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Lee Yunjeong,Jaikwang Nattira,Kim Seong keun,Jeong Jiseon,Sukhoom Ampaitip,Kim Jong-Hwa,Kim Wonyong 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.5
Safety assessment and functional analysis of probiotic candidates are important for their industrial applications. Lactiplantibacillus plantarum is one of the most widely recognized probiotic strains. In this study we aimed to determine the functional genes of L. plantarum LRCC5310, isolated from kimchi, using next-generation, whole-genome sequencing analysis. Genes were annotated using the Rapid Annotations using Subsystems Technology (RAST) server and the National Center for Biotechnology Information (NCBI) pipelines to establish the strain’s probiotic potential. Phylogenetic analysis of L. plantarum LRCC5310 and related strains showed that LRCC5310 belonged to L. plantarum. However, comparative analysis revealed genetic differences between L. plantarum strains. Carbon metabolic pathway analysis based on the Kyoto Encyclopedia of Genes and Genomes database showed that L. plantarum LRCC5310 is a homofermentative bacterium. Furthermore, gene annotation results indicated that the L. plantarum LRCC5310 genome encodes an almost complete vitamin B6 biosynthetic pathway. Among five L. plantarum strains, including L. plantarum ATCC 14917T, L. plantarum LRCC5310 detected the highest concentration of pyridoxal 5’-phosphate with 88.08 ± 0.67 nM in MRS broth. These results indicated that L. plantarum LRCC5310 could be used as a functional probiotic for vitamin B6 supplementation.
Safety Assessment of Lactiplantibacillus (formerly Lactobacillus) plantarum Q180
( Yoo Jin Kwon ),( Byung Hee Chun ),( Hye Su Jung ),( Jaeryang Chu ),( Hyunchae Joung ),( Sung Yurb Park ),( Byoung Kook Kim ),( Che Ok Jeon ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.10
The safety of the probiotic strain Q180, which exerts postprandial lipid-lowering effects, was bioinformatically and phenotypically evaluated. The genome of strain Q180 was completely sequenced, and single circular chromosome of 3,197,263 bp without any plasmid was generated. Phylogenetic and related analyses using16S rRNA gene and whole-genome sequences revealed that strain Q180 is a member of Lactiplantibacillus (Lp., formerly Lactobacillus) plantarum. Antimicrobial resistance (AMR) genes were bioinformatically analyzed using all Lp. plantarum genomes available in GenBank, which showed that AMR genes are present differently depending on Lp. plantarum strains. Bioinformatic analysis demonstrated that some mobile genetic elements such as prophages and insertion sequences were identified in the genome of strain Q180, but because they did not contain harmful genes such as AMR genes and virulence factor (VF)- and toxin-related genes, it was suggested that there is no transferability of harmful genes. The minimum inhibition concentrations of seven tested antibiotics suggested by the European Food Safety Authority guidelines were slightly lower than or equal to the microbiological cut-off values for Lp. plantarum. Strain Q180 did not show hemolytic and gelatinase activities and biogenic amine-producing ability. Taken together, this study demonstrated the safety of strain Q180 in terms of absence of AMR genes and VF- and toxin-related genes as a probiotic strain.
김병용,Bonggyu Min,Yoo-Jin Kwon,SUNG-YURB PARK,Jong Hyun Lim,Chang-Hun Shin,Byoung Kook Kim 건강기능식품미래포럼 2022 건강기능식품미래포럼 학술지 Vol.2 No.4
Lactiplantibacillus plantarum CKDB008 was isolated from a Korean fermented food, kimchi. In order to investigate its potential as a probiotic in the food industry, the genome sequence of L. plantarum CKDB008 was analyzed to confirm its probiotic properties and safety at the genome level. The CKDB008 strain has one circular chromosome of 3.2 Mbp, with 44.6% guanine-cytosine content. A comparative genomic analysis confirmed that its genome was similar to those of 19 L. plantarum strains used as probiotics. Pan-genome analysis of 20 L. plantarum strains identified 6,236 genes, including 2,006 core, 255 soft core, 1,485 shells, and 2,490 cloud genes. These results reveal the ‘open’ nature of L. plantarum pan-genome. Among the predicted 3,129 protein coding genes, the genome contains genes associated with important characteristics of probiotics such as protease system, amino acid biosynthesis, major carbohydrate-active enzymes, stress resistance, and cell adhesion. In addition, genes encoding bacteriocins, immunoregulatory, and gamma-aminobutyric acid biosynthesis were also identified, but safety-related genes related to antibiotic resistance and virulence factors were not. The results obtained strongly suggest that CKDB008 strain is genetically safe and has the desired properties required for a probiotic as well as support that L. plantarum CKDB008 strain has a potential as a probiotic that can be used for the industrial purpose.
Jeong Jiseon,Lee Yunjeong,Yoon Seokmin,Kim Jong-Hwa,Kim Wonyong 한국미생물학회 2021 The journal of microbiology Vol.59 No.12
As the functions of probiotics within the same species may not be shared, it is important to analyze the genetic characteristics of strains to determine their safety and usefulness before industrial applications. Hence the present study was undertaken to determine functional genes, and beneficial activities of strain LRCC5314, a bacterial strain isolated from kimchi through comparative genomic analysis. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain LRCC5314 was a member of the species L. plantarum. Whole genome size of strain LRCC5314 was sequence was 3.25 Mb long, with a G + C content of 44.5 mol% and 3,031 predicted genes. Strain LRCC5314 could metabolize hexoses through homofermentation, which produces only lactic acid from hexoses. According to gene annotation, strain LRCC- 5314 contained genes of EPS production and CRISPR. Moreover, the strain contained genes that could encode a complete biosynthetic pathway for the production of tryptophan, which can be used as a precursor of serotonin. Notably, the tryptophan and serotonin activities strain LRCC5314 were higher than those of reference strains, L. plantarum ATCC 14917T, DSM 20246, DSM 2601, and ATCC 8014, which reach tryptophan amount of 0.784 ± 0.045 μM/ml in MRS broth and serotonin concentration of 19.075 ± 0.295 ng/ml in HT-22 cells. These findings indicated that L. plantarum LRCC5314 could provide a source for serotonin production and could be used as a functional probiotic for stress regulation.
김치 유래 Lactiplantibacillus plantarum THK-j112의 Streptococcus mutans에 대한 항균효과 및 항생물막 효과
유두나(Du-Na Yu),이은지(Eun-Ji Yi),정제용(Je-Yong Jung),이태후(Tae-Hoo Yi),국무창(Moochang Kook) 한국유산균프로바이오틱스학회 2023 Current Topic in Lactic Acid Bacteria and Probioti Vol.9 No.1
Dental caries is an infectious disease accompanied by the destruction of teeth. It is a multifactorial disease caused by the interaction between microbes in dental plaque and food, with cariogenic Streptococcus mutans being one of the primary causes. In this study, the antimicrobial and antibiofilm activities of Lactiplantibacillus plantarum THK-j112 isolated from Kimchi, a traditional fermented food, against S. mutans were investigated. Furthermore, this paper also showed the possibility of developing this strain. After analyzing the 16S rRNA sequence, strain THK-j112 was named L. plantarum THK-j112 as it shared 99.93% similarity with L. plantarum ATCC 14917T. Both L. plantarum THK-j112 and L. plantarum ATCC 14917T demonstrated MIC values of 5 mg/mL and MBC values of 10 mg/mL against S. mutans KACC 16833T. The study confirmed that L. plantarum THK-j112 exhibited significant concentration-dependent inhibitory effects on biofilm formation, acid production, and glucosyltransferases(GTFs) production. Regarding hemolytic activity, Bacillus cereus ATCC 14579T, used as a positive control, was confirmed to cause hemolysis, whereas L. plantarum THK-j112 did not cause this phenomenon. Additionally, except for gentamicin, the antibiotic resistance test revealed a lower value than the MIC cut-off value for each antibiotic, indicating no resistance. Based on the findings, L. plantarum THK-j112 is expected to be useful in the food industry, such as functional food, as a lactic acid bacterium with antimicrobial and antibiofilm effects.
Zhang Di,Shin Heesung,Wang Tingting,Zhao Yaxin,Lee Suwon,Lim Chongyoon,Zhang Shiqi 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.8
Lactiplantibacillus plantarum, previously named Lactobacillus plantarum, is a facultative, homofermentative lactic acid bacterium widely distributed in nature. Several Lpb. plantarum strains have been demonstrated to possess good probiotic properties, and Lpb. plantarum HOM3204 is a potential probiotic strain isolated from homemade pickled cabbage plants. In this study, wholegenome sequencing was performed to acquire genetic information and predict the function of HOM3204, which has a circular chromosome of 3,232,697 bp and two plasmids of 48,573 and 17,060 bp, respectively. Moreover, various oxidative stress-related genes were identified in the strain, and its antioxidant activity was evaluated in vitro and in vivo. Compared to reference strains, the intracellular cell-free extracts of Lpb. plantarum HOM3204 at a dose of 1010 colony-forming units (CFU)/ml in vitro exhibited stronger antioxidant properties, such as total antioxidant activity, 2,2- diphenyl-1-picrylhydrazyl radical scavenging rate, superoxide dismutase activity, and glutathione (GSH) content. Daily administration of 109 CFU Lpb. plantarum HOM3204 for 45 days significantly improved the antioxidant function by increasing the glutathione peroxidase activity in the whole blood and GSH concentration in the livers of D-galactose-induced aging mice. These results suggest that Lpb. plantarum HOM3204 can potentially be used as a food ingredient with good antioxidant properties.
김치 유래 Lactiplantibacillus plantarum 사균체의 항산화 및 항염 효과
유청빈,이은수,이민경,이가영,박훈 한국유산균프로바이오틱스학회 2022 Current Topic in Lactic Acid Bacteria and Probioti Vol.8 No.2
The aim of this study was to evaluate the probiotic properties of lactic acid bacteria (LAB) isolated from kimchi and antioxidant and anti-inflammatory activities of heat-killed LAB strains. Regarding probiotic properties, Lactiplantibacillus plantarum SMF398 and L. plantarum SMF470 isolated from kimchi can survive under artificial gastric condition and adhere strongly to HT-29 cells. The SMF398 and SMF470 strains showed strong antimicrobial activity and co-aggregation against pathogenic bacteria. The heat-killed cells of LAB (1 mg/mL) were prepared by heating at 121℃ for 15 min. MTT assay revealed that the heat-killed cells (1 mg/mL) of SMF398 and SMF470 were not toxic to HT-29 cells. The heat-killed SMF398 and SMF470 showed significantly higher DPPH and ABTS radical scavenging activities as well as β- carotene bleaching inhibitory activity than the heat-killed L. plantarum ATCC14917, a control probiotic strain (p<0.05). In lipopolysaccharide-induced RAW264.7 cells, the heat-killed SMF398 and SMF470 significantly reduced the nitric oxide production by 30.92% and 22.81%, respectively (p<0.05). Furthermore, the heat-killed SMF398 and SMF470 significantly decreased the gene expression levels of tumor necrosis factor-α, interleukin-6, inducible nitric oxide synthase, cyclooxygenase-2 up to 58.02%, 67.70%, 53.44%, 45.90%, respectively (p<0.05). These results suggest that the heat-killed L. plantarum SMF398 and L. plantarum SMF470 might be useful as antioxidant and anti-inflammatory agents.
한국 오이김치에서 분리한 Lactiplantibacillus plantarum GD00040 (KCCM 43412) 유산균주의 유전체 분석
최서진,김은배 한국미생물학회 2021 미생물학회지 Vol.57 No.3
Lactiplantibacillus plantarum is a beneficial probiotic for humans and commonly used as a feed additive for animals. We sequenced the genome of Lpb. plantarum GD00040 isolated from Korean cucumber kimchi using the MGIseq system. The total length of the draft genome was 3,392,132 bp, and its G + C content was 44.2% in 122 contigs (length ≥ 500 bp). Lactiplantibacillus plantarum contained a plasmid of length 3,962 bp, with G + C content of 35.9%. 이 연구에서 사용한 Lactiplantibacillus plantarum GD00040 (KCCM 43412) 균주는 한국 전통 발효식품인 오이김치에서 분리되었다. 우리는 이 균주에 대하여 WGS를 진행하였다. 유 전체 초안의 크기는 3,392,132 bp, G + C 함량(%)는 44.2%이 고 500 bp 이상의 contig 122개로 조립되었다. 그리고 원형의 플라스미드 3,962 bp, G + C 함량(%)이 35.9%가 발견되었다. 유전체 초안에서 항균활성물질(Bacteriocin)인 Plantaricin과 Pediocin 유전자가 확인되었다. 또한 알려진 항생제 저항성 유 전자는 발견되지 않았다.