DEAE - Cellulose 에 의한 casein 의 분별에 관한 검토

김영교,신동철,전우민 한국낙농학회 1983 韓國酪農學會誌 Vol.5 No.3

This paper is described on the method for fractionation of casein mixtures by the DEAE-Cellulose column chromatography. For the fractionation of casein mixtures, the method used was ion exchange chromatography on the DEAE-Celluose, Whatman DE52, with tris-chloride-urea buffer (PH 8.6), and a NaCl gradient. The effects of the column length, NaCl concentration and alkylation of the sample were tested for the fractionation of casein mixtures by DEAE-Celluose column chromatography. The results were summarized as follows; 1. By the long column (1.5 × 50 ㎝), γ-casein was fractionated into two peaks, and by the short column (1.5 × 25 ㎝), γ-casein was eluted with a single peak. 2. When the sample was applied to the column and eluted with about 100 ㎖ of the buffer (above bed volumn) containing 0.03 M NaCl, the pure γ-casein was isolated without the contamination of κ-casein. 3. γ-Casein, κ-casein, β-casein, and αs-casein were eluted at 0-0.03 M, 0.04-0.12 M, 0.13-0.15 M and 0.16-0.24 M NaCl concentration, respectively. 4. The fractionation of κ-casein and minor α-casein were improved by the alkylation of casein mixtures.

담쟁이

김영교 문예운동사 2009 문예운동 Vol.- No.104

Casein micelle 의 조성에 관한 연구 : I. Micelle 크기 별에 따른 각 casein 의 구성비율 I. The relative amounts of individual caseins in the different casein micelles

김영교,신동철,전우민 한국낙농학회 1984 韓國酪農學會誌 Vol.6 No.1

本 實驗은 casein miceile의 크기에 따른 각종 casein의 構成成分을 究明하고자 超遠心分離機를 利用하여 casein micelle을 크기 別로 分類하고 DEAE-cellulose column chromatography를 이용하여 각 casein의 構成比率을 조사하였다. 그 結果를 要約하면 다음과 같다. 1. 脫脂乳를 100,000×g에서 각각 30分, 60分, 90分동안, 200,000×g에서 60分동안 遠心分離하였을 때 沈澱되는 casein micelle 量은 45.00%, 61.43%, 80.00% 및 95.00%였으며 沈澱되지 않는 serum casein量은 55.00%, 38.57%, 20.00% 및 5.00%였다. 2. 脫脂乳를 100,000×g에서 時間 差에 따라서 遠心分離하였을 때 각각 沈澱되는 casein micelle量은 10分間에는 全 casein量의 23.13%, 11∼30分間에는 30.26%, 31∼60分間에는 28.11%였으며, 60分問의 遠心分離에 의해서 沈澱되지 않는 serum casein은 17.79%였다. 3. 時問 差로 遠心分離하여 얻어진 casein micelle중 각 casein의 構成比率을 測定한 結果, α_s-casein, β-casein, κ-casein 및 γ-casein의 상대적인 量은 100,000×g, 10分의 pellet에서 각각 54%, 29%, 11% 및 6%였고, 100,000×g, 11∼30分의 pellet에서는 각각 54%, 28%, 13% 및 5%였고, 100,000×g, 31∼60分의 pellet에서는 각각 51%, 28%, 16% 및 5%로 나타났으며, serum casein은 44%, 29%, 19% 및 8%로 각각 나타났다. This experiment was carried out to study on the protein composition of casein micelles. Three micellar casein pellets and a serum casein were isolated at diferent time of ultracen trifugation. And the composition of individual caseins for the fractions was determined by DEAE-cellulose column chromatography. The results are summarized as follows: 1. When skimmilk was centrifuged for 30 min, 60 min and 90 min at 100,000g and for 60 min at 200,000g, the values for the sedimented casein micelles were 45.00%, 61.43%, 80.00% and 95.00% respectively and that of serum casein was 55.00%, 38.57%, 20.00% and 5.00% respectively. 2. When skimmilk was centrifuged for different time at 100,000g, the values for the sedimented casein micelles were 23.18% for 10 min.(pellet 1), 30.26% from 11 to 30 min.(pellet 2), 28.11% from 31 to 60 min.(pellet 3) and that of serum casein which was not sedimented at 60 min was 17.79% of the total casin. 3. The relative amounts of α_s-, β-, k- and γ-caseins in casein micelles obtained after different time of centrifugation were 54%, 29%, 11% and 8% in pellet 1, 54%, 28%, 13% and 5% in pellet 2, 51%, 28%, 16% and 5% in pellet 3,44%, 29%, 19% and 8% in serum casein respectively.

우유 단백질

김영교,Kim, Yong-Kyo 한국식품과학회 1972 한국식품과학회지 Vol.4 No.3

문학 : 시인론 ; 멸집(滅執)과 만공(滿空)의 시학 -시인 고영섭론-

김영교 한국불교사연구소 2006 문학/사학/철학 Vol.6 No.-

유도무기체계 운용시험평가시 M&S 적용방안에 대한 연구- 현궁사업 중심으로 -

김영교,강지훈 한국방위산업학회 2013 韓國防衛産業學會誌 Vol.20 No.1

Due to the high demand for more complex and technical weapon systems, the higher status of Test and Evaluation (T&E) organization has to develop highly objective, scientific and internationally recognized methods throughout the weapon system acquisition process. An example of this can be made of the guided weapon system, which has intellectual and complex functions and can easily become a high-cost project. The military in advanced nations adopt Modeling and Simulation (M&S) in order to minimize Research and Development (R&D) cost, incertitude and risk. The USA has been using both M&S and VV&A (Verification, Validation, and Accreditation) in order to maintain regulations, directives and manuals. In South Korea, however, there are currently no systems to facilitate the application of M&S. In this study, I have tried generating several test items wherein M&S can be applied to ongoing projects of infantry middle range guided weapon (Hyun-gung) and I also suggested a proper M&S method that is currently being applied. Among the M&S methods is the operational effective analysis model, which has been used by the US Army OT (Operational Test). It is considered 'suitable' to measure operational effectiveness and it is a necessary element for the future operational T&E. The procedure of VV&A, which is the key to obtaining credibility, should also be applied to other weapon system's R&D. It will be very beneficial to the increase of the domestic T&E system level.

Effects of ibuprofen-loaded TiO2 nanotube dental implants in alloxan-induced diabetic rabbits

김영교,김완태,정보현,유기연,엄흥식,장범석,이재관,최원열 대한치주과학회 2021 Journal of Periodontal & Implant Science Vol.51 No.5

Purpose: Some systemic conditions, especially diabetes mellitus (DM), adversely affect dental implant success. This study aimed to investigate the effects of ibuprofen-loaded TiO2 nanotube (ILTN) dental implants in alloxan-induced diabetic rabbits. Methods: Twenty-six New Zealand white rabbits were treated with alloxan monohydrate to induce DM. At 2 weeks following DM induction, 3 types of implants (sandblasted, large-grit, and acid-etched [SLA], ILTN, and machined) were placed into the proximal tibia in the 10 rabbits that survived following DM induction. Each type of implant was fitted randomly in 1 of the holes (round-robin method). The animals were administered alizarin (at 3 weeks) and calcein (at 6 weeks) as fluorescent bone markers, and were sacrificed at 8 weeks for radiographic and histomorphometric analyses. Results: TiO2 nanotube arrays of ~70 nm in diameter and ~17 μm in thickness were obtained, and ibuprofen was loaded into the TiO2 nanotube arrays. A total of 26 rabbits were treated with alloxan monohydrate and only 10 rabbits survived. The 10 surviving rabbits showed a blood glucose level of 300 mg/dL or higher, and the implants were placed in these diabetic rabbits. The implant stability quotient (ISQ) and bone-to-implant contact (BIC) values were significantly higher in the ILTN group (ISQ: 61.8, BIC: 41.3%) and SLA group (ISQ: 62.6, BIC: 46.3%) than in the machined group (ISQ: 53.4, BIC: 20.2%), but the difference in the BIC percentage between the SLA and ILTN groups was not statistically significant (P=0.628). However, the bone area percentage was significantly higher in the ILTN group (78.0%) than in the SLA group (52.1%; P=0.000). Conclusions: The: ILTN dental implants showed better stability (ISQ) and BIC than the machined implants; however, these values were similar to the commercially used SLA implants in the 2-week diabetic rabbit model.

아이스크림의 種類와 品質

金榮敎 한국식품과학회 1974 식품과학과 산업 Vol.7 No.3

린드레스치이스의 이점과 문제점

김영교 한국식품산업협회 1978 좋은식품 Vol.43 No.-

한국인의 영양수준과 우유의 영양가치

김영교,Kim, Yeong-Gyo 한국유가공협회 1980 牛乳 Vol.1 No.2