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SEO, HYE-SOOK,KU, JIN MO,CHOI, HAN-SEOK,CHOI, YOUN KYUNG,WOO, JONG-KYU,KIM, MINSOO,KIM, ILHWAN,NA, CHANG HYEOK,HUR, HANSOL,JANG, BO-HYOUNG,SHIN, YONG CHEOL,KO, SEONG-GYU D.A. Spandidos 2016 ONCOLOGY REPORTS Vol.36 No.1
<P>Flavonoids are assumed to exert beneficial effects in different types of cancers at high concentrations. Yet, their molecular mechanisms of action remain unknown. The present study aimed to examine the effect of quercetin on proliferation and apoptosis in HER2-expressing breast cancer cells. The anti-proliferative effects of quercetin were examined by proliferation, MTT and clonogenic survival assays. The effect of quercetin on expression of apoptotic molecules was determined by western blotting. Luciferase reporter assay was performed to measure signal transducer and activator of transcription 3 (STAT3) transcriptional activity. ELISA assay was performed to measure intracellular MMP-9 levels. Immunocytochemistry was performed to evaluate the nuclear STAT3 level. The results revealed that quercetin inhibited the proliferation of BT-474 cells in a dose- and time-dependent manner. Quercetin also inhibited clonogenic survival (anchorage-dependent and -independent) of BT-474 cells in a dose-dependent manner. These growth inhibitions were accompanied with an increase in sub-G<SUB>0</SUB>/G<SUB>1</SUB> apoptotic populations. Quercetin induced caspase-dependent extrinsic apoptosis upregulating the levels of cleaved caspase-8 and cleaved caspase-3, and inducing the cleavage of poly(ADP-ribose) polymerase (PARP). In contrast, quercetin did not induce apoptosis via intrinsic mitochondrial apoptosis pathway since this compound did not decrease the mitochondrial membrane potential and did not affect the levels of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (BAX). Quercetin reduced the expression of phospho-JAK1 and phospho-STAT3 and decreased STAT3-dependent luciferase reporter gene activity in the BT-474 cells. Quercetin inhibited MMP-9 secretion and decreased the nuclear translocation of STAT3. Our study indicates that quercetin induces apoptosis at concentrations >20 <I>µ</I>M through inhibition of STAT3 signaling and could serve as a useful compound to prevent or treat HER2-overexpressing breast cancer.</P>
Seo, Jang-Kyun,Ohshima, Kazusato,Lee, Hyeok-Geun,Son, Moonil,Choi, Hong-Soo,Lee, Su-Heon,Sohn, Seong-Han,Kim, Kook-Hyung Elsevier 2009 Virology Vol.393 No.1
<P><B>Abstract</B></P><P>The complete genomes of 30 <I>Soybean mosaic virus</I> (SMV) isolates and strains were sequenced in this study. Together with fourteen previously reported sequences, we analyzed the genetic structure of the SMV population. Analyses of genetic diversity showed that different genomic regions of SMV are under different evolutionary constraints and that there was no significant genetic differentiation between East Asian and North American populations of SMV. Phylogenetic analyses revealed a significant correlation between phylogeny of the cylindrical inclusion (CI) gene of SMV and SMV resistance gene 3 (<I>Rsv3</I>)-relating pathogenicity of SMV, suggesting CI might be a pathogenic determinant in <I>Rsv3</I>-mediated disease response. Interestingly, recombination analyses identified 19 ‘clear’ recombination events in the SMV population. Furthermore, as several resistance-breaking strains were identified as recombinants, it appears that recombination might contribute to overcome host resistance in SMV–soybean pathosystem. Our finding suggests that recombination as well as mutation is an important evolutionary process in the genetic diversification of SMV population.</P>
Studies on Phytochemical Constituents from the Black Ginseng (Ginseng Radix nigra)
Changon Seo(서찬곤),Jin Kyu Kim(김진규),Seong Su Hong(홍성수),Jin Gwan Kwon(권진관),Chun Whan Choi(최춘환),Wonsik Jeong(정원식),Yun-Hyeok Choi(최윤혁),Junho Oh(오준호),Ji Hye Lee(이지혜),Joa Sub Oh(오좌섭) 한국약용작물학회 2014 한국약용작물학회 학술대회논문집 Vol.2014 No.2