In Vitro Antiviral Activity of Sakuranetin against Human Rhinovirus 3

최화정 질병관리본부 2017 Osong Public Health and Research Persptectives Vol.8 No.6

Objectives: Rhinoviruses (RVs) cause common cold and are associated with exacerbation of chronic inflammatory respiratory diseases. Until now, no clinically effective antiviral chemotherapeutic agents to treat diseases caused by human rhinoviruses (HRVs) have been reported. We assessed the anti-HRV3 activity of sakuranetin isolated from Sorbus commixta Hedl. in human epithelioid carcinoma cervix (HeLa) cells, to evaluate its anti-rhinoviral potential in the clinical setting. Methods: Antiviral activity and cytotoxicity as well as the effect of sakuranetin on HRV3-induced cytopathic effects (CPEs) were evaluated using the sulforhodamine B (SRB) method using CPE reduction. The morphology of HRV3-infected cells was studied using a light microscope. Results: Sakuranetin actively inhibited HRV3 replication and exhibited antiviral activity of more than 67% without cytotoxicity in HeLa cells, at 100 μg/mL. Ribavirin showed anti-HRV3 activity similar to that of sakuranetin. Treatment of HRV-infected HeLa cells with sakuranetin visibly reduced CPEs. Conclusion: The inhibition of HRV production by sakuranetin is mainly due to its general antioxidant activity through inhibition of viral adsorption. Therefore, the antiviral activity of sakuranetin should be further investigated to elucidate its mode of action and prevent HRV3- mediated diseases in pathological conditions.

왕벚나무 껍질로부터 Naringenin, Sakuranetin, Prunetin의 분리 및 함량 분석

정길생,김은남,김성철 한국생약학회 2019 생약학회지 Vol.50 No.3

Prunus yedoensis Matsum. (Rosaceae) has been used for cough, urticaria, pruritus, dermatitis, asthma and relaxation in traditional Korean medicine. In this study, naringenin, sakuranetin and prunetin were isolated from the barks of P. yedoensis and quantification were performed by using high performance liquid chromatography (HPLC) method with diode array detector. The structures of naringenin and sakuranetin, prunetin were identified NMR spectroscopic data analysis. The content of each compound was analyzed by HPLC and the analytical method was validated through linearity, precision, accuracy and specificity test. The result showed that calibration curves of three compounds naringenin, sakuranetin and prunetin indicated great linearity with a correlation coefficient (R²) of 1.00, 1.00 and 0.99. Intra and inter day measurement accuracy of the three compounds ranged from 92.70 ~ 112.70%, and showed precision was less than 3%. Therefore, the content analysis showed that naringenin (0.132 ± 0.016%), sakuranetin (0.108 ± 0.023%), and prunetin (0.059 ± 0.014%).

Inhibitory effect of sakuranetin on (1,3)-β-glucan synthase

You, Myung-Ja,Kim, Bo-Mi,Bhatt, Lok Ranjan,Chai, Kyu-Yun,Baek, Seung-Hwa Kyung Hee Oriental Medicine Research Center 2010 Oriental pharmacy and experimental medicine Vol.10 No.1

An examination of the kinetic properties of UDP-glucose, (1,3)-$\beta$-glucans (callose) synthase, from mung bean seedlings (Sorbus commixta cortex) shows that these enzymes have a complex relationship with UDP-glucose and various effectors. Fluorescence assay showed that deoxynojirimycin increased the inhibitory effect of (1,3)-$\beta$-glucan synthase in a concentration-dependent manner. The inhibitory effect of sakuranetin (34.34%) was higher than that of deoxynojirimycin (80.63%). Disk diffusion method revealed that sakuranetin inhibited the growth of Candida albicans to a 1.5 mm inhibition zone. These results suggest that sakuranetin, isolated from Sorbus commixta cortex extract, can be used as stable antifungal material.

Inhibitory effect of sakuranetin on (1,3)-β-glucan synthase

Myung Ja You,김보미,Lok Ranjan Bhatt,채규윤,백승화 경희대학교 융합한의과학연구소 2010 Oriental Pharmacy and Experimental Medicine Vol.10 No.1

An examination of the kinetic properties of UDP-glucose, (1,3)-β-glucans (callose) synthase, from mung bean seedlings (Sorbus commixta cortex) shows that these enzymes have a complex relationship with UDP-glucose and various effectors. Fluorescence assay showed that deoxynojirimycin increased the inhibitory effect of (1,3)-β-glucan synthase in a concentration-dependent manner. The inhibitory effect of sakuranetin (34.34%) was higher than that of deoxynojirimycin (80.63%). Disk diffusion method revealed that sakuranetin inhibited the growth of Candida albicans to a 1.5 mm inhibition zone. These results suggest that sakuranetin, isolated from Sorbus commixta cortex extract, can be used as stable antifungal material.

Cytotoxic Property of Ultraviolet-induced Rice Phytoalexins to Human Colon Carcinoma HCT-116 Cells

박종화,정인식,Yuan-Yuan Fu,조만호,한태룡 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.2

Exposure to ultraviolet (UV) radiation increased the cytotoxic effect of rice leaf extract on human colon carcinoma HCT-116 cells, suggesting that the production of cytotoxic compounds in rice leaves are induced by UV treatment. To identify cytotoxic agents in UV-treated rice leaves, the phenolic compound peaks prominently increased by UV treatment were isolated using reversed phase high performance liquid chromatography (HPLC). The isolated compounds were identified as N-trans-cinnamoyltyramine, Nbenzoyltryptamine,and sakuranetin using NMR and mass spectrometric techniques. N-Benzoyltryptamine and sakuranetin were previously reported as rice phytoalexins, and N-transcinnamoyltyramine was first isolated from rice. N-Benzoyltryptamine and sakuranetin were found to inhibit the growth of HCT-116 cells with IC50 values of 89.2±4.8 and 68.8±5.2 μg/mL,respectively, indicating that these phytoalexins are cytotoxic agents in the UV-treated rice leaves. These results suggest that rice phytoalexins are potent anti-cancer agents and UV-treated rice leaves are valuable source for therapeutic agents.

Cytotoxic Property of Ultraviolet-induced Rice Phytoalexins to Human Colon Carcinoma HCT-116 Cells

Park, Jong-Hwa,Fu, Yuan-Yuan,Chung, In Sik,Hahn, Tae-Ryong,Cho, Man-Ho The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.2

Exposure to ultraviolet (UV) radiation increased the cytotoxic effect of rice leaf extract on human colon carcinoma HCT-116 cells, suggesting that the production of cytotoxic compounds in rice leaves are induced by UV treatment. To identify cytotoxic agents in UV-treated rice leaves, the phenolic compound peaks prominently increased by UV treatment were isolated using reversed phase high performance liquid chromatography (HPLC). The isolated compounds were identified as N-trans-cinnamoyltyramine, N-benzoyltryptamine, and sakuranetin using NMR and mass spectrometric techniques. N-Benzoyltryptamine and sakuranetin were previously reported as rice phytoalexins, and N-trans-cinnamoyltyramine was first isolated from rice. N-Benzoyltryptamine and sakuranetin were found to inhibit the growth of HCT-116 cells with $IC_{50}$ values of $89.2{\pm}4.8$ and $68.8{\pm}5.2{\mu}g/mL$, respectively, indicating that these phytoalexins are cytotoxic agents in the UV-treated rice leaves. These results suggest that rice phytoalexins are potent anti-cancer agents and UV-treated rice leaves are valuable source for therapeutic agents.

수목추출물의 생리활성에 관한 연구 (XII) : 산벚나무 심재 추출성분의 항균 및 항산화활성 (2) Antimicrobial and Antioxidative Activities of Extractives from the Heartwood of Prunus Sargentii (2)

이학주,이성숙,최돈하 한국목재공학회 2003 목재공학 Vol.31 No.4

전보에 이어 산벚나무(P. sargentii) 심재 에탄올 추출물로부터 4개의 페놀성 화합물을 단리하였으며 기기분석결과, isoflavone인 prunetin를 비롯하여 flavanone인 angophorol, sakuranetin 그리고 flavanone 배당체인 isosakuranin으로 각각 동정하였다. 그러나 이들의 항균 및 항산화 활성을 조사한 결과 활성이 낮아 산벚나무 에탄올 조추출물의 높은 항균 및 항산화 활성은 이들 단리물질과는 무관한 것으로 사료되었다. Four flavonoids were isolated from the heartwood of Prunus sargentii. The structures were identified by NMR spectroscopic analysis: prunetin as isoflavone, angophorol, and sakuranetin as flavanone, and isosakuranin as flavanone glycoside. However, these compounds indicated low antifungal and antioxidative activiteis. In this regard, it could suggest that high antifungal and antioxidative activities of extractives of P. sargentii have no relationship with these compounds.

정공피으로부터 항염증물질의 연구

윤용갑(Young Gab Yun),채규윤(Kyu Yun Chai),록란잔밭(Kyung Kwan Lee),이경관(Lok Ranjan Bhatt),백승화(Seung Hwa Baek) 대한약학회 2009 약학회지 Vol.53 No.6

Sakuranetin, prunetin and dihydroquercetin were isolated from the methanol extract of Sorbus commixta by 1D/2D-NMR and LC-MASS spectrometry. Medicating these compounds to RAW264.7 cell that was pre-treated by lipopolysaccharide revealed anti-inflammatory effects that greatly inhibited the production of NO (nitric oxide) and PEG2 (prostaglandin E2), which are well known to cause the expression of iNOS (inducible nitric oxide synthase) and COX-2 (Cyclooxygenase-2). These results suggest that these compounds can be used as stable anti-inflammatory materials.