Effect of Beverage Containing Fermented Akebia quinata Extracts on Alcoholic Hangover

Suhan Jung,Sang Hoon Lee,Young Sun Song,Seo Yeon Lee,So Young Kim,Kwang Suk Ko 한국식품영양과학회 2016 Preventive Nutrition and Food Science Vol.21 No.1

The present study was conducted to investigate the effects of beverages containing fermented Akebia quinata extracts on alcoholic hangover. For this study, 25 healthy young men were recruited. All participants consumed 100 mL of water (placebo), commercial hangover beverage A or B, fermented A. quinata leaf (AQL) or fruit (AQF) extract before alcohol consumption. After 1 h, all participants consumed a bottle of Soju, Korean distilled liquor (360 mL), containing 20% alcohol. Blood was collected at 0 h, 1 h, 3 h, and 5 h after alcohol consumption. The plasma alanine transaminase (ALT) activity was highest in the placebo group. Compared with the control group, the AQL and AQF groups showed decreased ALT activity at 5 h after alcohol consumption. Plasma ethanol concentration was increased after alcohol intake and peaked at 3 h after alcohol consumption. Compared with the control group, the A group showed a higher plasma ethanol concentration at 1 h (P<0.05). At 3 h after alcohol consumption, the AQF group showed the lowest mean plasma ethanol concentration compared to the other groups; however, there were no statistical differences. After 5 h of alcohol consumption, the AQL and AQF groups showed lower plasma ethanol concentrations compared with the B group. The sensory evaluation score for the fermented A. quinata fruit extract was lower than for the commercial hangover beverages. In conclusion, the present intervention study results suggest that fermented A. quinata extracts alleviate alcoholic hangover and reduce plasma ethanol concentrations.

Effect of Beverage Containing Fermented Akebia quinata Extracts on Alcoholic Hangover

Jung, Suhan,Lee, Sang Hoon,Song, Young Sun,Lee, Seo Yeon,Kim, So Young,Ko, Kwang Suk The Korean Society of Food Science and Nutrition 2016 Preventive Nutrition and Food Science Vol.21 No.1

The present study was conducted to investigate the effects of beverages containing fermented Akebia quinata extracts on alcoholic hangover. For this study, 25 healthy young men were recruited. All participants consumed 100 mL of water (placebo), commercial hangover beverage A or B, fermented A. quinata leaf (AQL) or fruit (AQF) extract before alcohol consumption. After 1 h, all participants consumed a bottle of Soju, Korean distilled liquor (360 mL), containing 20% alcohol. Blood was collected at 0 h, 1 h, 3 h, and 5 h after alcohol consumption. The plasma alanine transaminase (ALT) activity was highest in the placebo group. Compared with the control group, the AQL and AQF groups showed decreased ALT activity at 5 h after alcohol consumption. Plasma ethanol concentration was increased after alcohol intake and peaked at 3 h after alcohol consumption. Compared with the control group, the A group showed a higher plasma ethanol concentration at 1 h (P<0.05). At 3 h after alcohol consumption, the AQF group showed the lowest mean plasma ethanol concentration compared to the other groups; however, there were no statistical differences. After 5 h of alcohol consumption, the AQL and AQF groups showed lower plasma ethanol concentrations compared with the B group. The sensory evaluation score for the fermented A. quinata fruit extract was lower than for the commercial hangover beverages. In conclusion, the present intervention study results suggest that fermented A. quinata extracts alleviate alcoholic hangover and reduce plasma ethanol concentrations.

다양한 마늘 추출물이 HepG2 세포에서 콜레스테롤 합성에 미치는 효과

정수한(Suhan Jung),이상훈(Sang Hoon Lee),고광석(Kwang Suk Ko) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.12

본 연구에서는 마늘의 다양한 조리법에 따른 냉수추출 마늘추출물이 HepG2 세포 내 콜레스테롤 합성에 미치는 효과에 대하여 알아보고자 세포 내 중성지방 및 콜레스테롤 함량을 확인하고 real-time PCR을 이용하여 작용 기전을 알아보았다. 마늘을 25°C에서 60분간 숙성하였을 경우 1분간 숙성하였을 때와 비교하여 추출물 내 alliin 함량이 감소하는 것을 확인할 수 있었다. 마늘 추출물을 HepG2 세포에 처리하였을 때 세포 내 중성지방 및 콜레스테롤의 함량이 감소하는 모습을 보여주었으며, 특히 동결건조 마늘 추출물이 그 효과가 가장 우수하였다. 콜레스테롤 합성의 제한효소인 HMGCoA reductase의 발현량 역시 마늘 추출물을 처리함에 따라 감소하는 모습을 보였으며, 동결건조 마늘 추출물을 처리하였을 때 가장 많이 감소하는 모습을 보였다. 다만 산성처리를 한 마늘의 추출물을 세포에 처리하였을 때에는 그 효과가 감소하는 것으로 나타났다. 동결건조 마늘 추출물의 농도 비례 효과를 살펴본 결과 동결건조 마늘 추출물의 경우 생마늘을 상온에 60분 숙성시킨 마늘 추출물과 구운마늘 추출물에 비하여 세포 내 중성지방과 콜레스테롤 합성 감소에 효과가 뛰어난 것으로 나타났으며, 이러한 결과는 HMGCoA reducatase mRNA의 상대적 발현 수준 차이 이상의 지질 감소 효과를 나타내는 것을 확인할 수 있었다. 따라서 동결건조 마늘의 경우 생마늘을 섭취하였을 때와 유사한 또는 더 우수한 효능을 보이면서도 오히려 보관성이 우수하고 생마늘 섭취 시 나타날 수 있는 과민 반응 및 알레르기 반응의 위험이 적기 때문에 다양하게 응용될 수 있는 가능성이 있을 것으로 사료되며, 이를 뒷받침할 방안들의 연구가 진행되어져야 할 것으로 판단된다. This study was performed to investigate the effects of various garlic extracts on cholesterol synthesis in HepG2 cells. Raw garlic, grilled garlic, and freeze dried garlic were subjected to cold water extraction, and extracts were incubated at room temperature for 1 min or 60 min. The extracts were treated to HepG2 cells for 4 h, and cholesterol synthesis and mRNA expression level of HMG-CoA reductase were investigated. The alliin contents were reduced when garlic was incubated at room temperature for 60 min. Raw garlic extracts showed lower intracellular cholesterol contents compared to that of the control group. However, raw garlic extracts incubated for 60 min showed no differences compared to the control group. Freeze-dried garlic extract showed minimum intracellular triglyceride and cholesterol contents. Relative mRNA expression level of HMG-CoA reductase, a rate-limiting enzyme of cholesterol synthesis, decreased in the garlic extracts. Compared with 60 min, garlic extracts incubated for 1 min showed a reduced level of HMG-CoA reductase mRNA expression. The freeze-dried garlic extract reduced mRNA expression level of HMG-CoA reductase in a dose-dependent manner in cells treated with 5% of 0.2, 0.5, 0.8, 1.0, and 1.5 mg/mL in medium, and the effect was maxed out at dose of 5% garlic extract at 1.0 mg/mL in medium.

마우스에서 Oleanolic Acid와 Hederagenin이 급성 알코올성 간독성에 미치는 효과

정수한(Suhan Jung),이상훈(Sanghoon Lee),고광석(Kwang Suk Ko) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.3

본 연구는 선행연구를 통한 으름 추출물의 간 보호 효과에 대하여 으름 추출물 내 유효물질로 알려진 oleanolic acid와 hederagenin을 생쥐에 투여하였을 때 알코올로 인한 급성 간독성 상태에서 간 보호 효과를 조사하였다. 그 결과 oleanolic acid와 hederagenin의 투여는 알코올로 유발된 급성 간독성 상태에서 혈중 알코올 농도 감소 및 alcohol dehydrogenase mRNA 발현을 증가시키는 것으로 나타났으며, 간 조직 내 항산화 물질인 glutathione(GSH)의 농도를 증가시키고, GSH synthetic enzyme의 mRNA 발현 역시 증가시키는 것으로 확인되었다. 또한 염증반응 지표인 tumor necrosis factor-alpha의 mRNA 발현도 억제하는 것으로 분석되었다. 위의 결과들과 기존의 실험에서 확인한으름 추출물의 간 보호 효과를 종합하였을 때 으름 추출물의 알코올로 유발된 산화 스트레스, 염증반응에 대한 간 보호 효과는 추출물 내 유효물질로 알려진 oleanolic acid 및 hederagenin의 작용에 의한 것이라 생각한다. We studied the effects of oleanolic acid and hederagenin on acute alcohol-induced hepatotoxicity in mice. Oleanolic acid [10 and 20 mg/kg body weight (BW)/d] or hederagenin (10 and 20 mg/kg BW/d) was orally administered to the study group for 1 week. On the last day of treatment, ethanol (5 g/kg BW) was orally administered to induce acute liver injury. The oleanolic acid-treated group showed lower levels of alanine aminotransferase compared to the ethanol-treated group (EtOH). The mRNA expression level of alcohol dehydrogenase was significantly increased in the high dosage oleanolic acid-treated group compared with the control and EtOH groups. The glutathione levels of the oleanolic acid or hederagenin-treated groups were elevated significantly compared with those of the control and EtOH groups. The mRNA expression levels of glutathione synthetic enzymes were also elevated in the oleanolic acid-treated groups. The oleanolic acid or hederagenin-treated groups also showed lower levels of mRNA expression of tumor necrosis factor alpha. Thus, these results show that oleanolic acid and hederagenin could reduce oxidative stress and hepatotoxicity in ethanol-treated mouse liver.

The agonistic action of URO-K10 on Kv7.4 and 7.5 channels is attenuated by co-expression of KCNE4 ancillary subunit

Jung Eun Lee,Christine Haewon Park,Hana Kang,Juyeon Ko,Suhan Cho,JooHan Woo,Mee Ree Chae,Sung Won Lee,Sung Joon Kim,Jinsung Kim,Insuk So 대한생리학회-대한약리학회 2020 The Korean Journal of Physiology & Pharmacology Vol.24 No.6

KCNQ family constitutes slowly-activating potassium channels among voltage-gated potassium channel superfamily. Recent studies suggested that KCNQ4 and 5 channels are abundantly expressed in smooth muscle cells, especially in lower urinary tract including corpus cavernosum and that both channels can exert membrane stabilizing effect in the tissues. In this article, we examined the electrophysiological characteristics of overexpressed KCNQ4, 5 channels in HEK293 cells with recently developed KCNQ-specific agonist. With submicromolar EC₅₀, the drug not only increased the open probability of KCNQ4 channel but also increased slope conductance of the channel. The overall effect of the drug in whole-cell configuration was to increase maximal whole-cell conductance, to prolongate the activation process, and left-shift of the activation curve. The agonistic action of the drug, however, was highly attenuated by the co-expression of one of the β ancillary subunits of KCNQ family, KCNE4. Strong in vitro interactions between KCNQ4, 5 and KCNE4 were found through Foster Resonance Energy Transfer and co-immunoprecipitation. Although the expression levels of both KCNQ4 and KCNE4 are high in mesenteric arterial smooth muscle cells, we found that 1 μM of the agonist was sufficient to almost completely relax phenylephrine-induced contraction of the muscle strip. Significant expression of KCNQ4 and KCNE4 in corpus cavernosum together with high tonic contractility of the tissue grants highly promising relaxational effect of the KCNQspecific agonist in the tissue.

Hydrogen production by compact combined dimethyl ether reformer/combustor for automotive applications

Jung, Seunghun,Choi, Byungchul,Park, Suhan,Lee, Dong-Weon,Kim, Young Bae Elsevier 2017 International journal of hydrogen energy Vol.42 No.19

<P><B>Abstract</B></P> <P>A computational study of hydrogen production by a dimethyl ether reformer combined with a catalytic combustor is conducted to investigate its feasibility for on-board automotive applications. The combined reactor has a stacked channel structure consisting of alternating reformer monoliths and catalytic combustor monoliths on a heat-conducting substrate. The inner surfaces of the walls of each monolith are coated with reforming and combusting catalysts, respectively. The effects of the feeding flow rate, thermal conductivity of the substrate, and porosity of the catalyst layer on the hydrogen production efficiency of the proposed combined reactor are investigated to determine the optimal design and operating conditions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Compact combined DME reformer for hydrogen production is proposed and computationally studied. </LI> <LI> To achieve the maximum hydrogen yield, proper flow rate ratio between reformer and combustor is required. </LI> <LI> Methanol and carbon monoxide emission is monitored according to various operating conditions. </LI> <LI> Lower thermal conductivity of substrate leads to elevated reactor temperature and better hydrogen yield. </LI> </UL> </P>

기후위기 대응 서울시 ‘그린 뉴딜’ 정책 방향과 추진 전략

유정민(Jung-Min Yu),김고운(KoUn Kim),윤서연(Seoyeon Yoon),주재욱(Jaeuk Ju),김정아(Jeong-Ah Kim),함수한(Suhan Ham) 서울연구원 2021 서울연구원 정책과제연구보고서 Vol.- No.-

The performance of the spiral wound and flat sheet forward osmosis elements with thin film composite membrane

Jeon, Jongmin,Jung, Jaehak,Choi, Joon Young,Kim, Suhan Balaban Publishers 2017 Desalination and Water Treatment Vol. No.

<P>The two different types (flat sheet and spiral wound) of commercial forward osmosis (FO) elements with thin film composite membrane were tested to understand the effects of concentrations and cross-flow velocities of feed solution (FS) and draw solution (DS) on the performance of the element. An FO element tester was customized for reliable measurements of water flux and reverse solute flux during operation time when the concentration difference between DS and FS was decreased. The test results reveal that: (1) the water flux and reverse solute flux increases at higher concentration differences between FS and DS, (2) the higher crossflow velocity increases water flux by decreasing the effects of external concentration polarization, (3) the pressure differential between the inlet and the outlet of the FO element increases at higher crossflow velocities, which may limit the crossflow velocity in a full-scale FO design, (4) the flat sheet element exhibits up to 25 LMH of water flux comparable to the water flux (30-35 LMH) in the coupon tests, and (5) the spiral wound FO element shows a peculiar flux pattern in which the increasing rate of water flux becomes retarded when water flux exceeds a critical value maybe because the DS channel in the FO membrane envelop may not allow a flow rate higher than a critical value due to complexity in the DS flow direction.</P>

낙동강 하구 개방으로 인한 오염된 부산시 상수원 오염 재난 대책

정재학 ( Jaehak Jung ),김민석 ( Minseok Kim ),김수한 ( Suhan Kim ) 한국물환경학회 2017 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2017 No.-

The dynactin subunit DCTN1 controls osteoclastogenesis via the Cdc42/PAK2 pathway

이용덕,김봉준,Suhan Jung,김해민,김민경,권준오,Min-Kyoung Song,이장희,Hong-Hee Kim 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Osteoclasts (OCs), cells specialized for bone resorption, are generated from monocyte/macrophage precursors by a differentiation process governed by RANKL. Here, we show that DCTN1, a key component of the dynactin complex, plays important roles in OC differentiation. The expression of DCTN1 was upregulated by RANKL. The inhibition of DCTN1 expression by gene knockdown suppressed OC formation, bone resorption, and the induction of NFATc1 and c-Fos, critical transcription factors for osteoclastogenesis. More importantly, the activation of Cdc42 by RANKL was inhibited upon DCTN1 silencing. The forced expression of constitutively active Cdc42 restored the OC differentiation of precursors with DCTN1 deletion. In addition, PAK2 was found to be activated by RANKL and to function downstream of Cdc42. The DCTN1-Cdc42 axis also inhibited apoptosis and caspase-3 activation. Furthermore, the antiosteoclastogenic effect of DCTN1 knockdown was verified in an animal model of bone erosion. Intriguingly, DCTN1 overexpression was also detrimental to OC differentiation, suggesting that DCTN1 should be regulated at the appropriate level for effective osteoclastogenesis. Collectively, our results reveal that DCTN1 participates in the activation of Cdc42/PAK2 signaling and the inhibition of apoptosis during osteoclastogenesis.