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물곰팡이 Allomyces macrogynus의 홀씨와 팡이실에 존재하는 칼슘이온결합 단백질의 검색
박현숙,이수웅,오근희,박영식 인제대학교 1994 仁濟論叢 Vol.10 No.1
45Ca overlay를 이용한 방법으로 Allomyces macrogynus의 생장과정에서 형성되는 암수배우자 홀씨, 반수체 홀씨, 그리고 이배체 홀씨등의 세가지 홀씨와 팡이실에 존재하는 칼슘이온결합단백질들을 검색하였다. 세가지의 홀씨 각각에서는 모두 5가지 종류의 칼슘이온결합단백질들이 동일하게 관찰되었다. 팡이실에서는 적어도 6개 이상의 칼슘이온결합단백질들이 관찰되었으며, 이중에 3가지는 홀씨에서 관찰된 것과 동일한 것으로 판단된다. Ca2+-dependent phenyl-sepharose를 이용하여 팡이실에서 SDS-PAGE 겔 상에서 19kDa의 분자량을 가지는 칼슘이온결합단백질을 분리하였다. 이 단백질은 등전점이나 열에 대한 안정성, 칼슘이온에 의한 전기영동상에서의 움직임의 변화 등으로 판단하건 데 calmodulin으로 추정된다. 따라서 Allomyces macrogynus의 홀씨와 팡이실에는 calmodulin을 비롯하여 다수의 칼슘이온결합단백질들이 존재하며, 이들 중에는 홀씨, 또는 팡이실에서만 관찰되는 것들도 있었다. Calcium binding proteins(CaBPs) present in 3 different types of zoospores and in mycelium of Allomyces macrogynus were screened by using 45Ca overlay method. Five different kinds of CaBPs were equally observed in gametes, haploid spores, and diploid spores, respectively. Six kinds of CaBPs were observed in fractions of DEAE-cellulose chromatography of mycelial extract. Among them 3 CaBPs were shown to be identical with those detected in zoospore extract. 19-kDa CaBP was isolated from mycelial extract by Ca2+-dependent phenyl-sepharose chromatography. The 19-kDa mCaBP showed calmodulin-like properties as determined by molecular weight, isoelectric point, and Ca2+-dependent mobility shift on SDS-PAGE. It is shown that a variety of CaBPs including calmodulin-like protein are present in zoospores and mycelium of Allomyces macrogynus and some are confined to either.
( Jin Woong Song ),( Jung Won Lee ),( So Hee Kim ),( Won Kun Oh ),( Sook Kyoung Cho ) 한국과학교육학회 2004 한국과학교육학회지 Vol.24 No.1
Students experience science not only through school science lessons but also through various other channels. Science-related facilities, including science museums and centers, are important channels for informal science education. Korea has a number of governmental and private facilities in which young people can experience various aspects of scientific activities, and many of them also provide the programs supporting out-of-school science activities. However, there has been no systematic survey study on those facilities providing out-of-school activities, thus they have not been used effectively as well as their social utility is not fully realized. To this end, in 2002 researchers of this study carried out surveys (1) of the facilities supporting out-of-school science activities and (2) of science programs provided by these facilities. The surveys show that there are approximately 180 facilities supporting science activities in Korea. More than 40% of them are located in Seoul and Gyeonggi areas. Among them, the proportion of special theme science museums was the greatest (37.9%). The facilities supporting out-of-school science activities usually do not target the specific age groups but are intended for all people. The proportion of governmental facilities exceeds that of private ones. 41.8% of the facilities examined in this study run their own science activity programs. Among the 10 categories of the programs, `science class` type programs were the most common. There were more programs for elementary and middle school students than preschoolers, high school students and adults. The contents of the programs were more related to astronomy and meteorology, the observation of living things and field trips. Despite their high practical potential, the special theme science museums were found to be lacking in relevant programs, which could fulfill their values for informal science education.
도계처리과정에 사용되는 물에서 분리한 세균군과 각 세균종에 대한 살균제의 효능에 대하여
김성일,오경록,안동수,안태근,고경원,이민웅 동국대학교 대학원 1987 大學院硏究論集-東國大學校 大學院 Vol.17 No.-
The highest number of bacterial cell was observed in chicken washed water compared with the natural and chilled water. Fifteen bacterial strains were islated from the chicken processing water. When they were identified by the Bergey's Mannual of determinative bacteriology, they were all identified as the family Enterobacteriaceae; one genus of Citrobacter, three genera of Enterobacter, and four genera of Klebsiella except one isolate unknown. The concentration of Na₂HCIO₄to inhibit all the bacterial strains 30ppm, on the other hand the concentration of CIO₂to retard bacterial growth was 70ppm. The effective concentration of CIO₂was various according to bacterial species.