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      • 콘크리트의 급속염화물침투시험에 의한 염화물이온 확산계수 평가에 관한 실험적 연구

        김갑수,조봉석,장종호,김재환,김용로,김무한 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

        Chloride ions are considered to be the major cause of steel corrosion in concrete structures exposed to seashore environments and also permeation of chloride is controlled by chloride diffusion. Therefore, the study on chloride diffusion of concrete have been done so far by many researchers. In this study, several factors such as capacity of voltage, time of an electric current, concentration of NaCl solution, water-cement ratio, and temperature of the solution were examined to grasp what influence they were on the chloride ion diffusion properties using the Rapid Chloride Penetration Test(RCPT) by electrophoresis. Also standing on the basis of the such result application possibility of Rapid Chloride Penetration Test(RCPT) used in this study will be evaluated and the basic data on the durability design for the chloride attack will be suggested.

      • PVA-ECC를 활용한 Half PC 복합체의 역학적 특성에 관한 실험적 연구

        김재환,김영덕,김갑수,조봉석,장종호,김무한 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2

        ECC represents one particular class of HPFRCC, which are defined by an ultimate strength higher than their first cracking strength and the formation of multiple cracking during the inelastic deformation process. This study is to provide the fundamental data for the development of hybrid construction method such as the half PC(Precast concrete) reinforced by PVA-ECC, the mechanical properties of half PC according to the alteration of properties of PVA-ECC in terms of the type of fiber, Vr, S/M, placing height of PVA-ECC, are experimentally investigated. As the results. it is found that the half PCs of PVA-ECC have very higher mechanical performance in terms of yield load, strain capacity beyond yield point and maximum flexural load than that of mortar. Also, flexural crack in the half PCs of PVA-ECC are not localized at first crack and are growed to many micro crack, especially multiple cracking in specimen of P(12)-30-0.1ㆍ3 occurred.

      • 뿜칠시공에 의한 단면복구재의 시공성 및 공학적 특성에 관한 실험적 연구

        조봉석,김영선,장재봉,김용로,김경덕,김무한 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

        These days, serious concerns of reinforced concrete have been raised recently over steel-bar corrosion, cracking, the deterioration of durability and structural stress resistance and so on, all due to the effects of exterior factors. Such being the case, in domestic, various repair materials and method systems to keep up with these reinforced concrete deteriorated due to salt damage, carbonation, chemical decay et. developed and applied. However, on-site quality control of various repair materials and method systems isn't achieved desirably because it is depend completely on a men of experience' opinions above all else regardless of various on - site environments. In this background, mock up test with due regard to real on-site environments was performed to secure fundamental data for establishment of desirable on-site quality control. Mock up test using repair mortar analyzed from angles of construction methods, mechanical spraying pressures, W 1M. Construction methods were designed manpower method and spraying method, spraying pressures were designed 32, 42, 52 psi, W/M were designed 14.4, 15.4, 16.4 %. And compressive strength, Chloride ion diffusion coefficient, bond strength, SEM of mock up test specimens were evaluated. In conclusion, we confirmed excellency of mechanical spraying pressures, fined extremely excellency of condition of spraying pressure 42 ps, W/M 14.4% within this study. therefore the results of this study will be useful to provide fundamental data for establishment of desirable on-site quality control.

      • モルタルおよびコンクリ-トの積算溫度と壓縮强度發現に關する實驗的硏究

        金武漢,洪悅郞,鎌田英治 忠南大學校 産業技術硏究所 1986 산업기술연구논문집 Vol.1 No.1

        An experimental study with regard to maturity and compressive strength development of concrete was carried out by using mortar and concrete specimens. Eight kinds of normal portland cement and two from Hokkaido distret were used for motar and concrete respectively. On the basis of statistical calculation of this expernmental data. the following observations and conclusions seem to be appropmate. 1) The relation between logarithmic value of maturity and compressive strength development of martar and concrete expressed in cubic regression equation shows a good correlation. 2) Since the practical curves for compressive strength development below 210D·D shown in JASS were intended to give lower limit, the obtained curves based on this test results are higher in the range below 210D·D and the curves in the range above 210D·D show lower value of concrete strength. 3) The standard additional temperature for calulation of maturity shows a little discrepancy by kinds of cement and water cement ratios, however it seems to be practically appropriate to take 10℃ hitherto as the standard additional temperature.

      • 결장직장 암종에서 Brain Type Glycogen phosphorylase 발현에 대한 면역조직화학적 연구

        김수한,정동준,이정은,백무준,김창진 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

        The colorectal carcinoma shows the highest incidence of cancer death in Korea. The colorectal carcinomas are diverse in their carcinogenesis, as some progress from polypoid adenoma to carcinoma while others occur de novo, or combined de novo and adenoma to carcinoma sequence. The searchs for the early biomarker of the colorectal carcinoma are important not only for the early detection of the cancer but also for the effective treatment. Brain type glycogen phosphorylase (BGP) is a kind of fetal isoenzyme frequently expressed in the various carcinoma, such as gastric carcinoma, renal cell carcinoma and colorectal carcinoma. In this study, the expression of BGP was evaluated in the colorectal carcinoma, normal appearing mucosa adjacent to the carcinoma and aberrant crypts foci (ACF) by immunohistochemistry using 70 cases of colorectal carcinoma. The results were as follows. 1. The BGP was expressed in 91.4% of 70 cases of colorectal carcinoma. 2. The expression rate of BGP accoarding to the location of the carcinoma was not different. 3. Seventeen percent of the colorectal carcinoma occurred by the polypoid adenoma-carcinoma sequence, while 60% of the cases occurred from de nove and 22.9% from combined polypoid adenoma-carcinoma sequence and de nove. 4. The BGP was expressed in 91.4% of normal mucosa adjacent to the carcinoma, while in 17.1% of normal mucosa for apart from the carcinoma. The BGP expressed in the above results suggest that the colorectal carcinoma arsie more frequently in de novo than polypoid adenoma-carcinoma sequence, which suggest that the pathogenesis of coloretal carcinoma in Korean might be different from that of western people. And the expression pattern of BGP in the colorectal mucosa could be early biomarker of carcinoma and the immunohistochemical stain for the expression of the BGP might be used for the early detection of the colorectal carcinoma.

      • 三天池의 環境과 微生物의 多樣性

        한상국,이종운,배소현,장무웅,김시균 嶺南大學校 環境問題硏究所 1993 環境硏究 Vol.13 No.1

        경상북도 경산시에 위치하고 있는 삼천지에 대한 환경요인 및 미생물상에 관하여 조사하였다. 삼천지 수계의 환경요인 분석결과, 그 평균값은 기온이 25.8℃, pH6.77, 경도 117.94mg/ℓ, 부유물질 4.5mg/ℓ, 용존산소량 3.3mg/ℓ, 생물학적 산소요구량 6.6mg/ℓ, 화학적 산쇼요구량 7.2㎎/ℓ, 염소이온 17.56㎎/ℓ, 황산이온 7.14㎎/ℓ로 나타났고, 중금속류는 Mn과 Zn만이 소량 검출되었으나 전반적인 오염도가 높아 수질환경 기준 IV급수에 해당하였다. 총 세균수는 3.32-8.85×?? No. inds/㎖의 분포를 나타냈고, 식물플랑크톤의 종구성은 규조류 33종, 녹조류 26종, 남조류 8종, 유글레나 13종, 와편모조류 6종 등 86종이 확인되었다. 규조류중 띠돌말과에 속하는 Fragilaria capucina, Synedra acus, S. familiaris 가 우점종이였다. Environmental factors and microflora of Samchun-impoundment in Kyongsan, Kyongpook were studied. Average data of environmental factors analysis are as followings : air temperature 25.8℃, pH 6.77, total hardness 117.94mg/ℓ, SS 4.5mg/ℓ, DO 3.3mg/ℓ, BOD 6.6mg/ℓ, COD 7.2mg/ℓ, ?? 17.56mg/ℓ, ?? 7.14mg/ℓ, Mn 0.05mg/ℓ, Zn 0.04mg/ℓ. Total bacteria in 4 site were 3.32×?? No. inds/㎖. Distribution and species of phytoplankton and zooplankton were 86 species including 33 species of bacillariophyceae, 26 of chlorophyta, 8 of cyanophyceae, 13 of euglenaceae, 6 of dinophyceae. Fragilaria capucina, Synedra acus, S. familiaris were dominant species.

      • Brucella abortus 국내 분리주의 Heat Shock Protein 암호 groE 유전자의 염기서열 분석과 발현

        김태용,김지영,장경수,김명철,박창식,한홍율,전무형 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

        GroE that is a heat shock protein composed of GroEL and GroES is known as an immunodominant target of both the humoral and cellular immune responses in bovine brucellosis. This study was carried out to characterize groE gene encoding heat shock proteins of B. abortus isolated in Korea and to evaluate the immunogenicity of the GroE protein expressed in E. coli system. In PCR the specific signals with the size of 2,077 bp were detected in five strains isolated from the mammary lymphnodes of the dairy cattle that were serologically positive and the reference strains. In comparison of the sequences of nucleotides and amino acids among the strains, GroES showed 100% identity in both sequences. GroEL was evaluated 99.0∼99.9% in nucleotides and 98.0∼100% homology in amino acids. The groE gene including groES and groEL was inserted into pET29a vector and constructed pET29a-GroE recombinant plasmids. The inserted groE was confirmed by digestion with Ncol and EcoRl endonucleases and nucleotide sequencing. E. coli BL (DE3) was transformed with pET29a-GroE, named as E. coli BL (DE3)/pET29a-GroE. In SDS-PAGE, it was evident that the recombinant plasmid effectively expressed the polypeptides for GroES (10 kDa) and GroEL (60 kDa) in 0.5, and 2 hours after IPTG induction. The immuno-reactivity of the expressed proteins were proved in mouse inoculation and Western blot analysis.

      • 증점제를 사용한 고유동콘크리트의 기포조직과 내동해성에 관한 실험적 연구

        김무한,권영진,강석표,여동구,김용로,장종호 충남대학교 산업기술연구소 2000 산업기술연구논문집 Vol.15 No.1

        Recently, lots of studies for high flowing concrete have been suggested under practical use that it is only a way to solve the confronted problem. However, most studies have been concentrated on the manufacture method and properties of fresh concrete, but there is few studies for the durability of hardened concrete, specially for the freezing and thawing. It is generally known that the frost resistance of concrete is much affected by the air content in concrete and by the air void system. The frost resistance is also believed to vary with the strength of concrete. The purpose of this experimental study is to make a comparison between high flowing concrete using viscosity agent and ordinary concrete about the effect of the viscosity agent, air content and curing method on the frost resistance. As the result of tutus study, the frost resistance have not effect the viscosity agent but effect the different of curing methods.

      • 물결합재비 및 단위수량에 따른 고유동콘크리트의 제공학적 특성에 관한 실험적 연구

        김규용,한장현,신동주,신홍철,김무한 忠南大學校 産業技術硏究所 1998 산업기술연구논문집 Vol.13 No.1

        Recently the study on high flowing concrete which has high workability and non-compacting is being proceeded actively in the university and corporative laboratory. There are some cases that has been applied to the field. This high flowing concrete has higher fluidity and segregation resistance than Plain or flowing concrete. And it is being focused as a remarkable know-how which can make high-quality concrete and reduction effect of labor force. This properties of high flowing concrete are influenced by the relationship of several factors; binder content water binder ratio and unit water content. It is the aim of this study to propose reference data at mix design of high flowing concrete, after comparing and analyzing the fluidity and strength properties of high flowing concrete according to water binder content ratio and unlit water content.

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