대두박 수준을 달리한 사료내 미생물 PHYTASE 의 첨가가 잉어의 성장 및 인 배설량에 미치는 영향

김광석(K . S . Kim),김정대(J . D . Kim),송재성(J . S . Song),정관식(K . S . Jeong),우영배(Y . B . Woo) 한국영양사료학회 1996 韓國營養飼料學會誌 Vol.20 No.5

ZNF509S1 downregulates PUMA by inhibiting p53K382 acetylation and p53-DNA binding

Jeon, B.N.,Yoon, J.H.,Han, D.,Kim, M.K.,Kim, Y.,Choi, S.H.,Song, J.,Kim, K.S.,Kim, K.,Hur, M.W. Elsevier Science 2017 Biochimica et biophysica acta. Gene regulatory mec Vol.1860 No.9

Expression of the POK family protein ZNF509L, and -its S1 isoform, is induced by p53 upon exposure to genotoxic stress. Due to alternative splicing of the ZNF509 primary transcript, ZNF509S1 lacks the 6 zinc-fingers and C-terminus of ZNF509L, resulting in only one zinc-finger. ZNF509L and -S1 inhibit cell proliferation by activating p21/CDKN1A and RB transcription, respectively. When cells are exposed to severe DNA damage, p53 activates PUMA (p53-upregulated modulator of apoptosis) transcription. Interestingly, apoptosis due to transcriptional activation of PUMA by p53 is attenuated by ZNF509S1. Thus we investigated the molecular mechanism(s) underlying the transcriptional attenuation and anti-apoptotic effects of ZNF509S1. We show that ZNF509S1 modulation of p53 activity is important in PUMA gene transcription by modulating post-translational modification of p53 by p300. ZNF509S1 directly interacts with p53 and inhibits p300-mediated acetylation of p53 lysine K382, with deacetylation of p53 K382 leading to decreased DNA binding at the p53 response element 1 of the PUMA promoter. ZNF509S1 may play a role not only in cell cycle arrest, by activating RB expression, but also in rescuing cells from apoptotic death by repressing PUMA expression in cells exposed to severe DNA damage.

Enhanced adipogenic differentiation and reduced collagen synthesis induced by human periodontal ligament stem cells might underlie the negative effect of recombinant human bone morphogenetic protein‐2 on periodontal regeneration

Song, D‐,S.,Park, J‐,C.,Jung, I‐,H.,Choi, S‐,H.,Cho, K‐,S.,Kim, C‐,K.,Kim, C‐,S. Blackwell Publishing Ltd 2011 Journal of periodontal research Vol.46 No.2

<P> <I>Song D‐S, Park J‐C, Jung I‐H, Choi S‐H, Cho K‐S, Kim C‐K, Kim C‐S. Enhanced adipogenic differentiation and reduced collagen synthesis induced by human periodontal ligament stem cells might underlie the negative effect of recombinant human bone morphogenetic protein‐2 on periodontal regeneration. J Periodont Res 2011; 46: 193–203. © 2010 John Wiley & Sons A/S</I> </P><P><B>Background and Objective: </B> Recombinant human bone morphogenetic protein‐2 (rhBMP‐2) is a potent inducer for the regeneration of mineralized tissue, but has a limited effect on the regeneration of cementum and periodontal ligament (PDL). The aim of the present study was to determine the effects of rhBMP‐2 on the <I>in vitro</I> and <I>in vivo</I> biologic activity of well‐characterized human PDL stem cells (hPDLSCs) and to elucidate the underlying mechanism of minimal periodontal regeneration by rhBMP‐2.</P><P><B>Material and Methods: </B> hPDLSCs were isolated and cultured, and then transplanted into an ectopic subcutaneous mouse model using a carrier treated either with or without rhBMP‐2. Comprehensive histologic, histometric and immunohistochemical analyses were performed after an 8‐wk healing period. The effects of rhBMP‐2 on the adipogenic and osteogenic/cementogenic differentiation of hPDLSCs were also evaluated. The effect of rhBMP‐2 on both soluble and insoluble collagen synthesis was analyzed, and the expression of mRNA and protein for collagen types I, II, III and V was assessed.</P><P><B>Results: </B> In the present study, rhBMP‐2 promoted both adipogenic and osteogenic/cementogenic differentiation of hPDLSCs <I>in vitro</I>, and the <I>in vivo</I> potential of hPDLSCs to form mineralized cementum and organized PDL tissue was down‐regulated following treatment with rhBMP‐2. Collagen synthesis, which plays a crucial role in the regeneration of cementum and the periodontal attachment, was significantly reduced, with associated modification of the relevant mRNA and protein expression profiles.</P><P><B>Conclusion: </B> In summary, the findings of the present study suggest that enhanced adipogenic differentiation and inhibition of collagen synthesis by hPDLSCs appear to be partly responsible for the minimal effect of rhBMP‐2 on cementum and PDL tissue regeneration by hPDLSCs.</P>

닭의 동품종내 계통간 잡종강세 이용시험

박상문,김동곤,송기덕,오봉국 한국축산학회 1965 한국축산학회지 Vol.7 No.1

To induce hybrid vigor by the reciprocal crossing with SWL (Sung Whan Line), MWL (Minnesota Line), AWL (Dembro Line) and BWL (Derby Line) of single comb white leghorn, 1,330 hens were divided into 16 blacks (Diallal cross with four Line) and fed under condition of N.R.C. feeding standard. In this experiment, hatchability, fertility, mortality, body weight, days required on up to first egg laying date, egg weight, egg quality, winter pauses, intensity, feed utilization, number of eggs layed during the testing period of days and brooding were investigated. Cross breeds showed a little higher fertility and hatchability but there were no significance when their parents had high hatchability. Cross breeds M♀×A♂, M♀×E♂ and A♀×S♂ showed more than ½ decrease in mortality, however cross bred hen showed 2.27% more decrease in mortality than purebreeds. Cross breeds of 6 blocks among 16 blocks at 6 weeks showed significant (P$lt;0.01) difference in body weight. Heaviest cross breed among all blocks were S♀×A♂ and A♀×S♂. Generally adult cross breeds showed heaviest body weight; especially in S♀×A♂ and A♀×S♂ cross breeds showed heaviest body weight among them. Dates requiring on up to first egg laying (50% laying date in the blocks) were showen at cross breeds. Cross breeds shorten 16.42 days than purebreeds and A♀×M♂ was showed shortest clay (171.0 days) in all cross breeds. ♀M×A♂ (175.0 days) and M♀×B♂ (175.0 days) were excellent. Cross breed showed increased egg weight as follows : A♀×S♂ 56.0 g, B♀×M♂ 56.62 g B♀×A♂ 57.40 g and there was significant (P$lt;0.05) difference in egg weight between pure breed and cross breed (M♀×S♂ and B♀×A♂), However, their egg weight was same one as standard (56.0 g) Generally productive block showed light egg weight, because egg weight are related to egg production, Those eggs didn't show any progress in thickness, meat and blood spot. Winter pauses became more short and intensity was more higher than purebreeds. They produced more eggs during winter than purebreeds. Feed utilization was very high in cross breeds. than in purebreeds. The number of egg at 500 days testing period (Hen house) was M♀×B♂ : 201,08, S♀×B♂ : 200.73, B♀×S♂ : 200.21, M♀×S♂ : 197.28 and M♀×A♂ : 191.69 and number of egg per hen in the block was from 230 to 256 in a year. SWL and BWL cross was very excellent in any condition. Generally, we could say that the number of egg was increased by crossing and it showed at 1% level of significance, but cross breeds increased broodiness about 0.56% than did in pure breeds.

1987년 한국에서 발생한 렙토스피라병의 혈청역학적 조사

이증훈,박영수,이우곤,김석용,정선식,우준희,박성광,박경희,송영욱,김선영,기정일,최두혁,강성귀,김주완,최강원,김우열,최명식,최인학,장우현,윤성열 대한감염학회 1988 감염 Vol.20 No.3

Human leptospirosis was an unfamiliar disease in Korea until 1984 that outbreak of leptospirosis occurred among farmers and soldiers after field works for harvesting rice. During that time, Lee and Jo confirmed the first Korean cases of leptospirosis by serological test, isolation of causative agent and autopy findings. Afterward several outbreaks occurred also during autumn especially after flood in every years and some characterisitcs of leptospirosis in Korea such as clinical manifestations, serotypes and seroepidemiological features has been revealed by many investigators. Because of the major mode of transmission between rodents and human is by direct contact with leptospiral urine of rodents or contaminated soil by the urine, leptospirosis in Korea has been primarily a disease of person in occupations heavily exposed to contaminated soil or infected urine such as farmer, army and etc. Therefore it seems that leptospirosis is one of the main communicable diseases to be controlled urgently in Korea, for an agricultural people account for almost half of total Korean people. For clarifying the seroepidemiological patterns of human leptospirosis in Korea by sex, month region and main reacting serovars of L. interrogans among acute febrile disease occurred in 1987, 1,773 patient's sers with acute febrile episodes were tested by microagglutination test using 19 representative strains of leptospiral serogroup as antigen. All of those sera were collected from 10 collaborative clinics located in Kyunggi, Kangwon, Chungbuk, Chungnam, Chonbuk, Chonnam province and Seoul. The results wee summerized as follows. 1) Among 1,773 sera of patients with acute febrile episodes, 219 (12.4%) were seropositive to L. interrogans, 487(27.5%) to R. tsutsugamushi, 241(13.6%) to R.typhi and 160(90.0%) to Hantaan virus. 2) Among seropositives to L.interrogans, the male outnumbered the female, 65% and 35%. 3) For age distribution, 26.9% of seropositives to L.interrogans were fifties, 19.6% were forties, 9.1% were sixties, 5.9% were thirties and 4.1% were twenties. 4) Eighty three percent of seropositives had occurred between September and October in 1987 with a peak in September. 5) Main leptospiral serovars reactive to patient's sera were Icterohaemorrhagiae(54.3%), Canicola(31.0%), CH-48(13.2%), Tarassovi(0.9%)and Cynopteri(0.5%). 6) For regional distribution, 65.8% of seropositives to L.interrogans were residents from Chonbuk, 12.3% were Chonnam, 7.3% were Chungnam, 5.5% were Kyunggi and 1.4% were Kangwon.

The degree complexity of smooth surfaces of codimension 2

Ahn, J.,Kwak, S.,Song, Y. Academic Press 2012 Journal of symbolic computation Vol.47 No.5

For a given term order, the degree complexity of a projective scheme is defined by the maximal degree of the reduced Grobner basis of its defining saturated ideal in generic coordinates (Bayer and Mumford, 1993). It is well known that the degree complexity with respect to the graded reverse lexicographic order is equal to the Castelnuovo-Mumford regularity (Bayer and Stillman, 1987). However, much less is known if one uses the graded lexicographic order (Ahn, 2008; Conca and Sidman, 2005). In this paper, we study the degree complexity of a smooth irreducible surface in P<SUP>4</SUP> with respect to the graded lexicographic order and its geometric meaning. As in the case of a smooth curve (Ahn, 2008), we expect that this complexity is closely related to the invariants of the double curve of a surface under a generic projection. As results, we prove that except in a few cases, the degree complexity of a smooth surface S of degree d with h<SUP>0</SUP>(I<SUB>S</SUB>(2))<>0 in P<SUP>4</SUP> is given by 2+(degY<SUB>1</SUB>(S)-12)-g(Y<SUB>1</SUB>(S)), where Y<SUB>1</SUB>(S) is a double curve of degree (d-12)-g(S@?H) under a generic projection of S. In particular, this complexity is actually obtained at the monomial x<SUB>0</SUB>x<SUB>1</SUB>x<SUB>3</SUB><SUP>(degY<SUB>1</SUB>(S)-12)-g(Y<SUB>1</SUB>(S))</SUP> where k[x<SUB>0</SUB>,x<SUB>1</SUB>,x<SUB>2</SUB>,x<SUB>3</SUB>,x<SUB>4</SUB>] is a polynomial ring defining P<SUP>4</SUP>. Exceptional cases are a rational normal scroll, a complete intersection surface of (2,2)-type, or a Castelnuovo surface of degree 5 in P<SUP>4</SUP> whose degree complexities are in fact equal to their degrees. This complexity can also be expressed in terms of degrees of defining equations of I<SUB>S</SUB> in the same manner as the result of A. Conca and J. Sidman (Conca and Sidman, 2005). We also provide some illuminating examples of our results via calculations done withMacaulay 2 (Grayson and Stillman, 1997).

Evaluation of atmospheric pressure plasma to improve the safety of sliced cheese and ham inoculated by 3-strain cocktail Listeria monocytogenes

Song, H.P.,Kim, B.,Choe, J.H.,Jung, S.,Moon, S.Y.,Choe, W.,Jo, C. Academic Press 2009 FOOD MICROBIOLOGY Vol.26 No.4

The objective of this study was to evaluate the efficacy of atmospheric pressure plasma (APP), which is capable of operating at atmospheric pressure in air, in sliced cheese and ham inoculated by 3-strain cocktail of Listeria monocytogenes (ATCC 19114, 19115, and 19111, LMC). The process parameters considered were input power (75, 100, 125, and 150 W) and plasma exposure time (60, 90, and 120 s). Microbial log reduction increased with increases of input power and plasma exposure time. After 120 s APP treatments at 75, 100, and 125 W, the viable cells of LMC were reduced by 1.70, 2.78, and 5.82 log in sliced cheese, respectively. More than 8 log reductions can be achieved in 120 s at 150 W. In contrast, reductions after 120 s ranged from 0.25 to 1.73 log CFU/g in sliced ham. Calculated D values, the exposure time required to inactivate 90% of a population, from the survival curves of 75, 100, 125, and 150 W of APP treatments were 71.43, 62.50, 19.65, and 17.27 s for LMC in sliced cheese, respectively, and those in sliced ham were 476.19, 87.72, 70.92, and 63.69 s. No viable cells were detected at 125 and 150 W of APP treatment in sliced cheese, irrespective of plasma exposure time, after 1 week at a detection limit of 10<SUP>1</SUP> CFU/g. These results indicate that the inactivation effects of APP on L. monocytogenes are strongly dependent on the type of food.

급속응고한 고망간 합금강 박판의 미세조직 및 기계적 성질에 미치는 V 첨가원소 및 열처리 영향

김형수,조성석,최창범,천병선 대한금속재료학회(대한금속학회) 1990 대한금속·재료학회지 Vol.28 No.2

We have investigated the effects of the microstructure and mechanical properties with V content and heat treatment on the rapidly solidified V added Fe-13 Mn-1.2C steel strips which were produced by a twin roll melt-quenching method. Regardless of the V content, solidification process typically took place by primary austenite formation and then the residual liquid phase formed austenite by massive transformation. In melt-quenched, the austenite to ferrite transformation was suppressed, resulting in only austenite. As the V content increased, the equiaxed structure region increased and the secondary dendrite arm spacing became a little wider. With the increase in V content, the refining of austenite grain size after heat treatment was accelerated because the increase in the formation of carbides suppressed the grain growth. As 0.2%Y.S. was increased with increasing V content, a relation between it and grain size as a function of V content can be expressed as the following equation. 0.2% Y.S.(㎏/㎟)=22.05+7.4/√D The mechanism of the large strengthening effect of V on the 0.2% Y.S. was considered to be attributable to the grain refining by V addition and the large increase of Petch constan

복합재료 특성 평가 1 : 다축 구조 S-2 유리섬유 복합재의 충격 특성

송승욱 ( S. W. Song ),이창훈 ( C. H. Lee ),변준형 ( J. H. Byun ),황병선 ( B. S. Hwang ),엄문광 ( M. K. Um ),이상관 ( S. K. Lee ) 한국복합재료학회 2005 한국복합재료학회 학술발표대회 논문집 Vol.- No.1

식품중의 수산분석법에 관한 연구

김복성,이영자,홍기형,이창희,류시생,진희숙,송성실,김용철,이명환 국립보건연구원 1992 국립보건원보 Vol.29 No.2