지단백과 알부민 및 이들의 변형물질이 메산지움세포 증식에 미치는 영향

손일석,이태원,박재경,김희진,조병수,임천규,김명재 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2

목 적 : 혈청 지단백과 알부민은 그 자체로써 또는 산화나 당화 과정에 의한 변형을 거쳐 사구체 질환을 진행시킬 수 있는 것으로 알려져 있다. 그 기전은 분명하지는 않으나 메산지움세포 증식과 관련이 있는 것으로 알려져 있다. 저자들은 비교적 간단한 흡광도측정법을 이용하여 지단백과 알부민 및 그들의 변형물질들이 각각 메산지움세포 증식에 미치는 영향을 알아보고자 하였다. 방 법: 정상인의 혈청 지단백을 원심분리 하여 저밀도지단백, 고밀도지단백, 초저밀도지단백을얻고, 산화 저밀도지단백, 당화 저밀도지단백, 그리고 혈청 알부민과 당화 알부민을 각각 농도를 달리하여 배양 중인 인간 메산지움세포에 가한 후 흡광도를 측정함으로써 메산지움세포의 증식정도를 알아보았다. 양성 대조군으로 interleukin-1β를 이용하였다. 결 과: 저밀도지단백은 농도 증가시 50 μg/mL와 100 μg/mL에서 세포의 증식을 보였으며,고밀도지단백과 초저밀도지단백은 농도에 따른 유의한 변화가 없었다. 산화 저밀도지단백은5 μg/mL에서부터 세포의 증식을 초래한 후 10 μg/mL와 25 μg/mL에서도 이를 유지하였다. 당화 저밀도지단백은 농도 증가에 따라 10 μg/mL와 100 μg/mL에서 세포의 증식을 억제하였다. 알부민은 농도 500 μg/mL에서 세포 증식을 억제하다가 고농도(1,000 μg/mL)에서는 영향이 없었으며, 당화 알부민 역시 100 μg/mL의 적은 농도에서는 억제효과를 보였으나 고농도(1,000 μg/mL)에서는 오히려 증식을 일으키는 결과를 보였다. 결 론: 저농도에서 저밀도지단백과 산화 저밀도지단백은 사람 메산지움세포의 증식을 초래하였으며, 당화 저밀도지단백은 증식을 억제하였다. 알부민은 일정농도까지 사람 메산지움세포의 증식을 억제하다가 고농도에서는 영향이 없었으며, 당화 알부민도 일정농도까지 증식 억제 효과가 있다가 고농도에서는 오히려 증식을 초래하는 양상을 보였다. Background : Modified lipoproteins may be involved in nephro- and glomerulosclerosis. Diabetic nephropathy-like lesions have also been induced in a rat model by glycated and glycoxidized albumin. In cultured rat or human mesangial cells, enhanced cell proliferation and production of mesangial matrix in response to lipoproteins and their modified forms have been demonstrated by [3H]-thymidine incorporation and cell counting assays. But these methods are relatively complex and most of them have used only one or two of the lipoprotein, albumin and their modified forms. Methods : We investigated the effects of native and modifed lipoproteins, and albumin on cultured human mesangial cell proliferation using non-radioactive colorimetric method by MTS/PMS assay. Lipoproteins added were low density lipoprotein(LDL), high density lipoprotein(HDL), very low density lipoprotein(VLDL), oxidized LDL(oxidation with copper sulfate in vitro) and glycated LDL and we also used albumin, glycated albumin, and interleukin-1β as a positive control. Results : Interleukin-1β promoted the proliferation of cultured human mesangial cells up to concentration 20 ng/mL. LDL induced the proliferation of mesangial cells in a concentration-dependent manner up to concentration 100 μg/mL. HDL and VLDL had no significant proliferative effect. Oxidized LDL caused the proliferation of mesangial cells at low concentration up to concentration 25 μg/mL. Addition of glycated LDL resulted in a concentrationdependent inhibition of mesangial cells. Albumin and glycated albumin inhibited the proliferation of mesangial cells at low concentration of 100 μg/mL, but cell growth was increased at higher concentrations. Conclusion : We demonstrated the effects of the single and modified proteins on the proliferation of cultured human mesangial cell by relatively simple colorimetric method. Results were almostly identical to those of previous studies obtained by radioactive method or cell counting assay. (Korean J Nephrol 2002;21(2):266-275)

텅스텐 산화 피막을 입힌 NO₂수정 압전 가스센서의 감응성에 관한 연구

손태원,민태엽 弘益大學校 科學技術硏究所 1995 科學技術硏究論文集 Vol.6 No.-

This experiment carried out sensing property of tungsten trioxide with QCM (quartz crystal microbalace) device. The stability, sensitivity and selectivity of dip-coated tungsten trioxide (WO₃) films gas sensing applications have been examined. In this experiment, the WO₃ film sensing properties, such as adsorption, desorption and reversivity for NO₂ gas, Measured. The WO₃ films are exposed to NO₂ gas and are found to be very sensitive to NO₂.

양이온의 층간 반응에 의한 텅스텐 산화막과 전해질 계면에서의 전기화학적 특성에 관한 수치해석

孫太源,趙成鎭 弘益大學校 科學技術硏究所 1998 科學技術硏究論文集 Vol.9 No.2

In this study, optical density for tungsten oxide film is investigated by equation of diffusion derived for cation diffusion into film. Current is calculated for one case; reversible case that reaction rate is very fast. Dimensionless parameter ξ, ratio of diffusion coefficient in electrolyte and inside of film, and ζ, include exchange current density, are defined. Optical density is calculated with distribution of cation concentration that is calculated for dimensionless parameters in film. In reversible case, concentration of interfacial of electrolyte and film is very high and it takes long time to distribute uniformly. Change of optical density is great for change of diffusion coefficient.

무시안 알칼리 아연 도금을 위한 Hull cell Test

孫太源 弘益大學校 科學技術硏究所 2005 科學技術硏究論文集 Vol.16 No.-

Noncyanide alkaline plating process on zinc was studied by analysing the process technology through efficiency test, analysis of fluid composition, and physical property research on existing oversea manufactures. Also it was compared suggested solution's standard composition with manufactures' one under plating conditions and changes of solution composition, consumption, impurity effect. It was examined electrochemical characteristics of agents such as complexing agent, conductivity reinforcing agent and stability. Zinc plating characters such as uniformity, brightness using Hull cell test. Electrochemical quality of zinc plating solution was studied by searching current density, current efficiency and cyclic voltammeter. Suggested product's plating characteristics such as agent's quantity, current density and relation with plating temperature are composed with existing cyanide plating process and oversea products through Hull cell test. Suggested product showed superior qualities in most of aspects such as uniformity, brightness, and leveling. Even comparing to cyanide alkaline plating, sample showed superiority in every aspect except plating rate. However, at high current region, brightness bounds became narrowed compare to oversea product.

공장에서의 금속 부식에 대한 문제점과 그 대책

손병청,손태원,임병오 弘益大學校 1980 弘大論叢 Vol.12 No.-

It must be obvious that the corrosion has been treated rightly and overlooked because of its long history and ever-slow taking process. Also it is difficult to find its nature and anti-corrosion scheme, since the theory of corrosion is a complex mixture of a variety of natural sciences with electrochemisty as the sheet anchor. Further, it must be again obvious that the working engineer generally has neither the time, nor the training, to comprehend the whole theory. Most companies have no corrosion engineer and simply exchange corroded parts without trying any other anti-corrosion method. Some of them have been depend upon the foreign companies associated with them. A few companies are begining to tackle their corrosion problem by the cooperation with the domestic corrosion engnieers and researchers. It has been a consequence of the complexity of corrosion nature that, over the years, an uncomplicated common-sense approach to anticorrosion methods has been developed. This anti-corrosion methods may help to enable an engineer to tackle his corrosion problems with confidence.

觸媒粒子 內에서 細孔擴散이 選擇度에 미치는 影響

孫太源,廉聖培 弘益大學校 1983 弘大論叢 Vol.15 No.2

The in fluence of pore diffusion on the yield and selectivity has been studied for a chemical reaction occuring in a catalyst particle. When a consecutive reaction (A???→R???→S) proceeds, mathematical formulation is performed by introducing Wheeler's classical work. Also the variation of the yield of intermediate product R due to conversion of reactant A was considered incase of plug flow reactor. By computations, favorable conditions to increase yield of desired product R with various values of parameters were obtained. The present results show that it is favorable when the reaction rate constant k₁is larger than k₂. And as a whole, the values of lower Thiele modulus for a nonporous catalyst and of higher effective diffusivity ratio tend to increase the yield of intermediate product.

3.5% NaCl 溶液에서 炭素鋼의 Ethylendiamine에 대한 腐蝕 抑制 Mechanism에 關한 硏究

孫太源,閔丙喆 弘益大學校 1990 弘大論叢 Vol.22 No.2

The inhibitive effect of carbon steel S.S 41 in the 3.5% NaCl solution has been studied using polarization test method. A significant retardation of corrosion rate was observed in the NaCl solution containing the inhibitor. The rate of corrosion was depended on the nature of the inhibitor and its concentration and tempratures of the environment. The results obtained from polarization test method were revealed that ethylendiamine performed a dual function in corrosion inhibition of carbon steel; one was a neutralization of the solution and the other was the adsortion of ethylendiamine on the metal surface. Using Bockris-Swinkels adsorption isotherm, the expected value of n and the heat of adsorption for ethylendiamine on carbon steel surface were estimated to 5, 57.38Kcal/mole, respectively. The activation energy in the uninhibitive and inhibitive NaCl solution were observed as a value characteristic of diffusion process.

Quartz Piezoelectric 가스센서의 특성에 관한 연구

孫太源,李炳昊,閔丙喆 弘益大學校 科學技術硏究所 1994 科學技術硏究論文集 Vol.5 No.-

A quartz piezoelectric crystal coated with Langmuir-Blodgett films of tetra-3-hexadecyl sulphamoyl copper phthalocyanine (HDSM-CuPc) was examined as a gas sensor for NO₂. HDSM-CuPc LB films were transfered to a quartz crystal microbalance (QCM) in the form of mono or Y-type multilayer. Surface pressure of HDSM-CuPc was maintained at 32 mN/m during film transferation. Frequency changes of QCM were acquired by microcomputer via PM6680 frequency counter. Response of film coated QCM to NO₂gas over a range of 100∼400 ppm NO₂and 1∼10 layers of LB films have been tested. Changes in frequency by adsorption of NO₂were increase with the number of LB layer and NO₂concentration, but response was slow.

용융탄산염 내에서의 Stainless steel의 부식특성에 관한 연구

孫太源,金俊亨,金炳天 弘益大學校 科學技術硏究所 1997 科學技術硏究論文集 Vol.8 No.-

The effect of oxidant gas on corrosion behavior of separator material for molten carbonate fuel cell, the AISI 316L stainless steel in the molten carbonates was investigated by using the electrochemical techniques. The composition of electrolyte used in this study were 62mol% Li₂CO₃ and 38mol% K₂CO₃. The concentration of oxidant gases CO₂, O₂ were varied from 5vol% to 50vol% respectively. it had been found the increase of oxidant gas concentration proportionally enhanced the corrosion rate of AlSl 316L stainlees steel. From the result, we obtained to the relation between Ecorr, Icorr and CO₂ gas partial pressure and also O₂ gas partial pressure. Therefore it is suggested Ecorr ∝ P(o₂)??, Icorr ∝ P(o₂)?? and Ecorr ∝ P(co₂)?? , Icorr ∝ P(co₂)??.

RuxIr₁xO₂/Ti mesh 촉매전극에 의한 페놀의 전기화학적 산화특성에 관한 연구

孫太源,李相雄 弘益大學校 科學基術硏究所 1999 科學技術硏究論文集 Vol.10 No.2

One of the best ways of turning the characteristics of aromatic compounds the major anti-degradable compounds, into the one of bio-degradable is electrochemical oxidation. Among the aromatic compounds chlorine group on phenol has been chosen and the probabilities of electrochemical oxidation was researched by measuring Cyclic voltammogram(CV) and Tafel polarization curve for RUx.rxO₂/Ti mesh catalytic electrode. The value of HPP using Ru/Ir 7:3 electrode was smaller and i?? was larges than using Ru/Ir 3:7 electrode. Ru/Ir 7:3 electrode was better than Ru/Ir 3:7 electrode on the electrochemical oxidation of phenol.