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자연폐경여성에서 interleukin(IL)-6 유전자내 (CA) 반복다형성, 혈청 IL-6농도 및 골밀도와의 연관성
김정구(Jung Gu Kim),김석현(Seok Hyun Kim),최영민(Young Min Choi),문신용(Shin Yong Moon),이진용(Jing Yong Lee) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.3
N/A Objective: To investigate the relationship between cytosine-adenosine(CA) polymorphism in interleukin-6 (IL-6) gene, serum IL-6 levels and bone mineral density (BMD). Methods : IL-6 CA polymorphism was analyzed by polyacrylamide-urea gel electrophoretic patterns in 300 postmenopausal Korean women. and direct DNA sequencing. Serum IL-6 was measured by enzyme linked immunosorbent assay, and BMD at the lumbar spine and proximal femur by dual energy X-ray absorptiometry. Results: Nine alleles were observed with product sizes ranging between 120-136 bp and 125 allele was the most common. There were no significant differences in serum IL-6 levels and BMD at the lumbar spine and proximal femur between the women who possessed at least one allele of each genotype and those who did not posses that allele. Serum IL-6 levels did not correlated with age, menopause duration, body mass index and BMD at the lumbar spine and proximal femur. IL-6 CA polymorphic pattern and serum IL-6 levels in osteoporotic women were not different from those in normal BMD or osteopenic women. Conclusions: IL-6 CA polymorphism has no relationship with serum IL-6 levels and BMD in postmenopausal Korean women.
Gang-Min Hur.Yin-Bang Hwang,Jae-Heun Lee,So-Hyun Bae,Ji-Sun Park,Choong-Jae Lee,Jeong-Ho Seok 대한생리학회-대한약리학회 2003 The Korean Journal of Physiology & Pharmacology Vol.13 No.4
To gain insight on the role of pro-inflammatory cytokines in the pathogenesis and treatment of rheumatoid arthritis (RA), the phorbol 12-myristate 13-acetate (PMA)-induced IL-6 gene expression and the effect of caffeic acid phenethyl ester (CAPE) on the PMA-induced IL-6 gene expression were investigated in human fibroblast-like synoviocytes (FLSs). Synovial tissue samples were obtained from rheumatoid arthritis patients, and FLSs were isolated. The cells were stimulated with PMA (100 nM) for 6 hrs to induce IL-6 gene. The cells were pretreated with CAPE (20, 50, 100<FONT FACE= 바탕 >μM) prior to PMA treatment. PMA increased IL-6 RNA expression, binding activities of transcription factors (NF-<FONT FACE= 바탕 >κB, AP-1) to IL-6 promoter, and IL-6 promoter activity. However, CAPE inhibited PMA-induced IL-6 mRNA expression in dose-dependent manner, and also inhibited the increased binding activities of transcription factors to IL-6 promoter and IL-6 promoter activity. These results suggest that CAPE might regulate PKC-mediated IL-6 expression and inflammatory reactions in RA.
Hur, Gang-Min,Hwang, Yin-Bang,Lee, Jae-Heun,Bae, So-Hyun,Park, Ji-Sun,Lee, Choong-Jae,Seok, Jeong-Ho The Korean Society of Pharmacology 2003 The Korean Journal of Physiology & Pharmacology Vol.7 No.6
To gain insight on the role of pro-inflammatory cytokines in the pathogenesis and treatment of rheumatoid arthritis (RA), the phorbol 12-myristate 13-acetate (PMA)-induced IL-6 gene expression and the effect of caffeic acid phenethyl ester (CAPE) on the PMA-induced IL-6 gene expression were investigated in human fibroblast-like synoviocytes (FLSs). Synovial tissue samples were obtained from rheumatoid arthritis patients, and FLSs were isolated. The cells were stimulated with PMA (100 nM) for 6 hrs to induce IL-6 gene. The cells were pretreated with CAPE (20, 50, $100{\mu}M$) prior to PMA treatment. PMA increased IL-6 RNA expression, binding activities of transcription factors ($NF-{\kappa}B$, AP-1) to IL-6 promoter, and IL-6 promoter activity. However, CAPE inhibited PMA-induced IL-6 mRNA expression in dose-dependent manner, and also inhibited the increased binding activities of transcription factors to IL-6 promoter and IL-6 promoter activity. These results suggest that CAPE might regulate PKC-mediated IL-6 expression and inflammatory reactions in RA.
Hur, Gang-Min,Hwang, Yin-Bang,Lee, Jae-Heun,Bae, So-Hyun,Park, Ji-Sun,Lee, Choong-Jae,Seok, Jeong-Ho 충남대학교 암연구소 2005 암연구소 업적집 Vol.4 No.-
To gain insight on the role of pro-inflammatory cytokines in the pathogenesis and treatment of rheumatoid arthritis (RA), the phorbol 12-myristate 13-acetate (PMA)-induced IL-6 gene expression and the effect of caffeic acid phenethyl ester (CAPE) on the PMA-induced IL-6 gene expression were investigated in human fibroblast-like synoviocytes (FLSs). Synovial tissue samples were obtained from rheumatoid arthritis patients, and FLSs were isolated. The cells were stimulated with PMA (100 nM) for 6 hrs to induce IL-6 gene. The cells were pretreated with CAPE (20, 50, 100 μM) prior to PMA treatment. PMA increased IL-6 RNA expression, binding activities of transcription factors (NF- _(k)B, AP-1) to IL-6 promoter, and IL-6 promoter activity. However, CAPE inhibited PMA-induced IL-6 mRNA expression in dose-dependent manner, and also inhibited the increased binding activities of transcription factors to IL-6 promoter and IL-6 promoter activity. These results suggest that CAPE might regulate PKC-mediated IL-6 expression and inflammatory reactions in RA.
Hur, Gang-Min,Hwang, Yin-Bang,Lee, Jae-Heun,Bae, So-Hyun,Park, Ji-Sun,Lee, Choong-Jae,Seok, Jeong-Ho 충남대학교 암공동연구소 2005 암공동연구소 업적집 Vol.4 No.
To gain insight on the role of pro-inflammatory cytokines in the pathogenesis and treatment of rheumatoid arthritis (RA), the phorbol 12-myristate 13-acetate (PMA)-induced IL-6 gene expression and the effect of caffeic acid phenethyl ester (CAPE) on the PMA-induced IL-6 gene expression were investigated in human fibroblast-like synoviocytes (FLSs). Synovial tissue samples were obtained from rheumatoid arthritis patients, and FLSs were isolated. The cells were stimulated with PMA (100 nM) for 6 hrs to induce IL-6 gene. The cells were pretreated with CAPE (20, 50, 100 μM) prior to PMA treatment. PMA increased IL-6 RNA expression, binding activities of transcription factors (NF- _(k)B, AP-1) to IL-6 promoter, and IL-6 promoter activity. However, CAPE inhibited PMA-induced IL-6 mRNA expression in dose-dependent manner, and also inhibited the increased binding activities of transcription factors to IL-6 promoter and IL-6 promoter activity. These results suggest that CAPE might regulate PKC-mediated IL-6 expression and inflammatory reactions in RA.
한국인 전반적 급진성 치주염 환자에서 IL-6 유전자 다변성에 관한 연구
방선정,김일신,김옥수,김영준,정현주,Bang, Sun-Jung,Kim, Il-Shin,Kim, Ok-Su,Kim, Young-Jun,Chung, Hyun-Ju 대한치주과학회 2008 Journal of Periodontal & Implant Science Vol.38 No.4
Purpose: The purpose of this study was to investigate the association of generalized aggressive periodontitis with IL-6 promoter gene single nucleotide polymorphisms(SNP). Material and Methods: The study population consisted of 52 generalized aggressive periodontitis patients(GAP) and 30 periodontally healthy control subjects, who were systemically healthy non-smokers. Genomic DNA was obtained from buccal swab. The IL-6 promotor SNP at the positions of -597, -572, and -174 were genotyped by amplifying the polymorphic region using polymerase chain reaction(PCR), restriction enzyme digestion and gel electrophoresis. Result: The genotype distributions for G/G, G/A and A/A genotypes of IL-6 -597 were 30.8%, 40.4%, and 28.8% in the GAP group and 53.3%, 40%, and 6.7% in the control group and were statistically different between 2 groups(p<0.05). Allele 2 frequency of IL-6 -597 were significantly higher in the GAP group than the control group(p<0.01). At the position of IL-6 -572, the distribution for C/C, C/G and G/G genotypes were 23.1%, 55.8% and 21.2% in the GAP group and 20%, 33.3%, and 46.7% in the control group. In female subjects, the genotype distribution were significantly different between 2 groups(p<0.01). In male subjects, allele 2 frequency of IL-6-572 was significantly lower in the GAP group than the control group(p<0.05). The genotype distribution of IL-6 -174 in the GAP group were 96.2%, 3.8% for G/G, G/C genotypes whereas only the G/G genotype was detected in the control group. Conclusion: In conclusion, significant associations were found in IL-6 gene promoter(-597, -572) polymorphisms and generalized aggressive periodontitis. Further cohort study will be necessary in larger population.