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Tran Ha Thi Thanh,Dang Anh Kieu,Ly Duc Viet,Vu Hao Thi,Hoang Tuan Van,Nguyen Chinh Thi,Chu Nhu Thi,Nguyen Vinh The,Nguyen Huyen Thi,Truong Anh Duc,Pham Ngoc Thi,Dang Hoang Vu 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.10
Objective: The rapid and reliable detection of the African swine fever virus (ASFV) plays an important role in emergency control and preventive measures of ASF. Some methods have been recommended by FAO/OIE to detect ASFV in clinical samples, including real-time polymerase chain reaction (PCR). However, mismatches in primer and probe binding regions may cause a false-negative result. Here, a slight modification in probe sequence has been conducted to improve the qualification of real-time PCR based on World Organization for Animal Health (OIE) protocol for accurate detection of ASFV in field samples in Vietnam. Methods: Seven positive confirmed samples (four samples have no mismatch, and three samples contained one mutation in probe binding sites) were used to establish novel real-time PCR with slightly modified probe (Y = C or T) in comparison with original probe recommended by OIE. Results: Both real-time PCRs using the OIE-recommended probe and novel modified probe can detect ASFV in clinical samples without mismatch in probe binding site. A high correlation of cycle quantification (Cq) values was observed in which Cq values obtained from both probes arranged from 22 to 25, suggesting that modified probe sequence does not impede the qualification of real-time PCR to detect ASFV in clinical samples. However, the samples with one mutation in probe binding sites were ASFV negative with OIE recommended probe but positive with our modified probe (Cq value ranked between 33.12-35.78). Conclusion: We demonstrated for the first time that a mismatch in probe binding regions caused a false negative result by OIE recommended real-time PCR, and a slightly modified probe is required to enhance the sensitivity and obtain an ASF accurate diagnosis in field samples in Vietnam.
Ha Thi Thanh Tran,Duc Anh Truong,Viet Duc Ly,Hao Thi Vu,Tuan Van Hoang,Chinh Thi Nguyen,Nhu Thi Chu,Vinh The Nguyen,Duyen Thuy Nguyen,Kohtaroh Miyazawa,Takehiro Kokuho,Hoang Vu Dang 대한백신학회 2020 Clinical and Experimental Vaccine Research Vol.9 No.1
Purpose: To date, many kinds of classical swine fever (CSF) vaccines have been developed to protect against this disease. However, the efficacy of these vaccines to protect the pig against field CSF strains needs to be considered, based on circulating strains of classical swine fever virus (CSFV). Materials and Methods: Recombinant E2-CSFV protein produced by baculovirus/insect cell system was analyzed by western blots and immunoperoxidase monolayer assay. The effect of CSFV-E2 subunit vaccines was evaluated in experimental pigs with three genotypes of CSFV challenge. Anti-E2 specific and neutralizing antibodies in experimental pigs were analyzed by blocking enzyme-linked immunosorbent assay and neutralization peroxidize-linked assay. Results: The data showed that CSFV VN91-E2 subunit vaccine provided clinical protection in pigs against three different genotypes of CSFV without noticeable clinical signs, symptoms, and mortality. In addition, no CSFV was isolated from the spleen of the vaccinated pigs. However, the unvaccinated pigs exhibited high clinical scores and the successful virus isolation from spleen. These results showed that the E2-specific and neutralizing antibodies induced by VN91-E2 antigen appeared at day 24 after first boost and a significant increase was observed at day 28 (p<0.01). This response reached a peak at day 35 and continued until day 63 when compared to controls. Importantly, VN91-E2 induced E2-specific and neutralizing antibodies protected experimental pigs against high virulence of CSFVs circulating in Vietnam, including genotype 1.1, 2.1, and 2.2. Conclusion: These findings also suggested that CSFV VN91-E2 subunit vaccine could be a promising vaccine candidate for the control and prevention of CSFV in Vietnam.
( Ly Thi Minh Pham ),( Wann Yih Wu ),( Hong Nga Do ),( Tuan Minh Phung ) 세계문화관광학회 2015 International Journal of Culture and tourism Resea Vol.8 No.1
The purpose of this study is to examine how brand equity, from a customer point of view, influences quick-service restaurant revisit intention. The authors propose a conceptual framework in which three dimensions of brand equity including brand associations combined with brand awareness, perceived quality, brand loyalty and perceived value are related to revisit intention. Data from 570 customers who have visited 4 quick-service restaurants in Ho Chi Minh City were used for the structural equation modeling (SEM) analysis. The results show that strong brand equity is significantly correlated with revisit intention. Additionally, the effect of brand equity on revisit intention was mediated by perceived value, among others. Overall, this study emphasizes the importance of perceived value in lodging customers’ mind. Finally, managerial implications are presented based on the study results.
N-recognins UBR1 and UBR2 as central ER stress sensors in mammals
Ly Thi Huong Luu Le,Seoyoung Park,Jung Hoon Lee,Yun Kyung Kim,Min Jae Lee Korean Society for Molecular and Cellular Biology 2024 Molecules and cells Vol.47 No.1
In eukaryotes, a primary protein quality control (PQC) process involves the destruction of conformationally misfolded proteins through the ubiquitin-proteasome system. Because approximately one-third of eukaryotic proteomes fold and assemble within the endoplasmic reticulum (ER) before being sent to their destinations, the ER plays a crucial role in PQC. The specific functions and biochemical roles of several E3 ubiquitin ligases involved in ER-associated degradation in mammals, on the other hand, are mainly unknown. We identified 2 E3 ligases, ubiquitin protein ligase E3 component N-recognin 1 (UBR1) and ubiquitin protein ligase E3 component N-recognin 2 (UBR2), which are the key N-recognins in the N-degron pathway and participate in the ER stress response in mammalian cells by modulating their stability. Cells lacking UBR1 and UBR2 are hypersensitive to ER stress-induced apoptosis. Under normal circumstances, these proteins are polyubiquitinated through Lys48-specific linkages and are then degraded by the 26S proteasome. In contrast, when cells are subjected to ER stress, UBR1 and UBR2 exhibit greater stability, potentially as a cellular adaptive response to stressful conditions. Although the precise mechanisms underlying these findings require further investigation, our findings show that cytoplasmic UBR1 and UBR2 have anti-ER stress activities and contribute to global PQC in mammals. These data also reveal an additional level of complexity within the mammalian ER-associated degradation system, implicating potential involvement of the N-degron pathway.
Comparative Efficiency of Container Ports in Vietnam and Korea
Ly, Pham Thi Hoai,Ahn, Ki-Myung,Ryoo, Dong-Keun Korean Institute of Navigation and Port Research 2018 한국항해항만학회지 Vol.42 No.2
Currently, there are very few papers that have examined the efficiency of Vietnamese ports. Of those that have, some of them take into account only a few container ports in Northern Vietnam. Others focus on comparing all types of ports in Vietnam with regard to the differences in efficiency scores when applying different methods to evaluate efficiency. However, there is no paper that specifically analyzes the efficiency of Vietnamese container ports throughout the country or that compares Vietnamese container ports with those of other countries in terms of efficiency. In order to provide more accurate and interesting information for container port authorities, in this study, 21 major Vietnamese container ports and seven major Korean container ports are selected and examined with regard to their levels of efficiency in 2016 using data envelopment analysis (DEA). This paper applies both constant return to scale (CRS) and variable return to scale (VRS) to explore the differences among overall technical efficiency, pure technical efficiency, and scale efficiency of the selected container ports. This study also indicate slacks and sets projections for inefficient ports.
Ly Thi Minh Huynh,이수현,윤상운 대한화학회 2019 Bulletin of the Korean Chemical Society Vol.40 No.8
We provide practical information about the basic properties of SAMs to prepare and modify NPoM systems.
Thi Ly Vu,Trung Dung Do,Cheng-Bin Jin,Shengzhe Li,Van Huan Nguyen,Hakil Kim,Chongho Lee 한국정보과학회 2015 Journal of Computing Science and Engineering Vol.9 No.1
Human action recognition has become an important research topic in computer vision area recently due to many applications in the real world, such as video surveillance, video retrieval, video analysis, and human-computer interaction. The goal of this paper is to evaluate descriptors which have recently been used in action recognition, namely Histogram of Oriented Gradient (HOG) and Histogram of Optical Flow (HOF). This paper also proposes new descriptors to represent the change of points within each part of a human body, caused by actions named as Histogram of Changing Points (HCP) and so-called Average Speed (AS) which measures the average speed of actions. The descriptors are combined to build a strong descriptor to represent human actions by modeling the information about appearance, local motion, and changes on each part of the body, as well as motion speed. The effectiveness of these new descriptors is evaluated in the experiments on KTH and Hollywood datasets.
Pervaporative separation of butanol using a composite PDMS/PEI hollow fiber membrane
Ly Thi Phi Trinh,Young Ju Lee,배현종,이홍주 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.5
In the study, the separation and purification of butanol was carried out using the composite hollow fiber membrane having the active layer of polydimethylsiloxane (PDMS) on the macroporous support of polyetherimide (PEI). The pervaporation results with the initial butanol concentration showed a trade-off between flux and separation factor. However, both the flux and the separation factor increased as the operating temperature increased. The pervaporation results showed the butanol flux and the separation factor were higher than those of the reported results. In this study, butanol was concentrated by the pervaporation as a feasibility study for the biofuel applications. 2013 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
Pervaporative separation of bioethanol produced from the fermentation of waste newspaper
Ly Thi Phi Trinh,이홍주,조은진,배현종 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.6
In this study, a hydrophobic polymeric polydimethylsiloxane (PDMS) membrane was used for the pervaporative separation of bioethanol produced from fermentation of lignocellulosic biomass (waste newspaper) and glucose. As a preliminary study, the pervaporation permeation performance showed strong dependence on feed concentration and temperature. The pervaporation of bioethanol produced by the fermentation of waste newspaper by Saccharomyces cerevisiae decreased process performance. However, the process performance was restored reversibly by water cleaning. The pervaporative separation of bioethanol from the fermentation of waste newspaper was carried out without any significant decreasing process performance in the study.
Ly Thi Phi Trinh,이영주,박찬송,배현종 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.69 No.-
Acidified [Bmim]Cl solution showed a great efficacy for the pretreatment of softwood, hardwood, rice straw, and sugarcane bagasse. The pretreatment resulted in 29 and 20-fold increase in cellulose digestibility of hardwood and softwood, respectively, and most cellulose in rice straw and sugarcane bagasse was converted into glucose. Fermentation of biomass hydrolysates produced 21.86–29.56 g/L ethanol, thereby achieving ethanol yields of 0.49–0.51 g/g. Ethanol was concentrated up to 79.8% from a fermentation broth containing 2.4% ethanol using four consecutive pervaporations. This work demonstrates the viability of biofuel production from diverse feedstocks and the feasibility of ethanol recovery using pervaporative separation.