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정재현,이은지,이은주,황진원,송영진,최병진,김일환,김종윤,허민영,김동욱 인제대학교 2009 仁濟醫學 Vol.30 No.-
Neonatal lupus is a rare syndrome occurring In neonates with tranplacentally acquired maternal autoantibodies, In particular anti-SSA/Ro-SSB/Ra antibodies. Manifestations include congenital heart block, cutaneous lesions, thrombocytopenia, neutropenia, pulmonary or neurological disease and hepatitis. The non-cardiac manifestations resolve by 6 months of age, congenital heart block is irreversible. We experienced a case of neonatal lupus with congenital complete heart block in a newborn of asyptomatic mother with anti-SSA/Ro-SSB/Ra antibodies.
PCR을 이용한 국내 및 수입 축산물 유래 Listeria monocytogenes의 유전학적 분석연구
우용구,이수화,이철현,최정수,류재두,김영일,이오수,김봉환 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.2
One hundred-fifty two of Listeria strains were isolated from domestic chicken carcasses, a slaughter-houses from nationwide from 1996 to 1997 and imported livestock products from a ten foreign countries including the USA, China, France and Thailand. To substitute the laborious and time consuming procedures of clinical laboratory standard diagnostic methods, the rapid and specific multiplex PCR(M-PCR), which was designed to amplify a three kinds of genes simultaneously f3r specific detection and differentiation of L. monocytogenes(LM) by one step procedure, was established. The LM strains confirmed with M-PCR were analysed to their genetic diversities by RAPD using the two primers(D87 & MMTl), and also evaluated on their discriminatory abilities(na) between two primers. our study also conducted the REP-PCR and ERIC-PCR for evaluation of the possibility of REP and ERIC elements for genetic subtyping of LM strains. According to the REP-PCR and ERIC-PCR fingerprinting patterns, LM strains were divided into 7(ERIC-type) and 4(REP-type) major clusters at the relative clone cut off value of 80%. According to the computer analysis of PCR results using the GelCompar Ⅱ software, ERIC-PCR(DI=0.955) had expressed the most high discriminatory ability and followed by REP-PCR(DI=0.952), RAPD(D87; 0.954, MMT1; 0.937), in order. The combined analysis of the present PCR results was also expressed the reliable and useful discriminatory ability(DI=0.916). This study suggested that PCR based methods could be used as an reliable, reproducible, rapid, and highly discriminatory method for the genetic differentiation of genus Listeria.
Sensor Alignment Calibration for Precision Attitude Determination of Spacecrafts
Il-Hyoung Lee,Chang-Kyung Ryoo,Hyochoong Bang,Min-Jea Tahk,Sang-Ryool Lee 한국항공우주학회 2004 International Journal of Aeronautical and Space Sc Vol.5 No.1
A new alignment calibration method of attitude sensors for the precision attitude determination of a spacecraft based on the extended Kalman filter is proposed. The proposed method is divided into two steps connected in series: the gyro and the star tracker calibration. For gyro calibration, alignment errors and scale factor errors are estimated during the calibration maneuver under the assumption of a perfect star tracker. Estimation of the alignment errors of the star trackers and compensation of the gyro calibration errors are then performed using the measurements including payload information. Performance of the proposed method are demonstrated by numerical simulations.
Sensor Alignment Calibration for PrecisionAttitude Determination of Spacecrafts
Lee, Il-Hyoung,Ryoo, Chang-Kyung,Bang, Hyo-choong,Tahk, Min-Jea,Lee, Sang-Ryool The Korean Society for Aeronautical and Space Scie 2004 International Journal of Aeronautical and Space Sc Vol.5 No.1
A new alignment calibration method of attitude sensors for the precisionattitude determination of a spacecraft based on the extended Kalman filter is proposed.The proposed method is divided into two steps connected in series: the gyro and thestar tracker calibration. For gyro calibration, alignment errors and scale factor errorsare estimated during the calibration maneuver under the assumption of a perfect startracker. Estimation of the alignment errors of the star trackers and compensation ofthe gyro calibration errors are then performed using the measurements includingpayload information. Performance of the proposed method are demonstrated bynumerical simulations.
Deok Jea Cha,Ju Il Kim,Min Kwon,Yong Gyu Lee 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10
PVY (Potyviridae: potyvirus) is one of the most important potato virus affecting seed potato production and also it is transmitted non-persistently via aphids. For healthy seed potato production, a virus detection system is highly important in addition to aphid monitoring and control. To achieve this detection method, it need to fast and easy to use. About two decades ago RT-PCR based PVY detection method was developed. However that was very time consuming and has low sensitivity. Here, we developed an advanced PVY detection method which a uses the boiling extraction of the viral RNA from aphid stylet and amplification by specific primers located in the viral capsid protein gene. Therefore, it could directly synthesize cDNA of PVY viral capsid gene from extracted RNA of PVY using one-step RT-PCR method in very short time compared to previous methods due to the omission of RNA extraction step. We confirmed this PVY detection method using the two aphid species (Macrosiphum euphorbiae and Aphis gossypii) that known as PVY vectors. The efficiency of this PVY detection method was 60% to 80% from two the aphid species. Hence, this method could be potentially applied to virus free seed potato production programs.
pIN: A Novel Expression Vector from Ichnovirus
Ju Il Kim,Deok Jea Cha,Min Kwon,Si Hyeock Lee,Yonggyun Kim 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10
The genus Diadegma is a well known parasitoid group and some are known to have symbiotic virus, PDV. A novel IV was discovered from the calyx of D. fenestrale female. This virus was named as D. fenestrale Ichnovirus (DfIV). The encapsidated DfIV genome contains 65 circular DNA segments with an aggregate size of 247,191 bp. Based on BLAST analysis, a total of 120 ORFs were predicted as follows: rep; 48, cys-motif; 11, vinnexin; 10, vankyrin; 9, PRRP; 3 and other unassigned genes (39). These viral genes were expressed in lepidopteran hosts (Phthorimaea operculella and Plutella xylostella) after parasitization which means DfIV genome segments were integrated into lepidopteran hosts. This study was focused on this result. Based on gene expression profile, candidate promoter and integration motifs were selected and then, fused with eGFP as a reporter gene. Modified DfIV genome segment was ligated to a commercial containing f1 ori and Ampr gene to propagate in E. coli. We have named this fusion vector as pIN. The construction methodology of pIN and its application would be further discussed in detail.
이재준(Lee Jea Jun),최정일(Choi Jung Il),이필규(Lee Phill Kyu) 한국정보처리학회 1997 정보처리학회논문지 Vol.4 No.12
This paper addresses an eye location algorithm which is essential process of human face tracking system for natural video-conferencing. In current video-conferencing systems, user's facial movements are restricted by fixed camera, therefore it is inconvenient to users. We propose an eye location algorithm for automatic face tracking. Because, locations of other facial features guessed from locations of eye and scale of face in the image can be calculated using inter-ocular distance. Most previous feature extraction methods for face recognition system are approached under ssumption that approximative face region or location of each facial feature is known. The proposed algorithm in this paper uses no prior information on the given image. It is not sensitive to backgrounds and lighting conditions. The proposed algorithm uses the valley representation as major information to locate eyes. The experiments have been performed for 213 frames of 17 people and show very encouraging results.