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류정희,곽종환,지옥표,이강노,Lew, Jung-Hie,Kwak, Jong-Hwan,Zee, Ok-Pyo,Lee, Kang-Ro 한국생약학회 1998 생약학회지 Vol.29 No.2
Four flavonoids were isolated from the whole plant of Kyllinga brevifolia var. leiolepsis. Their structures were identified as quercetin, (-)-epiafzelechin, vitexin and orientin on the basis of spectral data. This is the first report of the identification of quercetin, (-)-epiafzelechin and orientin from Kyllinga genus.
김대근,곽종환,송기원,권학철,지옥표,이강노,Kim, Dae-Keun,Kwak, Jong-Hwan,Song, Ki-Won,Kwon, Hack-Cheol,Zee, Ok-Pyo,Lee, Kang-Ro 한국생약학회 1996 생약학회지 Vol.27 No.1
Four steroids and one flavonol glycoside were isolated from the ethanol extract of the whole plant of Aconitum pseudolaeve var. erectum. Their structures were identified as ${\beta}-sitost-4-en-3-one$, 22-dihydro-stigmast-4-en-3,6-dione, ${\beta}-sitosterol$, ${\beta}-sitosterol-3-O-{\beta}-D-glucopyranoside$ and $kaempferol-3-O-{\beta}-D-glucopyranoside(astragalin)$ on the basis of spectral data.
김대근,곽종환,류정희,권학철,송기원,강삼식,이성행,이은아,권년수,이강노,지옥표,Kim, Dae-Keun,Kwak, Jong-Hwan,Ryu, Jung-Hee,Kwon, Hak-Chul,Song, Ki-Won,Kang, Sam-Sik,Lee, Sung-Haeng,Lee, Eun-Ah,Kwon, Nyoyn-Soo,Lee, Kang-Ro,Zee, Ok-Pyo 한국생약학회 1996 생약학회지 Vol.27 No.2
Hydrogen peroxide is one of major chemicals mediating antitumor and antimicrobial activities of macrophages. We searched natural products enhancing hydrogen peroxide generation from murine macrophage-like cell line J774. Among 21 methanol extracts of Korean medicinal plants, the extract from Cornus officinalis was the most effective. The active component from the fractions was searched by activity guided fractionation, and identified as ursolic acid by spectral data.
국내 자생식물의 화학성분 검색(Ⅰ) : 쐐기풀과, 노박덩굴과, 갈매나무과, 벽오동과, 꼭두서니과에 대하여
유승조(Seung Jo Yoo),곽종환(Jong Hwan Kwak) 한국생약학회 1989 생약학회지 Vol.20 No.3
The presence of saponin, terpenoid, steroid, anthraquinone and flavonoid were screened with the usual test methods in forty five(sixty four parts) Korean plants belonging to Urticaceae, Celastraceae, Rhamnaceae, Sterculiaceae and Rubia ceae. Plants were extracted with methanol and fractionated with hexane, chloroform, ethyl acetate and butanol. Each fraction was tested for the components. The result showed that in saponin test, 21 plants were positive and 11 plants were weak positive; in terpenoid and steroid test, 26 plants were strong positive; in anthraquinone test, 20 plants strong positive: in flavonoid test, 31 plants were positive and 9 plants were weak positive.
김대근(Dae Keun Kim),곽종환(Jong Hwan Kwak),권학철(Hack Chol Kwon),송기원(Ki Won Song),지옥표(Ok Pyo Zee),이강노(Kang Ro Lee) 대한약학회 1996 약학회지 Vol.40 No.4
From the ethanol extract of the whole plant of Aconitum pseudo-laeve var. erectum Nakai, three new aromatic amides, methyl-N-acetyl anthranilate, methyl-N-(3-ethoxycarbonylpropionyl)anthranilate, methyl-N-(3-methoxycarbonylpropionyl) anthranilate were isolated and characterized on the basis of spectral data.
유승조(Seung Jo Yoo),송경식(Kyung Shik Song),곽종환(Jong Hwan Kwak),한병훈(Byung Hoon Han),류재하(Jae Ha Ryu) 한국생약학회 1989 생약학회지 Vol.20 No.3
From the root of Rhamnella frangulioides(=Microrhamnus frangulioides), three compounds (Comp. I ; mp 140∼142°, Comp. II; mp 196°, Comp. III ; mp 136∼138°) were isolated by silica gel column chromatographic purification which were identified as β-sitosterol, chrysophanol, 1-methyl-2-carboxymethyl-3-methoxy-4, 8-dihydroxy anthraquinone, respectively, by spectral analysis.
매자나무중 Berberine함량의 계절적 변화에 관한 연구
유승조(Seung Jo Yoo),이경북(Kyung Bok Lee),곽종환(Jong Hwan Kwak) 한국생약학회 1986 생약학회지 Vol.17 No.2
The determination of berberine in Berberis koreana was examined by the high performance liquid chromatography using u-Bondapak C<sub>18</sub> column and CH₃CN/phosphate buffer (pH 5.2) (60/40) as a mobile phase. The seasonal variations of berberine contents in Berberis koreana were as follows; 1) In roots, the average berberine content was 0.94% with the highest level of 1.32% in October. 2) In stems, their average berberine content was about 0.1% and in March, April, October and November, the contents were relatively high. 3) In leaves, however, the content was as low as 0 005%. According to the experimental results obtained, we found that Berberis koreana roots can serve as the useful natural plant resources for the berberine and October is the optimal season for the collection.
이강노(Kang Ro Lee),지옥표(Ok Pyo Zee),곽종환(Jong Hwan Kwak),김영식(Yeong Shik Kim),박호군(Ho Kun Park),구경아(Kyong Ah Koo),윤현주(Hyun Joo Youn) 한국생약학회 1994 생약학회지 Vol.24 No.4
Polysaccharides fractions from the leaves of Artemisia selengensis(ASP1) and Artemisia iwayomogi(AIP1) were extracted and purified by Sephadex gel filtration and DEAE-Sephadex ion exchange chromatographies. Both ASP1 and AIP1 fractions were tested for their effects on the spleen cell culture in vitro. Both ASP1 and AIP1 fractions allow growth of spleen cells up to 3 months in culture, suggesting the immunoregulatory activities of those polysaccharide fractions. The molecular weights of ASP1 and AIP1 fractions were found to be about 2,500 daltons by Sephadex gel filtration chromatography using standard dextrans. Both ASPl and AIPl fractions were composed of glucose, xylose and galactose.