韓國在來山羊의 綱膜神經節細胞數와 分布에 關한 硏究

金武剛,趙聲煥,申台均 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.2

The number and distribution of retinal ganglion cells in the Korean native goats was studied with whole mount preparations stained with methylene blue and thionin. The mean area of 5 retinae is 918 mm² and the mean number of total ganglion cells was 1, 602, 000. So the mean number of ganglion cells per mm² was 1,745. The density distribution of the retinal ganglion cells showed that visual streak was located about 2mm dorsal to the optic disc, and area centralis (7,700 cells/mm') was located about 10mm temporally and 2mm dorsal to the optic disc. There was drop in density of retinal ganglion cells about the circumference of the visual streak in dorsal and ventral direction. The functional significance of the findings was also discussed.

Nicotine이 발생중인 鷄胎의 體重 成長에 미치는 영향

金武剛,金弘善,柳時潤,申台均,李永浩,趙聲煥 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

The authors studies the effects of Nicotine on the Body weight growth of the developmental chick embryo. On the sixth day of incubation, 0.41mg, 0.82mg and 1.65mg of Nicotine were injected into the chorioallantoic sac of each egg of three groups and one group was injected physiological saline solution as a control, and the eggs were broken for weighting the body weight in every other day from the twelfth day to eighteenth day of incubation. In an attempt to analyze the growth of the body weights, growth formula of Zimmerman and relative growth formula of Huxley were applied. The following results were obtained. 1. The growth of chick embryo boby weight was affected as inhibiting by the Nicotine. The inhibiting effects were in proportion to the injected Nicotine dosage. 2. The growth quantity of the body weight injected 1.65mg was almost half weight of the control group body weight. 3. Growth rate was decreased continuously with tine until 18th day in the 1.65mg injected group but it was increased in 0.41mg and 0.82mg injected group with time lapse from 12th day to 18th day, and 0.41mg injected group was more increased than 0.82mg injected group. 4. Specific growth rate decreased in the all of 0.41mg, 0.82mg and 1.65mg injected group and in the decrease degree 1.65mg and 0.82mg injected group ware severe and 0.41mg injected group was slight. 5. Growth centers of growth gradient were 12th day in the 0.41mg injected group and 18th day in the 0.82mg and 1.65mg injected groups. 6. Relative growth rate was also decreased proportionally in related with injected Nicotine dosage.

생쥐 鼻中隔 各種 粘膜上皮 및 粘膜下腺의 分布樣狀과 그 表面積에 관한 硏究

金東煥,金武剛,申台均,金弘善,柳時潤,李永浩 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.1

In order to set the distribution pattern and surface area of the mucosal epithelium and submucosal gland in the mice nasal septum. 3-month-old healthy 6 mice weighing 25gm approximately have been contributed for this research. For the mapping of the each nasal epithelium and submucosal gland in the submicroscopic level, the routine paraffin block of the mice nasal septum were cut as the 10㎛ thick at 110㎛ intervals. Consecutive 2 slides were stained with H-E and PAS and reconstructed in form of maps representing the structure in a sagittal plane. The results obtained from this research were summarized as follows. 1. The unilateral nasal surface epithelial area of the mice nasal cavity was 31.23㎟ 2. The areas of the stratified squamous epithelium, respiratory epithelium and olfactory epithelium which compose the unilateral nasal epithelium were 4.47㎟(14.3%), 12.7㎟(40.8%), and 14.02㎟(44.9%), respectively. 3. Submucosal glands in the mice nasal septum were divided into the PAS-positive, PAS-negative and Bowman's glands by the PAS staining. 4. The surface area of the PAS-positive glands in the nasal unilateral septum was 5.23㎟ which is located in the central area of the respiratory portion. 5. The surface area of the PAS-negative glands in the nasal unilateral septum was 5.05㎟ which is located above and below the PAS-positive gland. 6. The surface area of the Bowman's glands was 12.1 ㎟ which was located in the olfactory region. 7. In the vestibular region., 3 ducts of PAS-negative glands of the respiratory region are found, and below the nasal septum there is the infraseptal gland(PAS-negative).

각종 동물 설유두의 형태학적인 관찰

김홍선,김무강,김원식,이영호,신태균 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

The lingual papillae of the mouse, the rabbit, and the cat were observed by light microscope and scanning electron microscope The results were followings 1. The filiform papillae of the mouse were classified to typical filiform papillae, conical filiform papillae with 2 branched tips, and general conical filiform papillae. The outmost layers of the filiform papillae of the mouse keratinted well, middle layer of the anterior portion of the papillae appeaered a lot of small basophilic granules. The fungiform papillae existed between the filiform papillae with the peripheral folds of epithelia. A vallate papilla existed on the posterior portion of conical filiform papillae and their shape was lenticuiar. 2. The filiform papillae of the rabbit revealed various type (5 types). Keratin layer of the papillae existed vertical to long axis of the papillae on the posterior portion of the papillae. Stratified squamous epithelium on the anterior portion of the papillae existed with a few keratin layers and the intercellular materials of strong PAS positive reaction appeared between the these cells. The fungiform papillae of the rabbit existed between the filiform papillae and had a few peripheral folds. The foliate papillae of the rabbit existed on the lateral portion of tongue root, consisted of the paralleled folds. Taste buds located in the mid-lateral portion of the papillae concentrically. 3. The filiform papillae on the anterior portion of the cat tongue shaped anterior and posterior projection, the filiform palillae on the middle portion had a projections of columnar shape, which papillae surface appeared as onion surface. The conical filiform papillae on the posterior portion had many folds on the lower portion of the papillae. The fungiform papillae of the cat existed between the filiforr papillae with a columnar shaped projectionand proturded from the surface. The vallate papillae of the cat existed between the conical filiform papillae and their shape was typical form of vallate papillae.

오리 網膜에 存在하는 神經節細胞의 數와 分布에 關한 硏究

姜翰秀,金武剛,申台均,金弘善,柳時潤,李永浩 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.1

The number and distribution of the retinal ganglion cells in the 1-week-old and 7-week-old ducks were determined from whole flat mounted preparations stained with methylene blue and thionin. The results were summarized as follows. 1. In the 1-week-old and 7-week-old ducks, the areas of the retinas were 269.4㎟ and 357.2㎟, respectively, and the total cell numbers 1,742,800 and 1,808,800, respectively. 2. The ganglion cells based upon soma diameter were classified into 3 groups such as small (below 10㎛), medium (10-20㎛) and large (above 20㎛) ganglion cells. In 1-week-old duck, the relative frequencies of these 3 groups were 91.0%, 8.5% and 0.5%, respectively, and in 7-week-old duck, 83.7% 14.7% and 1.6%, respectively. 3. Area centralis was located at the distance of 1.6mm (1-week-old) and 2.0mm(7-week-old) nasal side from the anterior tip of the pecten. The number of ganglion cells in the area centralis was 11,000 cells/㎟ (1-week-old) and 12,000 cells/㎟ (7-week-old). 4. There was a gradual decrease in density of retinal ganglion cells extending from area centralis to the periphery of retina. 5. The density of the large (above 20㎛) ganglion cells was higher in the peripheral regions than in the center of retina. Higher densities were noted in the superior-temporal region in 7-week-old duck, and in the nasal region in 1-week-old duck than in the other regions. 6. The direction of the beak tip is approximately 30° in 1-week-old and 26° in 7-week-old duck from the angle of the pecten.

Cisplatin과 Tobramycin이 白鼠 Corti器에 미치는 영향에 關한 電子 顯微鏡的 硏究 : Ultrastructural Study

김동환,신태균,김무강,김홍선,김원식 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.1

In order to study the morphological changes of Corti’s organ in the rat treated with cisplatin and tobramycin, thirty-three rats, weighing between 200∼250g, were used, and divided into 4 groups, i.e., 3 rats were used for control, and 30 rats were used for 3 experimental groups. Each experimental group was divided into cisplatin-treated, tobramycin-treated, and both cisplatin and tobramycin treated group with 10 rats. Rats were injected with a single dose of 7.7㎎/㎏ of cisplatin intraperitoneally and 50㎎/㎏/day of tobramycin for 2 days intramuscularly, respectively or both. Treated rats were sacrificed on the 3rd and 5th day after the first injection. Results were as follows; 1. Cisplatin-treated group: Outer hair cells of Corti’s organ were degenerated with the formation of vacuoles and appearance of lysosomes, however, inner hair cells showed mild vacuolization. 2. Tobramycin-treated group: NO pathological changes were found in Corti’s organ. 3. Combined cisplatin and tobramycin treated group: Outer and inner hair cells showed vacuolization, appearance of lysosomes, myelin figures and multivesicular body. There were also multivesicular body in the inner phalangeal cell and myelin figure in the Reissner’s membrance. 4. Degeneration of Corti’s organ in the combined treated group was more sever than those of the single cisplatin-treated or tobramycin-treated group. 5. NO pathological differences of Corti’s organ in duration of postinjection were manifested in any groups. It can be concluded that cisplatin induces toxicity to inner ear and cisplatin enhance toxicity more in continued treated with tobramycin.

오리 鼻腔 및 鼻粘膜上皮의 計測에 관한 硏究

金世仁,金武剛,申台均,金弘善,柳時潤,李永浩 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.1

The area of the various epithelial types linging the nasal navity of each six number in 1-week-old and 10-week-old adult ducks has been made for the light microscopic research. Transverse sections of 10 microns thick in each specimen were made continuously from the anterior end of nostril to the posterior end of pharyngeal inlet and specimen samples were selected at 0.13mm(1-week-old) and 0.17mm(10-week-old) intervals from the anterior end of the nasal cavity to the posterior end of pharyngeal inlet. The results obtained from research were summarized as follows. 1. The nasal cavity is lined with stratified squamous, respiratory and olfactory epithelium. The thickness of stratified squamous, respiratory and olfactory epithelium is 10-100㎛, 8-75㎛ and 50㎛ respectively in 1-week-old ducks, and 25-175㎛, 20-125㎛ and 66㎛ respectively in 10-week-old ducks. 2. The unilateral volumes of the nasal cavity in 1-week-old and 10-week-old ducks were 21.73±2.64㎟, 1,692.44±171.57㎟ respectively. 3. The total unilateral surface areas of the nasal cavity in 1-week-old and 10-week-old ducks were 70.44±4.92㎟, 1,772.45±94.10㎟ respectively. 4. Each area of the stratified squamous epithelium, respiratory epithelium and olfactory epithelium in unilateral nasal cavity is 14.82±1.85㎟ (21.1%), 46.86±3.20㎟ (66.5%) and 8.76±0.40㎟ (12.4%) in the 1-week-old ducks, and 348.23±40.02㎟ (19.7%), 1,282.31±47.03㎟ (72.3%) and 141.90±12.43㎟ (8.0%) in the 10-week-old ducks. Ducks are assumed as a microsmatic animals by the composition of the lining epithelium of the nasal cavity in ducks.

오리 嗅粘膜의 組織學的 및 組織化學的 硏究

朴在贊,金武剛,金弘善,申台均 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.1

This paper dealt with the histological feature of the Pekin duck olfactory mucosa and the specificity of the types of mucopolysaccharides present in the secretory cells of the duck olfactory mucosa. The nasal parts of ducks (1-week-old and 10-week-old) were fixed with 10% neutral formalin, decalcified, embedded in paraffin. Serial sections were cut 5 um thick and stained with alcian blue(pH 2.5) to demonstrate acidic mucopolysaccharides and the periodic acid-Schiff reaction to identify neutral mucopolysaccharides. The results were summarized as follows. 1. The duck olfactory mucosa consisted of the olfactory epithelia which compose of the receptor neurons, supporting cells, basal cells and goblet cells, and the lamina propria which contained Bowman's glands, olfactory nerve bunbles and connective tissue cells. 2. The thickness of the olfactory mucosa was 121.5±25.67um in the 1-week-old ducks, and 202±45.82km in the 10-week-old ducks. Increased thickness of olfactory mucosa was due to the increased thickness of lamina propria. 3. Histochemical techniques of the olfactory mucosa in 10-week-old ducks demonstrated that Goblet cells, sppporting cells and Bowman's gland contained both neutral and acidic mucopolysacc harides, the neutral mucopolysaccharides staining became enhanced first in the Goblet cells and Bowman's gland, and then in supporting cells. In 1-week-old ducks, both acidic and neutral mucopolysaccharides of Goblet cells reacted strongly, but those of supporting cells and Bowman's gland reacted presumably.

오리 선위 점액분비세포의 점액질에 대한 조직화학적 연구

류시윤,김홍선,김무강,신태균,이영호 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

Histochemical sequential staining techniques, PAS, alcian blue pH 2.5 and pH 0.4, were applied to sections from the proventriculus of ducks to observe the histochemical properties of epithelial mucosubstances in the proventriculus of mallard and white Pekin ducks. The whole proventriculus obtained were divided systemically cross strips and processed routinely for histologic and histochemical study. Base on the histologic characteristics, we designated as upper portion which is the distal region of the esophageal-proventriculus junction, as middle portion, and as lower portion which is the proximal region of the proventricular-isthmus junction. The results obtained are as follows 1. Generally in the surface and foveolar epithelia showed the presence of the mixture of neutral and acid mucosubstances. 2. In the tubular gland showed the acidic nonsulfated and sulfated mucosubstance in addition to neutral mucosubstances with occasional neutral and acidic nonsulfated mucosubstances. 3. In the compound gland neutral mucosubstance with scant amounts of acidic mucosubstances or with lack of it were demonstrated. 4. There were recognized differences between speies in the staining intensity of all three types of mucosubstances. 5. There were recognized topographic difference in the acidity of epithelial mucosubstances according to portion.

항혈청 투여에 따른 돼지 전염성 설사병 예방효과 : Ⅰ.혈청학적 결과, RT-PCR 검사, 형광항체검사 Ⅱ.Serological Results, Rt-PCR for Fecal and Small Intestin, FA Test

지영철,한정희,권혁무,한태욱,정현규,박봉균 한국수의병리학회 2002 한국수의병리학회지 Vol.6 No.1

The purpose of this study was to investigate to potective effects against porcine epidemic diarrhea virus (REDV) infection in piglets by administration of the PEDV antiserum orally at 2 hrs, 24 hrs and 36 hrs after birth. six piglets adiminstered the antiserum were experimentally infected with PEDV at five-day-old. Control group were four piglets infected with PEDV only. Serum antibody titers aginst PEDV were examined by serum neutralization (SN) test, dectection for PEDV or PEDV antigen from feces and small intestines was tested by reverse transcrption-polymerase chain reaction (RT-PCR) and indirect immunoflurescence (IFA). The results obtained were as follows; 1. The piglets administered the PEDV antiserum showed higher antibody titers than those of control group and sustained during the experimental period. 2. The detection rate of PEDV in feces and small intestines by RT-PCR were 26.2% and 16.7% in PEDV antiserum treated group and 48.1% and 75.0% in control group, respectively. 3. The detection rate of PEDV antigen in the small intestine by IFA were 0% in PEDV antiserum treated group and 50.0% in control group, respectively. It was concluded that oral administration of antiserum against PEDV to piglets was effective in preventing PEDV infection.