Effect of High Vanillin Treatment on Storage Quality of Fresh-cut Apples

Hun-Sik Chung,Peter M.A. Toivonen,Kwang-Deog Moon 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.3

The effect of post-cut vanillin treatment at high concentrations on changes of quality and microorganism in freshcut apples was studied. Apples (Malus domestica Borkh. cv. Fuji) were sliced, treated by dipping in different vanillin solution, 0, 40, 80, and 120 mM, packed in polyethylene bag, and then stored for up to 3 weeks at 4℃. Changes in total aerobic bacteria, yeast and molds, browning, soluble solids, and titratable acidity during storage were investigated. Growth of total aerobic bacteria throughout storage was strongly inhibited by vanillin regardless of treatment concentrations. Growth of yeast and molds was inhibited by vanillin of all concentrations until 2 weeks of storage. Levels of browning index, soluble solids, and titratable acidity were not significant difference among the treatment conditions until 2 weeks of storage. However, when stored for 3 weeks, browning index increased more at 80 or 120 mM vanillin, while soluble solids and titratable acidity more be decreased by 120 mM vanillin as compared with other treatment conditions. These results show that the usage of vanillin in processing of fresh-cut apples had a limitation for maintaining quality.

S-allele Specific PCR 분석에 의한 사과와 꽃사과 품종의 자가불화합성 유전자형 동정

조강희,김정희,이정우,권순일,박종택,신일섭,김세희,김대현,최인명 한국육종학회 2014 한국육종학회지 Vol.46 No.4

사과는 배우체형 자가불화합성을 나타내는데 이는 S-locus의 복대립유전자에 의해 조절된다. 본 연구는 S-allele specific PCR분석을 통해 신품종을 포함한 24종의 사과 주요 재배품종과 7종의 꽃사과 품종의 자가불화합성 유전자형(S-genotype)을 결정하고자 수행하였다. 31종의 재배품종과 꽃사과 품종 을 23종의 S-allele specific primer을 이용하여 분석한 결과 12개의 S-allele (S1, S2, S3, S5, S7, S9, S10, S16, S21, S23, S26, S29)이 동정되었다. 그 중에서 24종의 재배품종에는 S1(41.7%), S3(58.3%), S7(29.2%), S9(54.2%)의 S-allele이 흔히 존재하는 것으로 확인되었다. 국내육성 신품종인 ‘아리수’와 ‘황옥’의 S-genotype은 각각 S3S7과 S3S9으로 동정되었다. 본 실험에서 얻은 S-genotype 정보는 안정적인 사과 과실생산에 적합한 수분수 선발과 육종프로그램에서 교배조합 작성에 유용 하게 활용될 것이다. Apple (Malus ☓ domestica Borkh.) has gametophytic self-incompatibility (S) controlled by the multi-allelic S-locus. In the present study, S-genotypes of 24 apple cultivars including newly released Korean cultivars and seven crabapple cultivars were identified using S-allele specific polymerase chain reaction analysis. Twelve different S-alleles (S1, S2, S3, S5, S7, S9, S10, S16, S21, S23, S26, and S29) from 31 apple and crabapple cultivars were identified using 23 S-allele specific primers. Among them, S1 (41.7%), S3 (58.3%), S7 (29.2%), and S9 (54.2%) S-alleles were found to be common in 24 apple cultivars. The newly released Korean cultivars ‘Arisoo’ and ‘Hwangok’ were genetyped as S3S7 and S3S9, respectively. S-genotypes information obtained from the present study will be useful to select proper pollinzers for stable production of apple fruit and to design cross of breeding programs.

생육기 조기낙엽에 따른 사과 ‘후지’/M9의 광합성과 탄수화물의 변화

정재훈(Jae Hoon Jeong),한점화(Jeom Hwa Han),류수현(Suhyun Ryu),한현희(Hyun Hee Han),권용희(YongHee Kwon),도경란(Gyung-Ran Do),임순희(Sun-Hee Yim),이한찬(Han-Chan Lee) (사)한국생물환경조절학회 2017 시설원예‧식물공장 Vol.26 No.4

본 연구에서는 사과 ‘후지’/M9의 생육기간 중 조기낙엽 피해가 발생하였을 때 영향을 알아보기 위하여 인위적으로 적엽 처리하여 광합성과 탄수화물의 변화를 측정하였다. 눈의 재발아율은 대조구, 50% 처리구, 80% 처리구에서 각각 2.1%, 45.9%, 82.7%로 매우 유의한 차이를 나타냈다. 적엽처리 후 남은 잎의 자당(sucrose)와 전분 함량을 측정한 결과 모든 처리구에서 유의한 차이는 없었다. 또한 대조구와 50% 처리구에서는 총 유리당의 변화도 크지 않았다. 반면 80% 처리구에서 총 유리당과 sorbitol의 함량은 처리 후 14일동안 꾸준히 감소하였으며, 이것은 대조구에 비하여 높은 sink로서의 탄수화물 요구가 일어난 것으로 추정된다. 뿌리에서의 전분 축적은 적엽처리 정도가 심할수록 감소하였으나 유의한 차이는 없었다. 광합성속도와 기공전도도는 80% 처리구에서 처리 후 14일동안 꾸준히 유의하게 증가하였다. 광합성 속도의 상승은 남아있는 source 잎에서의 삼투포텐셜이 증가하여 발생한 것으로 판단된다. 이는 적엽처리로 인하여 눈의 당년 2차 재발아에 필요한 sink의 탄수화물 요구가 갑작스럽게 일어나게 됨으로써 남아있는 잎에서 광합성이 증가한 것으로 추정된다. This study was conducted to evaluate the influence of early defoliation on photosynthesis and carbohydrate reserves when the source leaves of ‘Fuji’/M9 apple (Malus domestica Borkh.) trees were removed during the growing period. Bud regrowth rates of 80%, 50% defoliation and non-defoliation treatments were significantly different 82.7%, 45.9% and 2.1% respectively at 30 days after treatment. In all treatments, sucrose and starch concentrations in remaining leaves decreased non-significantly during the 14-day period. No significant changes were observed for total soluble carbohydrates in non-defoliation and 50% defoliation. However, in 80% defoliated treatments, concentrations of sorbitol and total soluble carbohydrates in remaining leaves declined steadily during the 14-day period. It is thought that high sink strength increases the requirements of carbohydrate from remaining leaves more than non-defoliated. The concentrations of starch in the roots tend to decrease non-significantly as percentage of defoliation increased. Photosynthesis of remaining leaves was monitored during the 14-day period after partial defoliation treatments. Net photosynthetic rates (Pn) and stomatal conductance were significantly enhanced in the 80% defoliation. The observed photosynthetic enhancement following partial defoliation may have been due to the enhancement of osmotic potential in leaves. These results were estimated that increasing of photosynthetic rate in the partial defoliation is due to the sink carbohydrate requirements for the current year"s secondary growth of buds.

Influence of Grass Cover on Water Use and Shoot Growth of Young 'Fuji'/M.26 Apple Trees at Three Soil Water Regimes in Double Pot Lysimeters

노희명,Ro, Hee-Myong 한국토양비료학회 1999 한국토양비료학회지 Vol.32 No.4

This study measures the influence of grass cover on water use and shoot growth of apple trees growing under different soil water regimes in temperate climate conditions and evaluates monthly crop coefficients of such conditions during four months of the growing season in 1995. To do so, double pot lysimeter experiments of 3-year-old Fuji' apple (Males domestica Borkh.) trees under a transparent rain shield were designed and installed. Trees were triplicate under three soil water regimes: (A) drip-irrigation at -50 kPa of soil matric potential (IR50). (B) drip-irrigation at -80 kPa of soil matric potential (IR80), and (C) constant shallow water table at 0.45 m below the soil surface (WT45). In each treatment, two soil surface conditions were tested: the soil surface bare, and covered with turf grasses. Mean monthly water use increased with increasing soil matric potential for drip irrigation and was greatest in the WT45 treatment. Monthly crop coefficients increased linearly in time for drip-irrigated apple trees ($r^2$ values of $0.953^{***}$ for turf grass-covered system and of $0.862^{***}$ for bare surface system), while those obtained in the WT45 treatment fluctuated, Duncan's multiple range tests for shoot growth showed that grass-covered IR50 was most favorable to apple trees. while bare surface waterlogged situation was most adverse at least in part due to a lack of oxygen in the root zone. Mid-season leaf Kjeldahl-N was higher in drip-irrigated apple trees than in WT45 trees, while soil Kjeldahl-N was not different irrespective of treatments. 본 연구는 사과나무의 수분이용, 신초 생장 및 작물계수에 미치는 토양수분영역과 잔디 피복의 영향을 조사하였다. 이를 위해 비가림 시설하에 3년생 사과 '후지'/M. 26 (Malus domestica Borkh.)을 재식한 3반복 double pot-lysimeter 실험을 수행하였다: (A) 토양 매트릭 포텐셜 -50 kPa의 점적관수 (IR50), (B) 토양 매트릭 포텐셜 -80 kPa의 점적관수 (IR80), 및 (C) 지하 0.45 m의 정체 자유수면 (WT45), 각 처리에 나지와 잔디 피복의 두 가지 지표상태를 두었다. 평균 월별 수분이용은 IR50 처리에서 IR80 처리보다 컸으며, WT45 처리에서 가장 컸다. 점적관수 처리 사과나무의 월별 작물계수는 시간의 1차 함수로 증가하였으나 ($r^2=0.953^{***}$, 잔디 피복 $r^2=0.862^{***}$, 나지), WT45 처리 사과나무의 작물계수는 변동하였다. 신초생장은 잔디 피복 IR50 처리에서 가장 컸으며, 나지 WT45 처리에서 가장 작았다. 토양의 Kjeldahl 질소는 처리에 관계없이 동일하였으나 8월 10일 측정한 엽중 Kjeldahl 질소는 WT45 처리보다 점적관수 처리에서 높았다.

Applications of Organic Fungicides Reduce Photosynthesis and Fruit Quality of Apple Trees

Narayan Bhusal,Jun Hyung Kwon,Su-Gon Han,Tae-Myung Yoon 한국원예학회 2016 원예과학기술지 Vol.34 No.5

Two different pest control programs were applied on 8-year-old ‘Ryoka’/M.26 apple trees (Malus domestica Borkh.). Lime sulfur or Bordeaux mixture with emulsified oil were applied 12 times from late March to mid-September as organic treatment, and synthetic chemicals were 7 times applied as control treatment. Over the entire apple-growing season, photosynthesis rates of apple trees were significantly lower in the organic treatment than in the control, and this photosynthetic differences were larger in July and August. Photosynthesis-related parameters such as stomatal conductance and transpiration behaved similarly to photosynthesis. The leaf area in the organic treatment was significantly smaller (24.7 ㎠) than that in the control treatment (30.7 ㎠). Organic leaves contained significantly less Chl. a (15.5 ㎎·g<SUP>-1</SUP>) than did control leaves (17.6 ㎎·g<SUP>-1</SUP>). Fruit yield per tree was significantly lower in the organic treatment (18.8 ㎏) than in the control (24.5 ㎏), because organic fruits experienced a higher rate of disease infection such as white rot (Botryosphaeria dothidae ) and bitter rot (Glomerella cingulata ) than did control fruits. Organic fruits had high flesh firmness but less color development (lower Hunter’s a values). In this experiment, the pest control program with frequent applications of organic fungicides showed negative effects on photosynthesis and disease infection on leaves and fruits, and thus reduce the fruit quality and yield in ‘Ryoka’/M.26 apple trees.

Applications of Organic Fungicides Reduce Photosynthesis and Fruit Quality of Apple Trees

Bhusal, Narayan,Kwon, Jun Hyung,Han, Su-Gon,Yoon, Tae-Myung Korean Society of Horticultural Science 2016 원예과학기술지 Vol.34 No.5

Two different pest control programs were applied on 8-year-old 'Ryoka'/M.26 apple trees (Malus domestica Borkh.). Lime sulfur or Bordeaux mixture with emulsified oil were applied 12 times from late March to mid-September as organic treatment, and synthetic chemicals were 7 times applied as control treatment. Over the entire apple-growing season, photosynthesis rates of apple trees were significantly lower in the organic treatment than in the control, and this photosynthetic differences were larger in July and August. Photosynthesis-related parameters such as stomatal conductance and transpiration behaved similarly to photosynthesis. The leaf area in the organic treatment was significantly smaller ($24.7cm^2$) than that in the control treatment ($30.7cm^2$). Organic leaves contained significantly less Chl. a ($15.5mg{\cdot}g^{-1}$) than did control leaves ($17.6mg{\cdot}g^{-1}$). Fruit yield per tree was significantly lower in the organic treatment (18.8 kg) than in the control (24.5 kg), because organic fruits experienced a higher rate of disease infection such as white rot (Botryosphaeria dothidae) and bitter rot (Glomerella cingulata) than did control fruits. Organic fruits had high flesh firmness but less color development (lower Hunter's a values). In this experiment, the pest control program with frequent applications of organic fungicides showed negative effects on photosynthesis and disease infection on leaves and fruits, and thus reduce the fruit quality and yield in 'Ryoka'/M.26 apple trees.

Wax Composition of ‘Red Fuji’ Apple Fruit during Development and during Storage after 1-Methylcyclopropene Treatment

Xiaoqing Dong,Jingping Rao,Donald J. Huber,Xiaoxiao Chang,Fucun Xin 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.4

Changes in surface wax composition of ‘Red Fuji’ apple (Malus domestica Borkh. ‘Red Fuji’) during development and during storage at 0 ± 1℃ after 1-methylcyclopropene (1-MCP) treatments were studied by the means of gas chromatography -mass spectrometry. Total wax increased during fruit development. During the storage at 0 ± 1℃, total waxes declined, and the decline was significantly delayed in 1-MCP–treated fruit. Total waxes were chromatographically separated into nonpolar and polar components. Nonacosane was the most abundant nonpolar wax, comprising 95% of total hydrocarbons. Polar wax components were comprised of nonacosan-10-ol (29%) and nonacosan-10-one (16%), along with a series of fatty acids and derivatives. Distinct patterns of wax changes were observed. Nonacosane, heptacosane and nonacosene increased during the development and decreased over seven months of fruit storage at 0 ± 1℃. Declines were delayed or slightly suppressed in 1-MCP–treated fruit. By contrast, nonacosan-10-ol, nonacosan-10-one, and 9, 12-octadecadienoic acid showed variable accumulation trends during the development but significant increases during storage that were strongly suppressed in 1-MCP–treated fruit. The association of wax changes with ethylene production and the responses to 1-MCP indicate that ethylene strongly influences wax composition in ‘Red Fuji’ apple fruit.

Chill Unit Models for Predicting Dormancy Completion of Floral Buds in Apple and Sweet Cherry

곽성희,Denise Neilsen 한국원예학회 2013 Horticulture, Environment, and Biotechnology Vol.54 No.1

Temperature-controlled experiments were conducted to determine the relative contribution of temperatures to dormancy completion of floral buds in ‘Gala’ apple (Malus domestica Borkh.) and ‘Sweetheart’ sweet cherry (Prunus avium L.). Shoots were collected right before chill inception, and exposed continuously to seven different temperatures in the range of -2°C and 16.8°C for 1320 hours. Chilling requirements were determined on sequential field samples from plots of days to bud break against sampling date. Temperature-response curves, which were best fit in a four parameter logistic nonlinear regression, indicated that optimum chilling temperatures were between -2°C and 5.5°C for ‘Gala’ and between -2°C and 7°C for ‘Sweetheart’, with no chilling effect above 13°C in both species. The derived chill unit models predicted that chill unit accumulations required for dormancy completion averaged 921 for ‘Gala’and 740 for ‘Sweetheart’ over the three year period. The year-to-year variation in ‘Gala’ apple was smaller with our ‘Gala’ model than with the ‘Utah’ model (14% vs 23.5% coefficients of variation), while similar to each other in ‘Sweetheart’ sweet cherry.

GA4+7+BA의 처리방법이 사과 ‘갈라’ 품종의 수체생장 및 과실특성에 미치는 영향

사공동훈,윤태명,최석원 한국원예학회 2010 원예과학기술지 Vol.28 No.4

GA4+7+BA, a plant growth regulator for induction of feathering in young apple tree and increasing fruit size, was applied by various methods on ‘Gala’/M.9 apple trees in high density orchard for 4 years to investigate its effect on fruit and shoot growth. GA4+7+BA(80-300 mg・L-1) increased fruit length, fruit weight, and L/D ratio regardless of application methods, but it did not affect soluble solid content, acidity, leaf area, and chlorophyll. Seed number was not affected by GA4+7+BA application, however, more immature seeds was observed in treated ‘Gala’ fruit. Shoot growth was increased when spraying GA4+7+BA at tree crown but not affected when spraying at fruit directly. More significant fruit growth was observed when GA4+7+BA was applied on the fruits between late of May and early of June when fruit cell division ended; however, high concentration of GA4+7+BA resulted in lower fruit storability because of lower firmness. Hence, more attention should be paid when applying high concentration of GA4+7+BA to small sized fruit cultivars like ‘Gala’. GA4+7+BA는 유목의 측지 발생 및 과실비대를 목표로 사용되는 생장조절제로써 본 실험에서는 밀식 과수원의 ‘갈라’ 사과품종을 공시하여 4년 동안 여러 가지 살포방법을 이용하여 과실비대 및 영양생장에 미치는 영향을 조사하였다. GA4+7+BA 처리시 과실 종경, 과중, L/D율은 증가되나 가용성 고형물 함량 및 산 함량, 엽면적, 엽록소함량 등은 변화가 일정하지 않았다. 과실 내 종자 수는 처리에 따른 차이가 없었으나 미발육 종자의 수가 증가하는 것으로 나타났다. 고농도 수관전체 살포는 신초생장을 증가시켰으나 착과 부위에만 살포시에는 영향이 없었다. 살포시기에 있어서는 과실 세포분열이 끝나는 시점인 5월말에서 6월초에 살포하는 것이 과실비대에 가장 효과적이었다. 그러나 고농도 GA4+7+BA 처리는 과실의 경도를 감소시켜 과실의 저장성을 떨어뜨리기 때문에 ‘갈라’ 같은 소과종에 있어 고농도의 GA4+7+BA 살포는 주의가 필요할 것으로 판단되었다.

갈색무늬병에 의한 낙엽이 '후지'/M.9 사과나무의 수체생장 및 과실품질에 미치는 영향

사공동훈(Dong-Hoon Sagong),권헌중(Hun-Joong Kweon),송양익(Yang-Yik Song),박무용(Moo-Yong Park),남종철(Jong-Chul Nam),강석범(Seok-Beom Kang),이상규(Sang-Gyu Lee) 한국원예학회 2011 원예과학기술지 Vol.29 No.6

본 실험은 ‘후지’/M.9 사과나무의 갈색무늬병에 의한 낙엽 정도가 수체생장과 과실품질에 미치는 영향을 조사하고자 실시하였다. 갈색무늬병에 의해 9월말 수관 전체 낙엽율이 10% 이상 되면 가용성고형물 함량이 감소되었고, 30% 이상이면 평균과중이 감소되었다. 과실의 착색과 전분 함량은 과실 주변 잎들의 낙엽 정도가 심해질수록 감소되는 경향을 나타내었다. 이듬해 개화율 및 과중, 총신초장은 전년도 낙엽율이 높을수록 감소되는 경향이 있었다. ‘후지’/M.9 사과나무의 과대지에서 갈색무늬병의 발생이 없는 잎은 9월말에도 광합성속도를 약 10μmol?m-2?s-1 이상 유지하여 9월말 이후의 과실비대 및 가용성고형물 함량 증가에 영향을 미쳤다. 9월말 갈색무늬병반이 전체 잎면적의 50% 이상을 차지한 잎의 광합성속도는 건전한 잎의 30% 수준이었다. 결론적으로 낙엽구들의 과실품질 감소는 과실 내 전분 감소에 의해서 이며, 과실 내 전분 감소는 갈색무늬병에 의해 과실주변 잎들의 광합성속도가 8월 이후부터 감소되었기 때문이었다. This study was carried out to investigate the influence of defoliation by Marssonina blotch (Diplocarpon mali Harada et Sawamura) on vegetative growth and fruit quality in ‘Fuji’/M.9 apple tree. Soluble solid contents decreased when the defoliation percentage by Marssonina blotch was over 10% before the end of September, and fruit weight decreased when percentage of defoliation was over 30%. Fruit red color and starch contents tend to decrease as percentage of defoliation near the fruit increased. Return bloom, fruit weight, and shoot growth the following year tend to decrease as percentage of defoliation increased. Photosynthetic rate of healthy leaves in bourse shoot during the end of September was maintained about 10 μmol?m-2?s-1, effects in increasing fruit growth and soluble solid contents after the end of September. Photosynthetic rates for the damaged leaf, damaged area was over 50% on the leaf surface, while 30% of the photosynthetic rates of healthy leaf are without damage applied with Marssonina b lotch at the end of September. The results show that the decrease of fruit quality in defoliation treatments may be caused by the decrease of starch contents in fruit, and that was caused by the photosynthetic rates of leaves near fruit was decreased by Marssonina blotch in the wake of August.