Improvement of the Steroid Dihydroxylation Efficiency from Dehydroepiandrosterone Using a Substrate Pre-induction Biotransformation Process

Hui Li,Zhenzhen Fu,Heng Li,Wenfang Dou,Jinsong Shi,Zhenghong Xu 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.3

This study investigated the effects of hydroxylase cyptochrome P450 inducers on the efficiency of the biotransformation of dehydroepiandrosterone (DHEA) to 3β, 7α, 15α-trihydroxy-5-androsten-17-one (7α,15α-diOHDHEA)by Colletotrichum lini ST-1. Special attention was given to the substrate DHEA being the best inducer and the fact that it could improve the yield of 7α, 15α-diOHDHEA. Based on the effects of the DHEA pre-induction phases and additional concentrations on 7α, 15α-diOHDHEA production, a substrate pre-induction process was established as follows: 2 g/L DHEA was added for the first time after 12 h of inoculation, followed by the second addition of 6 g/L DHEA after 12 h later. The results showed that this substrate pre-induction process improved the content of cytochrome P450 and that the 7α, 15α-diOH-DHEA yield reached 90.1%, which was 26.9%higher than that obtained in the original process.

Robust Model Predictive Control for Multi-phase Batch Processes with Asynchronous Switching

Hui Li,Lijun Song,Xueying Jiang,Huiyuan Shi,Chengli Su,Ping Li 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.1

A robust asynchronous switching model predictive controller is designed for multi-phase batch processes with uncertainties, unknown disturbances and time-varying set-point. Firstly, in view of the influence of timevarying set-point and disturbances, an asynchronous switching model with stable and unstable cases is established. Based on the switching model, a robust asynchronous switching model predictive control law is designed. Secondly, by using relevant theories and methods, the sufficient conditions with the form of linear matrix inequality (LMI) are given to ensure that the multi-phase batch processes are asymptotically stable at each phase and exponentially stable at each batch. Then, these LMI conditions are solved online to obtain the control gain of each phase, the shortest running time of each stable case, and the longest running time of each unstable case. Finally, the effectiveness and feasibility of the proposed method are verified by taking the injection molding process as an example.

Anti-allergic Effects and Related Active Constituents of Mung Bean (Vignaradiatus Linn) Sprouts

Li Li,Min-hui Li,Han-kun Ren,Yujing Shi,Yin-mao Dong 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.2

The mung bean (Vigna radiata) is an important food crop with preventative effects against human diseases. The anti-allergic activities of mung bean sprouts of different lengths were evaluated by assaying in vivo antipruritic activity and in vitro hyaluronidase inhibitory effects. After 48 h of growth, sprouts were determined to have the best activity and extracted with petroleum (PeF), ethyl acetate (EaF), and n-butanol (nBF). The active EaF extracts were further assayed for in vivo effects on compound 48/80-induced mast cell degranulation and histamine release, as well as the antidinitrophenyl (DNP) IgE-induced passive cutaneous anaphylaxis (PCA) reaction. The main chemical constituents were further analyzed by UV spectrophotometry and high-performance liquid chromatography with tandem mass spectrometric detection (LC/MS/MS). EaF significantly protected against compound 48/80-induced mast cell degranulation and histamine release, and PCA. Flavonoids were determined to be the main contributors to the anti-allergic activity of the EaF extracts.

RECIPROCAL BEHAVIOR OF THE INBOUND GROUP TUORISTS IN TIBET

Li Hui,Yang Zhenzhi,Li Taohong,Shi Hong 글로벌지식마케팅경영학회 2016 Global Marketing Conference Vol.2016 No.7

Based on the reciprocity theory, a dynamic reciprocal behavior model is verified by sampling the US and French group tourists in Tibet. Result shows that the US group tourists have only negative reciprocity, and French ones have both positive and negative reciprocity, indicating that tourists are more willing to sacrifice their interests to revenge hostile persons.

Casein Kinase 2 interacts with human mitogen- and stress-activated protein kinase MSK1 and phosphorylates it at Multiple sites

( Yan Shi ),( Guang Hui Han ),( Hui Ling Wu ),( Kan Ye ),( Zhi Peng Tian ),( Jia Qi Wang ),( Hui Li Shi ),( Ming Liang Ye ),( Han Fa Zou ),( Ke Ke Huo ) 생화학분자생물학회 2009 BMB Reports Vol.42 No.12

miR-140-3p Knockdown Suppresses Cell Proliferation and Fibrogenesis in Hepatic Stellate Cells via PTEN-Mediated AKT/mTOR Signaling

Shi-Min Wu,Tian-Hong Li,Hao Yun,Hong-Wu Ai,Ke-Hui Zhang 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.6

Purpose: Liver fibrosis is a major cause of morbidity and mortality and the outcome of various chronic liver diseases. Activationof hepatic stellate cells (HSCs) is the key event in liver fibrosis. Studies have confirmed that miR-140-3p plays a potential regulatoryeffect on HSC activation. However, whether miR-140-3p mediates the liver fibrosis remains unknown. Materials and Methods: Expression of miR-140-3p was detected by real-time quantitative PCR (qPCR). Cell proliferation wasmeasured by MTT, while cell apoptosis rate was determined via flow cytometry. Western blot assay was used to detect the expressionof cleaved PARP. The fibrogenic effect was evaluated by expression of α-smooth muscle actin and desmin. Functional experimentswere performed in transforming growth factor β1 (TGF-β1)-induced HSC-T6 cells with transfection of anti-miR-140-3pand/or siPTEN. Target binding between miR-140-3p and PTEN was predicted by the TargetScan database and identified usingluciferase reporter assay and RNA immunoprecipitation. Results: TGF-β1 induced the activation of HSC-T6 cells, and miR-140-3p expression varied according to HSC-T6 cell activationstatus. Knockdown of miR-140-3p reduced cell proliferation and the expressions of α-SMA and desmin, as well as increasedapoptosis, in TGF-β1-induced HSC-T6 cells, which could be blocked by PTEN silencing. Additionally, inactivation of the AKT/mTOR signaling pathway stimulated by miR-140-3p knockdown was abolished when silencing PTEN expression. PTEN was negativelyregulated by miR-140-3p via direct binding in HSC-T6 cells. Conclusion: miR-140-3p is an important mediator in HSC-T6 cell activation, and miR-140-3p knockdown suppresses cell proliferationand fibrogenesis in TGF-β1-induced HSC-T6 cells, indicating that miR-140-3p may be a potential novel molecular targetfor liver fibrosis.

Research on training Strategy of information Technology Application Ability of Normal university students

Shi-mei JIN,Liu-wen DING,Ding-rui LIU,Hui-yan LI ASCONS 2020 IJASC Vol.2 No.4

Background/Objectives: Under the background of educational informatization, the realization of education modernization and information education is the inevitable trend of future education development. As the backbone of the future teacher team, the level of information technology application ability of normal university students is the key to the modernization of future education. Therefore, the ministry of education proposed to strengthen the cultivation of information literacy of normal university students. Methods/Statistical analysis: As the cradle of basic education teachers in yanbian prefecture, yanbian university should strengthen the cultivation of information technology application ability of normal university students and study the current situation of the cultivation. In this paper, two questionnaires were conducted before and after the training of normal university students in yanbian university. Findings: Through the comparison and analysis of the two data by SPSS software, it is found that there is no specific training for normal university students in the training of information technology application ability of normal university students in yanbian university. Teaching less theoretical knowledge; Students have fewer opportunities to practice the problem. Improvements/Applications: Finally, the author puts forward some strategies for the future cultivation of information technology application ability of normal university students in yanbian university. For the training of excellent information teachers to contribute.

Selective Inhibition of Bicyclic Tetrapeptide Histone Deacetylase Inhibitor on HDAC4 and K562 Leukemia Cell

Li, Xiao-Hui,Huang, Mei-Ling,Wang, Shi-Miao,Wang, Qing Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Histone deacetylase (HDAC) inhibitors of cyclic peptide have been proved to be the most complex but the most stable and relative efficient inhibitors because of their large cap region. In this paper, a series of studies were carried out to evaluate the efficacy of synthetic bicyclic tetrapeptide inhibitors 1-5 containing hydroxamic acid referring molecular docking, anti-proliferation, morphology and apoptosis. Docking analysis, together with enzyme inhibitory results, verified the selective capability of inhibitor 4 to HDAC4, which might closely related to haematological tumorigenesis, with Phe227, Asp115, Pro32, His198 and Ser114 participating into hydrophobic interactions and Van der Waals force which was familiar with former study. Moreover, inhibitor 4 inhibited K562 cell line at the $IC_{50}$ value of 1.22 ${\mu}M$ which was 51-67 times more efficient than that for U937 and HL60 cell lines. Inhibitor 4 exhibited the cell cycle-arrested capability to leukemia at S phase or G2/M phase as well as apoptosis-induced ability in different degrees. Finally, we considered that bicyclic tetrapeptide inhibitors were promising inhibitors used in cancer treatment and inhibitor 4 could prevent K562 cell line well from proliferation, arrest cell cycle and induce K562 towards apoptosis to achieve the goals of reversing cancer cells which could become a potential leukemia therapeutic agent in the future.

Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage

Li-ming Wu,Rui Guo,Lin Hui,Yong-gang Ye,Jing-mei Xiang,Chun-yun Wan,Miao Zou,Rui Ma,Xiao-zhuan Sun,Shi-jin Yang,Ding-zong Guo 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.4

Chronic enteritis can produce an excess of reactive oxygenspecies resulting in cellular damage. Stanniocalcin-1(STC-1)reportedly possesses anti-oxidative activity, the aim of thisstudy was to define more clearly the direct contribution ofSTC-1 to anti-oxidative stress in cattle. In this study, primaryintestinal epithelial cells (IECs) were exposed to hydrogenperoxide (H2O2) for different time intervals to mimic chronicenteritis-induced cellular damage. Prior to treatment with 200μM H2O2, the cells were transfected with a recombinantplasmid for 48 h to over-express STC-1. Acridine orange/ethidium bromide (AO/EB) double staining and trypan blueexclusion assays were then performed to measure cell viabilityand apoptosis of the cells, respectively. The expression of STC-1and apoptosis-related proteins in the cells was monitored byreal-time PCR and Western blotting. The results indicated thatboth STC-1 mRNA and protein expression levels positivelycorrelated with the duration of H2O2 treatment. H2O2 damagedthe bovine IECs in a time-dependent manner, and this effectwas attenuated by STC-1 over-expression. Furthermore, overexpressionof STC-1 up-regulated Bcl-2 protein expression andslightly down-regulated caspase-3 production in the damagedcells. Findings from this study suggested that STC-1 plays aprotective role in intestinal cells through an antioxidant mechanism.

Value of PAX1 Methylation Analysis by MS-HRM in the Triage of Atypical Squamous Cells of Undetermined Significance

Li, Shi-Rong,Wang, Zhen-Ming,Wang, Yu-Hui,Wang, Xi-Bo,Zhao, Jian-Qiang,Xue, Hai-Bin,Jiang, Fu-Guo Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.14

Background: Detection of cervical high grade lesions in patients with atypical squamous cells of undetermined significance (ASCUS) is still a challenge. Our study tested the efficacy of the paired boxed gene 1 (PAX1) methylation analysis by methylation-sensitive high-resolution melting (MS-HRM) in the detection of high grade lesions in ASCUS and compared performance with the hybrid capture 2 (HC2) human papillomavirus (HPV) test. Materials and Methods: A total of 463 consecutive ASCUS women from primary screening were selected. Their cervical scrapings were collected and assessed by PAX1 methylation analysis (MS-HRM) and high-risk HPV-DNA test (HC2). All patients with ASCUS were admitted to colposcopy and cervical biopsies. The Chisquare test was used to test the differences of PAX1 methylation or HPV infection between groups. Results: The specificity, sensitivity, and accuracy for detecting CIN2 + lesions were: 95.6%, 82.4%, and 94.6%, respectively, for the PAX1 MS-HRM test; and 59.7%, 64.7%, and 60.0% for the HC2 HPV test. Conclusions: The PAX1 methylation analysis by MS-HRM demonstrated a better performance than the high-risk HPV-DNA test for the detection of high grade lesions (CIN2 +) in ASCUS cases. This approach could screen out the majority of low grade cases of ASCUS, and thus reduce the referral rate to colposcopy.