http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Comparative proteomic analysis of plant responses to sound waves in Arabidopsis
권영상,Mi-Jeong Jeong,Jaeyul Cha1,Sung Woo Jeong,박수철,신성철,정우식,배한홍,Dong-Won Bae 한국식물생명공학회 2012 식물생명공학회지 Vol.39 No.4
Environmental factors greatly influence the growth,development, and even genetic characteristics of plants. The mechanisms by which sound influences plant growth,however, remain obscure. Previously, our group reported that several genes were differentially regulated by specific frequenciesof sound treatmentusing a sound-treated subtractive library. In this study, we used a proteomic approach to investigate plant responses to sound waves in Arabidopsis. The plants were exposed to 250-Hz or 500-Hz sound waves,and total proteins were extracted from leaves 8 h and 24 h after treatment. Proteins extracted from leaves were subjected to 2-DE analysis. Thirty-eight spots were found to be differentially regulated in response to sound waves and were identified using MALDI-TOF MS and MALDI-TOF/TOF MS. The functions of the identified proteins were classified into photosynthesis, stress and defense, nitrogen metabolism,and carbohydrate metabolism. To the best of our knowledge,this is the first report on the analysis of protein changes in response to sound waves in Arabidopsis leaves. These findings provide a better understanding of the molecular basis of responses to sound waves in Arabidopsis.
( Woo Jin Jun ),( Eugene Cho ),( Myung Mi Kim ),( Mi Suk Choi ),( Joong Ki Kook ),( Su Gwan Kim ),( Do Kyung Kim ),( Heung Joong Kim ),( Young Ju Cha ),( Sung Kyu Lee ),( Chun Sung Kim ) 조선대학교 구강생물학연구소 2012 Oral Biology Research (Oral Biol Res) Vol.36 No.1
Strong expression of neuron restrictive silencer factor (NRSF) has been observed in many aggressive types of cancer cells and mature neurons. However, the function of the neuron restrictive silencer element (NRSE)/NRSF system in KB human oral cancer cells is unknown. Findings from previous studies in our lab have demonstrated the importance of NRSF as a factor in regulationof cell proliferation of KB human oral cancer cells. Treatment of KB cells with NRSF siRNA resulted in signifi cant inhibitionof cell growth through repression of NRSF expression. In this study, in order to understand the NRSE/NRSF regulatory network in KB cells, we performed microarray analysis in KB cells treated with NRSF specifi c targeted siRNA. The expression profi les of several genes were further validated in KB cells treated with NRSF siRNA. Results of microarray analysis showed upregulation of 117 genes and down-regulation of 215 genes in KB cells treated with NRSF siRNA. Most of the up-regulated genes were involved in signal transduction, cell communication, cell cycle, and apoptosis;down-regulated genes were involved in RNA processing, neurogenesis, transcription factor activity, and synaptogenesis. NRSF is known as a transcriptional repressor for silencingof neuronal genes; however, according to our data, treatment of KB cells with NRSF siRNAresulted in down-regulation of more than 200 genes. As a result, genes identifi ed in this screen represent a novel control pathway via NRSF expression in KB oral cancer cells. Further investigation will be needed in order to defi ne the mechanism of gene regulation by expression of NRSF in KB human oral cancer cells.
( Woo Sung Jo ),( Ki Tae Yoon ),( Mong Cho ),( Jong Ho Hwang ),( San Kim ),( Tae Ik Park ),( Dong Hyuk Cha ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.1
Liposarcoma accounts for 5-6% of malignant tumors of the soft tissue. It is commonly seen in the limbs and retroperitoneum, but, primary liposarcoma of the liver is extremely rare. A 48-year-old women was presented to local hospital with mild headache. She was admited at neruosurgery. Coincidentally hepatic mass was founded. On physical examination, liver mass was palpable on right upper quadrant of abdomen. A CT scan showed a round tumor measuring 8cm with low density. MRI of the liver showed a high signal intensity on T1-weighted image and a high signal intensity on T2-weighted image in interlobal fissure. Liver biopsy was lipoma. During the surgery, the tumor was found to lie below liver. The mass was removed with liver wedge resection. A well circumscribed yellow mass. The resected specimen measured 85x75x70mm. Different histologic areas were separated by broad fibrous septa containing cells with enlarged hyperchromatic and atypical nuclei. Which was diagnosed as liposarcoma. In our case we thought liposarcoma was not originated in liver, glisson casule of liver was intact by pathologist, But mass was adhesive to glisson capsule of liver, which so performed segmentectomy of liver. Gallbladder was not seen in operation fields, But gallbladder was adhesived to liposarcoma on pathology and all layer of gallbladder was intact. In conclusion, our case was not originated fromliver and gallbladder. The origin was unknown. just as, liposarcoma was adhesived to liver and gallbladder.
Bendable and Splitter-Integrated Optical Subassembly Based on a Flexible Optical Board
Sung Hwan Hwang,Jung Woon Lim,Woo-Jin Lee,Gye Won Kim,Che Hyun Cho,Jong Bea An,Ki Young Jung,Kyung Soon Cha,Byung Sup Rho IEEE 2010 IEEE photonics technology letters Vol.22 No.3
<P>A bendable and splitter-integrated optical subassembly (OSA) is suggested as a short-distance board-to-board optical interconnection. This OSA was fabricated by simply packaging a vertical-cavity surface-emitting laser on a flexible optical board having an embedded 1 × 8 optical splitter waveguide. Finally, we measured various optical characteristics of the OSA, including insertion, twist, and bending losses.</P>
Woo Young Min,Seo Jae Yong,Kim Soo-ya,Cha Ji Hyun,Cho Hyun Dae,Cha Young Kwon,Jeong Ju Tae,Park Sung Min,Ryu Hwa Sun,Kim Jae Mun,Kim Moon Hoy,Kim Hee-Taek,Kim Yong-Min,Joo Kwang Sik,Lee Sun Mi,Lee Jun 한국미생물·생명공학회 2022 한국미생물·생명공학회지 Vol.50 No.4
This study investigated the hair growth effect of Schisandra chinensis extract (TS-SC) and TS-SC fermented by Lactobacillus plantarum (TS-SCLF) on human dermal papilla cells (hDPCs). The production of vascular endothelial growth factor (VEGF), insulin-like growth factor 1 (IGF-1), keratinocyte growth factor/ fibroblast growth factor 7 (KGF/FGF-7) and hepatocyte growth factor (HGF), transforming growth factor beta 1 (TGF-β1) were examined. The secretion rates of VEGF and KGF/FGF-7 were high in TS-SC, and the secretion rates of IGF-1 and HGF were high in TS-SCLF. TGF-β1 was inhibited in a concentration-dependent manner in all samples. Gene expression of VEGF, IGF-1, KGF, HGF and alkaline phosphatase, relevant to hair growth, were examined. The data revealed that TS-SC and TS-SCLF successfully promoted hair growth in hDPCs. The IGF-1 gene was expressed in a dose-dependent manner in TS-SCLF. These results indicate that TS-SC and TS-SCLF fermented extract effectively promoted hair growth and gene expression relevant to hair growth in hDPCs. Used in clinical trials the test substance ‘CMK-LPF01’ showed a statistically significant increase in the number of hairs at 8 weeks, 16 weeks, and 24 weeks compared to before product use, and a change in hair growth, a secondary efficacy evaluation variable. Through additional research in the future, it is expected that “CMK-LPF01” can be developed as a functional material that can help alleviate symptoms of hair loss.
Structural and functional insights into the B30.2/SPRY domain
Woo, Jae-Sung,Imm, Joon-Hyuk,Min, Chang-Ki,Kim, Kyung-Jin,Cha, Sun-Shin,Oh, Byung-Ha Wiley (John WileySons) 2006 The EMBO journal Vol.25 No.6
<P>The B30.2/SPRY domain is present in approximately 700 eukaryotic (approximately 150 human) proteins, including medically important proteins such as TRIM5alpha and Pyrin. Nonetheless, the functional role of this modular domain remained unclear. Here, we report the crystal structure of an SPRY-SOCS box family protein GUSTAVUS in complex with Elongins B and C, revealing a highly distorted two-layered beta-sandwich core structure of its B30.2/SPRY domain. Ensuing studies identified one end of the beta-sandwich as the surface interacting with an RNA helicase VASA with a 40 nM dissociation constant. The sequence variation in TRIM5alpha responsible for HIV-1 restriction and most of the mutations in Pyrin causing familial Mediterranean fever map on this surface, implicating the corresponding region in many B30.2/SPRY domains as the ligand-binding site. The amino acids lining the binding surface are highly variable among the B30.2/SPRY domains, suggesting that these domains are protein-interacting modules, which recognize a specific individual partner protein rather than a consensus sequence motif.</P>
Design of an 8-bit CMOS two-step current cell matrix digital-to-analog converter
Sung-Ho Yun,Pil-Soo Hyeon,Young-Sun Kim,So-Hyun Park,Hyeong-Woo Cha,Sung-Soo Kim 대한전자공학회 2007 ITC-CSCC :International Technical Conference on Ci Vol.2007 No.7
An 8-bit CMOS two-step current cell matrix digitalto-analog converter(DAC) with low-power and small size for mixed-signal system was designed. The DAC consists of a 4-bit current matrix cell, 2-step current reference, 2-step switchedcurrent(SI) memory cell, and control logic. The DAC has three operating modes of fine conversion, coarse conversion, and summing output. The simulation result shows that the DAC has good output current characteristics for digital code. The chip area was 1.0㎜ⅹ0.4㎜. Power dissipation for digital code of FF was 3.5㎽ at supply voltage 3.3V.
Shading Effect of Different Colored Polyethylene net on Seeding Growth of Panax ginseng C. A. Meyer
Sung-Woo Lee,Seon-Woo Cha,Dong-Yun Hyun,Young-Chang Kim,Seung-Won Kang,Nak-Sul Seong 韓國藥用作物學會 2006 한국약용작물학회지 Vol.14 No.2
This study was carried out to investigate spectral irradiance characteristics of blue, yellow, and blue-black colored polyethylene (PE) shading net and the effect on growth characteristics and yield in ginseng seedling. The spectral irradiance (μmol/m2/s/nm) showed the peak at 498 nm in both of blue and blue-black PE shading net, and 606 nm under yellow PE one. The intensity of blue light in blue shading was more strong than that of blue-black shading, control. Blue shading was increased by 17% and 23% in accumulated quantum for daytime, 0.5℃ and 0.2℃ in maximum temperature on June 2 than that of yellow and blue-black shading, respectively, but heat injury ratio of the former was lower than that of the latter. Chlorophyll content and stem length in blue shading were decreased more significantly than those of yellow and blue-black shading. The specific leaf weight was higher under blue and yellow shading than that of blue-black shading. Ginseng seedling harvested in blue shading was increased by 13~17% in the number of root, and 17~20% in root weight per m 2 compared to yellow and blue-black shading owing to the increase of survived plant, and the decrease of specific leaf weight, heat injury ratio, and stem length.