Relationship Between Color Characteristic and Reflectance Index by Ground-based Remote Sensor for Tobacco Leaves

Soon-Dal Hong(홍순달),Seong-Soo Kang(강성수),Sang-Ho Jeon(전상호),Hyun-Cheol Jeong(정현철) 한국토양비료학회 2009 한국토양비료학회지 Vol.42 No.4

지상원격탐사 센서를 이용한 황색종의 적숙엽 판단기준을 평가하기 위하여 성숙기에 중엽과 상엽부위에 대하여 수확엽의 녹색도를 5개 수준으로 구분하여 여러 가지 센서지표들의 상호관계를 평가하였다. 건조엽의 색 특성은 색차계(Colorimeter, CR-300)를 이용하여 L, a, b값을 측정하였고 건조엽의 청색도는 육안관찰에 의한 분포정도를 수치화하여 비교 검토하였다. 수확엽의 녹색도에 따른 반사율은 550nm와 675nm에서 녹색도가 증가할수록 감소되는 특성을 보였다. 건조엽의 반사율은 미숙된 엽이 건조된 후 녹색이 잔류되었기 때문에 미숙엽의 675nm 반사율이 더 낮아졌다. 그 결과 동일한 엽위에서 생엽과 건조엽의 반사율지표는 직선적인 정의 상관을 보였다. 또한 건조엽의 청색도는 모든 센서지표들과 유의성 있는 정의 상관을 보였고 명도를 나타내는 색차계 L값은 센서지표들과 유의성 있는 부의 상관을 보였다. 따라서 건조엽의 청색도로 평가된 센서 종류별 적숙엽의 기준은 엽록소 측정치 SPAD 값은 22 이하, 엽록소 측정치 CM-1000 값은 135 이하, 그리고 원격탐사센서 Crop Circle의 gNDVI 값은 0.43 이하로 평가되었다. To determine the critical level for optimum maturity of flue-cured tobacco leaves (KF118) at the stalk position from cutter to tips, the reflectance index using ground-based remote sensors and chlorophyll meter were investigated. The sensors estimated were Crop CircleTM (Holland Scientific), Green SeekerTM (Ntech Industries), Spectroradiometer (LICOR, LI-1800), Chlorophyll meter (SPAD502, Minolta), and Field ScoutTM Chlorophyll meter (CM-1000, Spectrum). The L, a, b values and greenness for flue-cured leaf were measured and estimated for correlation with sensor"s measurement of harvested leaf. On a reflectance curve of 340nm~1100 nm, the reflectance peaks on 550nm and 675 nm for the harvested leaf were lowered as change from light green to darker green. Darker green leaf harvested produced darker flue-cured leaf. The reflectance at 675 nm for flue-cured leaf decreased as greenness increased in the harvested leaf. This result means that the red edge band of 675 nm wavelength is related to the absorbance of chlorophyll for photosynthesis. The greenness of flue-cured leaf showed significantly positive correlation with the entire reflectance indexes for harvested leaf while the L value by colorimeter showed negative correlation with greenness of cured leaf. The critical level for optimum maturity of harvested leaf were less than 22, 135, and 0.43 for SPAD reading, CM-1000 reading, and gNDVI by Crop CircleTM, respectively. Consequently, ground-based remote sensing providing a non-destructive real-time assessment of plant greenness could be a useful tool in the selection of optimum maturity of flue-cured tobacco leaves in relation to high quality of flue-cured tobacco

근적외 분광분석법을 이용한 한국산과 미국산 잎담배의 판별분석

장기철,김용옥,이경구 한국연초학회 1998 한국연초학회지 Vol.20 No.2

Discriminant analysis using near infrared spectra derived from Korean Flue-cured(KF) and American Flue-cured(AF), and also Korean Burley(KB) and American Burley(AB) tobacco was done to classify flue-cured and burley tobacco as either grown in Korea or grown in the USA. Samples were scanned in the wavelength of 400 ~ 2500 nm by near infrared analyzer(NIRSystem Co., model 6500). The discrimination equations for flue-cured and burley tobacco were developed using partial least square 2 method in Infrasoft International NIRS 3 software package. KF samples used for the development of the discrimination equations were higher contents of total sugar, crude ash and chlorine, and higher value of leaf density and brightness, but lower contents of nicotine, total nitrogen and ether extracts, and higher value of redness than those of AF samples. KB samples were higher contents of nicotine, crude ash and chlorine, but lower contents of ether extracts and value of brightness than those of AB samples. On 3 dimensional graph drawn with 3 principal component scores calculated with 3 principal component from KF and KB sample spectra, KF sample spectra were significantly different from AF, and also KB sample spectra were significantly different from AB. The discrimination equations of flue-cured and burley were developed with 3 principal component, respectively. The discrimination equations for flue-cured and burley had a standard error of 0.03 and 0.04, and a R2 of 0.88 and 0.84, respectively. The tobacco samples used for the development of discrimination equation were perfectly classified as KF and AF by flue-cured discrimination equation, and also perfectly classified KB and AB by burley discrimination equation, respectively. The correct classification rates of KF and AF samples not used for the development of discrimination equations were 9S % (828 out of 869 samples) and 98 % (98 out of 100 samples) by flue-cured discrimination equations, and KB and AB samples were 94%(345 out of 368 samples) and 100%(42 out of 42 samples) by burley discrimination equations, respectively.

황색종 건조시 황변엽의 탈수건조 조건에 따른 건조엽의 품질

석영선,이철희,C. W. Suggs 한국연초학회 2003 한국연초학회지 Vol.25 No.1

This study was carried out to investigate the changes of dehydration process and quality of cured leaves with crushed midribs and cut of the yellowed leaves during drying stage of flue-cured tobacco. The crushed midribs of yellowed leaf dried out before raising the highest temperature of midrib drying stage and reduced the curing time by about 20 hours. However, the cut of yellowed leaf was not affected the curing time and dehydration process during drying stage of flue-curing due to a problem with the cut leaves packing excessively in the curing container. The cured weight yield was increased with crushed midrib and cut leaves, and nicotine and sugar contents of cured leaves and quality in terms of dollars per kilogram were decreased with crushed midrib and cut leaves.

토양환경, 재배방법 및 기상요인이 황색종 잎담배 화학성분에 미치는 영향

정기택,김상범,조수헌,복진영,이종률,Jeong Kee-Taeg,Kim Sang-Beom,Cho Soo-Heon,Bock Jin-Young,Lee Joung-Ryoul 한국연초학회 2006 한국연초학회지 Vol.28 No.1

This study was conducted to investigate the effect of soil, cultural practices and climatic conditions on some chemical constituents of flue-cured tobacco. Increasing the nicotine and total nitrogen contents may be useful to reduce the total sugar content of cured leaves in flue-cured tobacco. Delaying the transplanting date and increasing the soil nitrogen($N0_3-N$ and $N0_4-N$) content for 30 days after transplanting by fertilizing are desirable so as to increase the nicotine and total nitrogen contents of cured leaves. Those treatments will delay the ripeness, and elongate the duration of cultivation(day from transplanting to harvesting), and increase the fresh leaf weight. Moderate rainfall in April and May, lower relative humidity in June, and higher mean daily air temperature in June and July seem to be necessary for good leaf of flue-cured tobacco in Korea.

세계 5개국에서 생산된 황색종 잎담배의 주요 향기성분 비교

이태호,신경은,이재현,이은석,한병석,임흥빈,Lee Tae-Ho,Shin Kyung-Eun,Lee Jae-Hyun,Lee Eun-Sung,Han Byung-Seuk,Lim Heung-Bin 한국연초학회 2004 한국연초학회지 Vol.26 No.2

The present study was carried out to compare the composition of major essential oil components in the flue-cured tobacco produced by Korea, America, China, Brazil and Zimbabwe. Above 100 essential oil components were separated by GC and major 18 components of them, known to be important contributors to flue-cured tobacco flavor and smoke taste, were identified by GC/MS. Neophytadiene was major in quantities in the oils mostly and its composition in flue-cured tobacco produced by Korea, America, Zimbabwe, Brazil, and Unnam and Yenji region of China was 26.82\%,\;25.17\%,\;26.50\%,\;16.92\%,\;and\;18.75\%\;and\;14.87\%$, respectively. Megastigmatrienone, one of the major tobacco carotenoid degradation products was contained above $10\%$ in the oils of flue-cured tobacco produced by Korea and America, but, it was comprised about $5.66\%$ to 8.00 in Brazil, Zimbabwe, and Unnam and Yenji region of China. Damascenone is important to the aroma of tobacco as a crotenoid degradation products. Its amount in the oils was $3.31\%\;in\;Brazil,\;3.13\%\;in\;America,\;2.57\%\;in\;Zimbabwe,\;2.54%$ in Yenji of China, $2.00\%$ in Korea and $1.85\%$ in Unnam of China. These results can furnish the basic information capable of evaluating the quality value of flue-cured tobacco produced by various nations.

황색종 NC82와 KFl14의 건조단계별 정유성분의 변화

홍열,임흥빈,석영선,신주식,김종열,나도영,이학수,Hong, Yeol,Lim, Heung-Bin,Seok, Young-Sun,Shin, Ju-Sik,Kim, Jong-Yeol,Ra, Do-Young,Lee, Hak-Su 한국연초학회 2001 한국연초학회지 Vol.23 No.2

Essential oil in tobacco leaves influences the taste and aroma of cigarette smoke and is important to tobacco quality. This study was conducted to investigate the change in the level of essential oil components during flue-curing process of two flue-cured tobaccos, NC82 and KEl14. Flue-curing process was divided by six steps; harvest stage, the end of yellowing stage, the middle of color fixing stage, the end of color fixing stage, the middle of midrib drying stage, full-cured stage. NC82 in each stage contained 0.28%, 0.30%, 0.35%, 0.36%, 0.40% and 0.42% essential oil, respectively, and KF114 were 0.29%, 0.31%, 0.34%, 0.36%, 0.39% and 0.41%, respectively. Almost all hydrocarbons on the basis of relative peak area were gradually increased in two varieties with curing, neophytadiene content in them was highest at the full-cured stage. Most of alcohols and esters with curing showed a declining trend, but benzyl alcohol was increased in two tobaccos. Ketones were largely increased at the midrib drying stage during the curing process, especially, the most largely increasing constituent was $\beta$-damascenone among them. The content of 2-butylterahydrofuran, heterocyclic compounds, was largely increased at tile color fixing stage. There was no considerable difference between NC82 and KFl14 at the GC profile of essential oil and the pattern of each components during flue-curing process.

황색종 잎담배의 전당 함량이 화학성분, 연기 및 관능의 특성에 미치는 영향

정기택,조수헌,복진영,이종률,Jeong, Kee-Taeg,Cho, Soo-Heon,Bock, Jin-Young,Lee, Joung-Ryoul 한국연초학회 2007 한국연초학회지 Vol.29 No.1

This study was conducted to investigate the effect of the total sugar contents on chemical, smoke and sensory properties and to estimate the desirable chemical composition contents from sensory properties in flue-cured leaf tobacco. The leaves used in the study were in 2005 and in $1997{\sim}2006$ crop year. Nicotine and total nitrogen contents, filling value, impact, irritation and bitterness were reduced with increasing the total sugar contents of B2O(leaf) and C2L(cutters), whereas tar and CO contents and puff number were increased. The desirable sugar contents estimated from the best overall tobacco taste of smoke were 25.9% in B2O and 26.9% in C2L. All of regression equations among total sugar/nicotine ratio, total sugar, nicotine and total nitrogen contents were significant($p{\leqq}0.05$). There were no significant difference between the 4 grade(A2O, B2O, C2L, D2L) average and the whole plant(12 grade) average contents in total sugar, nicotine and total nitrogen contents. All of regression equations between the ratio of each grade content to the average content of twelve grades and stalk position[number ; from bottom(1) to top(12)] in total sugar, nicotine and total nitrogen contents were significant($p{\leqq}0.05$). The estimated desirable chemical properties which based on the best overall tobacco taste were 23.2% of total sugar, 2.39% of nicotine, 9.7 of total sugar/nicotine ratio, 2.57% of total nitrogen, 1.08 of total nitrogen/nicotine ratio in average of four grades. With decrease the total sugar content from 32.0% to 24.0%, filling value was enhanced by 14.1% in average of B2O and C2L, also tar and CO contents of smoke were decreased by 13.2% and 10.5%, respectively. These results suggest that the estimated desirable chemical composition contents may be useful to produce good leaf tobacco, and to enhance filling value and to reduce tar and CO contents of smoke in flue-cured leaf tobacco.

황색종 잎담배의 후숙과정 중 유리당, 비휘발성 유기산 및 지방산의 변화

복진영,박윤신,박원종,이종률,Bock, Jin-Young,Park, Yoon-Shin,Park, Won-Jong,Lee, Joung-Ryoul 한국연초학회 2006 한국연초학회지 Vol.28 No.2

This study was carried out to investigate the changes of free sugars, non-volatile organic and fatty acids in flue-cured leaf tobacco during aging. The threshed leaf tobacco(B1O and C1L) produced in 2002 crop year was aged for 21 month in warehouse of Oc-Cheon Leaf Tobacco Processing Factory. The leaf tobacco were sampled at three month intervals for analysis of free sugars, non-volatile organic and fatty acids. The major free sugars of flue-cured were glucose and fructose regardless of tobacco grades. After aging period of 21 months, the contents of glucose and fructose showed a tendency to slightly decrease, and there was no appreciable change in the contents of sucrose in B1O and C1L grades. The major compound of non-volatile organic acid and fatty acid were malic, oxalic and citric, linolenic, linoleic and palmitic acid regardless of tobacco grade. After aging period of 21 months, the contents of malic, malonic, oxalic and fumaric acid showed a tendency to slightly decrease, whereas succinic acid was slightly increased, and maleic acid was not changed in B1O and C1L grades. The decreases in fatty acid contents in B1O and C1L grade tobacco loaves after 21 month aging were 16.5% and 9.8%, respectively. The decreases in linolenic acid contents in two grades were the highest, showing 22.1% and 12.0% reduction after 21 month aging.

황색종 잎담배의 연기응축물중 페놀 화합물에 관한 연구

복진영,백순옥,김상범,안동명,조수헌 한국연초학회 2001 한국연초학회지 Vol.23 No.2

This study was carried out to quantitatively determine phenol compounds in smoke total particulate matter(TPM) to evaluate the qualititive characteristics of flue-cured tobacco varieties(NC82, KFl14 and KFl18). After collecting The TPM by using smoking machine, the concentration of phenol compounds were analyzed by gas chromatography as their trimethylsilyl derivative on a fused capillary column bonded with a 5% PE ME siloxane stationary phase. In all the flue-cured tobacco varieties, the major phenol components were monohydroxy compounds. The order of the highest concentration of total phenol compounds in TPM was NC82, KF114 and KFl18 but the contents of dihydroxy compound in the KFl18 was higher than those of NC82 and KF114.

황색종 잎담배의 후숙과정 중 정유성분의 변화

복진영,박윤신,박원종,이종률,Bock, Jin-Young,Park, Yoon-Shin,Park, Won-Jong,Lee, Joung-Ryoul 한국연초학회 2006 한국연초학회지 Vol.28 No.2

This study was carried out to investigate the changes of essential oils in flue-cured leaf tobacco during aging for 21 months. The threshed leaf tobacco(BlO) produced in 2002 crop year was aged for 21 months in the warehouse of Oc-Cheon Leaf Tobacco Processing Factory. The leaf tobacco were sampled at three month intervals for analysis of volatile compounds. Volatile compounds were identified by GC/MS and comparison of gas chromatographic retention time with those of the authentic standard. The total of 75 compounds from the steam volatile concentrate of the flue-cured leaf tobacco were identified; they were 15 hydrocarbons, 12 alcohols, 3 aldehydes, 18 ketones, 7 esters, 10 acids, 3 phenols, 4 furans, 2 pyrrols and 1 pyridine. The major components of essential oil were neophytadiene, solanone, megastigmatrienone and phytol. After a aging period of 21 months, most of volatile compounds showed a gradual increasing tendency.