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차용준,박지영,장성민,김훈,김소정 창원대학교 생활과학연구소 1998 생활과학연구 Vol.- No.2
Volatile flavor components in salt-fermented anchovy sauce were analyzed by simultaneous steam distillation-solvent extraction/gas chromatography/mass spectormetry. Ninety-eight compounds were detected in sample. These compounds were composed mainly of aldehydes(13). ketones(6), alcohols(16), aromatic compounds(21), N-containing compounds(7), esters(5), S-containing compounds(5) and miscellaneous compounds(25). Among these, 3-(methythio)propanal, 2-ethyl-3.5-dimethylpyrazine, dimethyl disulfide and dimethyl trisulfide having high odor values were supposed to be playing to be a major role in the overall aroma of salt-fermented anchovy sauce.
Newcastle Disease Virus의 Fusion 단백 유전자의 제한효소분절형태(RELP)의 특이성
장경수,김지영,김석,김태용,송영환,허지연,김현수,송희종,전무형 충남대학교 수의과대학 동물의과학연구소 2001 動物醫科學硏究誌 Vol.9 No.-
To evaluate the specificity of restriction fragment length polymorphism (RFLP) of Newcastle disease virus (NDV) fusion (F) protein gene for various NDV strains. F protein genes of NDV CBP-1 isolate, LaSota, B1 and Kyojungwon (KJW) were amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) and RFLP were examined. In RT-PCR using F primer sets for 1.7 kb F gene, the amplified products were observed in CBP-1, LaSota and B1 except KJW. However, F2 primer set for 521 bp yielded the amplified bands with all strains. The amplified F genes (1.7 kbp) were treatd with 15 different restriction enzymes to analyze RFLP and the cleavage patterns. CBP-1 showed various cleavage patterns with 8 enzymes, LaSota, with 11 enzymes and B1, with 11 enzymes. According to the cleavage patterns, the physical maps were constructed, and it was found that CBP-1, LaSota and B1 strains had specific cleavage sites with HinfI, XbaI and XhoI. These findings suggest that RFLP are worth applying in the diagnosis and molecular epidemiological studies of Newcastle disease.
Profiling Transcripts by EST from Vitis coignetiae against Anthracnose Infection
Youn Jung Choi,Soon Young Ahn,Seung Hui Kim,Youn Young Hur,Hae Keun Yun 한국국제농업개발학회 2011 韓國國際農業開發學會誌 Vol.23 No.4
머루(Vitis coignetiae)는 국내에 자생하는 야생종 포도의 한 종류로서, 포도 새눈무늬병에 저항성이다. 내병성 포도 육종에 활용할 유용한 육종소재를 개발하고자 머루의 잎과 과실로부터 cDNA libraray를 제작하였다. 머루의 cDNA library의 총 5,760개의 EST 클론을 분석하여 676개의 contig와 2,306개의 singleton을 분리하여 총 2,982개의 unigene을 분리하였다. NCBI 데이터베이스의 BLAST를 통한 상동성을 검색한 결과, 2,241개의 클론이 기능이 알려진 유전자이었고, 그 중에서 1,442개는 생물학적인 대사에 관여하고 836개는 세포구성물과 관련이 있었다. EST와 contig의 평균 크기는 각각 702bp와 757bp이었다. 포도새눈무늬병균에 감염된 머루 cDNA library로부터 Proline-rich cell wall protein, thaumatin-like protein, class IV chitinase, and pathogenesis-related (PR) protein 10 등의 다양한 방어관련 유전자와 광합성관련유전자 및 수분스트레스 저항성 관련유전자가 많이 검출되었다. 본 연구를 통해 얻어진 머루의 EST 자료는 포도의 새로운 유전자원에 관한 연구 및 내병성 포도 신품종 육종 프로그램에 기본자료로 유용하게 활용될 것이다. Vitis coignetiae, a wild grape native to Korea, is resistant to anthracnose diseases. For screening useful resources for disease-resistant grape breeding, a cDNA library was constructed from leaves of wild grapes. A total of 2,982 unigenes containing 676 contigs and 2,306 singletons were obtained by sequencing 5,760 expressed sequence tag (EST) clones derived from the cDNA library of wild grape, V. coignetiae infected with Elsinoe ampelina causing anthracnose disease. In gene ontology analysis, 2,241 genes with molecular functions were annotated into 1,442 biological processes, and 836 cellular component genes. The average lengths of the ESTs and contigs were 702 and 757 bp, respectively. Various defense-related genes, such as putative proline-rich cell wall protein, thaumatin-like protein, class IV chitinase, and pathogenesis-related (PR) protein 10, photosynthesis-related genes, such as ribulose-bisphosphate carboxylase oxygenase small subunit, and water stress-related genes, such as abscisic stress ripening protein, were the most abundant in cDNAs from leaves of V. coignetiae infected with Elsinoe ampelina. These data could provide useful information in the genetic analysis of V. coignetiae and in the program for breeding disease-resistant grape cultivars.
F-103 Lessons learned from continued TB outbreaks in a high school
( Young Kim ),( Young Seok Lee ),( Jee Youn Oh ),( Won Jai Jung ),( Eun Joo Lee ),( Kyung Hoon Min ),( Gyu Young Hur ),( Sung Yong Lee ),( Sang Yeub Lee ),( Jae Jeong Shim ),( Eui Sook Kim ),( Yoon So 대한결핵 및 호흡기학회 2016 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.121 No.-
Objective: Contact investigation with Tuberculin Skin Test (TST) and delayed QuantiFERON testing (QFT) had been conducted against continued tuberculosis (TB) outbreaks in a high school before Korea latent TB infection (LTBI) policy establishment. We investigated the aftereffect of confirmatory QFT added to positive TST. Methods: We reviewed the outbreaks courses, and identified the TB development in relation to initial TST and QFT with the aid of Korean national claims database. Results: Continued sequential 43 active TB cases were diagnosed in a high school for one year before LTBI investigation. After contact investigation, a total of 925 contacts were divided into three groups: TST- (n=632), TST+/QFT+ (n=25), TST+/QFT- (n=257). The mean duration of follow-up was 3.9 ± 0.9 years. QFT- was more prevalent compared with QFT+ in contacts with 10mm ≤ TST <15mm (157, 61.1%) than in contacts with TST ≥15mm (100, 38.9%) among the TST positive reactors (Odds ratio 4.97, C.I: 1.80 - 14.46, P < 0.001). Of the 257 TST+/QFT- subjects, 255 received no LTBI treatment, and 7 contacts developed TB during follow-up. Among these 7 patients, four had initial TST ≥15mm, and three 10mm ≤ TST <15mm. Conclusions: The delayed contact investigation for LTBI in a high school TB outbreak brought upon the continuous successive TB outbreaks. False negative QFT performed lately among the TST positive reactors should not be considered as a criteria for LTBI treatment. Additionally, the contacts only with strong induration size of TST (≥ 15mm) should be considered for LTBI treatment.
포도 새눈무늬병균을 접종한 머루(Vitis coignetiae) cDNA Library의 EST 분석
최윤정 ( Youn Jung Choi ),안순영 ( Soon Young Ahn ),김승희 ( Seung Hui Kim ),허윤영 ( Youn Young Hur ),윤해근 ( Hae Keun Yun ) 한국국제농업개발학회 2011 韓國國際農業開發學會誌 Vol.23 No.4
Vitis coignetiae, a wild grape native to Korea, is resistant to anthracnose diseases. For screening useful resources for disease-resistant grape breeding, a cDNA library was constructed from leaves of wild grapes. A total of 2,982 unigenes containing 676 contigs and 2,306 singletons were obtained by sequencing 5,760 expressed sequence tag (EST) clones derived from the cDNA library of wild grape, V. coignetiae infected with Elsinoe ampelina causing anthracnose disease. In gene ontology analysis, 2,241 genes with molecular functions were annotated into 1,442 biological processes, and 836 cellular component genes. The average lengths of the ESTs and contigs were 702 and 757 bp, respectively. Various defense-related genes, such as putative proline-rich cell wall protein, thaumatin-like protein, class IV chitinase, and pathogenesis-related (PR) protein 10, photosynthesis-related genes, such as ribulose-bisphosphate carboxylase oxygenase small subunit, and water stress-related genes, such as abscisic stress ripening protein, were the most abundant in cDNAs from leaves of V. coignetiae infected with Elsinoe ampelina. These data could provide useful information in the genetic analysis of V. coignetiae and in the program for breeding disease-resistant grape cultivars.