항정신병약물 악성증후군에서 말초신경병증을 보인 환자 1례

서유나,강철중 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25

드라이브 인 라이프스타일에 따른 주유소 공간의 전략적 재구성

서유나 경기대학교 건축전문대학원 2006 경기대학교 건축대학원 논문집 Vol.10 No.-

도시는 시간의 흐름에 따라 멈춤 없이 변화하고, 변화의 흔적들은 누적되어 가고 있다. 누적된 도시는 지역마다 언제나 거의 같은 패턴으로 나타나는 공간들을 가지고 있다. 그 공간들은 버스정류장, 지하철역, 주유소, 편의점, 고가도로 하부, 주차장 등으로 표현되고 있다. 도시의 패턴으로 나타나는 공간들은 독립적인 존재가치 보다는 네트워크 공간으로서의 가능성을 갖고 있기 때문에 도시의 변화와 사용자들의 드라이브 인 라이프스타일에 따라 재조직 되면서 도시의 모습을 새롭게 변화시킬 것이다. 본 논문에서는 에너지원의 변화와 사용자들의 드라이브 인 라이프스타일이 새롭게 변화되어 도시의 패턴으로 작용하고 있는 공간 중 주유소 공간을 재구성한다. 현재의 주유소는 사람들에게 생활의 일부분이 되어 모든 생활과 관련한 편의를 제공하는 장소로, 즉 라이프 스테이션으로 자리 잡아가고 있으나 향후 에너지 공급과 수요의 변화에 따라 기존의 주유소 공간의 역할이 변화될 것으로 예상된다. 네트워크 공간으로서의 가능성을 가진 주유소 공간은 도시의 모습을 새롭게 바꿀 수 있는 잠재력을 가지고 있다는 것을 전제로 하여 드라이브 인 라이프스타일의 소비를 위한 공간이 되기를 지향한다. 본 논문의 과정은 · 주유소의 발전과정, 분포, 공간구성, 유외사업의 현황 등을 분석하여 현재 주유소공간이 가지고 있는 문제점을 제시한다. · 화석연료의 고갈과 환경오염 문제를 저감시키기 위한 대체에너지로서 수소에너지에 대한 전망을 예측하고, 수소에너지가 주 에너지원으로 사용된다고 가정하여 설계에 적용시킨다. · 공간 사용자들의 라이프스타일을 세대별 특징과 광고를 통해 분석하고, 텔레커뮤니케이션의 발달로 인한 정보전달의 가능성을 전제로 하여 설계에 적용시킨다. · 드라이브 인 라이프스타일이 공간조직에 미치는 영향을 바탕으로 건축적 요소들을 변형시키고, 경제성과 브랜드 스페이스의 가치를 고려하여 플러그인 방식으로서의 탱그램 퍼즐게임을 적용하여 유니트들을 플러그 인/아웃시킴으로서 공간을 시뮬레이션 한다. · 시뮬레이션 된 결과물로 도시를 읽어본다. Cities keep changing as time goes on, and the traces of the changes are being accumulated. Those cities include some specific spaces that almost always appear as the same pattern. Those spaces are the bus station, subway station, gas station, convenient store, parking lot, the lower part of the overpass, and so forth. Since they function as networking spaces rather than independent spaces, the spaces as the patterns of the city would change the appearance of the city with being reconstructed by the changes of the city and the drive-in lifestyle of the users. This thesis is reconstructing the space of the gas station where the change of energy source and the users' new drive-in lifestyle become a new pattern of the city. In these days, the gas station becomes a part of people's lives so that it becomes a life station where people could be serviced of all kinds of needs, but it is predicted that the space of the gas station would play another role followed by changes of future energy supply and demand. The gas station space intends to be a space for consuming the drive-in lifestyle, under the premise that the space of a gas station has potential of changing the city figure as well as possibility of networking space. The processes of this thesis are as follows: · Suggesting issues which the present space of the gas station has by analyzing the gas station's developing process, distribution, space construction, status of non-petroleum service, and so on. · Applying it to the plan the assumption that the hydrogen energy is used as a main source of energy, by forecasting the prospects for the hydrogen energy as a kind of alternative energy, which reduce the issues of exhausted fossil fuel and environmental contamination. · Analyzing the space users' lifestyle via the characteristics of each generation and advertisements, and applying it to the plan under the possibility of information conveyance based on the development of telecommunication. · Transforming the architectural elements based on how drive-in lifestyle has influence upon the space organization. Also, simulating the space by considering economical efficiency and value of the brand space, applying Tangram puzzle game as a way of plug-in, and making units plug-in and-out. · Reading the city by means of the simulated outcome.

남성화 암쥐의 모리스 수중 미로 수행력 : 성에 따른 공간기억력과 테스토르테론 효과의 차이

신성현,박제민,정우영,박문일,서유나,김성곤,강철중 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.5

Objectives : It is widely accepted that gonadal steroids are involved in organization and activation of sexual dimorphism in the brain areas related with spatial leaming. This study aimed to test the following hypothesis : 1) spatial memory is affected by sex, 2) this difference is related with brain differentiation by exposure to testosterone in the neonatal period, and/or 3) the sexual difference of spatial memory is the result of the interaction between organization of sexual dimorphism in the brain and the circulating testosterone after growing up. Methods : Testosterone enanthate 100 ㎍, ('androgenized') orvehicle ('control') was randomly administered to 28 female and 25 male rat pups within 24 hour of birth. The escape latencies (EL) in Morris water maze were measured in 15 and 16 week of age with vehicle ('baseline') and with 10 μmol/kg of testosterone ('with testosterone'), respectively. Each session Consis-ted of 10 trials, and means of the last 5 trials were analyzed. Results : The baseline EL of the males were significantly shorter than females regardless ofneonatal exposure to testosterone. With testosterone, EL of the androgenized females were significantly shorter than control females and comparable to those of males. Conclusion : Neonatal exposure to testosterone in female rats may develop a tesosterone-dependent male-like spatial leaming system.

Molecular Characterization of Aminopeptidase N Genes from Spodoptera exigua with Susceptibility to a Bacillus thuringiensis

Na Young Jin,Mi Jin Jeon,You Kyoung Lee,San Na Oh,Mi Ja Seo,Young Nam Youn,Yong Man Yu 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04

Bacillus thuringiensis (Bt) is a gram-positive bacterium that produces parasporal crystal proteins known as endotoxins or Cry proteins. The Cry protoxins are then cleaved by insect midgut proteinases to form active Bt toxins. The activated Cry protein then binds to specific receptors at the midgut epithelium. Cadherin-like and aminopeptidase N (APN) proteins are involved in Bt toxin binding by interacting sequentially with different toxin structures. Aminopeptidase N (APNs) from several insect species have been shown to be putative receptors for these toxins. We have characterized four different midgut APNs(APN1, APN2, APN3, APN4) cDNAs from S. exigua. Forward primers and reverse primers for confirmation of four different midgut APNs were designed based on their sequences cloned from the cDNA libraries. Quantitative RT-PCR procedures includes 42℃ for 20min (cDNA synthesis), 99℃ for 5min, and 35 cycles (94℃ for 1min, and 60℃ for 50 s) for collection. Four aminopeptidase N isoforms were confirmed with qRT-PCR. Sequence analysis was performed by BlastX search the National Center for Biotechnology Information(NCBI) nucleotide. Furthermore, double-stranded RNAs(dsRNAs) were synthesized. DsRNAs were determined for bioassay.

Cell death induction and intracellular vesicle formation in human colorectal cancer cells treated with Δ9-Tetrahydrocannabinol

Hwang Yu-Na,Kwon In-Seo,Park Ju-Hee,Na Han-Heom,Kwon Tae-Hyung,Park Jin-Sung,Kim Keun-Cheol 한국유전학회 2023 Genes & Genomics Vol.45 No.12

Background Δ9-Tetrahydrocannabinol (Δ9-THC) is a principal psychoactive extract of Cannabis sativa and has been traditionally used as palliative medicine for neuropathic pain. Cannabidiol (CBD), an extract of hemp species, has recently attracted increased attention as a cancer treatment, but Δ9-THC is also requiring explored pharmacological application. Objective This study evaluated the pharmacological effects of Δ9-THC in two human colorectal cancer cell lines. We investigated whether Δ9-THC treatment induces cell death in human colorectal cancer cells. Methods We performed an MTT assay to determine the pharmacological concentration of Δ9-THC. Annxein V and Western blot analysis confirmed that Δ9-THC induced apoptosis in colorectal cancer cells. Metabolic activity was evaluated using MitoTracker staining and ATP determination. We investigated vesicle formation by Δ9-THC treatment using GW9662, known as a PPARγ inhibitor. Results The MTT assay showed that treatment with 40 μM Δ9-THC and above inhibited the proliferation of colorectal cancer cells. Multiple intracytoplasmic vesicles were detected upon microscopic observation, and fluorescence-activated cell sorting analysis showed cell death via G1 arrest. Δ9-THC treatment increased the expression of cell death marker proteins, including p53, cleaved PARP-1, RIP1, and RIP3, suggesting that Δ9-THC induced the death of colorectal cancer cells. Δ9-THC treatment also reduced ATP production via changes in Bax and Bcl-2. Δ9-THC regulated intracytoplasmic vesicle formation by modulating the expression of PPARγ and clathrin, adding that antiproliferative activity of Δ9-THC was also affected. Conclusion In conclusion, Δ9-THC regulated two functional mechanisms, intracellular vesicle formation and cell death. These findings can help to determine how cannabinoids can be used most effectively to improve the efficacy of cancer treatment.

일 대학병원에서의 알코올 의존 환자의 외래 추적시 12개월 추적률

서유나(Yu-Na Seo),정희정(Hee-Jeong Jeong),김성곤(Sung-Gon Kim),신성현(Sung-Hyun Shin),위승범(Seung-Bum Wi),변원탄(Won-Tan Byun),박선희(Sunhee Park) 한국중독정신의학회 2010 중독정신의학 Vol.14 No.1

Alcohol dependence has high prevalence and recurrence rates. To reduce these recurrence rates, alcohol dependent patients must receive continuous follow-up. However, our country has rarely studied follow-up rates for these patients. Therefore, in this study, we retrospectively investigated 3-month and 12-month ambulant follow-up rates by reviewing the hospital charts of alcohol-de-pendent patients who had received treatment in a university hospital from 2001 to 2005. The results were as follows:1) During the 5 year period, 26.6% of subjects did not make a second hospital visit in the 3 months after their first visit, and statistical analyses of alcohol-dependent patients’ follow-up rates excluded them. The patient group that was ambulant for their first psychiatric examination (outpatients) showed significantly lower rates of incomplete hospital revisiting than did the hospitalized patient group (Inpatient). 2) Regarding the final subjects, the 3-month follow-up rates was 47.9%, and the 12-month follow-up rate was 12.1%. We found no differences between the outpatient and the inpatient groups in terms of the follow-up rates. In addition, com-pared to patients admitted by psychiatric residents, patients admitted by psychiatric specialists did not show significantly lower follow-up rates. This study is the first in our country to investigate the 12-month follow-up rates for both inpatients and outpatients in this population. This study shows the need for a prospective clinical study with more participants.

Leptin-Evoked Catecholamine Release Through Activation of Protein Kinase and Its Receptors in the Rat Adrenal Medulla

Ki-Soo Kim · Yu-Seok Seo · Gwang-Moon Na · Duck-Mi Na · Hye-Gyeong Shin · Dong-Yoon Lim 조선대학교 의학연구원 2006 The Medical Journal of Chosun University Vol.31 No.1

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A multicenter study of entecavir <i>vs.</i> tenofovir on prognosis of treatment-naïve chronic hepatitis B in South Korea

Kim, Seung Up,Seo, Yeon Seok,Lee, Han Ah,Kim, Mi Na,Lee, Yu Rim,Lee, Hye Won,Park, Jun Yong,Kim, Do Young,Ahn, Sang Hoon,Han, Kwang-Hyub,Hwang, Seong Gyu,Rim, Kyu Sung,Um, Soon Ho,Tak, Won Young,Kweon Elsevier 2019 Journal of hepatology Vol.71 No.3

<P><B>Background & Aims</B></P> <P>It is currently unclear which antiviral agent, entecavir (ETV) or tenofovir disoproxil fumarate (TDF), is superior for improving prognosis in patients with chronic hepatitis B (CHB). Here, we assessed the ability of these 2 antivirals to prevent liver-disease progression in treatment-naïve patients with CHB.</P> <P><B>Methods</B></P> <P>From 2012 to 2014, treatment-naïve patients with CHB who received ETV or TDF as a first-line antiviral agent were recruited from 4 academic teaching hospitals. Patients with decompensated cirrhosis or hepatocellular carcinoma (HCC) at enrollment were excluded. Cumulative probabilities of HCC and death or orthotopic liver transplant (OLT) were assessed.</P> <P><B>Results</B></P> <P>In total, 2,897 patients (1,484 and 1,413 in the ETV and TDF groups, respectively) were recruited. The annual HCC incidence was not statistically different between the ETV and TDF groups (1.92 <I>vs</I>. 1.69 per 100 person-years [PY], respectively; adjusted hazard ratio [HR] 0.975 [<I>p</I> = 0.852] by multivariate analysis). Propensity score (PS)-matched and inverse probability of treatment weighting (ITPW) analyses yielded similar patterns of results (HR 1.021 [<I>p</I> = 0.884] and 0.998 [<I>p</I> = 0.988], respectively). The annual incidence of death or OLT was not statistically different between the ETV and TDF groups (0.52 <I>vs</I>. 0.53 per 100 PY, respectively; adjusted HR 1.202 [<I>p</I> = 0.451]). PS-matched and ITPW analyses yielded similar patterns of results (HR 1.248 [<I>p</I> = 0.385] and 1.239 [<I>p</I> = 0.360], respectively). These findings were consistently reproduced in patients with compensated cirrhosis (all <I>p</I> >0.05).</P> <P><B>Conclusions</B></P> <P>The overall prognosis in terms of HCC and death or OLT was not statistically different between the ETV and TDF groups. Further studies are needed to validate our results.</P> <P><B>Lay summary</B></P> <P>It is currently unclear which antiviral agent, entecavir or tenofovir disoproxil fumarate, is superior for improving prognosis in patients with chronic hepatitis B virus infection. In this analysis we found that there was no difference in terms of overall prognosis, including risk of hepatocellular carcinoma, death, or the need for a liver transplant, in patients receiving either antiviral.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The hepatocellular carcinoma risk was not statistically different between the ETV and TDF groups. </LI> <LI> The death or liver transplant risk was not statistically different between the 2 groups. </LI> <LI> These results were consistently reproduced after adjusting for confounding variables. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

cDNA Library Construction of Aphis gossypii Using Gateway Cloning System

Hye-Ri Kwon,Jung-Kyu Kim,Na-Yeon Ko,Yu-Bin Jung,Chan-yeong Kang,Tae-Hee Ryu,Mi-Ja Seo,Hyoun-Sub Lim,Yong-Man Yu,Young-Nam Youn 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10

Aphis gossypii was widely distributed throughout the tropical, subtropical and temperate zone. The chemical control of A. gossypii is becoming problem because it was rapidly appeared resistance expression to chemicals. We will attempt to resolve the this problem using RNAi technique. Besides, RNAi technology can be helpful to study the target genes of A. gossypii. In this study we produce cDNA library construction using gateway cloning system for selecting target gene in order to control of A. gossypii using RNAi. As a result, the 100-400bp of insert size, which is appropriate for RNAi was confirmed. Most of insert gene is associated with A. gossypii, after that insert sequence was compared with DNA databases and EST databases using NCBI blast search. Consequentially, A. gossypii of cDNA library with the titer of 3.15x105 clones were completed. And we will perform the LR recombination to transfer cDNA library into TRV2 (tobacco rattle virus) vector with att site. Then, after performing transformation using Agrobacterium tumefaciens (GV 2260), we inoculated to cucumber with A. tumefaciens. An insecticidal effect or a repellent activity against A. gossypii by changing behavior in transgenic cucumber plants were conformed. Also, the selecting target gene in order to control A. gossypii using RNAi may be provided.

간호대학생의 COVID-19에 대한 지식과 위험 인식이 COVID-19 예방행위에 미치는 영향

이혜경,강나경,김서현,김명서,남유나,노수현,이현동,정다움,주소현,최서린,Lee, Hyea-Kyung,Kang, Na-Gyeung,Kim, Seo-Hyun,Kim, Myeong-Seo,Nam, Yu-Na,Noh, Su-Hyeon,Lee, Hyeon-Dong,Jung, Da-Um,Joo, So-Hyeon,Choi, Seo-Rin 대한산업경영학회 2021 산업융합연구 Vol.19 No.5

The present study attempted to identify how the knowledge and risk perception of nursing students regarding COVID-19 impact COVID-19 preventive behaviors. This study collected data from May 1st to September 20th, 2021, using a self-reported questionnaire targeting 184 nursing students. The collected data were analyzed using Independent t-test, One-way ANOVA, and Multiple regression. The research results indicated that the knowledge(r=.15, p=.019) and risk perception(r=.13, p=.036) of nursing students regarding COVID-19 had a positive correlation with COVID-19 preventive behaviors. The factor that impacted the COVID-19 preventive behaviors of the subjects was COVID-19 knowledge, which had an explanatory power of 4%. Therefore, it is necessary to develop an educational intervention program that enables nursing students to obtain the correct knowledge regarding COVID-19 and improves preventive behaviors. 본 연구는 간호대학생의 COVID-19에 대한 지식과 위험인식이 COVID-19 예방행위에 미치는 영향을 파악하고자 시도되었다. 본 연구는 간호학과 학생 184명을 대상으로 자가 보고 설문지를 이용하여 2021년 5월 1일부터 9월 20일까지 자료를 수집하였다. 수집된 자료는 Independent t-test, One-way ANOVA, Multiple regression을 이용하여 분석하였다. 연구결과, 간호대학생의 COVID-19에 대한 지식(r=.15, p=.019), 위험인식(r=.13, p=.036)은 COVID-19 예방행위와 양의 상관관계가 있는 것으로 나타났다. 대상자의 COVID-19 예방행위에 영향을 미치는 요인은 COVID-19 지식이었으며 설명력은 4%였다. 따라서 간호대학생의 COVID-19에 올바른 지식을 습득하도록 하고 예방행위를 증진시키기 위한 교육중재 프로그램 개발이 필요하다.