IL-15에 의한 류마티스관절염 환자 활막 섬유모세포에서의 SDF-1 유도

박영은 ( Young Eun Park ),김성일 ( Sung Il Kim ),박성후 ( Seong Hu Park ),백승훈 ( Seung Hoon Baek ),오혜좌 ( Hye Jwa Oh ),허양미 ( Yang Mi Heo ),조미라 ( Mi La Cho ) 대한류마티스학회 2010 대한류마티스학회지 Vol.17 No.3

Objective: Interleukin-15 (IL-15) recruits and activates synovial T cells, and IL-15 plays an important role in amplifying and perpetuating inflammation in the pathogenesis of rheumatoid arthritis (RA). Stromal cell-derived factor-1 (SDF-1) is a potent chemoattractant for memory T cells in the inflamed RA synovium. This study investigated the effect of IL-15 on SDF-1 production in RA fibroblast-like synoviocytes (FLS). Methods: The expressions of IL-15 and SDF-1 were determined from the synovium of patients with RA and osteoarthritis (OA) by performing immunohistochemistry. The expressions of SDF-1 was measured from the RA FLS that were cultured with IL-15 and IL-17 by real-time RT-PCR and ELISA. The SDF-1 expression was also measured, via ELISA, from the RA FLS stimulated by IL-15 together with the inhibitors of such intracellular signal molecules as phosphatidylinositol 3-kinase (PI 3-kinase, LY294002), STAT3 (AG490), MAP Kinase (PD98059), NF-κB (parthenolide) and activator protein 1 (AP-1, curcumin). Results: IL-15 and SDF-1 were mainly expressed in the RA synovium compared to that of the OA synovium. IL-15 increased the SDF-1 expressions and it, and had an additive effect with IL-17 on the SDF-1 expressions in the cultured RA FLS. The IL-15 induced increase of the SDF-1 expression in the cultured RA FLS was blocked by the inhibitors of PI 3-kinase, NF-κB and AP-1. Conclusion: The SDF-1 expression was increased in the RA synovium and it was up-regulated by IL-15 in the RA FLS through the PI 3-kinase, NF-κB, and AP-1 pathways. These results imply that the IL-15 induced increase of the SDF-1 expressions may be involved in the immunopathogenesis of RA.

류마티스 관절염 동물 모델에서 활막의 RANKL/OPG mRNA 발현 비율 및 IL-17의 효과

이준희 ( Jun Hee Lee ),김근태 ( Geun Tae Kim ),류선 ( Sun Ryu ),김주인 ( Ju In Kim ),백승훈 ( Seung Hoon Baek ),김성일 ( Sung Il Kim ) 대한류마티스학회 2006 대한류마티스학회지 Vol.13 No.2

Objective: To investigate the synovial mRNA expression of receptor activator of NFκB (RANK), RANK ligand (RANKL), osteoprotegerin (OPG) and RANKL/OPG mRNA expression ratio, and to evaluate the effects of IL-17 in experimental rheumatoid arthritis (RA) model. Methods: After induction of collagen-induced arthritis (CIA) by type II collagen in DBA1 mice, mice were anesthetized at day 28 and a small aperture in the skin of the knee was performed. Mice, in which arthritis of knee was present, were selected and divided into 3 groups, and phosphate-buffered saline (PBS group), IL-17 (IL-17 group) or anti-IL-17 monoclonal antibody (anti-IL-17 group) was injected to both knee joint at day 28 and 32. At day 35, mice were sacrificed and synovium of knee joints were isolated. Synovial mRNA expression of RANKL, RANK and OPG was assessed by real-time RT-PCR and immunohistochemical stain. Results: Synovial RANKL and RANK mRNA expressions were significantly different among IL-17, PBS, anti-IL-17 and normal group (IL-17>PBS>anti-IL-17>normal group), and synovial OPG mRNA expressions in PBS, IL-17 and anti-IL-17 group were significantly high than those in normal group, however, there was no significant difference among IL-17, PBS and anti-IL-17 group. RANKL/OPG mRNA ratio was significantly different among these groups (IL-17>PBS>anti-IL-17>normal group). In immunohistochemical stain, RANKL, RANK and OPG-positive cells were expressed at synovium. Conclusion: Synovial RANKL/OPG mRNA ratio was enhanced in CIA, and IL-17 induced higher RANKL/OPG ratio in the synovium of CIA, which was blocked by anti-IL-17 antibody. These results suggest that RANKL/OPG mRNA ratio play an important roles on bone destruction, and IL-17 may be actively involved in bone destruction by enhancing RANKL/OPG ratio in CIA model.

Interleukin-17이 배양된 류마티스관절염 활막세포에서 vascular endothelial growth factor 생성에 미치는 영향

곽임수 ( Ihm Soo Kwak ),남태수 ( Tae Soo Nam ),나하연 ( Ha Yeon Rha ),서정탁 ( Jeung Tak Suh ),김유선 ( Yoo Sun Kim ),김성일 ( Sung Il Kim ) 대한류마티스학회 2001 대한류마티스학회지 Vol.8 No.3

Objective: To investigate the the effects of interleukin-17 (IL-17) on the production of vascular endothelial growth factor (VEGF) from cultured rheumatoid arthritis synoviocytes. Methods: Fibroblast-like synovial cells(FLS) were prepared from the synovial tissues of rheumatoid arthritis patients and cultured in the presence of IL-17, IL-17 with or without transforming growth factor-β(TGF-β), tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β). VEGF levels were determined in the culture supernatants by sandwitch ELISA. Results: Stimulation of FLS by serial concentration of IL-17, TGF-β, TNF-α, IL-1β increased the production of VEGF by 2.1-2.7, 2.2-3.0, 2.0-2.9, 2.3-3.1 fold over the constitutive levels of unstimulated FLS. Stimulation of FLS by IL-17 with TGF-β or TNF-α or IL-1β also increased the production of VEGF according to culture periods by 1.6-1.8, 1.1-1.9, 1.5-1.7 fold over the levels stimulated with TGF-β or TNF-α or IL-1β, respectively. This results indicated that IL-17 increased the effect of TGF-β, TNF-α, IL-1β on FLS, leading synergistic enhancement of VEGF production. Conclusion: IL-17 may be involved in the neovascularization in rheumatoid synovitis by enhancing the production of VEGF.

자유기에 의한 지질과산화 반응에 대한 황금 약침액의 항산화 효능

김성일,문진영,김갑성,김두희,남경수,임종국,Kim Sung-Il,Moon Jin-Young,Kim Kap-Sung,Kim Doo-Hie,Nam Kyung-Soo,Lim Jong-Kook 대한예방한의학회 1997 대한예방한의학회지 Vol.1 No.1

Scutellariae radix, has been used as a natural drug for fever, inflammation, cataract, and liver disease in traditional medicine. This study was performed in order to investigate the antioxidative effects of Scutellariae radix aqua-acupuncture solution (SRAS) on lipid peroxidation by free radicals. Lipid peroxidation levels were determined by TBA method during the autoxidation of linoleic acid. In this linoleic acid autoxidation system, SRAS markedly exhibited antioxidant activity, which inhibited 89% of linoleic acid peroxidation. SRAS showed scavenging effects on ${\alpha},{\alpha}-diphenyl-{\beta}-picrylhydrazyl$(DPPH) radical, inhibited superoxide generation in xanthine-xathine oxidase system, and also inhibited lipid peroxidation of rat liver tissue by hydroxyl radical derived from $H_2O_2-FE^{+2}$ system. These effects were similar to those of $dl-{\alpha}-tocopherol$, BHA and BHT. In addition, SRAS protected the cell death induced by ter-butyl hydroperoxide (t-BHP) and significantly increased cell viability in the normal rat liver cell (Ac2F). On the basis of these results, it is suggested that SRAS might play a protective role in lipid peroxidation by free radicals.

동안신경마비를 동반한 안와 국균증 1예

김성일,최희영,이지웅,Sung Il Kim,Hee Young Choi,Ji Woong Lee 대한안과학회 2014 대한안과학회지 Vol.55 No.1

Purpose: To report a case of orbital aspergillosis with third nerve palsy. Case summary: A 75-year-old male presented with abrupt onset of visual impairment, extraocular movement limitation, and ptosis. The patient previously experienced rhinolalia and headache and was diagnosed with sinusitis 2 months prior, and was treated with oral antibiotics for 1 week. Orbital magnetic resonance imaging revealed a mass with bone erosion including the nasal cavity, ethmoid bone, and left orbit suggestive of fugal sinusitis. Aspergillus was detected histopathologically in the mass which was removed by endoscopic surgery. Amphotericin B was administered intravenously for 7 days along with voriconazole. There was no recurrence during the follow-up period. Extraocular movement limitations and ptosis were recovered postoperatively. Conclusions: The present study results indicate that visual impairment and third nerve palsy can develop in a patient with orbital aspergillosis. J Korean Ophthalmol Soc 2014;55(1):149-154

이더넷 광 네트워크 구현을 위한 1.25 Gbps 광전 트라이플렉스 트랜시버 모듈의 전기적 혼신의 분석

김성일,이해영,Kim Sung-Il,Lee Hai-Young 대한전자공학회 2005 電子工學會論文誌-SD (Semiconductor and devices) Vol.42 No.3

본 논문에서는 이더넷 광 네트워크 구현을 위한 핵심 부품인 1.25 Gbps 광전 트라이플렉스 트랜시버 모듈(Opto-electric triplex transceiver module)의 동작성능 안정화를 위하여 모듈내에서 발생되는 전기적 혼신을 해석 및 측정하였으며, 혼신 감소를 위한 가상접지선(Dummy ground line)이 포함된 신호선 구조를 제안하였다. 광전 트라이플렉스 트랜시버 모듈은 전기신호를 광신호로 바꾸어 전송하는 송신부(Laser diode), 디지털 변조되어 입력된 광신호를 전기신호로 변환하는 디지털 수신부 (Digital photodetector)와 고해상도의 CATV (Community antenna or access television) 신호를 수신하는 아날로그 수신부 (Analog photodetector)가 실리콘 기판(Silicon substrate) 상에 하이브리드 집적되어 구성된다. 디지털 수신부와 아날로그 수신부의 수신감도는 각각 BER(Bit error rate) : $10^-{12}$에서 -24 dBm과 44 dB의 신호대잡음비(Signal-to-noise ratio, SNR)에서 -7.7 dBm을 만족해야하므로 모듈 내의 전기적 혼신은 DC에서 3 GHz까지 - 86 dB이하로 유지되어야한다. 전기적 혼신의 해석 및 측정 결과, 실리콘 기판상의 광원과 디지털 광검출기, 디지털 광검출기와 아날로그 광검출기 사이의 거리를 4 mm 이상 확보하며, 가상접지선을 디지털 광검출기와 아날로그 광검출기의 신호선과 $100\;{\mu}m$ 간격으로 설치하였을 경우, -86 dB 이하의 전기적 혼신 레벨을 만족할 수 있음을 확인하였다. 본 논문에서 제안한 가상접지선을 사용하는 방법은 실리콘 기판상에 신호선을 형성할 때 동시에 형성할 수 있으므로 별도의 추가비용 없이 구현할 수 있으며, 단순히 광원 및 광 검출기의 사이간격을 충분히 확보하는 방법에 비하여 실리콘 기판의 크기를 감소시켜 최종 모듈의 크기를 약 $50\%$ 감소시킬 수 있다는 장점이 있다. In this paper, we analyzed and measured the electrical crosstalk characteristics of a triplex transceiver module for ethernet Passive optical networks(EPONS). And we improved the electrical crosstalk levels using Dummy ground lines with signal lines. The triplex transceiver module consists of a laser diode as a transmitter, a digital photodetector as a digital data receiver, and a analog photodetector as a community antenna television signal receiver. And there are integrated on silicon substrate. The digital receiver and analog receiver sensitivity have to meet -24 dBm at $BER=10^{-l2}$ and -7.7 dBm at 44 dB SNR. And the electrical crosstalk levels have to maintain less than -86 dB from DC to 3 GHz. From analysis and measurement results, the proposed silicon substrate structure that contains the Dummy ground line with $100\;{\mu}m$ space from signal lines and separates 4 mm among devices respectively, is satisfied the electrical crosstalk level compared to simple structure. This proposed structure can be easily implemented with design convenience and greatly reduced the silicon substrate size about $50\%$.

Quatrz 웨이퍼의 직접접합과 극초단 레이저 가공을 이용한 체내 이식형 혈압센서 개발

김성일,김응보,소상균,최지연,정연호,Kim, Sung-Il,Kim, Eung-Bo,So, Sang-kyun,Choi, Jiyeon,Joung, Yeun-Ho 대한의용생체공학회 2016 의공학회지 Vol.37 No.5

In this paper we present an implantable pressure sensor to measure real-time blood pressure by monitoring mechanical movement of artery. Sensor is composed of inductors (L) and capacitors (C) which are formed by microfabrication and direct bonding on two biocompatible substrates (quartz). When electrical potential is applied to the sensor, the inductors and capacitors generates a LC resonance circuit and produce characteristic resonant frequencies. Real-time variation of the resonant frequency is monitored by an external measurement system using inductive coupling. Structural and electrical simulation was performed by Computer Aided Engineering (CAE) programs, ANSYS and HFSS, to optimize geometry of sensor. Ultrafast laser (femto-second) cutting and MEMS process were executed as sensor fabrication methods with consideration of brittleness of the substrate and small radial artery size. After whole fabrication processes, we got sensors of $3mm{\times}15mm{\times}0.5mm$. Resonant frequency of the sensor was around 90 MHz at atmosphere (760 mmHg), and the sensor has good linearity without any hysteresis. Longterm (5 years) stability of the sensor was verified by thermal acceleration testing with Arrhenius model. Moreover, in-vitro cytotoxicity test was done to show biocompatiblity of the sensor and validation of real-time blood pressure measurement was verified with animal test by implant of the sensor. By integration with development of external interrogation system, the proposed sensor system will be a promising method to measure real-time blood pressure.

사료 내 생 미강과 볶은 대두의 첨가가 거세한우 등심의 이화학적 특성과 관능적 특성에 미치는 영향

김성일,정근기,김덕영,김진열,최창본,Kim, Sung-Il,Jung, Keun-Ki,Kim, Duck-Young,Kim, Jin-Yeoul,Choi, Chang-Bon 한국축산식품학회 2011 한국축산식품학회지 Vol.31 No.3

본 연구는 거세한우 사료 내 미강과 볶은 대두를 첨가 함으로써 거세한우 도체등심의 이화학적 특성과 관능적 특성에 미치는 영향을 조사하고자 실시되었다. 평균 20.92개월령(평균체중 599.89 kg)의 거세한우 36두를 대조구, 시험 1구(미강 첨가구) 및 시험 2구(볶은 대두 첨가구)로 나누고 각각의 처리구는 체중 및 월령을 고려하여 배치하였다(12두/처리구). 미강과 볶은 대두의 첨가 수준은 시험 사료 내 조지방 함량을 5%로 설정하고, 조지방 부족분을 각각의 첨가원료(미강과 볶은 대두)로부터 보충 되도록 설계하였으며, 시험동물이 평균 31.2개월령에 도달할 때까지 314일간 사양시험을 실시하였다. 도체등심의 일반 조성분은 처리구별 뚜렷한 차이는 없었고 가열 감량과 드립 감량도 처리구별 유의적인 차이는 없었다. 체지방 부위에 따른 처리구별 융점은 뚜렷한 차이가 없었으나, 체지방 부위별 융점은 등심지방($25.83-26.17^{\circ}C$), 피하지방($17.26-18.53^{\circ}C$) 및 신장지방($32.40-33.15^{\circ}C$)이 서로 다른 범위를 나타내었다. 도체등심의 단맛과 관련이 있는 유리 아미노산은 시험 1구가 39.48 mg/100 g으로서 대조구의 32.24mg/100 g에 비하여 유의적(p<0.05)으로 높게 나타났으며, 쓴맛과 관련이 있는 유리 아미노산은 시험 2구가 25.93mg/100 g으로서 다른 처리구에 비하여 유의하게(p<0.05) 낮게 나타났다. 도체등심의 지방산 조성 중 $C_{14:0}$, $C_{16:0}$, $C_{16:1}$, $C_{18:0}$ 및 $C_{18:1}$의 함량은 처리구별 유의적인 차이가 없었으며, 다가불포화지방산인 $C_{20:3}$은 시험 2구가 다른 처리구에 비하여 유의적(p<0.05)으로 높게 나타났다. 도체등심의 관능평가 결과는 다즙성의 경우 시험 1구가 가장 높게 나타났으며(p<0.05), 종합기호 도의 경우는 시험 1구와 시험 2구가 대조구에 비하여 유의적으로(p<0.05) 높게 나타났다. 도체등심의 관능 평가 항목들과 주요지방산 조성간의 상관관계는 $C_{18:1}$은 연도와, $C_{18:0}$은 풍미와 정(+)의 상관관계(각각 r=0.650 및 0.698; p<0.05)를 나타내었다. 이상의 연구 결과를 종합해 보면 사료 내 미강과 볶은 대두의 첨가가 거세한우 등심의 이화학적 특성에는 처리구별 뚜렷한 차이를 보이지 않았지만 관능적 특성을 개선시키는 데에는 효과적이었던 것으로 나타났다. We investigated melting points and sensory characteristics by adding rice bran and roasted soybean to the finishing diet of Hanwoo steers. Thirty-six Hanwoo steers (average age, 20.92 mon-old; average weight, 599.89 kg) were assigned to either Control, Treatment 1 (rice bran) or Treatment 2 (roasted soybean) groups (12 steers/group), considering body weight and age. Supplementation levels of rice bran and roasted soybean were determined to meet total 5% crude fat in the concentrates, and the experimental animals were fed for 314 d until they reached 31.2 mon old. No significant differences were found in the moisture and crude fat content among groups. Melting points of lipid extracted from M. longissimus dorsi, subcutaneous fat, and perirenal fats were 25.83-26.17, 17.26-18.53 and $32.40-33.15^{\circ}C$, respectively, resulting in remarkable differences depending on fat depots. Contents of free amino acids in M. longissimus dorsi related to sweet taste were 39.48 mg/100 g for Treatment 1, which was significantly (p<0.05) higher than those for Control (32.24 mg/100 g), whereas those related to bitter taste were 25.93 mg/100 g for Treatment 2, which was the lowest (p<0.05) among the groups. Fatty acid composition, such as $C_{14:0}$, $C_{16:0}$, $C_{16:1}$, $C_{18:0}$, and $C_{18:1}$, in M. longissimus dorsi was not different among the groups. Results of a panel test on M. longissimus dorsi showed that Treatment 1 scored the highest (p<0.05) in juiciness, and both Treatments 1 and 2 scored higher (p< 0.05) in overall palatability than the Control group. $C_{18:1}$ showed a positive (+) relationship with tenderness (r = 0.650; p<0.05), and $C_{18:0}$ with flavor (0.698; p<0.05). The results suggest that rice bran and roasted soybean are beneficial for improving M. longissimus dorsi sensory characteristics, whereas no significant differences were found in the physico-chemical characteristics among the groups.

전방깊이에 따른 백내장수술 전후의 각막내피세포수의 변화

김성일,나경선,권형구,이현수,김만수,Sung Il Kim,Kyung Sun Na,Hyung Gu Kwon,Hyun Soo Lee,Man Soo Kim 대한안과학회 2010 대한안과학회지 Vol.51 No.12

Purpose: To evaluate the effects of preoperative and postoperative parameters, particularly anterior chamber depth, on corneal endothelial cell loss during cataract surgery. Methods: Eighty-two eyes of 82 patients who underwent cataract surgery by the same surgeon using the same technique were selected for the present study. Various preoperative and postoperative parameters including age, anterior chamber depth, duration and power of phacoemulsification, preoperative endothelial cell density, coefficient of variation, hexagonality, and grade of nucleosclerosis were evaluated. Endothelial cell densities were recorded preoperatively and at two months postoperatively using a noncontact specular microscope. Results: After two months, the mean central endothelial cell loss in all eyes was 8.77%. Among the pre- and post-operative parameters, patient’ age, anterior chamber depth, duration, power, and energy of phacoemulsification and grade of nucleosclerosis showed significant correlation with endothelial cell loss. Stepwise multiple linear regression analysis identified age, nucleosclerosis, anterior chamber depth, and phacoemulsification time as independent predictors for endothelial cell loss. Conclusions: The risk of endothelial cell loss increased with increases in patient’ age, nucleosclerosis, and phacoemulsification time. However, eyes with a deep anterior chamber showed a significantly lower risk of endothelial cell loss. J Korean Ophthalmol Soc 2010;51(12):1568-1572

부동산 개발사업 평가기준 및 지표 개발

김성일,배유진,장철기,Kim, Sung-Il,Bae, Yu-Jin,Chang, Chul-Ki 한국건설관리학회 2015 건설관리 : 한국건설관리학회 학회지 Vol.16 No.2

2000년대 PF(Project Financing)의 적극 활용을 통해 부동산개발 및 PF 시장이 급성장하였지만, 이후 세계금융위기의 여파로 부동산 경기 침체로 인해 상당수의 개발사업이 정상적으로 추진되지 못하게 되었다. 이러한 현상은 철저한 사업성 분석 없이 무리하게 사업을 추진한 것이 근본적인 원인으로 판단된다. 이에 본 연구에서는 부동산개발사업의 무분별한 추진을 억제하고 객관적인 평가가 가능하도록 기존 부동산개발사업 평가체계의 문제점을 짚어보고 이를 보완할 수 있는 부동산개발사업의 평가항목 및 평가기준 등을 평가수요 및 사업유형 등 사업의 성격에 맞게 적용할 수 있는 전반적인 평가체계를 제안하였다. 본 연구에서 제안한 부동산 평가체계 및 운영방안을 통해 부동산 평가체계를 제도화하여 표준화함으로써, 보다 일관성 있는 평가가 가능할 것으로 기대되며, 아울러 평가자료의 통일적 축적이 가능하게 될 것으로 기대된다. Real estate market has rapidly grown by active use of project financing in the early 2000s. However, many projects have been in trouble due to global economic recession after that period. It is mainly because those projects were pushed forward a business without comprehensive business feasibility analysis. This paper investigated current evaluation system for real estate development projects and then introduced new evaluation system including evaluation factor and criteria for real estate development projects to prevent implementation of the project without business value and to be able to evaluate potential projects in objective manner. This new evaluation system for real estate development projects can be applied to any type of real estate development projects by modifying evaluation items and weighting factor in accordance with the type and characteristics of project. Through the new evaluation system, the evaluation process for real estate development projects will be able to be standardized, be consistent, and the results of evaluation can be accumulated in consistent way.