold region in the prostate of mice. Genistein treatment did not cause any lesion in the testis,epididymis, and prostate. These results suggest thatdietary uptake of genistein at adult stage of life may not affect male reproductive system and functions.

身體의 自由에 關한 小考

南相倫 成均館大學校 法政大學 法律學會 1964 成均法學 Vol.9 No.1

전기기사 법정교육을 마치고

남상윤 한국전기기술인협회 1993 電力技術人 Vol.133 No.-

마우스 B 림프구에서 비특이적 자극에 의한 CD30 발현의 유도

남상윤 전주대학교 자연과학종합연구소 1997 전주대학교 자연과학연구소 학술논문집 Vol.10 No.3

CD30 was originally identified on Hodgkin and Reed-Sternberg (H-RS) cells and subsequently defined as an activation antigen. CD30 expression is observed on activated, but not on resting, T and B lymphocytes. Despite of numericcal studies, the functions of CD30 in physiological condition remains open question. Moreover, CD30 expression of normal B lymphocytes has been poorly documented. In this study, CD30 expression of murine B lymphocytes and its regulation was analyzed. B cell-enriched (90%) spleen (SP-B) cells obtained by nylon-wool adherence were used for activation with LPS or plate-bound anti-mouse IgM. LPS stimulation induced minimal expression of CD30 and addition of cytokines (IL 2 or IL 4) resulted in higher number of activated cells, but not higher expression of CD30. Substantial CD30 expression of SP-B cells was induced by stimulation with plate-bound anti-mouse IgM in a dose dependent manner. IL 4 upregulated proliferation of and CD30 expression on activated SP-B cells. IL 2 enhancd B cell proliferation but not CD30 expression and also did not show any additional effect on IL 4-mediated upregulation of CD30 expression. These data suggest that the signaling pathway for B cell proliferation is different from that for induction of CD30 expression and T and B cells may show distinct response to cytokines in CD30 expression.

Concurrent Impairment of Th1 and Th2 Response in Thermal Injury

남상윤,도정수,김영현 대한미생물학회 2002 Journal of Bacteriology and Virology Vol.32 No.4

가정에서의 전기안전 대책

남상윤 대한전기협회 2000 전기저널 Vol. No.

생활수준의 향상으로 일반가정에서 사용하는 전기용품의 종류도 무척 다양해졌으며 사용량도 급격히 증가되어 이로 인한 전기재해(화재, 감전)가 끊이지 않고 있다. 행정자치부 화재통계 연보에 따르면 ''99년도 총 화재발생건수 33,856건 중 전기로 인한 화재가 11,204건으로 33.1$\%$의 높은 점유율을 보이고 있어 일반가정에서의 전기안전 생활화가 절실히 요구되고 있는 실정이다. 일반 가정생활에서 편리하게 사용하고 있는 전기를 보다 더 안전하게 사용하기 위해서 우리 모두가 꼭 알아두어야 할 전기설비에 관한 기초상식, 전기사고의 종류와 예방대책, 정전 시 대처요령 등을 알아본다.

랫드의 화상모델에서 전복껍질과 참기름의 치료효과

남상윤,이범준 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.2

This study was performed to investigate effect of a mixture of ear shell ash and sesame oil on bum in a Sprague-Dawley rat model. Sixty male rats were assigned to 3 experimental groups including negative and positive control groups, and a test material group. The test compound was applied to formally once a day f3r 7 days. Distilled water was applied as a relative positive control. After dermal applications f3r 3, 5, and 7 days, blood hematology and skin histopathological examination were performed. Inorganic compounds in ear shell ash was mainlycalcium oxide(92%). The body weights of positive control rats were significantly low at day 3, 5, and 7 as compared with negative control and test material groups. There was no significant difference in body weight between negative control and test material groups throughout the experimental period. WBC counts in both positive control and test material groups were higher than those in negative control group, indicating progress of inflammatory reaction. In differential leucocyte count, panulocytes including neutrophils and eosinophils were higher in positive control and test material groups than negative control group. Hemoglobin and hematocrit values were lower in homing model rats, probably resulting from hemorrhage in dermis. Skin histopathology showed severe full thickness coagulative necrosis in epidermis and dermis, hemorrhage and infiltration of leucocytes in dermis in both positive control and test material groups throughout the experimental period. These results indicate that the test materials, ear shell ash plus sesame oil, moderately ameliorate homing probably concerning to process of skin injury repair including inflammation and fibrosis