Saccharomyces cerevisiae에서 Cycloinulooligosaccharide Fructanotransferase 유전자의 표층 발현

김현진,이재형,김현철,김연희,권현주,남수완,Kim, Hyun-Jin,Lee, Jae-Hyung,Kim, Hyun-Chul,Kim, Yeon-Hee,Kwon, Hyun-Ju,Nam, Soo-Wan 한국생명과학회 2007 생명과학회지 Vol.17 No.2

Paenibacillus macerans 유래의 cycloinulooligosaccharide fructanotransferase (CFTase) 유전자(cft, 2832 bp, 103.8 kDa)를 효모 표층발현 vector인 pCTcon (GAL1 promoter)에 subcloning 하였다. 구축된 pCTECFTN (9.0 kb)를 숙주세포인 s. cerevisiae EBY100에 형질전환한 후, uracil이 결핍된 배지와 inulin 함유배지에서 선별하였다. cft 유전자는 GAL1 promoter에 의해 효모 형질전환체에서 성공적으로 발현되었다. inulin으로부터 cyclofructans (CFs)로 생산하는 효소적 능력으로부터 표층발현 유무를 확인하였다. YPDG배지에서 48시간 배양 후 분획한 균체는 5.52 unit/ l 의 활성을 보였다. CF 생산을 위한 효소의 최적 반응 조건으로 pH 8.0, 반응온도 $50^{\circ}C$, 기질농도 5%, 기질은 Jerusalem artichoke 등의 inulin과 표층 발현 CFTase 효소반응 결과, cycloinulohexaose (CF6), cycloinuloheptaose (CF7), 그리고 cycloinulooctaose (CF8)이 생성되었고, 이 중에서 CF6가 주 생성물이었다. The cycloinulooligosaccharide fructanotransferase (CFTase) gene (cft) from Paenibacillus macerans was subcloned into the surface display vector, pCTcon (GAL1 promoter). The constructed plasmid, pCTECFTN (9.0 kb) was introduced to S. cerevisiae EBY100 cell and then east transformants were selected on the synthetic defined medium lacking uracil and on the inulin containing medium. The surface display of CFTase was confirmed by immunofluorescence microscopy and its enzymatic ability to form cycloinulooligosaccharides(cyclofructans, CFs) from inulin. The total activity of the CFTase was reached about 5.52 unit/1 by cultivation of yeast transformant on YPDG medium. The optimized conditions determined were as follows; pH, 8.0; temperature, $50^{\circ}C$ ; substrate concentration, 5%; inulin source, Jerusalem artichoke. By the reaction with inulin, CFs consisting of cycloinulohexaose (CF6), cycloinuloheptaose (CF7), and cycloinulooctaose (CF8) were produced and CF6 was the major product.

Burden of Disease Due to Outdoor Air Pollution in Korea: Based on PM₁₀

김현진,윤석준,김형수,이건세,김은정,조민우,오인환,Kim, Hyun-Jin,Yoon, Seok-Jun,Kim, Hyeong-Su,Lee, Kun-Sei,Kim, Eun-Jung,Jo, Min-Woo,Oh, In-Hwan Korean Society of Environmental Health 2011 한국환경보건학회지 Vol.37 No.5

Purpose: This study measured the burden of disease in Korea related to outdoor air pollution using disability-adjusted life year (DALY). Materials and Methods: As a risk factor of outdoor air pollution, particulate matter with a diameter less than 10 ${\mu}m$($PM_{10}$) was used. First, $PM_{10}$-related diseases and their relative risk (RR) were selected by means of a literature review. Second, population attributable fractions were computed by using formulae including RR and population exposure to $PM_{10}$. Third, DALYs of $PM_{10}$-related diseases in Korea were estimated. Finally, the attributable burden of disease due to $PM_{10}$ was measured as the sum of the products that multiplied the DALYs of $PM_{10}$-related diseases by their population attributable fractions. Results: The disease burden of PM10 was 6.9 DALY per 1,000 persons in 2007. The attributable burden of $PM_{10}$ was 2.68 for lung cancer, 2.41 for COPD, 0.62 for ischemic heart disease, 0.61 for pneumonia, 0.55 for asthma, and 0.03 for preterm. Conclusions: This study showed the environmental burden of disease of $PM_{10}$ and burden of $PM_{10}$-related disease through objective data. It also suggested that active efforts are needed to continuously measure and reduce the burden of environmental diseases in Korea.

감모후유증(感冒後遺症)으로 기허형(氣虛型)호흡기 질환을 호소하는 환자의 한약치료에 관한 임상연구

김현진,한효정,장정아,박은영,안태한,서호석,김진원,Kim, Hyun-Jin,Han, Hyo-Jung,Jang, Jeong-A,Park, Eun-Young,An, Tae-Han,Seo, Ho-Seok,Kim, Jin-Won 대한한의학방제학회 2010 大韓韓醫學方劑學會誌 Vol.18 No.1

Objectives : In order to confirm a remedial effect and related influence of the clinic treatment by prescribing Samchuljojung-tang(蔘朮調中湯), Bojungikqi-tang(補中益氣湯) and Samso-um(蔘蘇飮) to patients who suffering from a chronic cough and rhinorrhea. Methods : 1. In a clinical observation and analysis method, we have selected the 23 patients who have visited National Oriental Medical Center, date from 2007.8.21 to 2008.7.31 and suffering from a chronic cough and rhinorrhea caused by the common cold sequelae. 2. Separated them into three types(I: cough type, II: rhinorrhea type, III: cough & rhinorrhea type). 3. Observed thoroughly the level of symptom and recorded it for each type with questionnaire survey at the beginning set 4. Prescribed Samchuljojung-tang, Bojungikqi-tang and Samso-um for type I, II, III respectively. 5. Observed the level of symptom and treatment afterwards affect, and recorded movements of intensity level for 3 types with same questionnaires. Results : 1. Type I : The survey turned out to be from 9 patients, initial level recorded 31.89 at the entry diagnosis, and next level that means changing of symptoms, recorded to 12.67 after prescribing herb medication. 2. Type II : From 11 patients, initial level was 32.82 and next level was 13.45. 3. Type III : From 3 patients, initial level was 37.67 and next one was 16.00. Conclusions : It is concluded that there is a significant remedial effect and related influence of the clinic treatment for each type of patients who suffering from a chronic cough and rhinorrhea caused by the common cold sequelae.

Ultrastructural Charateristics of a Human Sialolith

김현진,이수근,서봉직,Kim, Hyun-Jin,Lee, Soo-Guen,Suh, Bong-Jik The Korean Academy of Orofacial Pain and Oral Medi 1999 Journal of Oral Medicine and Pain Vol.24 No.4

타석에 관한 연구는 타석증을 보이는 환자에 대한 임상적 특징, 진단 및 치료에서부터 타석의 성분 및 구조 등에 이르기까지 다양한 범위에 걸쳐 이루어지고 있다. 타석의 미세구조에 관한 연구는 타석의 미세구조가 다양한 형태인 것으로 보고되고 있으며, 특히 최근 타석증의 치료에 새롭게 소개되고 있는 체외충격파쇄석술은 타석의 구조에 따라 그 효과가 영향을 받을 수 있을 것으로 사료된다. 이에 저자는 인간 타석의 미세구조에 관한 기본 자료가 필요할 것으로 사료되어 한국인 중년 여성으로부터 적출된 악하선 타석을 광학현미경 및 주사전자현미경을 이용하여 미세구조적 특징을 관찰한 결과, 다음과 같은 결론을 얻었다. 1. 타석은 중심부의 핵, 핵 주변의 층상구조 및 외피막으로 이루어져 있었다. 2. 핵은 비정형의 중심과 상대적으로 균질의 외곽부위로 구성되어 있었다. 3. 핵 주변은 대부분 동심원적인 층상구조를 보였지만 일부분에서는 균질의 구조를 보였다. 4. 타석 단면의 전체직경과 중심부 핵의 직경은 각각 $3,500{\mu}m$와 $1,500{\mu}m$였고, 층상구조를 이루는 각 층의 두께는 위치에 따라 약 $10{\sim}40{\mu}m$ 이내였다.

고령자용 소규모 그룹홈의 생활환경 실태

김현진,안옥희,Kim, Hyun-Jin,An, Ok-Hee 한국주거학회 2008 한국주거학회 논문집 Vol.19 No.3

To investigate the actual conditions on the living environment of group-homes for the elderly and their living environment, 4 group-homes were surveyed. As the results, conditions of location for the group-home of the elderly looked positive, on the other hand, safety for criminal prevention, accessability and convenient facilities were not on level satisfied. The average area of each space in the group-home indicated that the bed room was $11.21\;m^2$, the living room $28.13\;m^2$, the kitchen $23.59\;m^2$, and the bath room had an integrated type of bath room and toilet $7.63\;m^2$. The physical environments of each space in the group-home were investigated, In bed room, door, windows and illumination were relatively good but safety was extremely inferior. Living room mostly looked good except the doorsill which needs refurbishment. In kitchen, cooking table, windows, illumination, ventilator were somewhat satisfactory but gas-warning device should have been installed. In addition, in bath room and toilet, location, door, window, illumination and finished material were satisfactory. But the stepped difference between the bath room and other space, safety-bar, emergency bell and floor heating system were seriously deteriorated and must be improved for the safety of the elderly. Conclusively, living environment of group-home for the elderly is mainly satisfactory but it was necessary to take a consideration for their safety.

Plant Stress Response를 이용한 과채류의 Phytochemicals 함량 증가 기술

김현진,Kim, Hyun-Jin 한국식품연구원 2009 食品技術 Vol.22 No.4

스트레스 완화 목적의 아로마 요법에 관한 문헌고찰

김현진,정수현,정혜인,김경한,Kim, Hyeon-Jin,Jeong, Soo-Hyun,Jeong, Hye-In,Kim, Kyeong Han 대한예방한의학회 2021 대한예방한의학회지 Vol.25 No.2

Objective : This study was aimed to review randomized controlled trials (RCTs) about whether aromatherapy relieves stress. Method : We searched document about criteria to use words like 'Aroma', 'Oil' and 'Stress'. The study included 24 RCTs which were selected by total 167 studies searched in Korean Journal by searching OASIS, ScienceON, KISS, RISS. Cases that cannot be performed alone are excluded. Results : We got 24 domestic standard documents. Of the 24 studies, 14 were for students, and 6 were for patients receiving hospital treatment. Among the 7 treatments, dry-inhalation was used 13 times, and necklace-inhalation was used 9 times. Of the 24 Studies, lavender oil was used 19 times and sweet orange was used 4 times. Among the 28 types of measuring instruments used, 10 related to the autonomic nervous system and 8 STAIs and VASs were used respectively. Conclusion : It was possible to conclude that aromatherapy was effective in relieving stress. Through further research, it is necessary to study effective oil mixing methods, methods for measuring subjective stress, multimodal intervention, and effective intervention periods.

AWS 관측 데이터를 이용한 실시간 웹 디스플레이 및 자료 처리

김현진,정승현,이시우,민경덕,Kim, Hyun-Jin,Jung, Seung-Hyun,Lee, Si-Woo,Min, Kyung-Duck 한국지구과학회 2002 한국지구과학회지 Vol.23 No.7

자동기상관측장비인 AWS(Automatic Weather System)는 교육기관 뿐만아니라 정부 산하 각 지방 도시에 많이 설치되어있다. 그러나 많은 AWS중 실시간으로 웹을 통해 제공되는 기상 정보는 많지 않다. 이에 본 연구에서는 AWS에서 전송되어 오는 데이터를 실시간으로 자동 저장할 수 있도록 하여 실시간으로 디스플레이하고 자료 처리를 하여 교육적으로 활용하고자 하였다. 데이터의 실시간 디스플레이 및 자료 처리를 위해 몇 가지 가능성을 검토했으며 그 가능성을 통해 이를 실현하고자 일차적으로 KNU Weather now, V1.0를 개발하였다. 이 작업의 결과로 지금까지 AWS의 자료를 불러와 사용하기 위해 해오던 불편한 작업이 어느 정도 해소되었고 다시 이를 재가공해 외부에 서비스는 물론, 부가적으로 AWS 관측데이터를 필요로 하는 여러 작업에 이용할 수 있고 또한 데이터 처리 부분의 추가로 여러 통계자료를 가공해 낼 수 있게 되었다. 본 연구는 AWS의 교육적 활용에 중점을 두고 이루어졌으며 간편하고 실용적인 면에서 매우 유용하리라 생각된다. Automatic Weather Systems (AWS) were placed at many educational as well as governmental institutes for the measurement of weather in Korea. However, weather information from AWS was not used as a real time system because of the complexity of the web display. For the web display ;ud automatic store of weather data to be used as a real time system, KNU Weather Now-V1.0 was developed. The system is very simple but useful for students and other users. Thus, everybody can use stored weather data and can process the data easily. This study focuses on the development of the system and the educational usage of AWS.

안과에 의뢰된 신체 감정 환자들의 임상 양상 분석

김현진,이현주,임기환,Hyun-Jin Kim,M,D,Hyun-Joo Lee,M,D,Key-Hwan Lim,M,D 대한안과학회 2008 대한안과학회지 Vol.49 No.5

Purpose: To investigate the clinical characteristics and disability rates of patients seeking expert consultation for legal reasons because of ophthalmologic symptoms. Methods: We analyzed the gender, age, reason for consultation, and result of consultation, among other factors, by retrospectively examining the medical files of 89 patients from January 1999 to December 2004. Results: Out of 89 patients, men constituted 76.4% and patients in their forties constituted 23.0%. The most common reason for consultation was a traffic accident (64.0%), followed by a medical lawsuit (11.2%). Forty-nine patients (55.1%) were diagnosed with a disability, while 30 patients (33.7%) were not. Diagnostic decisions for the other 10 patients (11.2%) were deferred. Optic atrophy was the most frequent cause for deteriorating visual acuity in patients, accounting for 19 cases, and diplopia caused by orbital wall fracture was also prevalent (9 patients). Among the resulting types of damage, brain damage was the most frequent, while the percentage of patients with an impairment in the visual system was approximately 29.0% among the patients diagnosed with a disability. Conclusions: Our results will be helpful in elucidating the clinical characteristics of patients referred to the ophthalmic clinic for consultation because of legal concerns.

내경계막 제거술 후 광간섭단층촬영에서 나타난 황반부 형태의 변형에 대한 분석

김현진,이정희,Hyun-Jin Kim,Jeong-Hee Lee 대한안과학회 2008 대한안과학회지 Vol.49 No.2

`Purpose: To analyse the postoperative anatomical changes of maculae using optical coherent tomography after internal limiting membrane peeling during vitrectomy. Methods: A retrospective review of 22 patients (22 eyes) who underwent vitrectomy with internal limiting membrane peeling was performed. Age, sex, fundus findings, BCVA, operation methods, complications, and postoperative OCT findings were recorded. Results: Of 22 eyes, 12 eyes had epiretinal membranes, 5 eyes had macular holes, and 5 eyes had diffuse diabetic macular edemas. Eighteen of 22 eyes underwent internal limiting membrane peeling using ICG, with 1 eye treated with triamcinolone and 3 eyes treated with nothing. The postoperative improvement of BCVA was statistically significant in epiretinal membrane and diffuse macular edema patients (p<0.05), but insignificant in macular hole patients (p>0.05). Postoperative OCT findings of 17 eyes showed that they had returned to normal shape and thickness. However, among the 18 eyes that underwent ICG dye-assisted ILM, 3 eyes showed irregular macular thinning and 1 eye showed macular cystic changes. One eye treated with triamcinolone presented with a recurrent cytoid macular edema. No postoperative complications or recurrences were reported by epiretinal membrane patients. Conclusions: Changes to macular shape did not significantly affect the final BCVA after internal limiting membrane peeling, but they can cause changes to the microstructure of maculae. Therefore, caution during surgery and close follow-up are necessary.`