자연과학적 데이터를 활용한 풍납토성 백제토기의 분류 방안 연구

문은정 ( Eun Jung Moon ),강희준 ( Hee Jun Kang ),한민수 ( Min Su Han ),이한형 ( Han Hyoung Lee ) 백제학회 2011 백제학보 Vol.0 No.5

본 연구는 풍납토성 출토 백제토기를 대상으로 현장에서 소성온도에 따라 토기를 분류할 수 있는 외형관찰 기준을 마련하는데 자연과학적 데이터를 활용하고자 하는 연구이다. 소성온도는 토기의 경도, 밀도 풍을 포함한 다양한 물리화학적 특성에 영향을 미친다. 이 중 현장에서 측정이 가능하고 유용한 것이 경도이다. 그러므로 우리나라의 발굴현장에서 적용하고 있는 경도 측정기준을 적용하여 토기를 분류하고, 자연과학적 분석을 통하여 이들 토기의 마이크로비커스 경도, 밀도, 흡수율, 조직의 유리질화도, 광물학적 특성 등을 측정하여 현장에서 적용하는 경도분류 기준과의 상관관계를 비교연구하였다. 그 결과, 발굴현장에서 동전의 긁힘에 의해 주로 행해지던 연질과 경질의 이분법적 분류는 연질과 연경질, 경질의 세 단계로 세분됨을 알 수 있었다. 소성 온도 추정 결과, 연질은 1,000˚C 이하, 연경질은 1,000~1,100˚C, 경질은 1,200˚C 이상에서 소성되었을 것으로 판단된다. 한편, 3단계로 분류되는 토기들의 각 단계별 외형적 공통점을 추출하여 자연과학적인 정밀분석 없이 육안관찰만으로 토기를 3단계로 분류할 수 있는 관찰기준의 제시를 시도하였다. 이러한 기준은 파괴분석을 하지 않고도 토기의 외형적 특징을 이용하여 토기의 대략적인 물성과 소성온도 추정을 가능하게 할 것으로 기대된다. 단, 이러한 기준은 풍납토성에서 출토된 한성백제시기의 토기 중에서 배류와 호류 등 색상이 단조로운 제한된 기종을 중심으로 한 결과라는 한계를 가진다. 따라서 추가적으로 여러 기종의 다양한 색상을 가진 토기들을 대상으로 풍납토성 출토 토기의 경도별 분류가 가능하도록 수정·보완하는 연구를 진행할 예정이다. Observation methods of appearance were studied to establish a systemized classification criteria according to the firing temperatures of excavated earthenware from the Pung-Nap Mud Castle. A firing temperature has an impact on various physical and chemical properties including hardness and density of earthenware. Of all the properties, hardness can be measured in situ. Therefore, the earthenware were categorized according to the measurement method of hardness which can be applied and useful method for archaeological sites. In addition, the correlation between the measurement method and micro Vickers hardness, absorptance, degree of vitrescence and mineralogical properties of the earthenware were studied by means of a natural scientific analysis. The result suggests that the dichotomous way of classification based on a scratching method determining whether it is hard or soft should be categorized into three stages; soft, soft/hard and hard. According to the presumption of the firing temperature, it is believed that the earthenware were fired at below 1,000℃, 1,000~1,100℃ and 1,200℃ respectively. The systemized observation method was proposed to enable the earthenware to be categorized into three stages using visual observation without a detailed scientific analysis. The method will make it possible to estimate roughly the physical properties and firing temperature of the earthenware with only visual observation. However, it has limits as it was established focusing on certain types such as Cups and Jars among the Han-Sung Baek-Je earthenware. Therefore, a further study is planned to be carried out on various objects excavated from Pung-Nap Mud Castle in order to enable categorization on the basis of hardness of the earthenware.

한강둔치에서 재배된 농작물 중의 미중금속 함량에 관한 연구

김연천,전옥경,양혜란,최영희,한선희,이강문,Kim, Youn-Choen,Chun, Ock-Kyoung,Yang, Hae-Ran,Choi, Young-Hee,Han, Sun-Hee,Lee, Kang-Moon 한국식품위생안전성학회 2000 한국식품위생안전성학회지 Vol.15 No.3

한강 고수부지 및 지천인 안양천, 탄천, 중랑천변의 경작지에서 채집된 36종 285건의 농작물에 대한 Pb, Cr, Cd, Fe, Zn, Mn, Cu. Hg의 농도를 측정하고 그 결과를 채집 시기, 채집 지역 및 작물별로 비교, 분석한 결과, 작물 중의 중금속 함량은 평균치를 기준으로 볼 때 Fe(34.592 ppm)>Mn(11.071 ppm)>Zn(8.853 ppm)>Cu(1.795 ppm)>Cr(0.237 ppm)>Pb(0.100 ppm)>Cd(0.011 ppm)>Hg(0.003 ppm) 순으로 곡류 및 두류에서는 Zn의 함량이 Mn보다 높고, 채소류와 과실류에서는 Mn의 함량이 Zn보다 높은 양상을 나타냈다. 또한 각 지역에서 채취한 작물의 미량금속 함량의 경우 Fe은 0.004∼203.083 ppm, Cu은 0.017∼22.727 ppm. Mn은 0.000∼74.373 ppm. Zn은 0.080∼37.166 ppm으로 비교적 고농도로 존재하고 있는 반면 유해중금속류인 Pb은 0.000∼0.654 ppm, Cd은 0.000∼0.270 ppm, Cr은 0.000∼l.229 ppm, Hg은 0.000∼0.037 ppm으로 비교적 미량으로 존재함을 알수 있었다. 대상 농작물을 잎, 줄기, 뿌리, 열매로 분류하여 미량금속 함량을 분석한 결과, 분석 대상 금속의 농도가 잎>줄기>뿌리>열매의 순으로 나타났다. 무등의 뿌리 작물에 있어서 Pb은 잎(0.055 ppm))뿌리(0.035 ppm), Cr은 잎(0.118 ppm))뿌리(0.031 ppm), Cd은 잎(0.004 ppm)1뿌리(0.001 ppm), Hg은 잎(0.004 ppm))뿌리(0.001 ppm)으로 뿌리보다 잎에 많은 것으로 나타났는데 이러한 결과로 보아 뿌리는 금속 이온이 흡수되는 기관일 뿐 축적은 잎에 비해 상대적으로 적게 됨을 알 수 있었다. This study was conducted to determine the content of trace metals in crops cultivated in Han-riverside, Anyang stream, Tan stream, and Jungryang stream. Trace metals (Pb, Cr, Cd, Fe, Zn, Cu, Hg) were detected in 36 crops, 285 samples by Atomic Absorption Spectrophotometer and Mercury Analyzer. The average concentration of trace metals in crop samples was in the order of Fe (34.592 ppm)>Mn (11.071 ppm)$\geq$Zn(8.853 ppm)>Cu(1.795 ppm)>Cr(0.237 ppm)>Pb(0.100 ppm)>Cd(0.011 ppm)>Hg(0.003 ppm). In crop samples, the contents of Fe, Cu, Mn, and Zn, which ranges were 0.004-203.083 ppm, 0.017~22.727 ppm, 0.000~74.373 ppm, 0.080~37.166 ppm, respectively, were relatively higher than those of Pb, Cd, Cr, and Hg, which ranges were 0.000~0.654 ppm, 0.000~0.270 ppm, 0.000~l.229 ppm, 0.000~0.037 ppm, respectively. The concentration of trace metals in crop samples was in the order of leafy vegetables > stem vegetables > root vegetables > fruity vegetables. In the root vegetables, such as radish, the content of Pb was leaf(0.055 ppm)>root(0.035 ppm), that of Cr, leaf (0.118 ppm)>root(0.031 ppm), that of Cd, leaf(0.004 ppm)>root(0.001 ppm), that of Hg, leaf(0.004 ppm)>root(0.001 ppm). As the results, it could be thought that root is the organ which doesn't accumulate the heavy metal ions, but absorb them.

Phosphorylation of Hepatitis C Virus RNA Polymerases Ser29 and Ser42 by Protein Kinase C-Related Kinase 2 Regulates Viral RNA Replication

Han, Song-Hee,Kim, Seong-Jun,Kim, Eun-Jung,Kim, Tae-Eun,Moon, Jae-Su,Kim, Geon-Woo,Lee, Seung-Hoon,Cho, Kun,Yoo, Jong Shin,Son, Woo Sung,Rhee, Jin-Kyu,Han, Seung Hyun,Oh, Jong-Won American Society for Microbiology 2014 Journal of virology Vol.88 No.19

<P>Hepatitis C virus (HCV) nonstructural protein 5B (NS5B), an RNA-dependent RNA polymerase (RdRp), is the key enzyme for HCV RNA replication. We previously showed that HCV RdRp is phosphorylated by protein kinase C-related kinase 2 (PRK2). In the present study, we used biochemical and reverse-genetics approaches to demonstrate that HCV NS5B phosphorylation is crucial for viral RNA replication in cell culture. Two-dimensional phosphoamino acid analysis revealed that PRK2 phosphorylates NS5B exclusively at its serine residues <I>in vitro</I> and <I>in vivo</I>. Using <I>in vitro</I> kinase assays and mass spectrometry, we identified two phosphorylation sites, Ser29 and Ser42, in the Δ1 finger loop region that interacts with the thumb subdomain of NS5B. Colony-forming assays using drug-selectable HCV subgenomic RNA replicons revealed that preventing phosphorylation by Ala substitution at either Ser29 or Ser42 impairs HCV RNA replication. Furthermore, reverse-genetics studies using HCV infectious clones encoding phosphorylation-defective NS5B confirmed the crucial role of these PRK2 phosphorylation sites in viral RNA replication. Molecular-modeling studies predicted that the phosphorylation of NS5B stabilizes the interactions between its Δ1 loop and thumb subdomain, which are required for the formation of the closed conformation of NS5B known to be important for <I>de novo</I> RNA synthesis. Collectively, our results provide evidence that HCV NS5B phosphorylation has a positive regulatory role in HCV RNA replication.</P><P><B>IMPORTANCE</B> While the role of RNA-dependent RNA polymerases (RdRps) in viral RNA replication is clear, little is known about their functional regulation by phosphorylation. In this study, we addressed several important questions about the function and structure of phosphorylated hepatitis C virus (HCV) nonstructural protein 5B (NS5B). Reverse-genetics studies with HCV replicons encoding phosphorylation-defective NS5B mutants and analysis of their RdRp activities revealed previously unidentified NS5B protein features related to HCV replication and NS5B phosphorylation. These attributes most likely reflect potential structural changes induced by phosphorylation in the Δ1 finger loop region of NS5B with two identified phosphate acceptor sites, Ser29 and Ser42, which may transiently affect the closed conformation of NS5B. Elucidating the effects of dynamic changes in NS5B phosphorylation status during viral replication and their impacts on RNA synthesis will improve our understanding of the molecular mechanisms of NS5B phosphorylation-mediated regulation of HCV replication.</P>

Clonogenic Assay of Gastric Adenocarcinoma Stem Cells -Clonogenic Assay, Stomach Cancer

Hee Sook Moon,Boo Sung Kim,Dong Jip Kim,Hoon Kyo Kim,Han Lim Moon,Kyung Shik Lee 대한내과학회 1987 The Korean Journal of Internal Medicine Vol.2 No.2

Application of the Fuzzy Set Theory to Uncertain Parameters in a Countermeasure Model

Han,Moon-Hee,Kim,Byung-Woo 대한방사선 방어학회 1994 방사선방어학회지 Vol.19 No.2

원자력시설의 비상사태시 주변주민을 보호하기 위한 비상대응행위의 효과를 평가할 필요가 있다. 비상대응행위는 지역특성과 개인 행동특성에 따라 매우 다양하게 나타날 수 있으므로, 비상대응행위를 나타내는 변수들은 특정 값을 갖기보다는 일정구간에 분포되는 값을 갖는다. 대부분의 기존 비상대응모델에서는 계산을 단순화시키기 위하여 가정을 통해 특정값을 사용한다. 단순화 과정중에 필연적으로 정보의 손실이 발생되어 결과적으로 비상대응 모델은 큰 불확실성을 포함하게 된다. 퍼지이론은 변수의 불확실성을 계산에 포함시켜 엄밀한 계산을 통해 정보손실을 초소화시키면서 계산결과를 얻어낼 수 있는 수학적인 도구를 제공해 준다. 본 연구에서는 퍼지집합, 퍼지추론, 퍼지관계등의 이론을 응용하여 원자력시설의 비상사태시 비상대응효과를 평가할 수 있는 방법을 개발하였다. 개발된 모델의 장점은 언어변수를 이용하여 지역특성을 표현하고 전문가의 의견을 반영하여 비상대응효과를 평가하므로, 단순한 가정중에 유발되는 정보의 손실을 줄일 수 있는데 있다. 비상대응 모델내의 불확실한 변수에 대한 퍼지이론의 응용성을 개선하기 위해서는 전문가의 의견을 반영하여 변수들에 대한 적합한 멤버쉽 함수와 퍼지조건문을 확립할 필요가 있다. A method for estimating the effectiveness of each protective action against a nuclear accident has been proposed using the fuzzy set theory. In most of the existing countermeasure models in action under radiological emergencies, the large variety of possible features is simplified by a number of rough assumptions. During this simplification procedure, a lot of information is lost which results in much uncertainty concerning the output of the countermeasure model. Furthermore, different assumptions should be used for different sites to consider the site specific conditions. In this study, the diversity of each variable related to protective action has been modelled by the linguistic variable. The effectiveness of sheltering and evacuation has been estimated using the proposed method. The potential advantage of the proposed method is in reducing to loss of information by incorporating the opinions of experts and by introducing the linguistic variables which represent the site specific conditions.

Acute and 28-Day Subacute Toxicity Studies of Hexane Extracts of the Roots of Lithospermum erythrorhizon in Sprague-Dawley Rats

Han, Chung-Tack,Kim, Myoung-Jun,Moon, Seol-Hee,Jeon, Yu-Rim,Hwang, Jae-Sik,Nam, Chunja,Park, Chong-Woo,Lee, Sun-Ho,Na, Jae-Bum,Park, Chan-Sung,Park, Hee-Won,Lee, Jung-Min,Jang, Ho-Song,Park, Sun-Hee,H Korean Society of ToxicologyKorea Environmental Mu 2015 Toxicological Research Vol.31 No.4

Lithospermum erythrorhizon has long been used as a traditional oriental medicine. In this study, the acute and 28-day subacute oral dose toxicity studies of hexane extracts of the roots of L. erythrorhizon (LEH) were performed in Sprague-Dawley rats. In the acute toxicity study, LEH was administered once orally to 5 male and 5 female rats at dose levels of 500, 1,000, and 2,000 mg/kg. Mortality, clinical signs, and body weight changes were monitored for 14 days. Salivation, soft stool, soiled perineal region, compound-colored stool, chromaturia and a decrease in body weight were observed in the extract-treated groups, and no deaths occurred during the study. Therefore, the approximate lethal dose (ALD) of LEH in male and female rats was higher than 2,000 mg/kg. In the subacute toxicity study, LEH was administered orally to male and female rats for 28 days at dose levels of 25, 100, and 400 mg/kg/day. There was no LEH-related toxic effect in the body weight, food consumption, ophthalmology, hematology, clinical chemistry and organ weights. Compound-colored (black) stool, chromaturia and increased protein, ketone bodies, bilirubin and occult blood in urine were observed in the male and female rats treated with the test substance. In addition, the necropsy revealed dark red discoloration of the kidneys, and the histopathological examination showed presence of red brown pigment or increased hyaline droplets in the renal tubules of the renal cortex. However, there were no test substance-related toxic effects in the hematology and clinical chemistry, and no morphological changes were observed in the histopathological examination of the kidneys. Therefore, it was determined that there was no significant toxicity because the changes observed were caused by the intrinsic color of the test substance. These results suggest that the no-observed-adverse-effect Level (NOAEL) of LEH is greater than 400 mg/kg/day in both sexes.

Analysis of Radiation Exposure from Nuclear Reactor Accident in Complex Terrain

Moon Hee Han,Sung Ki Chae,Moon Hyun Chun Korean Nuclear Society 1985 Nuclear Engineering and Technology Vol.17 No.3

원자력 발전소로부터 방출되는 기체상 방사성 물질에 의한 환경 영향을 평가함에 있어서 방사성 물질의 대기중에서의 수송과 확산을 기술하는 모델로써 Gaussian plume mode띠 널리 사용되고 있다. Gaussian plume model은 평탄한 지형에 적용하도록 만들어진 모델이므로 대부분의 국토가 복잡한 산악으로 구성된 한극의 경우에 적용하기 위해서는 모델의 수정이 필요하다. 본 논문에서는 2차원적 x-z 평면에서 확산방정식을 해석한 numerical diffusion model과 Gaussian plume model을 비교하여, Gaussian plume mode에서 가장 중요한 변수인 dispersion coefficient를 지형의 높이에 대하여 보정하였다. 보정된 dispersion coefficient 값을 Gaussian plume mode에 적용시켜 계산을 수행한 결과를 보면, 산악지역에서의 방사성 물질의 농도는 평지에서보다 낮게 나타나고 있다. The Gaussian plume model is widely used to calculate the concentrations of gaseous radioactive effluents in the atmosphere. This model assumes that the terrain is flat, so that the dispersion coefficients which are the most important parameters in this model must be compensated in complex terrain such as in Korea. In this study the compensation of vertical dispersion coefficient in two dimensional x-z plane has been accomplished by comparing the Gaussian plume model with numerical model. The results show that the concentractions of radioactive effluents over complex terrain are more dilluted than those expected over flat terrain.

Comparison of Radiation Exposures from Coal-fired and Nuclear Power Plants

Han, Moon-Hee,Kim, Byung-Woo,Yoo, Byung-Sun,Lee, Jeong-Ho Korean Nuclear Society 1987 Nuclear Engineering and Technology Vol.19 No.2

Comparison study on the radiological effects by radionuclides from hypothetical 1,000MWe coal-fired power station and nuclear power plant is made. This paper describes the radiological effects only for gaseous effluents released in normal operation. Source terms for coal-fired Power station are quoted from foreign data and those for nuclear power plant are calculated for reference power plant. Gaussian plume model is used to assess atmospheric dispersion of radioactive effluents based on one year meteorological data of Kori site and individual doses are calculated at the maximum X/Q point. Doses from nuclear power plant are slightly more than those from coal-fred power plant. In the case of coal-fired power plant, doses by ingestion of contaminated vegetation are 73.5% of total doses.

Protective Effect of Carvacrol on Acute Alcohol-Induced Liver Disease Model in Mice

Moon Han Chang,Bo Byeol Hwang,Ji Eun Moon,Eun Hee Jo,Min Kook Lee,Seoyoon Lee,Suk Hee Lee,Hanvit Cha,Jin Hyup Lee 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

Quantitative Analysis of Meteorological Conditions for a Comparative Risk Estimation of Nuclear Power Plants in Korea

Han, Moon Hee,Hwang, Won Tae,Jeong, Hyo Joon,Kim, Eun Han Informa UK (Taylor Francis) 2008 Journal of nuclear science and technology Vol.45 No.suppl5