유방의 양성 및 악성 유두상 종양에서 Cyclin D1, MIB-1, p53 및 c-erbB-2 발현

김태현,윤기영 고신대학교(의대) 고신대학교 의과대학 학술지 2001 고신대학교 의과대학 학술지 Vol.16 No.1

Background : Histological differentiation between papillary carcinoma from benign papilloma of the breast is difficult in some instances, and papillomas can undergo malignant transformation. The author investigated the expression of cyclin D1, MIB-1 (Ki-67), p53 and c-erbB-2 to assess the diagnostic value of these markers in distinguishing papillary carcinoma from papilloma of the breast. Methods : Immunohistochemical stains for cyclin D1, MIB-1, p53 and c-erbB-2 were performed in 30 cases of papilloma (20 cases of single lesion and 10 cases of multiple lesion) and 8 cases of papillary carcinoma of the breast. Results : Labelling index of cyclin D1 was significantly higher in 8 papillary carcinomas (36.7±27.0) than in 30 papillomas (3.5±4.2) (P=0.001). MIB-1 labelling index was 4.9±5.9 in 8 papillary carcinomas and 0.4±1.4 in 30 papillomas, and there was statistically significant difference (P=0.0225). P53 protein expression rate was significantly higher in 8 papillary carcinomas (87.5%) than in 30 papillomas (30.3%) (P=0.00541). There were no difference of cyclin D1, MIB-1, and p53 expression between solitary intraductal papillomas and multiple papillomas. In all benign and malignant papillary lesions, c-erb-B2 expression was not seen. Conclusions : These results suggest that cyclin D1, MIB-1 and p53 might be useful markers for distinguishing papillary carcinomas from papillomas of the breast Background : Histological differentiation between papillary carcinoma from benign papilloma of the breast is difficult in some instances, and papillomas can undergo malignant transformation. The author investigated the expression of cyclin D1, MIB-1 (Ki-67), p53 and c-erbB-2 to assess the diagnostic value of these markers in distinguishing papillary carcinoma from papilloma of the breast. Methods : Immunohistochemical stains for cyclin D1, MIB-1, p53 and c-erbB-2 were performed in 30 cases of papilloma (20 cases of single lesion and 10 cases of multiple lesion) and 8 cases of papillary carcinoma of the breast. Results : Labelling index of cyclin D1 was significantly higher in 8 papillary carcinomas (36.7±27.0) than in 30 papillomas (3.5±4.2) (P=0.001). MIB-1 labelling index was 4.9±5.9 in 8 papillary carcinomas and 0.4±1.4 in 30 papillomas, and there was statistically significant difference (P=0.0225). P53 protein expression rate was significantly higher in 8 papillary carcinomas (87.5%) than in 30 papillomas (30.3%) (P=0.00541). There were no difference of cyclin D1, MIB-1, and p53 expression between solitary intraductal papillomas and multiple papillomas. In all benign and malignant papillary lesions, c-erb-B2 expression was not seen. Conclusions : These results suggest that cyclin D1, MIB-1 and p53 might be useful markers for distinguishing papillary carcinomas from papillomas of the breast.

Immunohistochemical study on the expression of matrix metalloproteinase 2 and high-risk human papilloma virus in the malignant progression of papillomas

Ho-Jin Lee,Jin-Wook Kim 대한구강악안면외과학회 2013 대한구강악안면외과학회지 Vol.39 No.5

Objectives: Papilloma frequently develops as a benign tumor of the head and neck area, but its potential for malignant transformation has yet to be studied. This study aims to provide basic information for papillomas using the immunohistochemical staining of matrix metalloproteinase 2 (MMP-2) and human papilloma virus (HPV) 16 and 18. Materials and Methods: To evaluate the malignant transformation of papillomas, the selected tissue samples were serially diagnosed with precancerous papilloma (with epithelial dysplasia, pseudo-epitheliomatous hyperplasia) or malignant lesion (squamous cell carcinoma, SCC) after the first diagnosis (squamous papilloma, inverted papilloma). The selected tissues were stained with an antibody to MMP-2 and HPV 16-E7, HPV 18-L1. A statistical analysis was performed according to each transformation step. Results: The epithelial layer of papilloma and pre-cancerous papilloma lesions had a similar MMP-2 expression, but that of the malignant lesion had a significantly increased MMP-2 expression. HPV 16 and 18 infection rates were 28.6%, 33.3% and 63.6% in papillomas, pre-cancerous papilloma lesions, and SCC. Conclusions: A relatively high MMP-2 expression and HPV 16 or 18 infection of papillomas may be associated with early events in the multistep processes of malignant transformation of papillomas.

구강편평세포유두종에서 유두성 유두종, 우췌성 유두종, 내장성 유두종의 감별진단

이상신,김현주,박성연,김연숙,이석근 대한구강악안면병리학회 2012 대한구강악안면병리학회지 Vol.36 No.2

Oral squamous cell papillomas(OSCPs) showed various features in their etiology, histology, and prognosis. Therefore, it is necessary to diagnose differentially according to their pathological examinations. In the present study total 14 cases of OSCPs were evaluated and characterized to be three types of OSCPs, i.e., papillary papilloma, verrucous papilloma, and inverted papilloma. The present study demonstrated that among 14 cases of OSCPs papillary papilloma (n=6) showed the typical papillary projection of squamous epithelium with severe acanthosis of upper spinous layer cells, accompanying frequent nuclear vacuolization. And verrucous papilloma (n=7) showed the diffuse acanthosis of whole spinous layer cells with severe basal hyperplasia, resulted in the thick squamous epithelium exhibiting verrucous surface and irregularly ingrowing rete pegs. One case of inverted papilloma showed the typical ingrowth of basal layer cells deeply into underlying connective tissue, resulted in the formation of multiple fissures on the surface area. The present study also revealed the predominant nuclear vacuolization suspicious for viral infection in papillary papilloma, and also heavy smoking history in the verrucous papilloma. On the other hand, as the epithelium of inverted papilloma ingrew into the underlying deep connective tissue up to the muscular layer, it is supposed that the inverted papilloma has a potential of basal layer proliferation strong enough to invade the protecting barrier of underlying connective tissue, or that the underlying connective tissue was too weak to prevent the ingrowth of basal layer epithelium. Taken together, because the OSCPs show heterogenous origins and variable pathological prognosis, it is suggested that the OSCPs should be differentially diagnosed at least into three types, i.e., papillary papilloma, verrucous papilloma, and inverted papilloma.

Differential Diagnosis of Papillary Papilloma, Verrucous Papilloma, and Inverted Papilloma from Oral Squamous Cell Papillomas

이상신,김현주,박성연,김연숙,이석근 대한구강악안면병리학회 2012 대한구강악안면병리학회지 Vol.36 No.2

Oral squamous cell papillomas(OSCPs) showed various features in their etiology, histology, and prognosis. Therefore, it is necessary to diagnose differentially according to their pathological examinations. In the present study total 14 cases of OSCPs were evaluated and characterized to be three types of OSCPs, i.e., papillary papilloma, verrucous papilloma, and inverted papilloma. The present study demonstrated that among 14 cases of OSCPs papillary papilloma (n=6) showed the typical papillary projection of squamous epithelium with severe acanthosis of upper spinous layer cells, accompanying frequent nuclear vacuolization. And verrucous papilloma (n=7) showed the diffuse acanthosis of whole spinous layer cells with severe basal hyperplasia, resulted in the thick squamous epithelium exhibiting verrucous surface and irregularly ingrowing rete pegs. One case of inverted papilloma showed the typical ingrowth of basal layer cells deeply into underlying connective tissue, resulted in the formation of multiple fissures on the surface area. The present study also revealed the predominant nuclear vacuolization suspicious for viral infection in papillary papilloma, and also heavy smoking history in the verrucous papilloma. On the other hand, as the epithelium of inverted papilloma ingrew into the underlying deep connective tissue up to the muscular layer, it is supposed that the inverted papilloma has a potential of basal layer proliferation strong enough to invade the protecting barrier of underlying connective tissue, or that the underlying connective tissue was too weak to prevent the ingrowth of basal layer epithelium. Taken together, because the OSCPs show heterogenous origins and variable pathological prognosis, it is suggested that the OSCPs should be differentially diagnosed at least into three types, i.e., papillary papilloma, verrucous papilloma, and inverted papilloma.

상기도의 유두종에 대한 임상적 고찰

오경균,장경만,이국행,심윤상,추무진 釜算慶南耳鼻咽喉科學會 1993 임상이비인후과 Vol.4 No.2

유방의 양성 및 악성 유두상 종양에서 Cyclin D1, MIB-1, p53 및 c-erbB-2 발현

김태현,윤기영 고신대학교 의학부 2001 高神大學校 醫學部 論文集 Vol.16 No.1

Background Histological differentiation between papillary carcinoma from benign papilloma of the breast is difficult in some instances, and papillomas can undergo malignant transformation. The author investigated the expression of cyclin D1, MIB-1 (Ki-67), p53, and c-erbB-2-to assess the diagnostic value of these markers in distinguishing papillary carcinoma from papilloma of the breast. Methods Immunohistochemical stains for cyclin D1. MIB-1, p53, and c-erbB-2 were performed in 30 cases of papilloma (20cases of single lesion and 10 cases of multiple lesion) and 8 cases of papillary carcinoma of the breast. Results Labelling index of cyclin D1 was significantly higher in 8 papillary carcinomas (36.7±27.0) than in 30 papillomas (3.5±4.2) (P=0.001). MIB-1 labelling index was 4.9±5.9 in 8 papillary carcinomas and 0.4±1.4 in 30 papillomas, and there was statistically significant difference (P=0.0225). P53 protein expression rate was significantly higher in 8 papillary carcinomas (87.5%) than in 30 papillomas (30.3%) (P=0.00541). There were no difference of cyclin D1, MIB-1, and p53 expression between solitary intraductal papillomas and multiple papillomas. In all benign and malignant papillary lesions, c-erb-B2 expression was not seen. Conclusion These results suggest that cyclin D1, MIB-1, and p53 might be useful markers for distinguishing papillary carcinomas from papillomas of the breast.

비·부비동 반전동 유두종에서 인유두종 바이러스 및 Epstein-Barr바이러스의 검출과 p53단백 및 Proliferating Cell Nuclear Antigen의 발현

조진희,서병도,전범조,장한성,양명재,윤희로,노우영 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.8

Gastric Squamous Papilloma in a 52-Year-Old Female Patient

Hyung Ha Jang,김형욱,Su Jin Kim,최철웅,박수범,Byeong Jun Song,신동훈,강대환 대한소화기내시경학회 2014 Clinical Endoscopy Vol.47 No.6

A papilloma is a benign epithelial lesion characterized by finger-like projections of tissue lined by an overgrowth of squamous cells and a core of connective tissue. We report a case of squamous papilloma on the cardia in a 52-year-old asymptomatic female. Endoscopy showed a 1-cm sized is polyp with hyperemic change originating from the cardia adjacent to the esophagogastric junction, the biopsy of which suggested a diagnosis of squamous papilloma. Endoscopic mucosal resection was performed to obtain a definite diagnosis and the polyp was completely removed. The histological result was compatible with squamous papilloma, and its surrounding tissues showed foveolar epithelium, which suggested a stomach origin. This is the first report of endoscopic resection of a gastric squamous papilloma. Squamous papilloma should be considered in the differential diagnosis of a gastric polyp, especially one in the cardia. As the prognostic value of a squamous papilloma is not well known, we recommend endoscopic resection to treat a gastric squamous papilloma, when possible.

Glandular papilloma of the lung with malignant transformation

( Woo Jung Sung ) 영남대학교 의과대학 2017 Yeungnam University Journal of Medicine Vol.34 No.2

Glandular papilloma of the lung is one of three histologic types of solitary endobronchial papillomas. It is known as an uncommon benign neoplasm. No malignant glandular papillomas have been reported. Herein, the first case of granular papilloma with malignant transformation is reported. A 74-year-old man with huge right lung mass extended upper and lower lobe was admitted to the hospital complaining of progressive cough and dyspnea. An open lung biopsy was performed. Microscopically, the tumor showed papillary growth pattern with thick fibrovascular cores. The stroma of the fibrovascular cores shown the infiltration of lymphoplasmacytic cells and proliferation of capillaries. The epithelial cells surrounding the papillary fronds were cilliated columnar cells with focal cellar atypia, and frequent mitoses. Suspicious pleural invasion foci were identified. The Ki-67 labeling index was about 24.3% and p53 labeling index was about 31.7%. Glandular papilloma is known as a benign neoplasm, which is lack of atypia and mitosis. In present case, there were several indications of malignant transformation, such as cellular atypia, frequent mitosis, architectural distortion, and pleural invasion. Pathologists must be aware that glandular papilloma can have a changes of malignant transformation. Further studies about disease behavior and molecular characteristics are needed.

비강 및 비인두에 발생한 도립유두종과 편평상피암종의 p53단백 및 세포증식능에 관한 연구

한주호,윤기중,이재규,신대균,박근호,조향정,문형배 圓光大學校 醫科學硏究所 1996 圓光醫科學 Vol.12 No.2

The inverted papilloma and squamous cell carcinoma are common neoplasia in the sinonasal cavity and nasopharynx, but the incidence of these tumors are very low and the study on the oncogenesis or biological activity of the tumor cells are not well known. This study was designed to evaluate the oncogenic roles of the p53 gene and the proliferative activity of the tumor cells in the inflammatory polyp, inverted papilloma and squamous cell carcinoma. The experiment was carried by the immunohistochemical stains about the p53 protein, PCNA and Ki-67, histochemical stain about the AgNORs. and flow cytometric analysis about the DNA ploidy using the formalin fixed paraffin embedded tissues. The frequency of the expression of p53 protein was 0%(0/16 cases) in the inflammatory polyps, 18.8% (3/16 cases) in the inverted papillomas, and 87.5%(14/16 cases) in the squamous cell carcinomas. The labelling index(%) of the PCNA and Ki-67 was 5.3% and 3.3% in the inflammatoy polyps, 29.6% and 25.2% in the inverted papillomas, and 51.9% and 36.8% in the squamous cell carcinoma. The expression of the PCNA and Ki-67 was distributed in the periphery of the tumor islands of the inverted papilloma and was distributed in the both of center and periphery of the tumor islands of the squamous cell carcinoma. The number of nuclear AgNORs was increased in the order of inflammatory polyps (0.96), inverted papillomas(1.34) and squamous cell carcinoma(2.09). The frequency of the DNA aneuploidy was 0%(0/16 cases) in the inflammatory polyps, 18.8%(3/16 cases) in the inverted papillomas. and 12.5%(2/16 cases) in the squamous cell carcinomas. Above results indicates that the changes of the p53 gene and proliferative activity of the tumor cells are involved on the oncogenesis and the biological activity of the inverted papilloma or squamous cell carcinoma in the nasopharynx and sinonasa cavity.