Follicular Variant of Papillary Carcinoma Thyroid with Massive Angioinvasion of the Internal Jugular Vein: Our Approach

Roshan Kumar Verma,Deepak Sarahan,Gautamjeet Raj Kumar 대한갑상선학회 2020 International Journal of Thyroidology Vol.13 No.2

Follicular variant of papillary carcinoma thyroid is an aggressive variant of papillary carcinoma thyroid. It is morecommonly associated with extrathyroidal extension and regional lymphadenopathy. It can rarely be associated withmicroscopic vascular invasion but tumor thrombus into great veins is a rare phenomenon. We present a caseof 60-year-old male with follicular variant of papillary carcinoma thyroid with tumor thrombosis in superior thyroidvein and internal jugular vein (IJV). We report a case of a 60-year-old male who presented with a large swellingin the lower part of left side of neck for 4 months. Clinical examination revealed a hard swelling of 12x8 cmin left side of neck. Ultrasonography showed a solitary thyroid mass of the left lobe and a dilated left superiorthyroid vein and ipsilateral IJV. Fine-needle aspiration cytology revealed follicular variant of PTC cells. Totalthyroidectomy was done. A tumor thrombus was discovered in the superior thyroid vein and left IJV was foundto be dilated. The left IJV with superior thyroid vein was ligated and excised. The patient recovered well afterthe operation with no local or distant metastasis detected. Follicular variant of PTC commonly spreads to the lymphnodes. Vascular spread via direct intravascular extension through superior thyroid vein is extremely rare. Onpalpation cord like IJV is felt on the involved side. Neck ultrasound play important role in the diagnosis. Aggressivesurgical treatment with IJV ligation above and below the tumor thrombus is recommended to minimize the riskof potentially fatal complications of the intraluminal masses. Intravascular tumor extension into IJV of neck infollicular variant of PTC is rare and can be associated with serious consequences. Total thyroidectomy withthrombectomy with ligation of IJV must be done.

Docetaxel-Loaded Polylactic Acid-Co-Glycolic Acid Nanoparicles: Formulatuin, Physicochemical Characterization and Cytotoxicity Studies

( Roshan Pradhan ),( Bijay Kumar Poudel ),( Thiruganesh Ramasamy ),( Han Gon Choi ),( Chul Soon Yong ),( Jong Oh Kim ) 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0

In the present study, we developed novel docetaxel (DTX)-loaded polylactic acid-co-glycolic acid(PLGA) nanoparticles (NPs) using the combination of sodium lauryl sulfate (SLS) and poloxamer 407, the anionic and non-ionic surfactants respectively for stabilization. The NPs were prepared by emulsification/solvent evaporation method. The combination of these surfactants at weight ratio of 1:0.5 was able to produce uniformly distributed small sized NPs and demonstrated the better stability of NP dispersion with high encapsulation efficiency (85.9 +/- 0.6%). The drug/polymer ratio and phase ratio were 2:10 and 1:10, respectively. The optimized formulation of DTX-loaded PLGA NPs had a particle size and polydispersity index of 104.2 +/- 1.5 nm and 0.152 +/- 0.006, respectively, which was further supported by TEM image. In vitro release study was carried out with dialysis membrane and showed 32% drug release in 192 h. When in vitro release data were fitted to Korsmeyer-Peppas model, the n value was 0.481, which suggested the drug was released by anomalous or non-Fickian diffusion. In addition, DTX-loaded PLGA NPs in 72 h, displayed approximately 75% cell viability reduction at 10 microg/ml DTX concentration, in MCF-7 cell lines, indicating sustained release from NPs. Therefore, our results demonstrated that incorporation of DTX into PLGA NPs could provide a novel effective nanocarrier for the treatment of cancer.

Preparation and evaluation of 17-allyamino- 17-demethoxygeldanamycin (17-AAG)-loaded poly(lactic acid-co-glycolic acid) nanoparticles

Roshan Pradhan,Bijay Kumar Poudel,최주연,최임순,신범수,최한곤,용철순,김종오 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.5

In the present study, we developed the novel17-allyamino-17-demethoxygeldanamycin (17-AAG)-loadedpoly(lactic acid-co-glycolic acid) (PLGA) nanoparticles(NPs) using the combination of sodium lauryl sulfateand poloxamer 407 as the anionic and non-ionic surfactantfor stabilization. The PLGA NPs were prepared by emulsification/solvent evaporation method. Both the drug/polymer ratio and phase ratio were 1:10 (w/w). The optimizedformulation of 17-AAG-loaded PLGA NPs had aparticle size and polydispersity index of 151.6 ± 2.0 and0.152 ± 0.010 nm, respectively, which was further supportedby TEM image. The encapsulation efficiency anddrug loading capacity were 69.9 and 7.0 %, respectively. In vitro release study showed sustained release. Whenin vitro release data were fitted to Korsmeyer–Peppasmodel, the n value was 0.468, which suggested that thedrug was released by anomalous or non-Fickian diffusion. In addition, 17-AAG-loaded PLGA NPs in 72 h, displayedapproximately 60 % cell viability reduction at 10 lg/ml17-AAG concentration, in MCF-7 cell lines, indicatingsustained release from NPs. Therefore, our resultsdemonstrated that incorporation of 17-AAG into PLGANPs could provide a novel effective nanocarrier for thetreatment of cancer.

Docetaxel-loaded polylactic acid-co-glycolic acid nanoparticles: formulation, physicochemical characterization and cytotoxicity studies.

Pradhan, Roshan,Poudel, Bijay Kumar,Ramasamy, Thiruganesh,Choi, Han-Gon,Yong, Chul Soon,Kim, Jong Oh American Scientific Publishers 2013 Journal of Nanoscience and Nanotechnology Vol.13 No.8

<P>In the present study, we developed novel docetaxel (DTX)-loaded polylactic acid-co-glycolic acid (PLGA) nanoparticles (NPs) using the combination of sodium lauryl sulfate (SLS) and poloxamer 407, the anionic and non-ionic surfactants respectively for stabilization. The NPs were prepared by emulsification/solvent evaporation method. The combination of these surfactants at weight ratio of 1:0.5 was able to produce uniformly distributed small sized NPs and demonstrated the better stability of NP dispersion with high encapsulation efficiency (85.9 +/- 0.6%). The drug/polymer ratio and phase ratio were 2:10 and 1:10, respectively. The optimized formulation of DTX-loaded PLGA NPs had a particle size and polydispersity index of 104.2 +/- 1.5 nm and 0.152 +/- 0.006, respectively, which was further supported by TEM image. In vitro release study was carried out with dialysis membrane and showed 32% drug release in 192 h. When in vitro release data were fitted to Korsmeyer-Peppas model, the n value was 0.481, which suggested the drug was released by anomalous or non-Fickian diffusion. In addition, DTX-loaded PLGA NPs in 72 h, displayed approximately 75% cell viability reduction at 10 microg/ml DTX concentration, in MCF-7 cell lines, indicating sustained release from NPs. Therefore, our results demonstrated that incorporation of DTX into PLGA NPs could provide a novel effective nanocarrier for the treatment of cancer.</P>

Preparation and characterization of alginate gel core-lipid nanocapsules for co-delivery of hydrophilic and hydrophobic anti-cancer drugs

Bijay Kumar Poudel,김종오,용철순,Roshan Pradhan,Biki Gupta,최주연 한국약제학회 2014 Journal of Pharmaceutical Investigation Vol.44 No.7

Novel alginate gel-core lipid nanocapsules(LNCs) consisting of hydrogel islets in an oil filled matrixand surrounded by a polymeric shell were prepared by hothomogenization and double emulsion method, for codeliveryof both hydrophilic (gemcitabine) and hydrophobic(paclitaxel) anticancer drugs. This system was preparedusing a relatively simple and organic solvent-free methodusing only excipients that are approved by the FDA. Theliquid oil matrix allowed the solubilization and encapsulationof hydrophobic drugs such as paclitaxel, whereasalginate hydrogel islets encapsulated hydrophilic drugssuch as gemcitabine. Alginate gel-core LNCs were characterizedwith respect to particle size, polydispersity index(PDI), morphology, encapsulation efficiency, and in vitrorelease. Dual drug-loaded alginate gel-core LNCs had aparticle size of 171.8 ± 4.1 nm and PDI of 0.201 ± 0.005,respectively, and showed core–shell type spherical morphologywith a smooth surface. In addition, alginate gelcore-LNCs underwent sustained release of both drugs. These results suggest that alginate gel core-LNCs may beused for co-delivery of hydrophilic (gemcitabine) andhydrophobic (paclitaxel) anticancer drugs.

전기방사와 기상중합법을 이용한 전도성 콜라겐 나노섬유 제작

김지희,Roshan Khadka,Samayanan Selvam,Praveen Kumar,최종섭,임진형 한국고분자학회 2023 한국고분자학회 학술대회 연구논문 초록집 Vol.48 No.2

Manuka Honey versus Antibiotic Ear Drops in Healing of Post-Operative Mastoid Cavity: A Prospective Randomized Trial

Niveditha Damodharan,Roshan Kumar Verma,Archana Angrup,Naresh K Panda,Naresh K Panda 대한이비인후과학회 2020 대한이비인후과학회지 두경부외과학 Vol.63 No.5

Background and Objectives This study investigates the role of manuka honey in the healingof postoperative mastoid cavity. Subjects and Method This was a single centre prospective study on 40 consecutive patientsof chronic otitis media undergoing canal wall down mastoidectomy. Manuka honey soaked ingel foam was kept in the mastoid cavity for the study group and antibiotic soaked gel foam waskept for the control group. Culture swabs from mastoid granulations were sent at various timesfrom both groups. The healing of the mastoid cavity was assessed in the follow up period. Results Preoperatively 15 out of 20 patients (75%) had a positive aural swab culture in thestudy group while 11 out of 20 (55%) in the control group had a positive aural swab culture. Themost common organism isolated was Pseudomonas aeruginosa and Proteus mirabilis. Onemonth after mastoidectomy only 4 patients (20%) had sterile culture and 16 patients (80%) hadgrown organisms; in the control group, 7 patients (35%) had sterile culture and 13 patients (65%)had growth on culture. The mean merchant scores for the study group and the control were 2.61(2-5) and 2.05 (1-4), respectively. At 3 months 13 patients (65%) with sterile culture and 7 patients(35%) had growth on culture; in the control group, 16 patients (80%) had sterile cultureand 4 had shown persistent growth on culture (p=0.28). All positive cultures were aerobic inboth groups. The mean merchant scores for the study group and the control were 1.03 (0-4)and 0.7 (0-3), respectively (p=0.09). Conclusion Healing of mastoid cavity was almost similar in both groups (p>0.05). Manukahoney exhibited antibacterial activity against Pseudomonas, Proteus, Klebsiella, Escherichiacoli, Staphylococcus

A novel surface-attached carvedilol solid dispersion with enhanced solubility and dissolution

Sung Neung Lee,Bijay Kumar Poudel,Tuan Hiep Tran,Nirmal Marasini,Roshan Pradhan,Young Im Lee,Dong Won Lee,Jong Soo WOO,최한곤,용철순,김종오 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.1

A novel surface-attached, spray-dried soliddispersion containing poorly water-soluble carvedilol (CV)without any change in the crystallinity was prepared usingwater, polyvinylpyrrolidone (PVP K30) and Tween 80. Thesolid dispersion was optimized by investigating the effectsof the weight ratios of Tween 80/PVP K30 and carrier/drugon the aqueous solubility of CV. The optimum solid dispersionconsisted of a relatively low carrier to drug weightratio: the weight ratio of CV/PVP K30/Tween 80 was 12/4/2. Unlike conventional methods of solid dispersion preparation,this method yielded CV-loaded solid dispersion withno change in the crystallinity of the drug as was evidentfrom SEM, DSC and XRD. It was demonstrated that thesolid dispersions prepared had hydrophilic carriers attachedto the surface of the drug, thus changing it from a hydrophobicto a hydrophilic form without changing the crystallineform. The optimized solid dispersion improved thedrug solubility and dissolution rate by about 11,500-foldand twofold, respectively. It was further suggested that thismethod of solid dispersion preparation is better than conventionalmethods in terms of environmental and industrialstandpoints. Thus, it was concluded that CV-loaded soliddispersion prepared using this method would be of use fordelivering poorly water-soluble CV with enhanced solubilityand dissolution, but without crystalline changes.

Developmental stage-dependent differential gene expression of superoxide dismutase isoenzymes and their localization and physical interaction network in rice (Oryza sativa L.)

Krishna Nath,이춘환,Susheel Kumar,Roshan Sharma Poudyal,양영남,Rupak Timilsina,박유신,Jayamati Nath,Puneet Singh Chauhan,Bijaya Pant 한국유전학회 2014 Genes & Genomics Vol.36 No.1

Superoxide dismutase (SOD) isoenzymes areessential for scavenging excess reactive oxygen species inliving organisms. So far, expression pattern of SOD isoenzymesgenes along leaf development plus their sub-cellularlocalization and physical interaction network have notyet been clearly elucidated. Using multiple bioinformaticstools, we predicted the sub-cellular localizations of SODisoforms and described their physical interactions in rice. Using in silico approaches, we obtained several evidencesfor existence of seven SOD genes and a SOD copperchaperone gene. Their transcripts were differentiallyexpressed along with different developmental stage of riceleaf. Finally, we performed quantitative real time-polymerasechain reaction (qRT-PCR) to validate in silico differential expression pattern of SOD genes experimentally. Expression of two cytosolic cCuZn-SODs was highduring the whole vegetative stage. Two plastidic Fe-SODswere found and their expression levels were very low andstarted to increase from the late vegetative stage. Theirexpression patterns were very similar to each other, indicatingthe formation of heterodimer. However, theirexpression patterns are different from those for ArabidopsisFe-SODs. The expression of pCuZn-SOD was very high inthe early developmental stage, but qRT-PCR results weredifferent, which remains for further study. From the resultson the differential expression of SOD genes, we canunderstand the role of each SOD gene and even predicttheir role under certain circumstances based on in silicoanalysis.

RESEARCH ARTICLE : A novel surface-attached carvedilol solid dispersion with enhangced solubility and dissolution

( Sung Neung Lee ),( Beijay Kumar Poudel ),( Tuan Hiep Tran ),( Nirmal Marasini ),( Roshan Pradhan ),( Young Im Lee ),( Dong Won Lee ),( Jong Soo Woo ),( Han Gon Choi ),( Chul Soon Yong ),( Jong Oh Ki 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0

A novel surface-attached, spray-dried solid dispersion containing poorly water-soluble carvedilol (CV) without any change in the crystallinity was prepared using water, polyvinylpyrrolidone (PVP K30) and Tween 80. The solid dispersion was optimized by investigating the effects of the weight ratios of Tween 80/PVP K30 and carrier/drug on the aqueous solubility of CV. The optimum solid dispersion consisted of a relatively low carrier to drug weight ratio: the weight ratio of CV/PVP K30/Tween 80 was 12/4/2. Unlike conventional methods of solid dispersion preparation, this method yielded CV-loaded solid dispersion with no change in the crystallinity of the drug as was evident from SEM, DSC and XRD. It was demonstrated that the solid dispersions prepared had hydrophilic carriers attached to the surface of the drug, thus changing it from a hydrophobic to a hydrophilic form without changing the crystalline form. The optimized solid dispersion improved the drug solubility and dissolution rate by about 11,500-fold and twofold, respectively. It was further suggested that this method of solid dispersion preparation is better than conventional methods in terms of environmental and industrial standpoints. Thus, it was concluded that CV-loaded solid dispersion prepared using this method would be of use for delivering poorly water-soluble CV with enhanced solubility and dissolution, but without crystalline changes.