Relationship Between Color Characteristic and Reflectance Index by Ground-based Remote Sensor for Tobacco Leaves

Soon-Dal Hong(홍순달),Seong-Soo Kang(강성수),Sang-Ho Jeon(전상호),Hyun-Cheol Jeong(정현철) 한국토양비료학회 2009 한국토양비료학회지 Vol.42 No.4

지상원격탐사 센서를 이용한 황색종의 적숙엽 판단기준을 평가하기 위하여 성숙기에 중엽과 상엽부위에 대하여 수확엽의 녹색도를 5개 수준으로 구분하여 여러 가지 센서지표들의 상호관계를 평가하였다. 건조엽의 색 특성은 색차계(Colorimeter, CR-300)를 이용하여 L, a, b값을 측정하였고 건조엽의 청색도는 육안관찰에 의한 분포정도를 수치화하여 비교 검토하였다. 수확엽의 녹색도에 따른 반사율은 550nm와 675nm에서 녹색도가 증가할수록 감소되는 특성을 보였다. 건조엽의 반사율은 미숙된 엽이 건조된 후 녹색이 잔류되었기 때문에 미숙엽의 675nm 반사율이 더 낮아졌다. 그 결과 동일한 엽위에서 생엽과 건조엽의 반사율지표는 직선적인 정의 상관을 보였다. 또한 건조엽의 청색도는 모든 센서지표들과 유의성 있는 정의 상관을 보였고 명도를 나타내는 색차계 L값은 센서지표들과 유의성 있는 부의 상관을 보였다. 따라서 건조엽의 청색도로 평가된 센서 종류별 적숙엽의 기준은 엽록소 측정치 SPAD 값은 22 이하, 엽록소 측정치 CM-1000 값은 135 이하, 그리고 원격탐사센서 Crop Circle의 gNDVI 값은 0.43 이하로 평가되었다. To determine the critical level for optimum maturity of flue-cured tobacco leaves (KF118) at the stalk position from cutter to tips, the reflectance index using ground-based remote sensors and chlorophyll meter were investigated. The sensors estimated were Crop CircleTM (Holland Scientific), Green SeekerTM (Ntech Industries), Spectroradiometer (LICOR, LI-1800), Chlorophyll meter (SPAD502, Minolta), and Field ScoutTM Chlorophyll meter (CM-1000, Spectrum). The L, a, b values and greenness for flue-cured leaf were measured and estimated for correlation with sensor"s measurement of harvested leaf. On a reflectance curve of 340nm~1100 nm, the reflectance peaks on 550nm and 675 nm for the harvested leaf were lowered as change from light green to darker green. Darker green leaf harvested produced darker flue-cured leaf. The reflectance at 675 nm for flue-cured leaf decreased as greenness increased in the harvested leaf. This result means that the red edge band of 675 nm wavelength is related to the absorbance of chlorophyll for photosynthesis. The greenness of flue-cured leaf showed significantly positive correlation with the entire reflectance indexes for harvested leaf while the L value by colorimeter showed negative correlation with greenness of cured leaf. The critical level for optimum maturity of harvested leaf were less than 22, 135, and 0.43 for SPAD reading, CM-1000 reading, and gNDVI by Crop CircleTM, respectively. Consequently, ground-based remote sensing providing a non-destructive real-time assessment of plant greenness could be a useful tool in the selection of optimum maturity of flue-cured tobacco leaves in relation to high quality of flue-cured tobacco

아로니아 잎의 성숙도에 따른 항산화 물질 조성 및 항산화 능력

양해조(Haejo Yang),박현정(Hyunjeong Park),윤형열(Hyeongyeol Yun),김영준(Young-Jun Kim),신영재(Youngjae Shin) 한국식품과학회 2021 한국식품과학회지 Vol.53 No.2

국내에서 재배한 A. melanocarpa 잎의 성숙도에 따른 이화학적 특성 변화, 항산화 물질 조성 변화 및 항산화 능력과 같은 기능적 특성을 분석하여, 아로니아 잎의 성숙도별 이용 가능성을 확인하였다. A. melanocarpa 3품종의 잎의 폴리페놀, 플라보노이드, 페놀화합물 함량은 성숙이 진행될수록 감소하였고, 항산화물질과 상관관계가 높은 ABTS 라디컬 소거능 및 DPPH 라디컬 소거능 역시 감소하는 경향을 보였다. 현재, 블루베리는 잎과 열매 모두 식품원료로 등록되어 있으나, 아로니아의 경우는 식품의약안전처에서 고시한 식품원료목록 상 열매 부위만 등록되어 있는 실정이다. 아로니아 잎에는 chlorogenic acid와 catechol이 다량 함유되어 있고, 아로니아 잎의 항산화 능력은 기타 베리류와 비교하였을 때, 상당히 높은 수준인 것으로 확인된 바와 같이, 아로니아 잎은 향후 가공 식품 이외에 다양한 분야로의 활용 가능성이 충분할 것으로 판단된다. In this study, the leaves of aronia (Aronia melanocarpa) across different stages of maturity were collected and their chlorophyll content, antioxidant content, and activity were analyzed. The leaves of the selected aronia cultivars (‘Viking’, ‘McKenzie’, and ‘Kingstar K1’) were harvested in June (young-stage leaf) and in August (old-stage leaf). The antioxidant content and activity of all three aronia cultivar leaves were significantly higher in the young-stage leaves than in the old-stage leaves. The main polyphenols in aronia leaves were catechol and chlorogenic acid, which tended to decrease as maturation progressed. As a result, the young-stage aronia leaves contained more abundant flavonoids, phenolic compounds, and polyphenols with higher antioxidant activity than those in the old-stage leaves. Overall, our findings indicate that aronia leaves contain potential bioactive compounds that could be used to develop functional food ingredients.

Improvement of Regeneration Efficiency fron Mature Embryo and Leaf Base Segment in Korean Oat Genotypes

Kyung-Hee Kim,Jung-Hun Moon,Sang-Kyu Lee,Byuang-Moo Lee 韓國作物學會 2004 Korean journal of crop science Vol.49 No.4

Mature embryo and leaf base segment of Korean oat were used as materials in an experiment to check plant regeneration efficiency. MS media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin, and picloram were used for callus induction from mature embryos and leaf base segments. Three mg/l of 2,4-D and 3 mg/l of picloram in callus induction medium showed high frequency for plant regeneration from mature embryos. Leaf base segments were transferred to callus induction medium and incubated at 25~circC in 16/8 hr light/dark cycle for 3 weeks. Callus induction from leaf base segments of Malgwiri showed high efficiency in medium containing 3 mg/l of 2,4-D and 1 mg/l of kinetin (91.8~%) . In case of Samhangwiri, the combinations of phytohormones did not show significant difference. Regeneration from leaf base segments showed high frequency in shoot medium containing 1 mg/l of antiauxin, tri-iodobenzoic acid (TIBA) and 1 mg/l of 6-benzyladenine (BA). Calli induced from leaf base segments of Samhangwiri and Malgwiri in media containing 3 mg/l of 2,4-D and 3 mg/l of picloram showed high regeneration frequency. It appears that the callus initiation medium may be an important factor for subsequent plant regeneration.

Improvement of Regeneration Efficiency from Mature Embryo and Leaf Base Segment in Korean Oat Genotypes

Kim Kyung-Hee,Moon Jung-Hun,Lee Sang-Kyu,Lee Byung-Moo The Korean Society of Crop Science 2004 Korean journal of crop science Vol.49 No.4

Mature embryo and leaf base segment of Korean oat were used as materials in an experiment to check plant regeneration efficiency. MS media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin, and picloram were used for callus induction from mature embryos and leaf base segments. Three mg/l of 2,4­D and 3 mg/l of picloram in callus induction medium showed high frequency for plant regeneration from mature embryos. Leaf base segments were transferred to callus induction medium and incubated at $25^{\circ}C$ in 16/8 hr light/dark cycle for 3 weeks. Callus induction from leaf base segments of Malgwiri showed high efficiency in medium containing 3 mg/l of 2,4-D and 1 mg/l of kinetin $(91.8\%)$. In case of Samhangwiri, the combinations of phytohormones did not show significant difference. Regeneration from leaf base segments showed high frequency in shoot medium containing 1 mg/l of antiauxin, tri-iodobenzoic acid (TIBA) and 1 mg/l of 6-benzyladenine (BA). Calli induced from leaf base segments of Samhangwiri and Malgwiri in media containing 3 mg/l of 2,4-D and 3 mg/l of picloram showed high regeneration frequency. It appears that the callus initiation medium may be an important factor for subsequent plant regeneration.

Effect of Feeding Different Maturity Leaves and Intermixing of the Leaves on Commercial Characters of Bivoltine Hybrid Silkworm (Bombyx mori L.)

Rahmathulla, V.K.,Raj, Tilak,Himanthraj, M.T.,Vindya, G.S.,Devi, R.G.Geetha Korean Society of Sericultural Science 2003 International Journal of Industrial Entomology Vol.6 No.1

The study evaluated the influence of feeding different maturity leaves i.e., tender, medium, coarse and mixed leaves of three different maturity during late larval stages of new bivoltine race $(CSR_{3} {\times} CSR_{6})$. The data were compared with shoot feeding and control batches in which conventional feeding method was followed. The most of the larval and cocoon characters were recorded significantly higher in tender leaves fed batches followed by T4 batch (2 times tender and 1 time coarse leaves). Lowest melting percentage (1.494%) was recorded in T4 and highest (4.69%) was recorded in coarse leaf (T3) fed batches. Significantly higher post cocoon parameters viz., average filament length, non-breakable filament length, renditta and raw silk percentage were recorded in tender loaves fed batches.

Rapid Propagation of Pelagonium Inquinans Via Organogenesis from Mature Leaf Explants

Hwang, Sung-Jin The Korean Society of Medicinal Crop Science 2006 韓國藥用作物學會誌 Vol.14 No.2

A method for plant regeneration via organogenesis from Pelagonium inquinans leaf disc has been developed. Mature leaf explants were collected from field-grown plants and used for the induction of adventitious shoot regeneration on Murashige and Skoog (MS) medium supplemented with 3% (w/v) sucrose plus plant growth regulators. Maximum shoot organogenesis, with $11.8{\pm}1.5$ shoots (98.6%) per leaf disc, was obtained with $2\;mg/l$ $N^6-benzyladenine$ (BA) and $0.5\;mg/l$ ${\alpha}-naphthyleneacetic$ acid (NAA) in 30 days. For rooting, the in vitro proliferated and elongated shoots were excised into 1.5-2 cm in length microcutting, which were plated individually on an half-strength MS (1/2MS) medium supplemented with 2% (w/v) sucrose plus various concentrations of indole-3-butyric acid (IBA). Shoots rooted with a frequency of 100% following culture on 1/2MS medium containing $0.5\;mg/l$ IBA.

Rapid Propagation of Pelagonium Inquinans Via Organogenesis from Mature Leaf Explants

Sung-Jin Hwang 韓國藥用作物學會 2006 한국약용작물학회지 Vol.14 No.2

A method for plant regeneration via organogenesis from Pelagonium inquinans leaf disc has been developed. Mature leaf explants were collected from field-grown plants and used for the induction of adventitious shoot regeneration on Murashige and Skoog (MS) medium supplemented with 3% (w/v) sucrose plus plant growth regulators. Maximum shoot organogenesis, with 11.8±1.5 shoots (98.6%) per leaf disc, was obtained with 2 mg/l N6-benzyladenine (BA) and 0.5 mg/l α-naphthyleneacetic acid (NAA) in 30 days. For rooting, the in vitro proliferated and elongated shoots were excised into 1.5-2 cm in length microcutting, which were plated individually on an half-strength MS (1/2MS) medium supplemented with 2% (w/v) sucrose plus various concentrations of indole-3-butyric acid (IBA). Shoots rooted with a frequency of 100% following culture on 1/2MS medium containing 0.5 mg/l IBA.

무궁화 품종 '난파'의 효율적인 재분화 체계 확립

손지희,선현진,강홍규,서석철,이효연,Son, Ji-Hi,Sun, Hyeon-Jin,Kang, Hong-Gyu,Suh, Seok-Chul,Lee, Hyo-Yeon 한국식물생명공학회 2019 식물생명공학회지 Vol.46 No.4

Hibiscus syriacus L., the national flower of Korea, is a commonly grown ornamental species found in parks, gardens and along roadsides in Korea. This study sought to establish a plant regeneration system of H. syriacus 'Nanpa' using mature leaves as an explant. Sterilized mature leaf explants were cultured on modified MS medium with combinations of NAA and BAP at various concentrations for 6 weeks. Among the various media evaluated, modified MS media containing 0.46 mg/L NAA and 0.5 mg/L BAP (CI-1) or 0.92 mg/L NAA and 1 mg/L BAP (CI-4) were the most effective for callus formation. Whitish-yellow calluses were observed on CI-1 medium, while green calluses formed on CI-4 medium. The whitish-yellow callus derived from CI-1 medium showed a higher frequency of shoot induction than the green callus derived from CI-4 on modified MS medium containing 0.5 mg/L BAP. Among the various media evaluated in this study, modified MS medium containing 0.46 mg/L NAA and 0.5 mg/L BAP was the most optimal for shoot-forming callus production. Our findings show that mature leaves of H. syriacus 'Nanpa' can be used for in vitro plant regeneration, and the regeneration system described here may be a powerful tool for molecular breeding of H. syriacus 'Nanpa' in the future.

무궁화 품종 ‘난파’의 효율적인 재분화 체계 확립

손지희,선현진,강홍규,서석철,이효연 한국식물생명공학회 2019 JOURNAL OF PLANT BIOTECHNOLOGY Vol.46 No.4

Hibiscus syriacus L., the national flower of Korea, is a commonly grown ornamental species found in parks, gardens and along roadsides in Korea. This study sought to establish a plant regeneration system of H. syriacus ‘Nanpa’ using mature leaves as an explant. Sterilized mature leaf explants were cultured on modified MS medium with combinations of NAA and BAP at various concentrations for 6 weeks. Among the various media evaluated, modified MS media containing 0.46 mg/L NAA and 0.5 mg/L BAP (CI-1) or 0.92 mg/L NAA and 1 mg/L BAP (CI-4) were the most effective for callus formation. Whitish-yellow calluses were observed on CI-1 medium, while green calluses formed on CI-4 medium. The whitish-yellow callus derived from CI-1 medium showed a higher frequency of shoot induction than the green callus derived from CI-4 on modified MS medium containing 0.5 mg/L BAP. Among the various media evaluated in this study, modified MS medium containing 0.46 mg/L NAA and 0.5 mg/L BAP was the most optimal for shoot- forming callus production. Our findings show that mature leaves of H. syriacus ‘Nanpa’ can be used for in vitro plant regeneration, and the regeneration system described here may be a powerful tool for molecular breeding of H. syriacus ‘Nanpa’ in the future.

세장방추형 ‘홍로’ 사과나무의 수관 부위별 수광상태와 잎 및 과실의 특성

송주희(Ju-Hee Song),강인규(In-Kyu Kang),최동근(Dong Geun Choi) 한국원예학회 2014 원예과학기술지 Vol.32 No.4

‘홍로’/M.9 세장방추형 수형에서 수관위치에 따른 광환경과 잎과 과실의 특성을 구명하기 위하여 수관을 상부와 하부, 그리고 내부와 외부로 나누어 조사하였다. 수관상단의 외부가 투광량이 가장 높았고, 광합성속도는 수관하단의 내부보다 약 4.5배 높게 나타났다. 잎의 생장은 수관 내부 잎이 외부 잎보다 더 넓고 얇았으며, 잎의 책상조직 첫번째 층의 두께는 수관외부(110.3㎛)가 수관내부(68.5㎛)보다 두꺼웠다. 과중, 가용성 고형물 함량 및 착색도는 투광률이 높은 수관외부의 과실이 내부보다 높았다. 과실의 가용성 유리당 함량은 수관외부 과실이 내부보다 높았다. 수관내부의 과실은 숙기가 지연되었다. 과피는 수관내부 과실에서 더 두꺼웠고 왁스층의 발달도 많게 나타났다. 따라서 본 연구결과는 수관내부의 광환경을 개선하기 위한 노력과 수관 부위별로 숙기에 따른 분할 수확이 필요함을 제시하였다. For this analysis, canopies of slender-spindle ‘Hongro’/M9 apple trees were divided into 4 positions: upper, lower, exterior, and interior parts. The photosynthetic rate was highest in the external part of the upper canopy of the tree, where it was 4.5 times higher than in the internal part of the lower canopy. This difference was closely associated with differences in light penetration based on canopy position. Analysis of leaf growth characteristics showed that the leaves situated in the internal part of the canopy were larger and thinner than those in the external part of the canopy. The difference in leaf thickness was mainly due to thickness of the primary layer of palisade tissues (68.5 and 110.3 ㎛ for internal and external leaves, respectively). Fruit weight and quality were closely related to the extent of light penetration. Fruit weight, soluble solid content, and red skin color were higher in the fruits from the external part of the canopy. Thus, fruit maturity was delayed in the internal part of canopy. The fruit skin and wax layer were thicker in fruits from the internal canopy than in those from the external canopy. Therefore, our results indicate a need for improved light penetration in internal parts of the canopy and for split harvesting depending on maturity at different canopy positions.