Ultrasonic Degradation of Endocrine Disrupting Compounds in Seawater and Brackish Water

So Young Park,Jong Sung Park,Ha Yoon Lee,Ji Yong Heo,Yeo Min Yoon,Kyung Ho Choi,Nam Guk Her 대한환경공학회 2011 Environmental Engineering Research Vol.16 No.3

In this study, a series of experiments was conducted on the relative degradation of commonly known endocrine-disrupting compounds such as bisphenol A (BPA) and 17α-ethinyl estradiol (Ee₂) in a single-component aqueous solution using 28 and 580 kHz ultrasonic reactors. The experiments were conducted with three different types of model water: deionized water (DI), synthetic brackish water (SBW), and synthetic seawater (SSW) at pH 4, 7.5, and 11 in the presence of inert glass beads and humic acids. Significantly higher sonochemical degradation (93-97% for BPA) occurred at 580 kHz than at 28 kHz (43-61% for BPA), regardless of water type. A slightly higher degradation was observed for Ee₂ compared to that of BPA. The degradation rate of BPA and Ee₂ in DI water, SBW, and SSW after 30 min of ultrasound irradiation at 580 kHz increased slightly with the increase in pH from 4 (0.073-0.091 min-1 for BPA and 0.081-0.094 min-1 for Ee₂) to 7.5 (0.087-0.114 min-1 for BPA and 0.092?0.124 min-1 for Ee₂). In contrast, significant degradation was observed at pH 11 (0.149-0.221 min-1 for BPA and 0.147-0.228 min-1 for Ee₂). For the given frequencies of 28 and 580 kHz, the degradation rate increased in the presence of glass beads (0.1 mm and 25 g) for both BPA and Ee₂: 0.018-0.107 min-1 without beads and 0.052-0.142 min-1 with beads for BPA; 0.021-0.111 min-1 without beads and 0.054-0.136 min-1 with beads for Ee₂. A slight increase in degradation of both BPA and Ee₂ was found as the concentration of dissolved organic carbon (DOC, humic acids) increased in both SBW and SSW: 0.107-0.115 min-1 in SBW and 0.087-0.101 min-1 in SSW for BPA; 0.111-0.111 min-1 in SWB and 0.092-0.105 min-1 in SSW for Ee₂. After 30 min of sonicating the humic acid solution, DOC removal varied depending on the water type: 27% (3 mg L-1) and 7% (10 mg L-1) in SBW and 7% (3 mg L-1) and 4% (10 mg L-1) in SSW.

IGCC용 고온건식 탈화제의 반응속도 모사에 관한 연구

박소진,이영우,민세홍,나재익,위영호 忠南大學校 産業技術硏究所 1999 산업기술연구논문집 Vol.14 No.1

고온건식 탈황공정에서 탈황제의 황화, 재생 반응은 비촉매 기고 반응이다. 이러한 비촉매 기고 반응의 수학적 모사는 실험데이터의 설명, 공정의 설계 그리고 scale-up에 있어서 매우 중요한 정보가 된다. 탈황제의 황화, 재생 반응에는 spray dryer로 제조된 zinc titanate 탈황제를 사용하였고, 변형된 미반응핵 모델식에 적용하여 각 반응의 반응속도를 해석한 결과 탈황제의 황화반응에는 화학반응 저항과 고체 상태의 확산 저항이 회합하여 전체반응속도를 지배하며, 이때 반응속도상수는 367.67cm/min 이었고 고체상태의 확산계수는 29.66cm²/min이었다. 또한 재생반응시에 는 화학반응 저항만이 전체반응의 저항으로 관여하며, 이때의 반응속도상수는 3.45x10e cm/min이었다. In the dry-type high temprature gas desulfurization process, sulfidation and regeneration reactions of desulfrrization sorbents are noncatalytic gas-solid reactions. The mathematical modeling of noncatalytic gas-solid reactions is improtant in order to interpret laboratory data and in design and scale-up. Zinc titanate sorbent to manufacture by spray dryer is used in sulfidation and regeneration reactions of desulfuization sorbents. The result which analyzes reaction rate of sulfidation reaction by applying each reactions to modified unreacted core model is that the global reaction rate is controlled by associating with chemical reaction resistance and solid state diffusion resistance. In this case,reaction rate constant is367.67e cm/min and solid state diffusion coefficient is 29.66e cm²/min Also gloval reaction rate of regeneration reaction is controlled only by chemical reaction resistance. In this case, reaction rate constant is 3.45x10e cm/min.

Lactobacillus pentosus 발효에 의한 담쟁이덩굴 줄기 추출물의 항산화 및 세포보호 효과

박소현 ( So Hyun Park ),성준섭 ( Joon Seob Seong ),이건수 ( Keon Soo Lee ),박영민 ( Young Min Park ),현송화 ( Song Hua Xuan ),차미연 ( Mi Yeon Cha ),강희철 ( Hee Cheol Kang ),박수남 ( Soo Nam Park ) 대한화장품학회 2017 대한화장품학회지 Vol.43 No.3

본 연구에서는 담쟁이덩굴 줄기 70% 에탄올 추출물과 발효균주 Lactobacillus pentosus를 이용하여 발효시킨 담쟁이덩굴 줄기 발효추출물에 대하여 항산화 및 세포보호 효과를 측정하였다. 1,1-Diphenyl-2-picrylhydrazyl (DPPH)를 이용한 자유라디칼 소거 활성(FSC₅₀)은 담쟁이덩굴 줄기 추출물 및 발효추출물이 각각 42.3 및 34.5 μ g/mL로 발효 후의 라디칼 소거활성이 약 18.4% 더 높게 나타났다. Lumiol-의존성 화학발광법을 이용한 Fe³⁺-EDTA/H₂O₂계에서의 총 항산화능(OSC₅₀) 평가에서도 담쟁이덩굴 줄기 추출물과 발효추출물은 각각 2.6 및 2.5 μ g/mL로 발효 후가 약 4.2% 정도 더 높은 총 항산화능을 나타냈다. ¹O₂로 유도된 적혈구 세포손상에 있어서 추출물 및 발효추출물의 세포 보호 효과(τ₅₀)는 50 μ g/mL에서 각각 126.4 및 173.0 min을 나타내어 발효 후 세포 보호 효과가 약 34.0% 더 높게 나타났다. 발효추출물은 지용성 항산화제로 알려진 (+)-α-tocopherol (43.4 min)보다도 3.9배 높은 세포 보호 활성을 보여주었다. 사람 섬유아세포인 Hs68을 대상으로 elastase 저해 활성을 조사하였다. Elastase 저해 활성(IC₅₀)은 담쟁이덩굴 줄기 추출물과 발효추출물에서 각각 873.6 및 687.8 μ g/mL로 발효 후에 elastase 저해 활성이 약 21.3% 더 높은 것으로 나타났다. 이상의 결과들은 담쟁이덩굴 줄기 발효추출물이 항산화 작용과 더불어 주름개선 효과를 가지는 천연 화장품 소재로써 응용 가능성이 있음을 시사한다. In this study, the antioxidant activities, cellular protective effects, and inhibitory effects on elastase of non-fermented and fermented extracts of Parthenocissus tricuspidata (P. tricuspidata) stem using Lactobacillus pentosus were investigated. The free radical scavenging activities (FSC₅₀) of non-fermented and fermented extracts were 42.3 and 34.5 μg/mL, respectively, in which the activity after fermentation was approximately 18.4% higher. Reactive oxygen species (ROS) scavenging activities (OSC₅₀) in Fe³⁺-EDTA/H₂O₂ system of non-fermented and fermented extracts were 2.6 and 2.5 μg/mL, respectively. The activity after fermentation was approximately 4.2% higher. In the ¹O₂-induced cellular damage of erythrocytes, the cellular protective effects (τ₅₀) of non-fermented and fermented extracts were 126.4 and 173.0 min at 50 μg/mL, respectively. The activity after fermentation was approximately 34.0% higher. The effect of fermented extract was 3.9 times higher than (+)-α-tocopherol (τ₅₀ = 43.4 min), known as a lipophilic antioxidant at 50 μg/mL. The inhibitory effect of elastase was investigated to predict the anti-wrinkle efficacy using Hs68 human fibroblasts cells. The elastase inhibitory activities (IC₅₀) of non-fermented and fermented extracts were 873.6 and 687.8 μg/mL, respectively, and the activity after fermentation was approximately 21.3% higher. These results indicated that fermented extract of P. tricuspidata stem has potentials as natural cosmetic ingredients with antioxidant and anti-wrinkle effect.

Effect of ER signal peptides (KDEL) on expression and function of anti-virus monoclonal antibody in plant

Da-Young Park,Yangkang So,Kyung-Jin Lee,Zhe Lu,Eun-Yi Oh,Kwang-Wok Min,Seul-Ki Lee,Jung-Hwan Lee,Mi-Hyun Ahn,Mira Song,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering has led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current antibody expression systems. In this study, the expression levels of mAbP SO57 with or without ER-signal peptides (Lys-Asp-Gly-Leu;KDEL) in transgenic tobacco plants were analysed in transgenic plant. PCR and Reverse Transcription-PCR analyses showed existence of heavy and light chain genes of mAb with or without KEDL and their transcription in plant, respectively. Western blot showed that the expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL. Flow cytometry analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb have similar binding activity to the FcγRI receptor. High-performance liquid chromatography analysis showed that the mAbP SO57 with KDEL had glycan profile with both oligomannose and golgi type, whereas the mAbP SO57 without KDEL had only golgi type glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest that the potential of mAbP SO57 for rabies immunotherapy is regardless of plant specific glycan structures.

Complete genome sequence of Sphingobium sp. strain PAMC 28499 reveals a potential for degrading pectin with comparative genomics approach

So‑Ra Han,Sung‑Min Jang,Young Min Chi,Byeollee Kim,정상희,Yung Mi Lee,Jun Uetake,Jun Hyuck Lee,Hyun Park,오태진 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background Spingobium sp. PAMC 28499 is isolated from the glaciers of Uganda. Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor. The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry. Objective In this study, complete genome sequence of Spingobium sp. PAMC 28499 and comparative analysis of Spingobium species strains isolated from variety of the region. Methods Genome sequencingwas performed using PacBio sequel single-molecule real-time (SMRT) sequencing technology. The predicted gene sequences were functionally annotated and gene prediction was carried out using the program NCBI nonredundant database. And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes). Results The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons. Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases. CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae. These results confrmed that this Sphingobium sp. strain PAMC 28499 have similar patterns to RG I pectin-degrading pathway. Conclusion In this study, isolated and sequenced the complete genome of Spingobium sp. PAMC 28499. Also, this strain has comparative genome analysis. Through the complete genome we can predict how this strain can store and produce energy in extreme environment. It can also provide bioengineered data by fnding new genes that degradation the pectin

다중지능이론을 적용한 디자인 교육활동이 유아의 지능에 미치는 영향

박소연(Park So Yeon),송민정(Song Min Jung) 한국디지털디자인협의회 2012 디지털디자인학연구 Vol.12 No.4

현대사회는 탁월한 성취, 창의성 등을 두루 갖춘 창조적인 인재상을 추구한다. 이에, 창의적 인재양성을 위한 창의적 교수학습법이 화두가 되고 있다. 창의적 교수학습법의 일환으로 창의성, 자율성, 개별화 교육, 자기주도적 학습 등이 핵심 키워드로 거론되고 있으며, 이는 획일주의 교육에서 벗어나 다차원적 관점에서 바라보는 다양한 교육방법의 필요성과 중요성이 있음을 시사하고 있다. 이에 본 연구에서는 개인차 고려, 통합교과과정 적용, 학습자중심 교육을 지향하는 다중지능이론을 적용한 교수학습안을 설계하고, 학습자에게 적용 후, 학습자에게 미치는 영향에 대해 알아보고자 한다. 다중지능이론은 Howard Gardner박사가 정립한 것으로, 인간의 지능은 총 8가지 언어지능, 논리수학지능, 시각공간지능, 자연탐구지능, 개인내적지능, 대인관계지능, 음악지능, 운동지능 등으로 측정?평가할 수 있다고 한다. 그는 다중지능이론을 통해 획일화된 교육이 아닌 개인차를 고려한 학습자중심 교육의 접근법이 이루어져야 한다고 주장한다. 특히 유아의 다중지능 특성을 유아기 초기부터 발견하고 이에 적합한 개별교육이 수행된다면 유아의 흥미와 자아를 존중하는 전인교육이 수행되는 것이며 이러한 교육을 받은 유아들은 미래 사회에서 자신만의 쟁력을 갖춘 성인으로 성장할 수 있다고 지적하고 있다.1)유아교육과정의 원리요소 미루어 보아 놀이 개념의 디자인 중심 학습활동이 유아의 흥미 및 동기유발, 통합적 교과과정의 원리 측면에서 효율적인 교육적 도구가 될 수 있다. 이에 본 연구는 다중지능이론에 근거한 유아 디자인프로그램 및 교수학습안을 만들어 프로그램 적용 전?후의 결과를 비교함으로써 첫째, 유아의 디자인 교육의 필요성과 중요성에 대해 알아보고, 둘째, 다중지능이론을 적용한 디자인프로그램이 유아의 각 지능에 미치는 영향에 대해 살펴보고자 한다. In the modern society, a variety of sciences and technologies are fused to create new knowledge and value. Because of it, a creative teaching-learning method is continuously mentioned for cultivation of creative talent. Recently, creativeness, approach centered on learners and self-directed learning all of which are being emphasized in educational circles strongly indicate that each individual needs to aggressively introduce their own multiple intelligence profiles for teaching-learning process. It is the very Multiple Intelligence Theory developed by Howard Gardner that has suggested a new direction for education. He not only defined the human intelligence as standardized and single intelligence from the cognitive viewpoint but also categorized it into the total 8 fields intelligences. Howard Gardener points out that if the nature of children’s multiple intelligence can be found from early childhood and the individual education appropriate to it is to be carried out the so-called whole-rounded education respecting their interest and egos will be accomplished and those children receiving the education will grow to adults with their own competitiveness in the future. Through design education among children’s learning domains, children can find out the designs which are everywhere in our daily lives, explore the possibility of various expressions and have both convergent and divergent thoughts. Accordingly, the design education is an excellent teaching medium to make children expand the width of thought and cultivate the logic thought. Thus, for this study, a program for children’s design activities by applying Multiple Intelligence Theory will be made to figure out what influence it will have on children’s multiple intelligences.

합성동사의 관용 해석과 문법 처리 문제에 대한 소고

박소영(Park, So-young),이정민(Lee, Jeong-Min) 한국어학회 2021 한국어학 Vol.93 No.-

이 논문은 관용적 의미를 갖는 ‘-어’형 합성동사의 형성 및 문법 처리 문제에 대하여 새로운 접근 방식을 제안하는 것을 목적으로 한다. 어휘론적 관점에 따르면 합성동사는 어휘부에 등재된, 통사부의 원자적 입력 단위에 해당한다. 그러나 이들은 다양한 측면에서 분리 가능하여, 이른바 어휘 충실성을 위배한다. 이 논문은 분산형태론 관점에서 합성동사를 두 개의 어근이 국면 핵인 v에 결합하는 구조를 이루는 것으로 분석한다. 해당 구조는 통사부 이후 개념접합부에서 하나의 단일한 의미해석 단위를 이루어 비합성적 의미해석의 가능성을 갖게 된다. 이러한 분석은 합성동사가 관용적인 의미해석을 가짐에도 불구하고 분리 가능한, 외견상 모순적인 속성을 자연스럽게 설명해 줄 수 있다. This paper, from the perspective of Distributed Morphology, addresses how to explain idiomatic interpretations of compound verbs in Korean. In terms of the Lexicalist framework, compound verbs are supposed to be syntactic atoms listed in the lexicon. Contrary to this premise, they exhibit the characteristics that are separable in various ways, thus apparently violating the so-called Lexical Integrity Hypothesis. This paper argues that a compound verb has a structure in which a Root phrase consisting of two verb roots is combined with a v, thus forming a single phasal structure. Due to this structure, it may behave as a single unit for a semantic interpretation search implemented after the syntactic operation, so that it can receive an idiomatic reading. According to this analysis, the apparent contradiction that compound verbs are idiomatic in meaning but nevertheless are separable can naturally fall into place.

사상성격검사(SPQ)의 한의학적 활용 현황에 대한 체계적 문헌 분석

김민성 ( Min Seong Kim ),황요순 ( Yosun Hwang ),박소정 ( So Jung Park ),이준희 ( Junhee Lee ),김종우 ( Jongwoo Kim ),이수진 ( Soo Jin Lee ),채한 ( Han Chae ) 대한한방신경정신과학회 2021 동의신경정신과학회지 Vol.32 No.3

Objectives: Operational definition and its validated measure of traditional Korean medicine (TKM) are pivotal for research studies and reliable clinics. There have been ten years of clinical studies on Eum-Yang (Yin-Yang) and Sasang typology using Sasang Personality Questionnaire (SPQ) of integrative biopsychology. The objective of this study was to perform a systematic review on the use of SPQ to provide insights and foundation for its further studies and clinical application. Methods: Clinical studies on SPQ were searched using five domestic and foreign research databases. Attained research articles were then subjected to predefined systematic reviews. Demographic characteristics of participants and research topics of articles were described. Psychological and physical features of SPQ and its subscales were reviewed and compiled to reveal biopsychological characteristics. Results: There were 33 articles with 15,085 participants on Eum-Yang (n=19) and Sasang typology (n=14). The SPQ-total score was in an increasing order of So-Eum, Tae-Eum, and So-Yang. Eum-Yang groups were categorized at a 3:4:3 ratio using %ile score of SPQ-total. The SPQ-total showed a significant positive correlation with Novelty-Seeking and a negative correlation with Harm-Avoidance in psychological perspective, while it was not significantly correlated with Body Mass Index or Ponderal Index in physical perspective. The SPQ-total and subscales explained psychopathological characteristics of stress recognition, negative emotion, problem behavior, and depression. Conclusions: The SPQ was found to be an objective biopsychological measure of TKM for Sasang typology and psychopathology. It would provide foundation for ‘Korean Psychology’ and multidisciplinary studies.

연구 논문 : 전기화학적 마이크로머시닝 기술을 이용한 균일한 니오븀 표면 에칭 연구

김경민 ( Kyung Min Kim ),유현석 ( Hyeon Seok Yoo ),박지영 ( Ji Young Park ),신소운 ( So Woon Shin ),최진섭 ( Jin Sub Choi ) 한국공업화학회 2014 공업화학 Vol.25 No.1

본 연구에서는 micro-contact printing을 통하여 니오븀 호일 표면 위에 균일한 에칭 pits를 형성하였다. 균일한 보호층을 형성하고자 전해연마의 효과를 확인하였으며, 기존의 O2 플라즈마 공정 없이 손쉽게 균일한 에칭 pits를 형성시킬 수 있는 조건을 확인하였다. 메탄올 혼합 전해질을 사용하여 10 min 동안 에칭을 진행한 결과 니오븀 호일 표면 위에 지름과 간격이 각각 10 μm와 5 μm로 잘 정렬된 에칭 pits를 관찰하였다. We describe the preparation of highly-ordered etching pits on the Nb foil through a micromachining. The effects of electro-chemical polishing on the formation of uniformly-patterned protective epoxy layer was investigated. Unlike the previous proc-ess using O2 plasma, well-ordered etched pits were prepared without any dry processes. As a result, the Nb foil with the well-ordered pits of 10 μm × 5 μm could be obtained by electrochemical etching in methanolic electrolytes for 10 min.

5종의 치주질환균에 대한 프로폴리스의 항균효과

이은지,김소민,박소민,박복리,홍성익,김현정 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

Since the oral cavity is directly connected to the outside, it is an important site for microbial invasion, and since it is an environment that is nutritionally and physiologically easy to multiply bacteria, many bacteria always form bacteriological flora in the oral cavity. And propolis is a natural treatment used in many health food stores in various forms for local treatment. This study analyzes the changes in the activity of five oral microorganisms before and after consumption of propolis for various age groups to find out how propolis, which shows the efficacy of anti-inflammatory, antibacterial, and immune sensitization, affects oral microorganisms. Five types of bacteria possessed by periodontal disease patients were specified and digitized targeting the public person, and then before and after propolis intake was compared, and the levels of periodontal disease bacteria in the intake group and the non-consumption group were compared. As a result of comparing the patterns before ingestion (0 week) and after ingestion (3 week) in the oral bacteria analysis, it was confirmed that the bacteria decreased overall except for the five populations in the propolis intake group. In contrast, in the comparative group that did not intake propolis, the bacteria of five periodontal disease increased except for the four populations. What can be seen from this result is that propolis is effective in reducing bacteria in five types of periodontal disease. In order to use propolis as a food for preventing periodontal disease and to get a better effect, further research is needed to find out the mechanism of antibacterial action in propolis compounds.