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Exploring broad-spectrum and durable disease resistance genes for rice improvement
Shiping Wang 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Fungal blast caused Magnaporthe oryzae, bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo), bacterial streak caused by Xanthomonas oryzae pv. oryzicola (Xoc) are devastating diseases of rice worldwide. Application of host resistance to these pathogens is the most economical and environment-friendly approach to solve this problem. Some major disease resistance (MR) genes controlling qualitative resistance and quantitative trait loci (QTLs) controlling quantitative resistance are valuable sources for broad-spectrum and durable disease resistance. We have characterized a number of rice MR genes and resistance QTL genes that confer a broad-spectrum or durable resistance to M. oryzae, Xoo, and Xoc. How to efficiently use these genes for rice improvement will be discussed.
A Dominant Mutation in ARL2 Causes Impaired Adventitious Root Development in Rice
Shiping Liu,Yanhong Xue,Xiaofei Wang,Botao Zhang,Yuting Bi,Min Qiu,Guangxi Wang,Ping Wu 한국식물학회 2011 Journal of Plant Biology Vol.54 No.4
Adventitious roots are vital for water and nutrient assimilation by cereal crops because they comprise the bulk of the fibrous root system. We isolated and analyzed a rice mutant, adventitious rootless 2 (arl2), which failed to initiate adventitious root primordia during early development. Its seminal root produced fewer lateral roots than from the wild type. This mutant also exhibited pleiotropic phenotypes of longer and thicker seminal roots, a different morphology for the first leaf, delayed heading, and a greater tiller angle. Physiological experiments showed that exogenous auxin and ethylene could rescue adventitious root growth, a response opposite that for two previously reported mutants, arl1 and gnom1. Activity in the auxin signal pathway and the polar auxin transport system was normal for arl2. Compared with the wild type, arl2 plants showed enhanced sensitivity to ethephon but decreased sensitivity to AgNO_3, an inhibitor of ethylene. Genetics analysis demonstrated that this mutant is controlled by a single dominant gene; ARL2 was mapped within a 100-kb interval on the short arm of chromosome 2.
Huifang Wang,Shiping Liang,TaoMa,Qiang Xiao,Panrong Cao,Xuan Chen,Wenquan Qin,Hongpeng Xiong,Zhaohui Sun,XiujunWen,Cai Wang 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.2
Ectropis grisescens Warren (Lepidoptera: Geometridae) is an important defoliator pest of the tea plant, Camellia sinensis L. Our previous studies showed that E. grisescens pupae had significantly lower body water content when they pupated in the dry soil (20%-moisture) than those that pupated in the wetter soil (50%- or 80%-moisture). However, it is still unclear whether low-moisture conditions during pupating would further influence the physiological and fitness traits of adults. In the present study, E. grisescens individuals were allowed to pupate in 20%-, 50%- or 80%-moisture soil (silt loam), or in containers without soil (no-substrate conditions) in the laboratory. No significant difference in emergence was detected among different pupating conditions. However, female moths that emerged from 20%-moisture soil had significantly lower body water content compared to 80%-moisture soil, whereas the body water content of males was similar among the four treatments. The paired moths that emerged from 20%-moisture soil laid significantly fewer eggs than those that emerged from 50%- moisture soil. In addition, the number of hatched offerings (larvae) were significantly less when the adults previously pupated in no-soil containers compared to 50%-moisture soil. The longevity of adults was shortest when they emerged from 20%-moisture soil. Our study showed that the no-substrate and low-moisture conditions during pupating adversely affected E. grisescens adults.
Mengqi Wang,Kaiyue Zhang,Feixiang Ji,Yurong Guo,Chao Wang,Shiping Wang,Ya Chu,Guangjiu Zhao 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.104 No.-
A water-soluble probe composite material (CDs/ZnO) was synthesized by microwave pyrolysis methodsof citric acid, urea and zinc oxide (ZnO) with aggregation-induced emission activity. We creatively realizedthe composite of CDs and nano-ZnO in the form of foam, then used as bactericide for the first timeand showed excellent antibacterial properties. Also, the composite materials overcame the problem ofaggregation-induced quenching (ACQ) and realized solid-state emission. The materials had two differentemission centers, and the two emission peaks were varied with the change of excitation wavelength. Furthermore, Methyl red (MR) showed fluorescence quenching for CDs/ZnO with obvious linear correlation,which demonstrated potential in the measurement of MR in dyeing wastewater. The CDs/ZnO weobtained perfectly inherited the advantages of CDs and nano ZnO, it has not just no cytotoxicity to cells,but also has a good killing effect on bacteria. Hence, this work shows significant adaptable fluorescencebasedprotocol with enormous potential applications in biology.
Overexpression of OsVP1 and OsNHX1 Increases Tolerance to Drought and Salinity in Rice
Shiping Liu,Luqing Zheng,Yanhong Xue,Qian Zhang,Lu Wang,Huixia Shou 한국식물학회 2010 Journal of Plant Biology Vol.53 No.6
Drought and salinity are major abiotic stresses affecting rice production. To improve plant tolerance to salinity and drought, we overexpressed rice Na+/H+ exchangers (OsNHX1) and H+-pyrophosphatase in tonoplasts (OsVP1) in a japonica elite rice cultivar, Zhonghua 11. Compared with our wild-type control, transgenic plants overexpressing both genes incurred less damage when exposed to long-term treatment with 100 mM NaCl or water deprivation. Under high-saline conditions, the transformants accumulated less Na+ and malondialdehyde in the leaves,thereby allowing the plants to maintain a low level of leaf water potential and reduce stress-induced damage. Those transgenics also had higher photosynthetic activity during the stress period. Under those conditions, they also showed an increase in root biomass, which enabled more water uptake. These results suggest that OsVP1 and OsNHX1 improve the tolerance of rice crops against drought and salt by employing multiple strategies in addition to osmotic regulation.
TaoMa,Huifang Wang,Shiping Liang,Qiang Xiao,Panrong Cao,Xuan Chen,Ying Niu,Yurong He,Zhaohui Sun,XiujunWen,Cai Wang 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.1
Ectropisgrisescens Warren (Lepidoptera: Geometridae) is one of the most severe defoliating pests of tea plants. Synthetic pesticides have been widely applied to control this pest in tea plantations, but pesticide residues may decrease the quality and safety of tea products. In the present study, we hypothesized that soil treatment with the two commercial biopesticides (Shuiguxin ® ) based on Metarhizium anisopliae (Metchnikoff) Sorokin and Beauveria bassiana (Balsamo) could reduce the survivorship and fitness of E. grisescens. Wandering larvae of E. grisescens were allowed to pupate in soil treated with each biopesticide, and the concentrations of M. anisopliae (Shuiguxin ® ) and B. bassiana (Shuiguxin ® ) that produced the 50% mortality values (LC 50 ) were 2.9 × 10 6 and 1.6 × 10 7 conidia/g soil, respectively. Artificial burying the pupae using soil treated with M. anisopliae (Shuiguxin ® ) and B. bassiana (Shuiguxin ® ) (1 × 10 8 or 1 × 10 9 conidia/g soil for both biopesticides) also significantly reduced emergence success of E. grisescens. In addition, choice tests showed that soil treated with the high concentration of M. anisopliae (Shuiguxin ® ) or B. bassiana (Shuiguxin ® ) had repellent effects on pupating E. grisescens. However, sublethal concentrations (LC 25 and LC 50 ) of both biopesticides did not significantly affect fecundity, fertility and longevity of post-emerged adults. Our study showed that soil treatment with the two commercial biopesticides caused direct mortality of pupating E. grisescens, but may not effectively suppress E. grisescens populations at sublethal concentrations. The realistic application of the fungal dosage in fields should be determined in future studies
A dynamic finite element method for the estimation of cable tension
Yonghui Huang,Quan Gan,Shiping Huang,Ronghui Wang 국제구조공학회 2018 Structural Engineering and Mechanics, An Int'l Jou Vol.68 No.4
Cable supported structures have been widely used in civil engineering. Cable tension estimation has great importance in cable supported structures’ analysis, ranging from design to construction and from inspection to maintenance. Even though the Bernoulli-Euler beam element is commonly used in the traditional finite element method for calculation of frequency and cable tension estimation, many elements must be meshed to achieve accurate results, leading to expensive computation. To improve the accuracy and efficiency, a dynamic finite element method for estimation of cable tension is proposed. In this method, following the dynamic stiffness matrix method, frequency-dependent shape functions are adopted to derive the stiffness and mass matrices of an exact beam element that can be used for natural frequency calculation and cable tension estimation. An iterative algorithm is used for the exact beam element to determine both the exact natural frequencies and the cable tension. Illustrative examples show that, compared with the cable tension estimation method using the conventional beam element, the proposed method has a distinct advantage regarding the accuracy and the computational time.
Jiping Liu,Shuyuan Wang,Liang Feng,Dongying Ma,Qiang Fu,Yu Song,Xiaobin Jia,Shiping Ma 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.7
Diabetic encephalopathy (DE) is one of the severe complications in patients with diabetes mellitus. Paeonol, an active compound isolated from the root bark of Paeonia suffruticosa, has significant antidiabetic activity in vivo. However, its underlying beneficial effects on DE were unclear. In the present study, the protective activity of paeonol on DE was evaluated in streptozotocin (STZ)-induced diabetic rats. Paeonol at 50 and 100mg/kg significantly increased body weight and decreased blood glucose levels, glycosylated serum proteins, and serum advanced glycation end products (AGEs) levels. Immunohistochemistry assays and Western blot analysis revealed a significant decrease in expressions on receptor for advanced glycation end products (RAGE) and nuclear factor kappa B (NF-jB) in hippocampus and cerebral cortical neurons after paeonol treatment. Furthermore, paeonol significantly increased glutathione content and remarkedly decreased induced nitric oxide synthase activity in hippocampus tissue. Our findings indicated that paeonol could improve the pathological damage of DE in STZ-induced diabetic rats. It might be associated with the modulating AGEs/RAGE/NF-jB pathway. This study suggested that paeonol might be a promising candidate for the prevention and treatment of DE.