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김영아 한국가족치료학회 1993 가족과 가족치료 Vol.1 No.1
이 글에서는 간단하게나마 이러한 질문등에 대한 대답을 찾고자 시도하였다. 또 이러한 여성들의 한을 우리전통사회에서는 어떻게 치료하여 왔으며, 이러한 치유방법이 오늘날 가족치료자들에게는 어떤 의미를 부여하며, 그 치유과정은 어떠한가를 살펴보고자 한다.
복막투석 복막염의 원인균 검출을 위한 16S rRNA 유전자 표적 차세대 염기분석법의 적용
김영아,강이화,문혜수,김대원,용동은 대한임상미생물학회 2020 Annals of clinical microbiology Vol.23 No.1
Background: 16S rRNA gene-targeted next-generation sequencing (NGS) can detect microorganisms in a comprehensive reference database. To date, NGS has been successfully applied to samples such as urine, blood, and synovial fluid. However, there is no data for continuous ambulatory peritoneal dialysis (CAPD) fluid. The purpose of this study was to evaluate the clinical usefulness of microbiome analysis of CAPD fluids for the diagnosis of CAPD peritonitis. Methods: We included 21 patients with high suspicion of CAPD peritonitis. Routine CAPD fluid culture was performed using a pellet of 50 mL CAPD fluid onto the chocolate and blood agar for two days, and thioglycollate broth for one week. 16S rRNA gene-targeted NGS of pellets, stored at -70°C was performed with MiSeq (Illumina, USA). Results: Many colonized or pathogenic bacteria were detected from CAPD fluids using NGS and the microbiomes were composed of 1 to 29 genera with a cut-off 1.0. Compared to the culture results, NGS detected the same pathogens in 6 of 18 valid results (three samples failed with low read count). Additionally, using NGS, anaerobes such as Bacteroides spp. and Prevotella spp. were detected in six patients. In two of five samples in which no bacterial growth was detected, possible pathogens were detected by NGS. Conclusion: To our knowledge, this is the first report about the application of 16S rRNA genetargeted NGS for diagnosis of CAPD peritonitis. Etiology of culture-negative CAPD peritonitis can be better defined in NGS. Furthermore, it also helped the detection of anaerobic bacteria.
김영아,변정현,손영숙,신동천,이경원 대한임상미생물학회 2020 Annals of clinical microbiology Vol.23 No.1
Background: The weather has well-documented effects on infectious disease and reports suggest that summer peaks in the incidences of gram-negative bacterial infections among hospitalized patients. We evaluated how season and temperature changes affect bloodstream infection (BSI) incidences of major pathogens to understand BSI trends with an emphasis on acquisition sites. Methods: Incidence rates of BSIs by Staphylococcus aureus, Enterococcus spp., Escherichia coli , Klebsiella pneumoniae , Acinetobacter spp., and Pseudomonas aeruginosa were retrospectively analyzed from blood cultures during 2008–2016 at a university hospital in Seoul, Korea according to the acquisition sites. Warm months (June–September) had an average temperature of ≥20 °C and cold months (December–February) had an average temperature of ≤5 °C. Results: We analyzed 18,047 cases, where 43% were with community-onset BSI. E. coli (N = 5,365) was the most common pathogen, followed by Enterococcus spp. (N = 3,980), S. aureus (N = 3,075), K. pneumoniae (N = 3,043), Acinetobacter spp. (N = 1,657), and P. aeruginosa (N = 927). The incidence of hospital-acquired BSI by Enterococcus spp. was weakly correlated with temperature, and the median incidence was higher during cold months. The incidence of community-onset BSI by E. coli was higher in warm months and was weakly correlated with temperature. Conclusion: We found seasonal or temperature-associated variation in some speciesassociated BSIs. This could be a useful information for enhancing infection control and public health policies by taking season or climate into consideration.
김영아,김도균,용동은,이경원 대한임상미생물학회 2016 Annals of clinical microbiology Vol.19 No.4
Background: The recovery of bacteria from blood can be affected by many factors. Standardization of blood culture methods is important for reliability. Herein, we aimed to investigate blood culture protocols in Korea. Methods: We performed a multicenter survey with a questionnaire about blood culture practices, which was sent by email to directors and clinical physicians in charge of clinical microbiology laboratories in May 2014. Total data from 18 participating hospitals were analyzed to be used as current baseline data, which is necessary to optimize blood culture protocols. Results: Many laboratories included recommended blood volume, which is a major factor for bacteria recovery rate. This varied across participating laboratories. For adults, blood sampling of 10 mL was recommended by 10 laboratories and 20 mL was recommended by 5 laboratories. For children who weighed 14-36 kg and less than 14 kg, blood sampling of 10 mL (n=8) and 5 mL (n=7) was recommended, respectively. For neonates, less than 1 mL was recommended by 12 laboratories. Conclusion: Substantial variations in blood culture protocols were seen across participating clinical microbiology laboratories. Efforts to standardize this protocol should be undertaken.