토끼에서 유산 발효유제품 급여에 의한 Escherichia coli O157:H7 및 Salmonella typhimurium의 증균억제효과

신광순,김용환,손원근,석주명,김상현 한국식품위생안전성학회 1997 한국식품위생안전성학회지 Vol.12 No.3

The growth inhibition effect of Orally administrated yogurt ACE and Metchnikoffupon E. coli O157:H7 and S. typhimurium inoculated into gastric lumen of rabbits was in vestigated. The rabbits challenged with each 1 $m\ell$ of suspension containing 108 CFU/$m\ell$ of the pathogens were divided into 4 groups by the interval of yogurt administration: A group; preadministrated 7 days before inoculation of the pathogens and fed daily; B group; administrated daily after inocjlation of the pathogens, C group; administrated every 3 days after inoculation of the pathogens; Control group, not fed after inoculation of the pathogens. Each 3 $m\ell$ of yogurt containing 109 CFU/$m\ell$ was orally administrated into rabbits. All yogurt administrated groups (A, B, c) chowed growth ingibition effect on E. coli O157:H7 in one day after inoculation of the pathogen by the level of 0.8~1.0 log CFU/g, compared with the result differences between the control group and the yogurt administrated groups. In the control group after 5 days of inoculation, the number of colonized pathogens was 105~106 CFU/g, whereas 103~104 CFU/g was detected in the yogurt administrated groups. After 10 days of inoculation, the viable pathogen number per gram (g) of the rabbit feces was 103 CFU/g in the control group, whereas the number below 101 CFU/g was detected in the group A, and 102 CFU/g in the control group, B and C. The growth inhibition effect of yogurt administration on E. coli O157:H7 was highly increased in the order of A, B, and C group. The same effect on S. typhimurium was observed at the level of 2 log CFU/g in the Metchnikoff yogurt administrated groups, compared with the control group result in one day after inoculation of the pathogen. In 7 days after inoculation of the pathogen, the viable number was increasingly decreased, and finally after 15 days no viable cell of S. typhimurium was discharged into the fecal samples in the group A, and the mean level of 10* CFU/g was detected in the group B, but there was no growth inhibition effect in the group C. The growth inhibition effect on S. typhimurium was observed at the same level of viable cell number between the yogurt ACE administrated groups and the control group in 5 days after inoculation. But, after 10 days of inoclation the viable cell number was started to decrease, and the viable cell of S. typhimurium was not discharged from rabbit intestinal contents after 15 days of inoculation in the yogurt ACE administrated groups. In such a case that yogurt was administrated in order to prevent the pathogens, pre-administration on a daily basis one week before inoculation of the pathogens exerted considerable effect in growth inhibition. In comparison with two kinds of yogurt tested in this study, the growth inhibition effect on two kinds of pathogens was observed more highly in the Metchnikoff administated group than the ACE administrated group.

COX 억제제에 의해 유도되는 구강편평세포암종 세포주의 성장 억제 효과

박광진,한세진,이재훈,Park, Gwang-Jin,Han, Se-Jin,Lee, Jae-Hoon 대한악안면성형재건외과학회 2008 Maxillofacial Plastic Reconstructive Surgery Vol.30 No.4

The objectives of this study was to explore the growth pattern of the oral squamous cell carcinoma when overexpressed COX was inhibited, explore the pathway that COX inhibitors suppressed the proliferation of cancer cells, and then hereafter investigate the potential of COX as chemopreventive target for oral squamous cell carcinoma. For confirming the COX-dependent effect and mechanisms on growth of the oral cancer cells, we treated the nonselective NSAID, Mefenamic acid and COX-2 selective inhibitor, Celecoxib in HN4 cell line. And then the cell line was evaluated with MTT assay and growth curve, the production of PGE2, total RNA extraction and RT-PCR analysis, and TEM The results were obtained as follows: 1. After administration of medication, in the result of MTT assay, Celecoxib inoculated group inhibit the cell growth rather than Mefenamic acid inoculated group. 2. The growth curve of cell line showed as time passes by there was a dramatic cell growth in the control group, and gradual growth inhibition was found in medication inoculated group and, in Celecoxib inoculated group there was more inhibition of cell growth. 3. After the administration of medication, Celecoxib tend to inhibit the synthesis of PGE2 more than Mefenamic acid. Mefenamic acid inhibit the synthesis of PGE2 more as the concentration gets high, but Celecoxib inhibited the synthesis of PGE2 even in low concentration. 4. After the administration of medication, the revelation of COX mRNA in cell line, there was a 50% decrease in COX-1, 60% decrease in COX-2 as in $50{\mu}M$ Mefenamic acid, and in Celecoxib $50{\mu}M$ there was not much difference in COX-1 and 90% decrease in COX-2 was found. 5. HN4 cell line showed broken nucleus and tangled cytoskeleton bundles in cytoplasm which meant apoptotic features after the treatment of Celecoxib in TEM view. Depending on the above results, we estimate that the inhibition of the expression of COX-2 cause the growth suppression of the oral squamous cell carcinoma, and it get achieved through pathway of reduced PGE2 production and increased apoptosis. In addition to, because COX-2 selective inhibitor specifically act to COX-2, it is considered that COX-2 selective inhibitor has the adequate potential as chemopreventive agent for oral squamous cell carcinoma.

Benzaldehyde as a new class plant growth regulator on Brassica campestris

( Geun Hyoung Choi ),( Jin Ho Ro ),( Byoung Jun Park ),( Deuk Yeong Lee ),( Mi Sun Cheong ),( Dong Yeol Lee ),( Woo Duck Seo ),( Jin Hyo Kim ) 한국응용생명화학회 2016 Journal of Applied Biological Chemistry (J. Appl. Vol.59 No.2

Plant growth regulator is an essential pesticide to date while the available active ingredient is not well understood unlike fungicide, insecticide and herbicide. This study was aimed to evaluate a new chemical class of plant growth regulator, and the total of 92 benzene derivatives were screened for their germination and early stage of the root growth regulation on Brassica campestris. Thirty benzaldehydes, nine acids, one amide, and one ester showed potent root growth inhibitory activity (>70 % inhibition) while only salicylaldehyde showed potent germination inhibition (IC50 = 81.2 mg/L) suggesting that benzaldehyde was a key module candidate for the growth inhibition. Benzaldehydes were further evaluated for root growth inhibition. 2,3-Dihydroxybenzaldehyde and salicylaldehyde showed IC50 values of 8.0 and 83.9 mg/L, respectively. On the other hand, salicylaldehyde, and 2,4,5- trihydroxybenzaldehyde were found to have root growth promotion effects less than 10 mg/L. This result suggests that the benzaldehyde is a new class candidate for plant growth regulator.

Effect of the D-glucose analog, D-allose, on the growth of Arabidopsis roots

Kato-Noguchi, Hisashi,Takaoka, Takuya,Okada, Kozue The Korean Society of Weed Science and The Turfgra 2011 Weed Biology and Management Vol.11 No.1

Although D-glucose increased the root growth of Arabidopsis seedlings, D-allose (a d-glucose epimer at the third carbon atom) inhibited the root growth at concentrations >0.1 $mmol\;L^{-1}$ and the inhibition increased with increasing D-allose concentrations. Allitol (a reduction product of D-allose) did not show any significant effect on the growth. The addition of D-glucose into the growth medium of Arabidopsis reversed the D-allose-induced growth inhibition, which suggests that the inhibition is not caused by the toxicity of the accumulation of D-allose and/or its metabolites in the seedlings. D-Allose is phosphorylated by hexokinase, using ATP and phosphate, to allose-6-phosphate, with no known capacity for further metabolism. The addition of phosphate into the growth medium did not affect the D-allose-induced growth inhibition and D-allose did not reduce the ATP level in the roots. These results suggest that the inhibition is not due to phosphate starvation and ATP depletion. D-Mannoheptulose, a specific competitive inhibitor of hexokinase, defeated the D-allose-induced growth inhibition. Hexokinase is known to have a sugar-sensing function and possibly triggers a signal cascade, resulting in the change of several gene expressions. Therefore, the phosphorylation of D-allose by hexokinase might trigger a signal cascade, resulting in the inhibition of Arabidopsis root growth. This is probably a useful model system for studies of the hexokinase-mediated sugar-sensing function and for developing new types of weed-control agents.

꽃송이버섯 추출물이 제모된 C57BL/6 마우스의 모발성장 및 억제에 미치는 영향

류은미,신현재,나명순 한국피부과학연구원 2010 대한피부미용학회지 Vol.8 No.4

Sparassis crispa is an edible and medicinal mushroom, which commonly called the cauliflower mushroom. S. crispa is known to be a good source of food and nutraceuticals due to their rich flavors and β-glucan contents as well as a good source of antiviral and anticancer compounds. However, there have been no report on the effect on hair growth stimulation and/or growth inhibition. C57BL/6 mice the most widely used lab mouse strain were used in this animal study due to their availability and the similar hair growth cycle to human hair. In this study, hair-removed five-year-old C57BL/6 mice were used to examine the effect of S. crispa extracts(70% ethanol) on hair growth stimulation and inhibition of the mice. The extracts and control compounds were topically treated once a day for 4 weeks. The hair growth was photographically and histologically examined during the total period of 4 weeks. Hair growth of the test group was a little inhibited compared to positive (3% minoxidil) and negative control groups (water and ethanol treated groups), respectively. The whole data showed little improvement of hair growth effect of S. crispa extracts but with no real statistical significance. These results suggest that S. crispa extract has little hair growth promoting activity and more works should be done to elucidate the inhibition mechanism. 본 연구는 최근 면역 및 항암활성 등 혈류 개선과 관련된 약리적 효과로 주목받고 있는 꽃송이버섯 추출물이 발모․양모 및 제모에 어떠한 영향을 미치는지 알아보고자 실험동물을 통하여 실험적 연구를 수행하였다. 실험은 실험군의 꽃송이버섯 추출물과 대조군의 무시료, 에탄올 70%, 버섯추출물, 미녹시딜 3%로 나누어 실험동물 C57BL/6 mouse(female)에 처리하였다. 실험시료에 따라 실험군과 대조군으로 나누어 총 5군을 실험에 이용하였다. 7주령 mouse의 등판을 제모한 뒤 4주(28일)동안 시료에 따른 양모효과를 육안적 관찰하였고, 경피 적출 후 조직학적인 검사를 수행하여 다음과 같은 결과를 얻었다. 1. 털의 성장정도를 알아보기 위한 mouse 외형의 육안적 관찰결과, 실험군인 꽃송이버섯 추출물이 대조군의 버섯추출물, 에탄올, 무시료, 미녹시딜 보다 모발의 성장이 억제됨이 관찰되었다. 2. 4주(28일)째 mouse를 경추 도살하여 얻은 피부조직의 모낭형태를 광학현미경으로 관찰한 결과, 실험군인 꽃송이버섯 추출물에서 대조군에 비해 모낭조직의 길이가 짧고 피부 위쪽에 위치하고 있었으며, 모낭내의 모근이 짧게 성장하여 피부의 표면으로 자라는 모낭 형태를 하고 있었다. 결론적으로 꽃송이버섯 추출물을 C57BL/6 mouse(female)의 등부위에 처리한 결과, 육안적 외형분석 및 조직학적 분석에서 실험군이 대조군보다 모발 성장이 억제되는 것으로 나타남에 따라 실험군인 꽃송이버섯 추출물은 모발 성장억제 효과가 있는 것으로 사료된다.

Use of Seawater and Pesticides (Geunsami and Pullmagi Gold) for Suppressing Growth of Spartina anglica and Their Effects on Soil Stability

이은숙,변재영,Maynanda Brigita Chrysta,Okenkwu Nnaemeka Nicholas,이세용,최원식 사단법인 미래융합기술연구학회 2020 아시아태평양융합연구교류논문지 Vol.6 No.2

Common cordgrass (Spartina anglica) is a harmful marine organism that has proved to be a serious invasive species causing extensive damage to natural saltmarsh ecosystems in many areas. In order to reduce the damage that may occur from spreading these plants, it takes the best steps to destroy them. In this study, an experiment of Spartina anglica growth suppression was carried out. The media used to eradicate these weeds were seawater concentrate and pesticides (Pullmagi gold and Geunsami). In testing Spartina anglica and clover growth inhibition, samples were planted in mudflats A, B, and C. In mudflats (A) sprayed seawater concentrate using 5L stock solution, (B) sprayed 5L liquid herbicide and 25ml pesticides, and (C) sprayed liquid herbicide 5L and 50ml pesticides. As a result, after 5 days of spraying, the growth inhibition of the sliding plant showed that variation (A) with seawater concentrate showed rapid growth inhibition, while for variation (B) and (C) that using herbicides did not show growth inhibition. The change in growth inhibition on variation A was remarkable. The seawater concentrate showed growth inhibition of whole leaves after 1 day. The results of the five heavy metals analysis of soils were also not detected and the growth inhibition and safety of plants after the experiments are analyzed in the sludge and seagrass of sea soils.

14-3-3 Sigma and 14-3-3 Zeta Plays an Opposite Role in Cell Growth Inhibition Mediated by Transforming Growth Factor-Beta 1

Hong, Hye-Young,Jeon, Woo-Kwang,Bae, Eun-Jin,Kim, Shin-Tae,Lee, Ho-Jae,Kim, Seong-Jin,Kim, Byung-Chul Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.29 No.3

The expression of 14-3-3 proteins is dysregulated in various types of cancer. This study was undertaken to investigate the effects of 14-3-3 ${\zeta}$ and 14-3-3 ${\sigma}$ on cell growth inhibition mediated by transforming growth factor-beta 1 (TGF-${\beta}$1). Mouse mammary epithelial cells (Eph4) that are transformed with oncogenic c-H-Ras (EpRas) and no longer sensitive to TGF-${\beta}$1-mediated growth inhibition displayed increased expression of 14-3-3 ${\zeta}$ and decreased expression of 14-3-3 ${\sigma}$ compared with parental Eph4 cells. Using small interfering RNA-mediated knockdown and overexpression of 14-3-3 ${\sigma}$ or 14-3-3 ${\zeta}$, we showed that 14-3-3 ${\sigma}$ is required for TGF-${\beta}$1-mediated growth inhibition whereas 14-3-3 ${\zeta}$ negatively modulates this growth inhibitory response. Notably, overexpression of 14-3-3 ${\zeta}$ increased the level of Smad3 protein that is phosphorylated at linker regions and cannot mediate the TGF-${\beta}$1 growth inhibitory response. Consistent with this finding, mutation of the 14-3-3 ${\zeta}$ phosphorylation sites in Smad3 markedly reduced the 14-3-3 ${\zeta}$-mediated inhibition of TGF-${\beta}$1-induced p15 promoter-reporter activity and cell cycle arrest, suggesting that these residues are critical targets of 14-3-3 ${\zeta}$ in the suppression of TGF-${\beta}$1-mediated growth. Taken together, our findings indicate that dysregulation of 14-3-3 ${\sigma}$ or 14-3-3 ${\zeta}$ contributes to TGF-${\beta}$1 resistance in cancer cells.

14-3-3 Sigma and 14-3-3 Zeta Plays an Opposite Role in Cell Growth Inhibition Mediated by Transforming Growth Factor-Beta 1

Hye-Young Hong,전우광,배은진,김신태,이호재,김성진,김병철 한국분자세포생물학회 2010 Molecules and cells Vol.29 No.3

The expression of 14-3-3 proteins is dysregulated in various types of cancer. This study was undertaken to investigate the effects of 14-3-3 ζ and 14-3-3 σ on cell growth inhibition mediated by transforming growth factor-beta 1(TGF-β1). Mouse mammary epithelial cells (Eph4) that are transformed with oncogenic c-H-Ras (EpRas) and no longer sensitive to TGF-β1-mediated growth inhibition displayed increased expression of 14-3-3 ζ and decreased expression of 14-3-3 σ compared with parental Eph4 cells. Using small interfering RNA-mediated knockdown and overexpression of 14-3-3 σ or 14-3-3 ζ, we showed that 14-3-3 σ is required for TGF-β1-mediated growth inhibition whereas 14-3-3 ζ negatively modulates this growth inhibitory response. Notably, overexpression of 14-3-3 ζ increased the level of Smad3 protein that is phosphorylated at linker regions and cannot mediate the TGF-β1 growth inhibitory response. Consistent with this finding, mutation of the 14-3-3 ζ phosphorylation sites in Smad3 markedly reduced the 14-3-3 ζ-mediated inhibition of TGF-β1-induced p15 promoter-reporter activity and cell cycle arrest,suggesting that these residues are critical targets of 14-3-3 ζ in the suppression of TGF-β1-mediated growth. Taken together, our findings indicate that dysregulation of 14-3-3 σ or 14-3-3 ζ contributes to TGF-β1 resistance in cancer cells.

Growth-inhibiting Effects of Brazilian and Oriental Medicinal Plants on Human Intestinal Bacteria

Kim, Moo-Key,Lee, Sung-Eun,Lee, Hoi-Seon 한국응용생명화학회 2000 Journal of Applied Biological Chemistry (J. Appl. Vol.43 No.1

Methanol extracts of 27 Brazilian plant samples and 10 oriental medicinal plant samples (27 families), using spectrophotometric and paper disc agar diffusion methods under anaerobic conditions, were tested in vitro for their growth-inhibiting activities against Bifidobacterium longum, Bifidobacterium bifidum, Bifidobacterium adolescentis, Clostridium perfringens, and Bacteroides fragilis. The responses varied with bacterial strains, plant species, and tissues sampled. In a test with B. longum and B. bifidum(20 mg/disc), extracts of Acanthopanax sessilifolinus stem bark and Ampelozizyphus amazonicus leaves strongly inhibited the growth of B. longum, whereas other plant samples did not inhibit any intestinal bacteria tested. At 5 mg/disc, adding extracts of Aralia eleta, Euterpe oleracea, and Syzygium guineense to the media strongly inhibited the growth of C. perfringens and B. fragilis without growth inhibition of B. adolescentis, B. longum, and B. bifidum. Extracts of Jacaranda mimosifolia and Ulmus paraifolia significantly inhibited the growth of C. perfringens and B. fragilis as well as B. adolescentis. These results may be indications of at least one of the pharmacological actions of the five Brazilian plants but not oriental medicinal plants tested.

Growth Responses of Seven Intestinal Bacteria Against Phellodendron amurense Root-Derived Materials

( Kim Min Jeong ),( Sang Hyun Lee ),( Jang Hee Cho ),( Moo Key Kim ),( Hoi Seon Lee ) 한국미생물생명공학회 2003 Journal of microbiology and biotechnology Vol.13 No.4