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      • 설치류의 핵형에 관한 연구 : Sprague-Dowley계 백서 골수세포의 염색체

        申泰善 中央醫學社 1974 中央醫學 Vol.26 No.5

        A cytogenetic study having been carried out by culturing the bone marrow of the albino rat, Sprague-Dowley strain, its normal chromosome complement proved to be composed of 42 chromosomes ; 20 pairs of autosomes, and one pair of sex chromosomes, XX or XY. Twelve pairs of autosomes, number 1 through 12, and the sex chromosomes, X & Y, were acrocentrics, but the other eight pairs of autosomes, number 13 through 20, were metacentrics or submetacentrics. Since the X chromosome was very similar to the number 2 chromosome and the Y, to the number 17, these chromosomes could hardly be clarified. The karyotype of the animal was compared and discussed with that of the Wistar-strain rat.

      • 염색체의 대상구조에 관한 연구 : Sprague-Dowley계 흰쥐의 G-banding G-banding Pattern of Albino Rat, Sprague-Dowley Strain

        申泰善,崔憲植 中央醫學社 1975 中央醫學 Vol.28 No.2

        By the Trypsin-Giemsa method, with 0.025 0, 0.05% or 0.25% trypsin solution which was dissolved in Ca & Mg-free Hanks' solution or in physiological saline, the G-banding was demonstrated in bone marrow cells of the normal albino rat, Sprague-Dowley strain which had been held in this department, and a tentative numbering system for the chromosomal complement of the strain was proposed (Fig. 2). Its normal chromosome set was comprised of 42 chromosomes; 20 pairs of autosomes, and-one pair of sex chromosomes, XX or XY chromosomes. The longer their total lengths were, the more numerous their number of bandings tended to be, and Y chromosomes were characterized by their special feature of bandings. The polymorphism of chromosomes was suggested.

      • 넙치 및 조피볼락용 습사료의 보관조건에 따른 안정성 평가

        안창범,주용석,정관식,서경란,신태선 여수대학교 1998 論文集 Vol.13 No.2

        본 실험은 습사료를 대상으로 보관조건(온도별, 시간별)에 따른 지질의 산화진행 정도를 파악하여 사료의 효율적인 이용성을 구명하고자 산가(acid value, AV), 과산화물가(peroxide avlue, POV) 및 비타민 함량을 분석하였다. 생사료와 분말배합사료를 혼합하여 제조한 습사료는 혼합비가 8:2 사료에서 5:5 사료보다 높은 AV와POV를 나타내었고, 보관기간이 경과함에 따라 보관조건과는 상관없이 8:2 사료에서 빠른 산패를 보였다. 산화진행속도는 4℃ 보관조건하에서는 48시간째, -15℃ 보관조건하에서는 72시간째에서 빠르게 일어났다. 60,000 Lux이상의 직사광선 노출하에서의 AV,POV는 노출시간이 길어질수록 증가하였고, 8:2사료에서 빠르게 변화하였다. This experiment was conducted to investigate rancidity in moist pellet(MP) during various practical condition of handling and storage conditions. The experimental moist pellet diets were prepared by mixing frozen raw fish (FRF) and commercial compound meal (CCM) in ratio of 8:2 and 5:5, respectively. Immediately before and after manufacturing of MP, the MPs were stored at 4℃ and -15℃, and exposed under the sunlight(30±2℃, 60000Lux). The rancidity of each MP was determined from 1 to 96 hours after pellting. The acid value (AV) and peroxide value (POV) in the diets stored at 4 and -15℃ increased rapidly after 48 and 72 hours, respectively. For the sample exposed sunlight, AV and POV were slightly increased with the exposed time. The rancidity increased in the 8:2MP(FRF : CCM) than in the 5:5MP(ERF : CCM) at all storage condition and the amount of vitamin E in MPs decreased rapidly as AV and POV increased.

      • 참치자즙(煮汁) 분말엑기스를 이용한 조미간장의 제조

        안창범,김형락,신태선 여수대학교 1998 論文集 Vol.13 No.2

        참치자숙액을 원료로 분말엑기스를 제조하여 그 기능특성을 살펴보고, 분말에기스를 주원료로 하는 조미간장을 제조하여 시판간장과의 관능적인 평가에 의하여 그 품질을 비교, 검토하였다. 분말엑기스의 용해도는 100%였다. 분말화한 원료자즙과 분말엑기스의 유화성은 각각 54.0%, 47.7%로 큰 차이는 없었지만 분말엑기스의 유화안정성은 76.6%로 분말화한 원료자즙에 비해 많이 떨어졌다. 포말성과 포말안정성은 분말화한 원료자즙의 경우 약간 있는 정도이고 분말엑기스의 경우는 거의 없었다. 물 100 ml에 식염과 분말엑기스를 각각 5.0 g을 녹인 용액에 시판 양조간장을 10%(v/v) 또는 20%(v/v)를 혼합하여 만든 조미간장은 시판 3종류의 화학간장과 관능적으로 손색이 없었다. The imitation sauce was prepared by using the extract of skipjack cooking juice and its product quality was also compared with three kinds of soy sauce on the market sensually. The extract powder was prepared from a skipjack cooking juice by hydrolyzing with 0.5% protease at 50℃, treating with charcoal, filtering under reduced pressure and drying by spray-dryer. Solubility and emulsifying capacity of the extract powder was 100.0% and 47.4%. From the results of sensory evaluation, the imitation sauce prepared by dissolving the extract powder 5.0g, NaCl 5.0g and a fermented sauce 20ml in water 80 ml was at least equal to three kinds of soy sauce on the market in product quality.

      • SCOPUSKCI등재

        이식 탈회골의 골화에 대한 실험적 연구

        구순상,이종원,이택호,신태선 大韓成形外科學會 1984 Archives of Plastic Surgery Vol.11 No.2

        The most effective osseous graft material is the fresh autogenous bone in clinical practice and experimental studies. However, there are some problems in use of fresh autogenous bone graft, such as donor-site morbidity and the limitation in amount of available autogenous bone. Since the introduction of decalcified bone as grafting material by Senn(1889), decalcified bone has been studied as substitute for autogenous bone. The implantation of decalcified bone segment or powder in soft tissue has been established as a laboratory model for studying bone induction. Bone powders (particle size: 74∼420㎛) of rat and ox were decalcified with 0.5M HCI and were implanted i rat's abdomen subcutaneously. Thereafter, periodic observation was made histologically up to 30 days. Comparative studies were carried out to determine differences in histological sequence of induced osteogenesis and quantity of new bone. The results were as follows; 1. Implantation of decalcified bone powders of rat and ox to subcutaneous site in rats resulted in new bone formation by an enchondral sequence and there were no differences in histological change periodically. 2. There were no differences in quantity of new bone.

      • Methylation 방법에 따른 지방산 함량 버교

        신태선 전남대학교 수산과학연구소 2004 수산과학연구소논문집 Vol.13 No.-

        Five different methods (sodium methoxide, methanolic HCl, BF3, Prep 11, TMSH) for methylation of fatty acid was applied to vegetable oils and fish oils and determined a optimum methods for each oil. BF3 method for transesterification of sesame oil was best to convert to fatty acid methyl ester (FAME) from triglyceride, followed by methanolic HCl and Prep 11. The sodium methoxide method could not r?act with free fatty acid to change F AME. The contents of F AME were much different among the five methods. However, the percentage of fa한y acid composition was not different as much as the difference of contents. A suitable method for soybean oil also was BF3 and the next one was Prep 11. The olive oil was the highest FAME contents with methanolic HCl (91.7 g/100 g oil). The methods of Prep 11 and TMSH had similar contents of FAME. Fish oils had different trends from those vegetable oils. The methanolic HCl gave the highest F AME content of a squid oil and followed by sodium methoxide, Prep 11, and BF3. For a walleye pollack oil, the optimum method was TMSH which was the last in the squid oil. The methanolic HCl was considered well react with fish oil. However, the determination of highest contents of FAME in vegetable oils and fish oils was dependent on the selection of optimum method.

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