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상박신경총 차단에 있어서 Bupivacaine 의 용량 변화에 따른 임상적 고찰
김영대,이홍식,김필곤,엄인호,신건선 대한마취과학회 1989 Korean Journal of Anesthesiology Vol.22 No.4
Brachial plexus block is frequently used for surgery on the upper extremity. Unsuccessful brachial plexus block is usually caused either by injection outside the neurovascular sheath or by incomplete blockade inspite of injection within the neurovascular sheath. Studied by Winnie and Collines suggested that the extent of blockade following injection in the sheath surrounding the brachial plexus also should be directly proportional to the volume of local anesthetic injected. We therefore investigated the extent of blockade using different volume of bupivacaine with supraclavicular approach. The results were as follows. 1) Complete analgesia was observed between the group of 15 ml and 30 ml. 2) The interval of complaint of pain after a single injection ranged from 14.3 to 16.4 hours. Insignifi-cant difference was found between the group of 15 ml and 30 ml (p$gt; 0.1) 3) There was no hematoma, shivering, but there was Horner's syndrome in 13, phrenic N.paralysis in 2, pneumothorax in 1 cases. No general seizure or other side effects were observed. Therefore we come to the conclusion that above the volume of 15 ml is sufficient to brachial plexus block with supraclavicular approach.
동해안 긴잎돌김(Porphyra pseudolinearis)의 성성숙기 특성
김영대,김형근,이주,홍용기,Kim, Young-Dae,Kim, Hyung-Geun,Lee, Ju,Hong, Yong-Ki 한국생명과학회 2003 생명과학회지 Vol.13 No.3
동해안에 있어 긴잎돌김은 우점종으로 조간대 상부지역에 서식하며 10월에 발생하여 3월에 소멸하였다. 10월에 영양세포로 육안 및 조직 절단에 의한 검색에서 성숙되지 않았다. 11월 중순경부터 육안으로는 암.수배우체가 구분되지 않았으나 조직 절단한 결과, 조정기와 조과기을 형성하고 있었다. 12월 초순부터 육안으로 암.수배우체가 확연히 구분되기 시작하였고, 2월경부터 성숙과 더불어 정자낭과 과포자낭의 방출로 점차 엽장의 길이가 줄어드는 추세를 보였다. 3월 초순경에는 색상이 퇴색되고 엽체 가변부에 녹아 내리는 현상이 나타나기 시작하여, 정자방출이 완료되었다. 12월 3일에 암컷의 평균 엽장은 113.0$\pm$6.8mm, 평균 엽폭은15.3$\pm$1.3mm로 나타났다. 수배우체의 평균엽장은 83.2$\pm$5.4mm, 평균엽폭은 16.8$\pm$1.9mm로 나타났다. 1월 28일 경에는 암배우체의 평균 엽장은 94.6$\pm$6.4mm로 엽폭은 29.1mm$\pm$5.1로 커졌다. 수배우체의 경우에 평균 엽장이 107.8$\pm$7.3mm, 평균 엽폭은 25.9$\pm$0.9mm로 엽장과 엽폭이 반비례되는 현상이 나타났다. The seaweed Porphyra pseudolinearis as a dominant species grows at upper of the intertidal zone of the East Sea from October to March. Vegetative cells have not matured during October when observed with naked eye and microscope. In the middle of November, although it didn't distingush between female and male gametophytes by eye, it showed antheridium and carpogonium when observed vertical section under microscope. From early December, It could be distinguished female and male gametophytes clearly. From Feburary it showed shorted length of thalli by release of spermatangia and carposporangia as maturation. At early March, the colar has been decaeded and side of thalli has been melted, completed of release of spermatia. At the end of December, the average length and width of female thalli were 149.9$\pm$5.6mm and 22.2$\pm$2.3mm, respectively. The length and width of male thalli were 149.9$\pm$9.4mm and 20.7$\pm$1.8mm. At the end of January, the average length and width of female thalli were 94.6$\pm$6.4mm and 29.1mm$\pm$5.1, respcetively. The length and width of male thalli were 107.8$\pm$7.3mm and 25.9$\pm$0.9mm. From this period lengths of female and male thalli have already been shortned by the release of spermatia and carpogonia.
한국산 해산 홍조류 긴잎돌김(Porphyra pseudolinearis Ueda)에서의 원형질체 분리
김영대,최재석,이주,손용수,홍용기,Kim, Young-Dae,Choi, Jae-Seok,Lee, Ju,Son, Yong-Soo,Hong, Yong-Ki 한국생명과학회 2003 생명과학회지 Vol.13 No.4
한국 동해안 특산종인 긴잎돌김의 원형질체 분리를 위한 적정한 조건을 구명하기 위한 연구가 이루어 졌다. 홍조류 긴잎돌김은 동해안에 있어 토착종이며 우점종중 하나이다. 원형질체는 2% agarase and 2% hemicellulase 의 효소를 이용하여 25mM Mes buffer, pH 6.0의 0.5M 솔비톨에서 분리되어 졌다. 분리되어진 원형질체는 PEG 8000에서 염색한 오카무라둥근돌김과 접합하였다. Optimal conditions of protoplast isolation from the seaweed Prophyra pseudolinearis have been described. The P. pseudolinearis is one of indigenous and dominant Porphyra species in the East Sea region. Protoplasts have been released by enzymatic treatment of 2% agarase and 2% hemicellulase in 25mM MES buffer, pH 6.0 containing 0.5 M sorbitol. The protoplasts could be fused with neutral red-stained protoplasts of P. okamurae by the addition of polyethylene glycol 8000 solution.