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Whole-exome sequencing identifies recurrent <i>AKT1</i> mutations in sclerosing hemangioma of lung
Jung, Seung-Hyun,Kim, Min Sung,Lee, Sung-Hak,Park, Hyun-Chun,Choi, Hyun Joo,Maeng, Leeso,Min, Ki Ouk,Kim, Jeana,Park, Tae In,Shin, Ok Ran,Kim, Tae-Jung,Xu, Haidong,Lee, Kyo Young,Kim, Tae-Min,Song, Sa National Academy of Sciences 2016 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.113 No.38
<P>Pulmonary sclerosing hemangioma (PSH) is a benign tumor with two cell populations (epithelial and stromal cells), for which genomic profiles remain unknown. We conducted exome sequencing of 44 PSHs and identified recurrent somatic mutations of AKT1 (43.2%) and beta-catenin (4.5%). We used a second subset of 24 PSHs to confirm the high frequency of AKT1 mutations (overall 31/68, 45.6%; p.E17K, 33.8%) and recurrent beta-catenin mutations (overall 3 of 68, 4.4%). Of the PSHs without AKT1 mutations, two exhibited AKT1 copy gain. AKT1 mutations existed in both epithelial and stromal cells. In two separate PSHs from one patient, we observed two different AKT1 mutations, indicating they were not disseminated but independent arising tumors. Because the AKT1 mutations were not found to co-occur with beta-catenin mutations (or any other known driver alterations) in any of the PSHs studied, we speculate that this may be the single-most common driver alteration to develop PSHs. Our study revealed genomic differences between PSHs and lung adenocarcinomas, including a high rate of AKT1 mutation in PSHs. These genomic features of PSH identified in the present study provide clues to understanding the biology of PSH and for differential genomic diagnosis of lung tumors.</P>
오픈 소스 기반 데이터 분산 중복제거 파일 시스템의 성능 분석
정성욱(Sung-ouk Jung),최훈(Hoon Choi) 한국정보과학회 2014 정보과학회 컴퓨팅의 실제 논문지 Vol.20 No.12
데이터 중복제거 파일시스템인 LessFS와 SDFS의 성능을 비교하면, LessFS는 CPU 점유율과 수행 시간에서 성능이 우수하고, SDFS는 중복제거 이후 저장소 사용량이 다른 파일시스템보다 1/8 정도의 이점을 가지고 있다. 본 논문은 SDFS의 장점인 중복제거 이후 저장소 사용량 감소와 LessFS의 장점인 낮은 CPU 점유율과 수행 시간 감소의 장점을 지니는 새로운 방식을 제안한다. SDFS의 Dedup File Engines (DFE) n개를 이용하되, Dedup Storage Engines (이하 DSE) 1개를 두어 중복제거 데이터의 정합성과 일관성을 유지하는 방식이다. 제안하는 방식을 2개의 DFE와 1개의 DSE를 가진 시험환경에 구현하고 성능 비교를 수행한다. Comparison of two representative deduplication file systems, LessFS and SDFS, shows that Lessfs is better in execution time and CPU utilization while SDFS is better in storage usage (around 1/8 less than general file systems). In this paper, a new system is proposed where the advantages of SDFS and Lessfs are combined. The new system uses multiple DFEs and one DSE to maintain the integrity and consistency of the data. An evaluation study to compare between Single DFE and Dual DFE indicates that the Dual DFE was better than the Single DFE. The Dual DFE reduced the CPU usage and provided fast deduplication time. This reveals that proposed system can be used to solve the problem of an increase in large data storage and power consumption.
Macrolide계 항생물질 동시분석법 확립 및 모니터링
박상욱(Sang-Ouk Park),이상호(Sang-Ho Lee),안종훈(Jong-Hoon Ahn),정영지(Young-Ji Jung),김성철(Seong-Cheol Kim),김지연(Ji-Yeon Kim),금은희(Eun-Hee Keum),성주현(Ju-Hyun Sung),김상엽(Sang-Yub Kim),장영미(Young-Mi Jang),강찬순(Chan-Soon K 한국식품과학회 2010 한국식품과학회지 Vol.42 No.3
축산물 중 macrolide계 항생물질 3종을 신속분석하기 위하여 효과적인 전처리법을 설정하고 고속액체크로마토그라피(HPLC)를 이용한 동시분석법을 제시하였다. 대상물질은 tilmicosin, tylosin, spiramycin이며, 확립된 분석법을 이용하여 모니터링을 실시하기 위하여 전국 6개 도시인 서울, 부산, 대전, 인천, 대구, 광주에서 수입원산지가 표시된 쇠고기와 돼지고기 및 그 가공품을 수거하여 분석하였다. 전처리법에 있어서 solid phase extraction(SPE)법에 비하여 액상추출법이 더 높은 회수율을 나타내었으며 전처리 단계도 간단하여 대상 항생물질을 분석하기에 적절하였다. 3종의 항생물질 분리를 위한 컬럼은 C18(250 ㎜×4.6 ㎜, 5 ㎛)을 사용하였으며, HPLC 이동상은 0.025M phosphate buffer(pH 2.5) 및 acetonitrile을 이용한 gradient 조건을 설정하였다. UV 검출파장은 spiramycin 경우 232 ㎚이고, tilmicosin과 tylosin은 288 ㎚을 이용하였다. 평균회수율은 83.0-90.2%이였으며, 검출한계는 각각 7(spiramycin), 12(tilmiconsin), 12(tylosin) ng/g으로 나타났다. 수입 축산물의 항생물질에 대한 안전성을 검토하기 위하여 국내 유통중인 수입축산물 및 그 가공품을 대상으로 하여 모니터링을 실시한 결과, 시료는 전국 6개 대도시에서 126건 구입하였으며 모든 시료에서 macrolide계 항생물질이 검출되지 않았다. In this study, a simple and rapid pre-treatment method based on liquid extraction was applied for the simultaneous determination of three macrolides (spiramycin, tylosin, and tilmicosin) residues. In these studies, the stock farm products was used as a matrix sample. When the liquid extraction method was compared with the solid phase extraction (SPE) method, the former showed higher recovery percentages and simpler steps than the latter. The macrolids were separated using a reverse-phase C18 (250 ㎜×4.6 ㎜, 5 ㎛) column and a gradient elution with mobile phases consisting of phosphate buffer (pH 2.5) and acetonitrile. Tylosin and tilmicosin were detected at 288 ㎚ and spiramycin was detected at 232 ㎚. The average recovery percentage ranged between 83.0-90.2% for samples spiked with the three macrolids at 50 and 100 ng/g The validation results showed that the limit of detection (7 (spiramycin), 12 (tilmiconsin), 12 (tylosin) ng/g)) was under the regulatory tolerances and the linearity from calibration curves was satisfactory for determining the multi-residue of three macrolids in farm products. Monitoring samples were collected at the main cities in Korea as Seoul, Busan, Deajeon, Incheon, Deagu, and Gwangju. Microlide antibiotics were not detected in most samples.