Elevated TRAF2/6 expression in Parkinson's disease is caused by the loss of Parkin E3 ligase activity.

Chung, Ji-Yun,Park, Hee Ra,Lee, Su-Jin,Lee, Sun-Hye,Kim, Jin Sik,Jung, Youn-Sang,Hwang, Sang Hyun,Ha, Nam-Chul,Seol, Won-Gi,Lee, Jaewon,Park, Bum-Joon United States and Canadian Academy of Pathology [e 2013 Laboratory investigation Vol.93 No.6

<P>Parkinson's disease (PD) is the second leading neurodegenerative disease, and is known to be induced by environmental factors or genetic mutations. Among the verified genetic mutations of PD, Parkin, isolated from the PARK2 locus, shows an autosomal recessive inheritance pattern and is known to be an E3 ligase. However, the physiological target of Parkin and the molecular mechanism of Parkin-deficiency-induced PD have not been clearly demonstrated until now. It has recently been proposed that inflammation, suggesting as a causal factor for PD, is enhanced by Parkin deficiency. Thus, we examined the relationship between inflammation-related factors and Parkin. Here, we provide the evidence that Parkin suppresses inflammation and cytokine-induced cell death by promoting the proteasomal degradation of TRAF2/6 (TNF-α receptor-associated factor 2/6). Overexpression of Parkin can reduce the half-lives of TRAF2 and TRAF6, whereas si-Parkin can extend them. However, mutant Parkins did not alter the expression of TRAF2/6. Thus, loss of Parkin enhances sensitivity to TNF-α- or IL-1β-induced JNK activation and NF-κB activation. Indeed, si-Parkin-induced apoptosis is suppressed by the knockdown of TRAF6 or TRAF2. We also observed elevated expression levels of TRAF6 and a reduction of IκB in an 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mouse model. Moreover, elevated expression levels or aggregation of TRAF6 were detected in approximately half of the human PD tissues (7/15 cases) and 2 cases, respectively. In addition, TRAF6 and Parkin expression levels show a reverse relationship in human PD tissues. Our results strongly suggest that the reduction of Parkin or overexpression of TRAF2/6 by chronic inflammation would be the reason for occurrence of PD.</P>

Growth Inhibition of Colon Cancer through Inactivation of STAT3 Pathway by IL-10 and IL-1ra Released from Murine Macrophage

Jin Tae Hong, Dohee Won1, Mi Hee Park, Sun Mi Kown, Miran Jo, Sang-Yoon Nam, Beom Jun Lee, Young Won Yun, Ki-Wan Oh, Sang Bae Han 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.4

The objective of this study was to determine the effect of macrophages on growth of human colon cancer cells. The results showed that co-culture of colon cancer cells with macrophages inhibited the growth of colon cancer cells (HCT116 and SW620) depending on the number of macrophages, RAW 264.7 cells, and activated THP-1 cells accompanied by down regulation of pSTAT3 in cancer cells. We also found that expression and release of cancer cell growth inhibitory cytokines, IL-1 receptor antagonist (IL-1ra) and IL-10, was increased in macrophages. Blocking of the STAT3 pathway with specific inhibitor and siRNA of STAT3 abolished the growth of colon cancer cells and expression of IL-1ra and IL-10. In addition, neutralization of IL-1ra and IL-10 with antibodies resulted in reversal of macrophage-induced inhibition of cancer cell growth. These data showed that IL-1ra and IL-10 released from macrophages inhibit growth of colon cancer cells through inhibition of the STAT3 pathway

국립공원 아고산대 독립훼손지 복원식생 분포특성 및 관리방안

김종엽(Jong-Yup Kim),윤주웅(Ju-Ung Yun),한봉호(Bong-Ho Han),곽정인(Jeong-In Kwak),박석철(Seok-Cheol Park),박수영(Su Young Park),정원옥(Won-Ok Jeong) 국립공원연구원 2022 국립공원연구지 Vol.13 No.1

본 연구는 독립훼손지 중 아고산대에 위치한 지리산국립공원 세석평전과 노고단 복원식생지를 대상으로 현존식생 분석 및 식생분포 특성을 파악하여 중장기적인 모니터링 및 관리방안 수립을 위한 기초자료를 제공하고자 수행하였다. 현존식생 조사범위는 훼손지 일대 유역권분포 현황을 고려하여 설정하였으며, 세석평전 조사면적은 55,037㎡, 노고단 조사면적은 166,599㎡ 이었다. 세석평전은 초본층에 의한 식생피복도는 90% 이상 수준으로서 나지노출이 없었고, 관목층의 피복도가 60% 이상으로 안정화되어 있었다. 주요 출현수종은 구상나무, 사스래나무, 쇠물푸레나무, 철쭉, 털진달래, 붉은병꽃나무 등 식재수종과 자연적으로 이입한 자생수종이 생육하고 있었다. 노고단은 신갈나무, 호랑버들, 철쭉, 털진달래, 미역줄나무 등이 우점하는 관목식생지가 52%로 가장 넓게 분포하고 있었고, 전반적으로 식생활착 상태가 양호하였다. 노고단의 원식생은 신갈나무군락으로 추정되었고, 군부대철거지에는 귀화식물이 분포하고 있기 때문에 적극적인 관리가 필요하였다. 연구대상지는 장기적으로 주변 자연식생과 조화로운 식생경관으로 발달할 것으로 예측되었다. 향후 훼손지 복원모델은 인근 지역의 원식생을 고려하여 설정해야 할 것이지만, 복원 달성 목표는 실현가능한 수준의 목표를 설정해야 할 것이다. This study was conducted to provide basic data for mid-to long-term monitoring and establishment of management plans by analyzing the existing vegetation and identifying the vegetation distribution characteristics for the restored vegetation in Nogodan and Seseokpyeongjeon in Jirisan National Park located in the subalpine zone among the independent damaged areas. Actual vegetation survey range was established in consideration of the distribution of the watershed around the damaged area. The surveyed area of Seseokpyeongjeon was 55,037㎡, and the surveyed area of Nogodan was 166,599㎡. In Seseokpyeongjeon, the vegetation coverage by the herbaceous layer was almost 90% or more, there was no bare exposure, and the coverage of the shrub layer was stabilized at 60% or more. The main emerging tree species were planted species such as Abies koreana, Betula ermanii, Fraxinus sieboldiana, Rhododendron schlippenbachii, Rhododendron mucronulatum var. ciliatum, and Weigela florida as well as natively migrated native species. In Nogodan, the shrub vegetation dominated by Quercus mongolica, Salix caprea, Rhododendron schlippenbachii, Rhododendron mucronulatum var. ciliatum, and Tripterygium regelii was the most widely distributed with 52% of the area, and the overall diet was good. The native vegetation of Nogodan was presumed to be a cypress colony. Naturalized plants were distributed in the military base demolition site, so active management was required. The study site was predicted to develop into a vegetation landscape in harmony with the surrounding natural vegetation in the long term. In the future, the restoration model for damaged areas should be set in consideration of the native vegetation of the nearby area, but the goal to achieve restoration should be set at a feasible level.

사상체질에 따른 신체화 기제의 차이에 대한 연구

이고은 ( Go Eun Lee ),박장호 ( Jang Ho Park ),유윤선 ( Yun Sun Lyu ),노동진 ( Dong Jin No ),박인숙 ( In Sook Park ),강형원 ( Hyung Won Kang ) 대한한방신경정신과학회 2011 동의신경정신과학회지 Vol.22 No.3

Objectives: The purpose of this study was to investigate the difference of somatization psychological mechanism among Sasang constitution. Methods: Subjects were seventy four students of Won-Kwang university responded the questionnaire. We evaulated teh subjects using Minnesota Questionnaire for the Sasang Constitution Classification Ⅱ(QSCC Ⅱ), Multiphasic Personality Inventory-2(MMPI-2), 20-item Toronto Alexithymia Scale (TAS-20), Somato-Sensory Amplification Scale(SSAS), Symptom Interpretation Questionnaire(SIQ) and The Ways of Stress Coping Questionnaire(SCQ). Results: From the results of Clinical scale of MMPI-2, the scores of 2nd, 9th, 10th scales was significantly difference. In Lesser Yin group 10th score was the significantly highest of the others, 1st, 3rd, 6th, 7th, 8th score were the highest but not significant. and From the results of PSY-5 scale(The Personality Psychopathology Five scale) of MMPI-2, In Lesser Yin goup, INTR(Introversion/Low Positive Emotion) score was significantly highest, PSYC(psychoticism), NEGE (Negative Emotionality/Neuroticism) was higher than the others. In TAS-20K, SSAS, SIQ and SCQ Lesser Yin group was not significantly difference. but Lesser Yin group had the tendency of difficulty describing feelings and externally oriented thinking and higher score in physical and psychological interpretation. We observed generally lower levels of stress coping strategy scores than the others. Conclusions: These results showed that Lesser Yin group tend to somatization compared other constitution.

Notes on bird species newly recorded in Korea

Kim, Hwa-Jung,Kim, Dong-Won,Hur, Wee-Haeng,Jang, Byoungsoon,Lee, Yun-Kyung,Kwon, In-Ki,Park, Jong-Hyun,Park, Jong-Gil,Kang, Chang-Wan,Kim, Eun-Mi,Choi, Soon-Kyoo,Kim, Han-Kyu The National Institute of Biological Resources 2020 Journal of species research Vol.9 No.4

A total of 527 species of birds have been recorded in Korea. We summarize the records of three genera and six species that have been newly reported in Korea, discovered through the avian monitoring programs carried out by The National Institute of Biological Resources from 2013 to 2016. These six newly recorded species are; Ring-necked Duck (Aythya collaris), Lesser Scaup (Aythya affinis), Bufflehead (Bucephala albeola), Black-winged Kite (Elanus caeruleus), Mongolian Lark (Melanocorypha mongolica) and Crested Myna (Acridotheres cristatellus). One Ring-necked Duck was observed on March 23, 2014 in Jungrangcheon Stream. One male Lesser Scaup was recorded on February 24, 2014 in Gyeongpoho Lagoon. A male bufflehead was first seen in Yeongrangho Lagoon on January 26, 2013. The Black-winged Kite was first observed on January 23, 2013 at the Gangseo Wetland Ecological Park. One Mongolian Lark was also recorded on May 11, 2016 and May 12, 2017 in Marado Island. The Crested Myna was first observed on April 20, 2016 in Seomando, Island.

오공약침의 안전성에 대한 임상적 연구

소기숙,최회강,박소영,고강훈,김성남,이옥자,윤민영,문형철,김성철,이정훈,나원경,So, Ki-suk,Choi, Hoi-kang,Park, So-young,Koh, Kang-hun,Kim, Sung-nam,Lee, Ok-ja,Yun, Min-young,Mun, Hyung-chul,Kim, Sung-chul,Lee, Jung-hun,Na, Won-kyung 대한침구의학회 2004 대한침구의학회지 Vol.21 No.1

Objective: Recently scolopendrid aquacupuncture has been a good effect on pain control but it has not been known about clinical safety. So, In order to prove the clinical safety of scolopendrid aquacupuncture, We have observed the physical reac-tion and clinical pathology test after scolopendrid aquacupuncture treatment. Methods: We analyzed physical reaction and clinical pathology test before and after Scolopendrid aquacupuncture treatment of 30 patients suffering from pain, who admitted department of Acupunture and Moxibustion, College of Oriental Medicine, Won-Kwang University Kwangju hospital. Results & Conclusions: The results were summarized as follows. 1) The distribution of sex was 14 males and 16 females, and the average of patients age was 46.2 years. 2) The distribution of symptom was lumbago, lumbago with radiating pain, nuchal pain and knee joint pain. 3) In the 30 patients treated with Scolopendrid aquacupuncture, hematologic test did not show remarkable change. 4) In the 30 patients treated with Scolopendrid aquacupuncture, Liver function test(AST, ALT, ALP) showed a slight decrease on the contrary, and abnormal rate showed a decrease of 1.0%(from 3.3% to 2.3%) compared with previous study. 5) In the 30 patients treated with Scolopendrid aquacupuncture, Renal function test(BUN, Cr) and abnormal rate(from 2.5% to 2.0%) showed a slight decrease on the contrary. 6) In the 30 patients treated with Scolopendrid aquacupuncture. Electrolyte were normal range before & after treatment. 7) In the results of the Urine analysis of 30 patients, Leukocyte, Protein. Glucose, Keton, Bilirubin, U-bilinogen were not detected before and after Scolopendrid aquacupuncture treatment, and the rest almost made no difference. 8) In the Physical reactions, all of the patients complained of pain of body partially, only one patient showed reddish and itch, but symptoms like those were entirely disappeared within 24 hours and whole body pain, swelling, headache, dizziness, fatigue and nausea was not observed.

자동착유시스템 설치농가의 설치 후 만족도에 관한 실태조사

기광석,김종형,정영훈,김윤호,박성재,김상범,이왕식,이현준,조원모,백광수,김현섭,권응기,김완영,여준모,Ki, Kwang-Seok,Kim, Jong-Hyeong,Jeong, Young-Hun,Kim, Yun-Ho,Park, Sung-Jai,Kim, Sang-Bum,Lee, Wang-Shik,Lee, Hyun-June,Cho, Won-Mo,Baek, Kwang- 한국축산환경학회 2011 축산시설환경학회지 Vol.17 No.1

본 시험은 국내에 도입되기 시작한 AMS에 대하여 설치 동기, 설치 후 우유생산량과우유품질, AMS 설치계기 및 설치 후 만족도, AMS 운영상 애로점 및 건의사항 등을 조사 분석하였다. 설치 계기가 무엇인가에 대한 응답비율은 노동력 부족 44%, 체험목장으로 전환 25%, 소들의 고능력화에 대응 19% 순이었으며 기타 6%였다. AMS 설치농가의 사육경력은 평균 24.9년, 평균 연령은 53.2세로 나타났으며 가족노동력만으로 경영하는 목장이 조사농가의 38%, 고용+자가노동력 형태의 목장이 63%로 나타났다. 목장의 산유능력은 두당 평균 유량은 1일 $30.9{\ell}$, 평균 유지율 3.9%, 세균수는 9.1천개/$m{\ell}$ 수준이었다. 후계자 육성에 대한 질문에 매우 그렇다라고 응답한 비율은 68%였으며, 보통 18%, 아니다와 매우 아니다라고 응답한 비율은 18%로 나타났다. 시스템 운영현황 및 애로사항을 파악하기 위하여 AMS의 프로그램 주운영자에 대한 질문에 본인 44%, 부인 6%, 자녀 또는 후계자사람이 44%, 기타 6%나 되었다. AMS 운영상 가장 어려운 점에 대한 조사결과, 기기 작동 및 복잡한 프로그램 운영이 각각 31%로 가장 많았다. 그 외 기계 에러 및 고장에 대한 애로사항이 25%이였다. 도태원인에 대하여 유방염 28%, 번식장애 20%, 발굽질환 19%, AMS에 부적합한 유두 배열 12%, 분만 후 대사성질병 7%, 노산 등 기타 14%였다. 시스템 설치 후 유지보수 계약 체결유무 및 비용에 대하여 유지보수 계약 56%, 유지 보수 계약 미체결 44%이었다. 유지보수 계약 평균 계약비용은 연간 658만원 정도였다. 시스템 설치 후 항목별 만족도에 대하여 5점 만점에 3.9점이었으며, 노동력 절감 3.7점, 업체의 A/S 3.6점, 산유량 증가 3.2점, 원유의 체세포 수 저감 2.8점이었다. 시스템 설치 시 고려사항 및 건의사항에 대하여 고가의 장비를 효율적으로 사용하기 위하여 젖소의 유방과 유두 배열이 적합하도록 젖소를 선발하는 것, 시스템을 운용할 수 있는 능력, 적합한 환경, 재정적 능력과 부지 확보, 지속적인 관찰 등이 권장되어야 할 것으로 나타났으며, 설치 업체에 대해서는 컨설팅이 체계화될 필요가 가장 높게 나타났으며 이어서 보다 저렴한 설치와 서비스의 체계화를 요구하는 결과를 보였다. The present survey was conducted to provide basic information on automatic milking system (AMS) in relation to purchase motive, milk yield and quality, customer satisfaction, difficulties of operation and customer suggestions, etc. Purchase motives of AMS were insufficient labor (44%), planning of dairy experience farm (25%), better performance of high yield cows (19%) and others (6%), respectively. Average cow performance after using AMS was 30.9l/d for milk yield, 3.9% for milk fat, 9,100/ml for bacterial counts. Sixty-eight percentage of respondents were very positive in response to AMS use for their successors but 18% were negative. The AMS operators were owner (44%), successor (44%), wife (6%) and company worker (6%), respectively. The most difficulty (31%) in using AMS was operating the system and complicated program manual. The rate of response to system error and breakdown was 25%. The reasons for culling cow after using AMS were mastitis (28%), reproduction failure (19%), incorrect teat placement (12%), metabolic disease (7%) and others (14%), respectively. Fifty-six percentages of the respondents made AMS maintenance contract and 44% did not. Average annual cost of the maintenance contract was 6,580,000 won. Average score for AMS satisfaction measurement (1 to 5 range) was 3.2 with decrease of labor cost 3.7, company A/S 3.6, increase of milk yield 3.2 and decrease of somatic cell count 2.8, respectively. Suggestions for the higher efficiency in using AMS were selecting cows with correct udder shape and teat placement, proper environment, capital and land, and attitude for continuous observation. Systematic consulting was highly required for AMS companies followed by low cost for AMS setup and systematization of A/S.

국립공원 탐방로 안전지수 및 등급분석에 관한 연구

박준효(Pak, Jun-hou),전계원(Jun, Kye-Won),박상준(Park, Sang-Jun),김명준(Kim, Myeong-Jun),윤주웅(Yun, Ju-Ung) 한국산림휴양복지학회 2020 한국산림휴양학회지 Vol.24 No.4

본 연구에서는 탐방로 안전성 평가 모델을 활용하여 21개 국립공원을 대상으로 안전지수와 등급분석을 수행하였다. 현장조사를 통해 탐방로 주변 위험요소 중 하나라도 확인되면 취약구간으로 판단하여 안전지수를 산출하였다. 취약구간은 총 180.4km로 조사되었으며 탐방로 총 연장 1,995.5km 대비 약 9.0%로 확인되었다. 안전지수는 총 200점 중 최소 42.3점에서 최대 167.0점 분포를 보이고 평균값은 101.5점으로 나타났다. 안전지수를 토대로 등급을 분석한 결과, ‘관심(Concern)’ 등급은 60.8km(34%), ‘주의(Caution)’ 등급은 57.8km(32%), ‘위험(Dangerous)’ 등급은 49.6km(27%), ‘매우위험(Very dangerous)’ 등급은 12.2km(7%)로 나타났다. 매우위험 구간은 북한산국립공원(6.0km), 지리산국립공원(3.3km), 오대산국립공원(1.6km), 설악산국립공원(0.9km), 월악산국립공원(0.4km), 속리산국립공원(0.01km) 등 6개 국립공원 탐방로에서 총 12.2km로 나타났다. 북한산국립공원은 Y계곡, 의상능선 등이 해당되며, 지리산국립공원은 칠선계곡, 구룡계곡에서 확인되었다. 오대산국립공원은 노인봉 일원, 설악산국립공원은 공룡능선, 흘림골 등에서 나타났다. 월악산국립공원은 금수산 일원, 속리산국립공원 막장봉 구간이 매우위험 구간으로 나타났다. 본 연구는 향후 재난위험 저감대책 수립과 탐방로 정비 우선순위 선정 등 국립공원 안전관리 정책을 수립하기 위한 기초자료로 활용될 수 있다. In this study, the safety indexing and grading analysis were conducted for 21 national parks, using the trail safety evaluation model. If any one of the risk factors around the trail was identified through a field survey, the safety index was calculated by judging it as a vulnerability zone. The vulnerability zone was 180.4km in total, and it was found to be about 9.0% compared to the total length of 1,995.5km. The safety index was distributed from the minimum of 42.3 to the maximum of 167.0, and the average value was 101.5 of total 200 scores. As a result of analyzing the grade based on the safety index, 60.8km (34%) was in the ‘Concern’ grade, 57.8km (32%) was in the ‘Caution’ grade, 49.6km (27%) was in the ‘Dangerous’ grade, and 12.2km (7%) was in the ‘Very dangerous’ grade. ‘Very dangerous’ sections were found to be a total of 12.2 km. including 6 national park trails: Bukhansan National Park (6.0km), Jirisan National Park (3.3km), Odaesan National Park (1.6km), Seoraksan National Park (0.9km), Woraksan National Park (0.4km), Songnisan National Park (0.01km). In terms of ‘Very dangerous’ section, Bukhansan National Park included Y Valley and Uisang Ridge, and Jirisan National Park included Chilseon Valley and Guryong Valley. The area of Noinbong in Odaesan National Park, and Gongnyong Ridge and Heulimgol in Seoraksan National Park were included. And the Geumsusan area in Wolaksan National Park and Makjangbong in Songnisan National Park were found to be ‘Very dangerous’. This study could be used as basic data to establish national park safety management policies, such as selecting priority of trail maintenance and establishing countermeasures to reduce disaster risk.

Comparative Analysis of Risk Factors on Korea National Park Trails - Focusing on Bukhansan and Seoraksan National Park -

Jun Hyo Park,Ju Ung Yun,Jong Sun Kim,Kye Won Jun 위기관리 이론과 실천 2022 Crisisonomy Vol.18 No.3

국립공원은 대부분 산지에 위치하는 지리적 특성상 자연재해 위험요인들이 산재되어 있고, 각종 안전사고로 인한 인명 및 재산피해가 지속적으로 발생되고 있다. 본 연구는 선행연구를 통해 수집된 탐방로 안전성 평가 자료를 기반으로 유사한 특성을 보이는 북한산국립공원과 설악산국립공원의 위험요인 분포 및 특성을 비교 분석하여 효율적인 탐방로 안전관리 방안을 제시하고자 하였다. 북한 산국립공원은 추락, 미끄러짐 등 안전사고로 인한 위험요인이 매우 높게 분포하는 반면, 설악산국립 공원은 안전사고보다 낙석 위험요인의 분포가 높은 것으로 나타났다. 이러한 결과는 기존의 탐방로 안전관리의 획일적 정책방향을 개선하고 유사환경의 국립공원이라도 지질, 지형, 탐방객 특성 등 환경적 요인에 따라 세분화된 기준 수립의 필요성을 도출하였다. 본 연구는 향후 탐방로 안전관리 기술 고도화와 전문성 향상, 체계적인 안전관리로 인한 인명 및 재산피해 예방에 기여할 것으로 기대된다. National parks are geographically located in mountain areas, and natural disaster of risk factors are scattered, and damage to human life and property is continuously occurring due to various safety accidents. This study tried to suggest an efficient trail safety management plan by comparing and analyzing the distribution and characteristics of risk factors in Bukhansan and Seoraksan National Park, which show similar characteristics, based on the trail safety evaluation data collected from the previous studies. In Bukhansan National Park, risk factors due to safety accidents such as fall and slipping are very high, whereas in Seoraksan National Park, the distribution of risk factors for falling rocks is higher than safety accidents. These results improved the existing standardized policy direction for trail safety management and derived the necessity of establishing subdivided standards according to environmental factors such as geology, topography, and visitor characteristics, even in national parks with similar environments. This study is expected to contribute to the improvement of trail safety management technology, enhancement of expertise, and prevention of damage to human life and property due to systematic safety management.

LC-MS Determination and Bioavailability Study of Imidapril Hydrochloride after the Oral Administration of Imidapril Tablets in Human Volunteers

Yun Ji Hye,Myung Ja Hye,Kim Hye Jin,Lee Sibeum,Park Jong-Sei,Kim Won,Lee Eun-Hee,Moon Cheol Jin,Hwang Sung-Joo The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.4

The purpose of the present study was to develop a standard protocol for imidapril hydrochloride bioequivalence testing. For this reason, a specific LC-MS method was developed and validated for the determination of imidapril in human plasma. A solid-phase extraction cartridge, $Sep-pak^{R}$ C18, was used to extract imidapril and ramipril (an internal standard) from deproteinized plasma. The compounds were separated using a XTerra $MS^{R}$?C18 column ($3.5 {\mu}m, 2.1\times150 mm$) and $acetonitrile-0.1\%$ formic acid (67:33, v/v) adjusted to pH 2.4 by 2 mmol/L ammonium formic acid, as mobile phase at 0.3 mL/min. Imidapril was detected as m/z 406 at a retention time of ca. 2.3 min, and ramipril as m/z 417 at ca. 3.6 min. The described method showed acceptable specificity, linearity from 0.5 to 100 ng/mL, precision (expressed as a relative standard deviation of less than $15\%$), accuracy, and stability. The plasma concentration-versus-time curves of eight healthy male volunteers administered a single dose of imidapril (10 mg), gave an $AUC_{12hr}$ of imidapril of $121.48\pm35.81 ng mL^{-1} h$, and $C_{max} and T_{max}$ values of $32.59\pm9.76 ng/mL and 1.75\pm0.27 h$. The developed method should be useful for the determination of imidapril in plasma with sufficient sensitivity and specificity in bioequivalence study.