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MPC 11 cell에 TPA 처리로 유도되는 DNA 결합 단백질의 특성
임계택,이정채 全南大學校 農業科學技術硏究所 1996 農業科學技術硏究 Vol.31 No.-
Vimentin의 전사인자(transcriptional factor)에 대한 기초자료로소 그 발현정도를 알아보기 위해 MPC11 cell에 Phorbol ester, 12-0-tetradecanoy phorbol 13-acetate (TPA)를 처리한 다음 DNA 결합 단백질을 세포질과 핵으로부터 분리하여 추출 한 후 gel mobility shift assay 방법으로서 그 성격을 연구했다. TPA를 처리한 MPC11 cell에서 처리 후 3시간에 단백질이 발현되었으며 그 단백질은 vimentin으로 추측된다. 한편 DNA 결합 단백질은 NF1과 TFN보다는 AP2에 대해 더 큰 결합력을 가지고 있는데 이는 AP2가 vimentin 발현과정에 있어 어떤 specific promotor element와 상호작용을 하는 것으로 여겨지며, TPA 의 처리에 의해서 vimentin의 발현이 촉진됨을 추측할 수 있었다.
鄭相澤,吳東燮,安禹洪,金正子,林銖遠 慶北大學校 體育科學硏究所 1991 체육과학연구지 Vol.7 No.-
This study was aimed to elucidata Taegu citizens' consciousness about Sport for All. 950 subjects responded to the researcher's questionnaires. The results are as follows; 1) 88.8% of whole subjects felt necessity of physical activity in every day life. In view of sex, 91.0% of male and 86.5% of female felt the necessity. 2) 73.4% of the subjects put weight in health improvement and 19.1% of the subjects put weight in strong mental power in relation to the value of physical activity. 3) About the local facilities of physical activity, only 8.3% of the subjects satisfied. A largy majority(65.7%) of the subjects reveated dissatisfaction. 4) Preference orders in relation to the expansion of facilities appeared as follows; tennis court(24.5%), swimming pool(14.4%), badminton court(12.5%), gymnasium(11.4%) and football field(7.0%). 5) 76.1% of the sujbjects thought that physical activity contribute to reduce demoralized life style, but 3.2% of the subjects not thought so. 6) About present participation in physical activity, 31.8% of the subjects answered being participated, but the large majority(68.2%) of the subjects answered not being participated. 7) Subjects' favorite physical activitier are tennis(18.6%), mounteering (12.9%), football(8.9%), golf(8.6%), badminton(8.3%), jogging(8.3%), gymnasics(7.3%) and swimming(7.0%) 8) The places of physical activities are private facilities(33.4%), work place(13.6%), private house(12.6%), school(10.9%) and physical education park(9.3%). 9) 73.9% of the subjects answered that they were participating in physical activity for health improvement. 10) 70.6% of the subjects felt the necessity of coach of Sport for All. 11) In case of not attendance to the physical activity, 70.6% of them answered that the main reason of not attendance was lack of leisure. 12) In case of not attendance, 33.7% of them spared time to TV watching, 28.8% of them to housework and 18.0% of them to sleeping. 13) 13.5% of subjects wanted to participate in swimming, 12.9% to tennis, 11.7% to table tennis, 10.8% to mountaineering, and 10.3% to badminton if circumstances permit.
Jeong, Seong-Yeop,Velmurugan, Palanivel,Lim, Jeong-Muk,Oh, Byung-Taek,Jeong, Do-Youn Elsevier 2018 FOOD SCIENCE AND TECHNOLOGY -ZURICH- Vol.93 No.-
<P>The aim of this study was to investigate if fermentation of blueberry fruit by Bacillus amyloliquefaciens and Lactobacillus brevis under green, red, blue, and white light emitting diode (LED) illumination or sunlight could yield functionalized products for biomedical applications. Fermentation by both bacterial species under white and green LED light and sunlight resulted in increased viable cell count of the bacteria as well as total phenol and flavonoid contents over 72 h of fermentation. Blue and red LED light fermentation and that in darkness resulted in fermented samples with moderate anti-bacterial activity based on minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) as well as antioxidant and cytotoxic assays compared to fermentations under other light sources. In addition, Fourier transform infrared spectroscopy (FT-IR) was performed to determine the functional groups in the non-fermented (control) and fermented products. Sunlight and white, red, and green LED light-fermented extracts contained alcohol, acid, and phenol groups, as well as aliphatic amines. The results of this study clearly indicate that fermentation of blueberries with probiotic bacteria under various LED light sources can yield compounds that can be used in cosmetic and value-added food products.</P>
Preparation and Characterization of Natural Material Extracted from Germinated Brown Rice
Lim, Ki-Taek,Choi, Jeong Moon,Lim, Won-Chul,Kim, Jangho,Cho, Hong-Yon,Chung, Jong Hoon Korean Society for Agricultural Machinery 2014 바이오시스템공학 Vol.39 No.3
Purpose: The aim of this study was to prepare and evaluate a natural material extracted from germinated brown rice (GBR). Herein, we evaluated whether the natural material could positively activate the biological effects seen during bone formation, including enhancement of metabolic activity, osteogenesis, and the expression of vascular endothelial growth factor (VEGF), one of the growth factors in human osteoblast-like cells. Methods: The natural material was created by a hot water extraction process after being soaked for 2~3 days in tap water and dried at $50^{\circ}C$. The material was characterized using field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and Fourier transformed infrared (FTIR) spectroscopy. The biological behaviors of the material were also investigated; we performed tests to assess cell cytotoxicity, metabolic activity, osteogenic markers related to bone formation, and VEGF. Results: The EDX, XRD, and FTIR results for the natural material indicated the presence of organic compounds. The natural material caused positive increases in cell metabolic activity and mineralized bone formation without cytotoxicity. The protein levels in the extract for the $6.25{\mu}g/mL$, $12.25{\mu}g/mL$, $25{\mu}g/mL$, $50{\mu}g/mL$, and $100{\mu}g/mL$ groups were significantly different from that for the control. Conclusions: The GBR-based natural material was easy to prepare and had characteristics of a potential biomaterial. The biocompatibility of this natural material was evaluated using in vitro techniques; our findings indicate that this novel material is promising for agricultural and biological applications.
박정영 ( Jeong Yeong Park ),이은주 ( Eun Ju Lee ),홍성수 ( Seong Soo Hong ),임권택 ( Kwon Taek Lim ),정용석 ( Yong Suk Jeong ),정연태 ( Yeon Tae Jeong ) 한국화상학회 2002 한국화상학회지 Vol.8 No.1
황산 발생제와 산에 민감한 고분자로 구성된 화학 중폭형 포토레지스트에 산 증식제를 첨가하면 산 증식제의 분해에 의한 산 촉매 분자의 중폭 현상 때문에 감도가 향상되는 결과를 얻을 수 있다. 우리는 photoresist 조성 공정을 간소화하고 광감도를 증가시키기 위해 tert-butyl methacrylaty와 4-methacryloyloxy-4-tosyloxy isopropylidene dicyclo-hexane을 공중합하여 고분자형·증식제에 기초한 positive working photoresist로써 poly(MTID_10-co-tBMA_90)를 개발했다. 이런 고분자형 산 증식제는 레지온도에 대하여 충분한 열적 안정성을 나타냈다. Poly(MTID_10-co-tBMA_90)는 이런 고분자의 곁사슬에 있는 산 증폭 기능을 하는 용기의 분해 결과로서 산 촉매 분자가 증폭되므로 광감도가 증진된다. Poly(MTID_10-co-tBMA_90)는 tBMA homopolymer에 비이상의 광감도 증진 효과를 나타내었다. The addition of the acid amplifier to a chemically amplified photoresist consisting of photoacid generator and acid-sensitive polymer results in the improvement of photosensitivity due to the amplified generation of catalytic acid molecules as a result of the decomposition of acid amplifiers. We developed a copolymer, poly(mtid_10-co-tBMA_90) , of tert-butyl methacrylate and 4-methanyloyloxy-4-tosyloxy isopropylidene dicyclohexane as a positive working photoresist based in polymeric acid amplifier in order to enhance photosensitivity and simplify the process of formulating photoresist. Poly(MTID_10-co-tBMA_90) had an improvement of photosensitivity due to the amplified generation catalytic acid molecules as a result of decomposition of the acid amplifying functional group in the side chain of this polymer. This polymeric acid amplifiers showed reasonable thermal stability for resist processing temperature. The poly(NTID_10-co-tBMA_90) exhibited 2X higher photosensitivity compared with tBMA homopolymer(PtBMA).