Comparison of trophic factors changes in the hippocampal CA1 region between the young and adult gerbil induced by transient cerebral ischemia.

Yan, Bing Chun,Park, Joon Ha,Kim, Sung Koo,Choi, Jung Hoon,Lee, Choong Hyun,Yoo, Ki-Yeon,Kwon, Young-Geun,Kim, Young-Myeong,Kim, Jong-Dai,Won, Moo-Ho Kluwer Academic/Plenum Publishers 2012 Cellular and molecular neurobiology Vol.32 No.8

<P>In the present study, we investigated neuronal death/damage in the gerbil hippocampal CA1 region (CA1) and compared changes in some trophic factors, such as brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF), in the CA1 between the adult and young gerbils after 5 min of transient cerebral ischemia. Most of pyramidal neurons (89%) were damaged 4 days after ischemia-reperfusion (I-R) in the adult; however, in the young, about 59% of pyramidal neurons were damaged 7 days after I-R. The immunoreactivity and levels of BDNF and VEGF, not GDNF, in the CA1 of the normal young were lower than those in the normal adult. Four days after I-R in the adult group, the immunoreactivity and levels of BDNF and VEGF were distinctively decreased, and the immunoreactivity and level of GDNF were increased. However, in the young group, all of their immunoreactivities and levels were much higher than those in the normal young group. From 7 days after I-R, all the immunoreactivities and levels were apparently decreased compared to those of the normal adult and young. In brief, we confirmed our recent finding: more delayed and less neuronal death occurred in the young following I-R, and we newly found that the immunoreactivities of trophic factors, such as BDNF, GDNF, and VEGF, in the stratum pyramidale of the CA1 in the young gerbil were much higher than those in the adult gerbil 4 days after transient cerebral ischemia.</P>

연령증가에 따른 혈압요소들의 좌심실비대 및 관동맥질환과의 관련성

박재석,박창규,박미영,박재형,김용현,나진오,신성희,서순용,홍순준,박성미,임홍의,김응주,서홍석,오동주,노영무 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

지체장애인 편의시설의 실태조사 및 현황 분석에 관한 연구 : 대구광역시 종합사회복지관을 중심으로 Focusing of The Welfare Facilities in Daegu

최영오,박승환,최무혁 대한건축학회 2004 대한건축학회논문집 Vol.20 No.7

This study is to search for appearance of facilities based on facility improvement and then analyzes degree of satisfaction of user and level of facilities by comparing. The way of improvement of welfare facilities by searching can be summarized as follow : Facilities for access : For the handicapped, circulation of lamp should be separated from parking area. Parking area for the handicapped should be made safe against general people and also should be made guide equipments for the handicapped parking area. Facilities for shifting : Hand-rails that exist corridors and stairs should be founded with law of facility improvement. Welfare facilities that founded elevators should be slowed down closing time of elevator door for the handicapped, and welfare facilities that founded wheelchair lifts should be enhanced application by educating. Facilities for sanitation. : Avaliable close-stool space and avaliable door width of toilet should be founded by law of improvement facility. Floor tiles of toilet should be finished off non-slipping tiles. In conclusion, criteria of system are most important of all when facilities for the handicapped are founded. And it would be extremely ideal, if facilities can be designed appropriately for the handicapped with this criteria of system.

Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

3차원 전기비저항 역산 방법을 이용한 삼보 광산에서 금광 탐사

박종오 ( Jong Oh Park ),김희준 ( Hee Joon Kim ),송무영 ( Moo Young Song ),유영준 ( Young June You ) 대한지질공학회 2005 지질공학 Vol.15 No.1

전라남도 무안군 해제면에 위치한 삼보광산은 편마암류 및 유문암 내에 발달한 열극에 열수가 충전하여 형성된 열극충전광산이다. 시추 자료에서 얻은 분석 품위는 금이 0.05~10.9 g/t 이하, 은이 0.05~389 g/t 이하로 부분적으로 금·은광화작용이 인지되었다. 본 연구는 지표에서의 쌍극자, 슐럼버저 및 웨너 배열을 이용한 전기비저항 탐사와 시추공에서의 전기비저항 토모그래피 탐사를 복합하여 삼보광산에서 광화대의 분포 및 이와 관련된 지질구조를 파악하고자 한다. 3차원 전기비저항 역산의 결과는 광화대가 지표부터 약 80m 심도까지 30m의 폭을 가지며, N10°~20°E의 방향으로 240m 연장되어 있음을 보여준다. 전기비저항 영상에서 얻어진 1,000ohm-m 이하의 전기비저항 값은 시추 자료에서 확인된 금·은광화작용이 인지된 구간 및 연약대 구간과 거의 일치하였다. The Sambo mine is located in Hae-je Myeon, Moo-an Gun, Chollanamdo, which consists of host gneiss and rhyolite possessing quartzite veins with other compositions such as gold, silver, and sublimated sulfur. The ore grade estimated from the core was 0.05~10.9g/t or less in gold and 0.05~389g/t or less in silver, indicating a partial mineralization. The purpose of this paper is to understand the subsurface structures and the distribution of mineralized bodies in the Sambo mine using a combined method of Schlumberger, Wenner, and Dipole-dipole resistivity surveys on the surface and the resistivity tomography survey in boreholes. The result of three-dimensional resistivity inversion showed that the mineralized body is extended to 240m long in the N10°~20°E direction, with 30m wide and 80 m thick from the surface. The low resistivity zones (<1,000ohm-m) determined from the resistivity image were in good agreement with the mineralized bodies and weak zones identified from the logged cores.

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

노안수술후 각막형태검사 변화

박근성,고길환,김용선,이재림,민병무,최시환 충남대학교 의학연구소 2001 충남의대잡지 Vol.28 No.2

We prospectively analyzed the corneal topography of 26 eyes in 26 patients who had undergone scleral expansion for correcting presbyopia. We examined 26 eyes of 26 patients which were devided into 2 groups (mean age: 51 years, range 40-60 years) according to type of scleral expansion band, with computerized corneal topographic analysis system. Preoperatively and 5 times postoperatively (1day, 1week, 2weeks, 1month, 2months), quantitative descriptors of corneal topograpgy such as Sim K (simulated keratometry value), SRI(surface regularity index), and SAI(surface asymmetry index) were measured at each visit. The mean follow-up period was 43 days. Sim K of the operative eyes ranged from 0 to 2.5 (mean, 0.93) before operation, and mean 2.24 at postoperative 1 day in group Ⅰ. In group Ⅱ, Sim K was mean 2.20 at postoperative 1 day and decreased thereafter. SRI was from 0.54 to 1.39 and SAI was from 0.09 to 0.45 before operation. After operation, mean SAI was maximum at postoperative 2 week and mean SRI was maximum at postoperative 1 day and decreased thereafter. After two months, it became minimum. This study demonstrates that quantitative topographic change after scleral expansion for correcting presbyopia were great during first 1 week after surgery but this change diminished thereafter. Further long studies are in progress to evaluate the long term effects of this procedure.

국내에서 분리된 canine parvovirus의 구조유전자 cloning과 염기서열 분석

박종현,송재영,이중복,현방훈,안수환,전무형 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

In this study gene encoding structural proteins of a CPV isolate was cloned and saquenced to elucidate the molecular genetical properties of the canine parvoviruses isolated from the field. Six recombinant plasmids of pEP3, p1471, p2070, pEP069, pEP338 and p14711p were constructed from the map positions 22 to 98 of RF DNA to clone the VP1 and VP2 genes of CPV-V20. Sequentialy the gene comprising 3780 nucleotides were sequenced by dideoxy chain termination method. When nucleotide sequence of gene encoding the structural proteins of CPV-V20 was compared with those of other strains, CPV-N, CPV-d and CPV-780929 published previously. DNA homologies to CPV-V20 were 99.87% with CPV-NM, 99.73% with CPV-d, 96.85% with CPV-780929 AND 98.4% with FPLV-Carl, respectively. The DNA sequence data of CPV-V20 showed seven point mutations and also deletion of 135 nucleotides from the nucleotide position 4745 to 4879 located in the 3-noncoding region of CPV-N.

개에서 직장탈의 수술적 치료 1례

박정희,김영실,신영지,박창식,전무형,김명철,정성목 忠南大學校 獸醫科大學 動物醫科學硏究所 2005 動物醫科學硏究誌 Vol.13 No.-

A 3-year old, male Jindo dog was referred with history of tenesmus, dyschezia and recurrent rectal prolapse. On the physical examination, the rectum was prolapsed about 10 cm, congested and necrotized. Fecal incontinence and pain were observed. Complete blood count revealed a mild leukocytosis and there were no remarkable abnormalities in serum biochemistry profiles. It was diagnosed as a rectal prolapse. Rectal resection and anastomosis was performed for necrotic tissue removal. On postoperative fecal smear, protothecosis was observed. Amphotericin B was administered. There has been no evidence of recurrence of rectal prolapse for ten months.

Vibrio cholerae non-01/0139복막염 1예

박도심,이영진,김신무,조지현 대한임상미샐물학회 2000 Annals of clinical microbiology Vol.3 No.2