Adriamycin 백서자궁경부 기질내 교원섬유에 미치는 영향

김원규,백태경,송석근 漢陽大學校 環境科學硏究所 1993 環境科學論文集 Vol.14 No.-

Adriamycin은 Streptomyces peucetius var. caesius에서 추출한 항균성 항암제로서 세포내의 핵산과 결합하여 핵산 및 단백질의 합성을 억제하므로 종양세포의 증식을 억제하는 화학요법제이다. 그러나 adriamycin은 암환자에게 투여하면 종양세포 뿐 아니라 정상세포에도 손상을 초래하여 여러가지 부작용을 일으키므로 저자는 adriamycin을 백서에 투여하면 자궁경부내 섬유모세포의 단백질 합성이 억제되어 교원섬유의 양 및 분포에 변화가 야기될 것으로 사료되어 체중 200mg 내외의 Wistar계 자성 백서를 질도말법으로 발정주기를 확인하고 백서 체중 kg당 5mg의 adriamycin을 5일간 복강내에 투여하였으며, 희생시키기 직전 다시 발정주기를 확인한 후 경동맥사혈로 희생하고 개복하여 자궁경부를 적출하였다. 적출한 자궁경부는 두께 6㎛의 연속절편을 만들어 일반적인 자궁경부 조직의 변화를 관찰하기 위하여 hematoxylin 및 eosin 염색을 새행하였고 교원섬유의 관찰을 위하여 van Gieson 염색을 시행하여 다음과 같은 결과를 얻었다. 1. Adriamycin 투여군 백서 자궁경부의 상피세포층은 대조군 백서 자궁경부의 상피세포층에 비하여 위축되었다. 2. Adriamycin 투여군 백서 자궁경부 기질내 교원섬유의 van Gieson 염색에 대한 반응은 모든 발정주기에서 감소하였다. 3. Adriamycin 투여군 백서 자궁경부 기질내 교원섬유의 분포는 대조군 백서 자궁경부 기질내 교원섬유 분포의 치밀도에 비하여 감소하였다. 이상의 결과를 종합한 바 백서에 adriamycin을 투여하면 자궁경부의 상피세포 및 섬유모세포에 독성으로 작용하여 상피세포층이 위축되고 교원섬유의 van Gieson 염색반응 정도 및 분포의 치밀도가 감소하는 것으로 사료된다. It is well known that adriamycin, an anthracycline antibiotics isolated from Streptomyces peucetius var. caesius inhibits DNA and RNA synthesis and exhibits a significant antitumor activity against various tumors. Although adriamycin is very effective against tumor cell proliferation, it may also damage the normal cells of the body and develop the toxic effects during treatment of cancer patients. In this experiment, the authors studied the effect of adriamycin on the rat uterine cervix, observing the changes in content and distribution of collagen fibers in the uterine cervix of the rat. Fourty female Wistar strain rats, weighing about 200gm and exhibiting normal estrous cycle, were used as experimental animals. After confirmaiton of estrous cycle by mean of vaginal smear, the animals of experiment and control groups were injected intraperitoneally with 5mg/kg of adriamycin diluted in 0.2ml of distilled water and with 0.2ml of normal saline for 5 days, respectively. On the 5th day after administration of adriamycin and normal saline the expeimental animals were sacrificed by carotid exsanguination. The obtained cervix uteri was fixed in 10%. neutral buffered formalin solation(pH 7.2) and paraffin sections, 6㎛ in thickness, were made. Histological preparations were prepated by way of hematoxylin and eosin stain for the general changes of uterine cervix, and van Gieson's stain for the changes of collagen fibers in rat uterine cervix. The results obtained were as follows : 1. Epithelial cells in the adriamycin treated uterine cervix were atrophied. 2. Reaction of collagen to van Gison's stain in the adriamycin treated uterine cervix was decreased at all stages of estrous cycle. 3. The distribution of collagen in the adriamycin treated uterine cervix was less compact than the control uterine cervix. Consequently, it is suggested that the atrophy of epithelial layer, a decreased reaction to van Gieson's stain, and less compact distribution of collagen fibers may be resulted from cytotoxic effect of adriamycin on the eptihelial cells and fibroblasts in the rat uterine cervix.

Colchicine이 백서 경골의 근위골단연골판에 미치는 영향

김원규,백태경,김부만 漢陽大學校 環境科學硏究所 1993 環境科學論文集 Vol.14 No.-

Colchicum automnale에서 추출한 식물성 alkaloid인 colchicine은 uric acid riboside를 phosphorolysis시켜 뇨산을 생성하는 효소인 uric acid riboside phosphorylase의 활성을 억제하여 급성 통풍성 관절염을 유발하는 다형핵백혈구(polymorphonuclear leukocyte)의 뇨산결절의 탐식과 이동, 운동성 및 주회성을 방해하므로 급성 통풍성 관절염의 치료 및 예방에 사용되는 약제이다. 또한 colchicine은 세포분열 과정중 방추사의 형성을 억제하여 세포분열중기중지(metaphase arrest)를 유발하는 항세포분열기능을 갖고 있다. 저자는 항통풍 및 항세포분열제로 작용하는 colchicine을 성장기의 백서에 투여하면 백서의 근위경골 골단연골판에 존재한 연골세포의 분열이 억제되고, 연골세포에서 합성 및 분비되는 기질조성에 변화가 초래될 것으로 사료되어 colchicine 투여후 야기되는 백서의 근위경골 골단열골판내 연골세포의 형태학적 변화와 연골기질 조성의 변화를 조직학적, 조직화학적 방법으로 추구하기 위하여 본 실험을 시행하였다. 실험동물은 출생 제2주, 제4주 및 제8주 경과된 백서를 사용하였으며 colchicine 투여군에는 체중 kd당 1.0mg의 colchicine을 1일 1회씩 7일간 복강내 주사하였고 대조군의 실험동물에는 동량의 생리적식염수를 복강내 주사하여 마지막 투여 익일에 경동맥 사혈로 희생하고 경골의 근위 1/3을 절단하여 일반적인 조직구조의 변화를 관찰하기 위하여 hematoxylin 및 eosin염색을 시행하였고 경골의 근위골단연골판의 기질조성의 변화를 관찰하기 위하여 alcian blue(pH 2.5)-PAS 이중염색을 시행하고 광학현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. 출생 제2주의 colchincine 투여군 백서 경골의 골단연골판에서는 연골세포로 구성되는 세포주의 배열은 불규칙하고 증식대는 협소해졌으며, 골단연골판내 구기질은 alcian blue에 중등도의 양성반응을 나타내었고 구간기질은 alcian blue에 미약한 혹은 약한 양성반응을 나타내였다. 2. 출생 제4주의 colchincine 투여군 경골의 골단연골판에서는 증식대 및 비대대가 협소해졌고, 골단연골판내 구기질은 alcian blue에 중등도의 양성반응을 나타내었으며 구간기질은 alcian blue에 약한 양성반응 및 PAS에 중등도의 양성반응을 나타내었다. 3. 출생 제8주의 colchincine 투여군 백서 경골의 골단연골판에서는 증식대 및 비대대가 협소해졌고, 골단연골판내 구기질은 alcian blue에 중등도의 양성반응을 나타내었으며 구간기질은 alcian blue에 미약한 양성반응 및 PAS에 중등도 혹은 강한 양성반응을 나타내었다. 이상의 결과를 종합하면 성장기의 백서에 colchincine을 투여한 바, colchincine은 백서 경골의 근위골단연골판내 연골세포의 분열을 억제하며 산성점액질분비를 감소시키는 것으로 보아 골설장을 억제시키는 것으로 사료된다. Colchicine, and alkaloid obtained from the plant Colchicum automnale inhibits the activity of uric acid riboside phosphorylase which catalyzes the release of uric acid from uric acid riboside by phosphorolysis, and interferes with phogocytosis of urate crystal inducing acute gouty arthritis, mobilization, motility as well as chemotaxis of polymorpho-nuclear leukocytes, and it has also been used as antimitotic agent. Colchicine arrests in the meatphase during mitoisis, due to failure of spindle formation. In this regad, the author studied the effect of colchicine on tibial epiphyseal plates. Male rats, Sprague-Dawley strain, 2nd, 4th and 8th week after birth, were used as experimental animals. The experimental animals were sacrificed at 8th day after intraperitoneal administration of colchicine of 1.0mg/kg of body weight during 7 days. The specimen obtained from proximal third of tibia were stained with alcian blue(pH 2.5)-PAS and hematoxylin and eosin stain for light microscopic study. The results obtained were as follows : 1. In the proximal tibial epiphyseal plate of colchicine treated rats at 2nd week after birth, the arrangement of cellular column was irregular and the narrow proliferative zone was detected. The territorial matrix revealed moderate activtiy for alcian blue, and the interterritorial matrix showed trace of weak activity for alcian blue. 2. In the proximal tibial epiphyseal plate of colchicine treated rats at 4th week after birth, the area fo proliferative and hypertrophic nones were decreased, and the territorial matrix revealed moderate activity for alcian blue and the interterritorial matrix showed weak activity for alcian blue and moderate or strong activity for PAS. 3. In the proximal tibial epiphyseal plate of colchicine treated rats at 8th week after birth, the area of proliferative and hypertrophic zones were decreased, and the territorial matrix revealed moderate activity for alcian blue and the interterritorial matrix showed trace activity for alcian blue and moderate of strong activity for PAS. Consequently, it is suggested that colchincine inhibit the division of chondrocytes in proximal epiphyseal plate and decrease acid mucopolysaccharide content in matrix of epiphyseal plate, thus colchicine inhibit bone growth in rats.

排水計劃 模型에 의한 公州濟民川의 排水能力 檢討

盧載卿,安秉基,金泰喆 忠南大學校 地域開發硏究所 1992 地域開發論叢 Vol.4 No.-

Drainage planning model was developed to examine the capability of flood drawdown in existing detention facilities and also to plan drainage system. This model is composed of inflow, storage, and outflow com-ponents. SCS unit hydrograph is used to generate inflow. Stage-volume relationship is used to analyze the capacity of storage. Outflow is depen-dent of the storage of canal, the rate of pumping, and the geometry of outlet control structures. Modeling is based on trial and error method that computation continues until simulation outputs approach the capacity of storage of detention facilities. Model developed was applied to Jemin stream basin of 7.6㎢ in Gongju city. Jemin stream is confluent to Keum river and have a backwater phenomenon in time of flood in Keum river. Peak flow of Jemin stream on 100 year design rainfall was 154㎥/s from SCS hydrograph. Pumping capacity was 57.9㎥/s and detention basin capa-city was 475.000㎥ from tangent line method that cumulative storage line in tangent to the rate of pumping. Using this drainage model, pumping capacity was 105‰/s with canal closed because the place of constructing the detention basin around Jemin stream is insufficient. But, the analysis of backwater based on standard step method showed that Jemin stream was not overflowed in time of Keum river's flooding. Therefore, it is diagonosed that the present stage of drainage system in Je-min stream is able to drain off 100 year design inflow.

한국근대기 일본인 이주어촌내 가옥의 변용양상에 관한 연구 : 외나로도 축정 1ㆍ2구 일대의 1열형 가옥을 중심으로

강경남,박중신,김성중,김태영 대한건축학회 2005 대한건축학회논문집 Vol.21 No.2

This paper aims to clarify transformation of I-line type houses of Japanese migrant fishing vi11age in Oenaro-do Island. The basic type of I-line type house has rectangular spatial configuration simply composed of shop(entrance), room, and kitchen in plan, and eaves line & grid openings in facade. The existing openings are transformed into the aluminium 4 sliding door. In case of alterations and additions, the water closet and bath room alter the interior space of basic plan. The room, boiler, and storehouse are attached to I-line type house, and enlarged more them l-line-4-column in plan. In case of new-built constructions, the basic type of one-story building is same to 1-line-4-column type expanded from I-line type 3-column, the two stories houses with shop are mostly represented, and the other houses enlarge the lot and building scale. These new-built flat slab houses have balcony and bond beam considering eaves line of adjacent houses in facade.

퇴행성 요추 전방전위증의 수술적 치료

이준규,김경태 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

Degenerative spondylolisthesis is the forward slippage of one vertebra to another due to degenerative arthritic changes in the facet joint without isthmic defect. This do not respond to the routine conservative treatment. Regarding the surgical procedures, it is now generally accepted that decompression with or without fusion. Authors reviewed 15 cases of the degenerative spondylolishtesis who were surgically managed with decompression and posterior fusion with CD (Cotrel-Dubosset) instrumentation at department of orthopedic surgery, Chungnam National University Hospital from July 1991 to October 1993. The results were follows : 1. The cases were 2 males and 13 females having age from 38 to 61 years (average 49.3). Follow up duration was average 16 months. 2. The vertebral slippage was occurred at L3-4 2 cases, L4-5 8 cases, L5-S1 5 cases. 3. Slippage percentage was average 18.5% and instability was found at 9 cases(60%) 4. In preoperative myelogragm with computed tomography, there were stenosis and nerve root compression. In MRI, 15 cases were appeared degenerative change of intervertebral disc. 5. Reducibility was 60%(9 cases). 6. The pationts were rated according to the criteria by Gill etc.. Follow up results were exellent in 4 cases, good in 8 cases and fair in 3 cases. 7. In postoperative 6 months, there was no instability on plain flexion and extention lateral view. So we might conclude that surgical treatment with decompression and posterior fusion give good results to the degenerative spondylolishtesis.

Cadmium Chloride가 Mouse 의 췌장도 β-세포에 미치는 영향

전영희,백태경,정호삼,이규식,김영호 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

As the industrial use of cadmium has become more common, the question of its toxicity has become more important. The present was undertaken to study effects of cadmium on the structure of β-cells in the Langerhans islet of the mouse pancreas. Healthy mice of ICR strain weighing around 39gm were used in this experiment. The each animal of the experimental group was interperitoneally administrated cadmium chloride 5.0mg/kg diluted in the water for injection. The experimental animals were sacrified at 3, 6, 12, 24, 48 and 72 hours after administration of cadmium chloride and pancreatic tissues was excised. Some specimens were stained with Halmi stain to observe the morphological changes in the endocrine portion of the pancreas, the islet of Langerhan, and the others were prefixed in the 2.5% glutaraldehyde-2% paraformaldehyde phosphate buffer solution (pH7.0) and postfixed in the 1% osmic acid. Ultrathin sections were made and double strained with uranyl acetate and lead citrate. These preparations were observed with light microscope and electron microscope as well. The results obtained were as follow; 1. The number of β-cells throughout the pancreatic islets were markedly reduced in the 12 hours group and the β-cells were increased slowly in the 24 hours group. 2. The cristae of mitochondria disappeared and electron-density of matrix in mitochondria is decreased in the 3 hours after treatment of animals with cadmium chloride. The mitochomdria appeared almost like normal group, in the 12 hours group. 3. The cisternae of rough endoplasmic reticulum were dilatated in the 3hours group and change in rough endoplasmic reticulum were completely restored to normal in the 24 hours group. 4. The Golgi complexes were very hypertophied in the 3 hours. The Golgi complexes were observed like normal group, in the 12 hours group. 5. The sectetory granules were hypertrophied a halo and loss a dense core and the electron dense core and the electron dense of core in secretory granules decreased in the 3 hours, 12 hours group. But the secretory granules were slightly restored in the 48 hours group and appeared almost like normal group in the 72 hours group. Consequently, it is concluded that cadmium chloride exerts several toxic effects to β-cells of Langerhan's islet, especially, the damage to secretory granule in mouse.

혈당 측정용 스트립 개발에 관한 연구

권두한,정태화,남효진,송은영,변시명,김희정,김경아,이홍수 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1998 Journal of biomedical laboratory sciences Vol.4 No.2

과산화 효소와 포도당 산화효소의 효소반응을 이용하여 혈액 중 당을 측정하는 혈당측정용 스트립을 개발하였다. 멤브레인에 포도당 산화효소와 과산화 효소, 색원체를 건조 처리하면 혈당이 멤브레인에 처리된 포도당 산화효소와 즉각 반응하여 과산화 수소를 발생하고 발생된 과산화 수소가 과산화 효소와 반응하여 착색물을 형성한다. 제조된 스트립은 혈액과 접촉하면 반응시간 2∼3분 이내에 0∼800mg/dl의 혈당에 대하여 농도에 따라 연 녹, 청녹, 짙은 청색을 나타내며 이때 민감도는 40 mg/dl이었다. 육안용 혈당 스트립을 이용하여 정상을 포함한 당뇨 환자의 혈당 농도를 측정한 결과 Ames사와 BM사에서 시판하고 있는 제품과 유사한 결과를 얻을 수 있었다. 본 연구에서 개발된 스트립이 자동 분석기기의 재료로 활용될 수 있는지 여부를 알아보기 위하여 분광 비색계로 발색 반응의 최적 파장을 분석하고 최적 파장에서 각 혈당 농도별 반사 밀도를 측정하여 검정선을 얻었다. 이 검정선에 의해 임상 혈청시료의 혈당 농도를 측정한 결과 일본의 Kyoto Daiichi사의 혈액 분석시스템과 유사한 결과를 얻었다. 이로서 본 연구에서 개발한 혈당 스트립을 이용하여 혈액 중 혈당량을 육안으로 측정할 수 있음은 물론 자동 분석기기의 기본 시료로 이용될 수 있음을 확인할 수 있었다. We have developed a simple and accurate strip test that measures the blood glucose level semiquantitatively by visual observation, or qualitatively by using UltraScan spectrocolorimeter. The strip has solid phase reagents, including glucose oxidase, peroxidase, chromogen, affixed to a plastic support. The strip test is capable of measuring blood glucose level in the range of 0∼800 mg/dl and generating the results within 2 to 3 minutes. Human blood specimens obtained from normal individuals and the diabetic patients were evaluated by the new blood glucose strip and by the kit supplied by other commercial products. The test results exhibit the correlation coefficient of 0.964. The new test strip is proven simple and accurate, and it offers an alternative to the commercially available glucose tests.

저용량 Droperodol 의 지속적 정맥내 주사가 방사선 치료로 유발된 구토에 대한 효과 : As a Substitution for Granisetron for the Treatment of Gastrointestinal Symptoms from Palliative Radiation Therapy

문성록,박수경,정영표,김태요,최유선 圓光大學校 醫科學硏究所 1998 圓光醫科學 Vol.14 No.2

Background: Radiation therapy in palliative cancer management leads mainly to nausea, vomiting, dyspepsia and anorexia. Several agents have been used to treat above symptoms and sings. We assessed the usefulness of continuous intravenous infusion of low-dose droperidol, compared to granisetron for the control of gastrointestinal symptoms due to radiation therapy. Method: We devided 30 patients who developed gastrointestinal symptoms after undergoing palliative radiation therapy due to metastatic bone pain in two groups. In group G(n=15), the patients were injected granisetron(3㎎) and then injected it, 3㎎ per day. In group D(n=15), the patients were injected low-dose droperidol(0.25㎎) and then infused continuously at low-dose(1㎎/day) by the disposable continuous infusion pump. Result: There were no significant differences in the patient's satisfaction, improvement of appetite and incidences of side effects between two groups. Conclusion: The continuous intravenous infusion of low-dose droperidol can substitute for serotonin-receptor antagonist for the treatment of gastrointestinal symptoms during radiation therapy for cancer patients.

Cadmium Chloride가 흰쥐 신장에 미치는 영향에 관한 조직화학적 연구

이규식,백두진,김원규,정호삼,백태경,김영학 漢陽大學校 環境科學硏究所 1993 環境科學論文集 Vol.14 No.-

중공업의 발달에 따라 공장등지에서 중금속인 cadmium을 이용한 제품의 생산이 증가함에 따라 자연계내 토양, 물, 식물 등에 함유된 cadmium 뿐 아니라 공업제품과 공업 쓰레기에 함유된 cadmium은 쉽게 인체에 폭로되어 인체에 유해한 결과를 초래하게 된다고 알려져있다. 이에 저자는 cadmium이 생체내 신장에 특히 오랜기간 축적된다는 보고와 또 반감기가 긴 중금속이란 점에 착안하여 신장의 피질과 수질내 조직학적 변화를 관찰하고자 하였다. 실험동물은 사육실에서 순종 분리고 4년간 사육한 Wistar계 흰쥐를 사용하였다. 흰쥐에 체중 kg당 0.6mg의 cadmium chloride를 식염수 0.2ml에 용해하여 복강내로 10주간 매주 1회씩 투여한 후 희생기켜 신장조직을 paraffin 표본으로 제작하고 Mallory-Azan 염색을 시행하여 광학현미경으로 관찰한 후 다음과 같은 결과를 얻었다. 1. Cadmium chloride 10주간 투여후 1일 경과군에서 신장의 피질과 수질에 강한 Azan 염색반응을 보였으며 신사구체의 괴사와 수질 일부에서 간질성 섬유증식 및 울혈이 관찰되었다. 2. Cadmium chloride 10주간 투여후 1주 경과군에서는 신장의 피질과 수질에 중등도의 Azan 염색반응이 관찰되었고 신사구체의 국소적 괴사가 관찰되었다. 3. Cadmium chloride 10주간 투여후 2주 경과군에서는 신장의 피질에 중등도의 Azan 염색반응이 관찰되었으며, 4주 경과군에서는 피질과 수질에 약한 Azan 염색반응만 관찰되었다. 이상과 같은 결과로 미루어 cadmium chloride의 흰쥐에 장기간 투여는 미량이라도 신장의 신사구체의 괴사, 섬유화 등이 유발되나 투여후 4주 경과시는 손상이 회복되는 것으로 사료된다. The peritoneal injections of cadmium chloride(0.6mg/kg) per 1 week for 10 weeks were made. The cadmium chloride treated rats were sacrificed on 24 hours, 1 week, 2 weeks and 4 weeks after 10 weeks administration. And each of renal tissues from cadmium treated rat kidney was stained with Mallory Azan. 1. The severe glomerular necrosis, fibrosis and congestions in 1 day group were seen in the cadmium chloride treated rats medulla. 2. The moderately positive reaction for azan stain was seen in cortex and medulla of 1 week group in the cadmium chloride treated rats medulla. 3. The weakly positive reaction for azan stain was revealed in cortex and medulla of the rat kidenys on 2 weeks and 4 weeks after cadmium chloride administration for 10 weeks. It is concluded that the renal damage of rat resulted with administration of cadmium chloride may be recovered 4 weeks after injection.

Vincristine Sulfate가 백서 척수내 신경원의 Acetylcholinesterase 활성에 미치는 영향

이규식,이군자,정호삼,김승현,백태경 漢陽大學校 環境科學硏究所 1992 環境科學論文集 Vol.13 No.-

Vincristine은 협죽도과에 속하는 열대식물인 Madagascum periwinkle에서 추출한 천연 alkaloid계 항암제로서 암세포의 미소관 형성을 억제하고 미소관을 파괴하며 또한 핵산 및 단백질합성을 방해하여 암세포의 증식을 억제하므로 현재 백혈병 및 임파종등 혈액종양과 여러 충실성종양의 치료에 유용하게 사용된다. 그러나 다른 항암제와 마찬가지로 암세포에만 특이하게 작용하지 않고 정상세포에도 손상을 야기하므로 vincristinc을 투여받은 암환자에서 혈액독작용, 소화기장애, 피부장애 및 신경독작용등 여러 부작용이 유발되고 특히 신경독 작용은 vincristine을 사용을 제한하는 가장 심각한 장애로 알려져있다. 저자는 백서에 vincristine을 투여하면 vincristine이 실험동물의 척수내 신경원에 손상을 야기하여 척수내 신경원의 acetylcholinesterase활성에 변화가 초래될 것으로 사료되어 본 실험을 시도하였다. 실험동물로는 체중 200gm내외의 Wistar계의 웅성 백서 35마리를 사용하여 백서 체중 kg당 0.5mg의 vincristine sulfate(Abic Ltd.) 0.2cc를 1일 1회씩 5일간 복강내로 주사하였고 정상대조군 백서에는 동량의 생리적 식염수를 같은 방법으로 투여하였다. 실험동물은 최종 주사후 24시간 및 72시간 경과 후에 후두부 강타로 도살하고 10% neutral buffered formaline으로 관류시킨 후 육안으로 해부하여 척수를 절취하고 10% neutral buffered formaline에 4℃에서 18시간 고정후 일부 조직은 paraffin에 포매하여 Nissl체를 관찰하기 위한 cresyl violet염색을 시행하고, 일부조직은 -20℃로 조절된 cryostat로 두께 10㎛의 동결절편을 제작하여 Gredtzoff(1959)의 방법에 따라 acetyl-cholinesterase활성을 관찰하기 위한 조직화학적 처리를 한 후 광학현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. Vincristine투여군 백서의 척수내 체성운동신경원 및 내장성운동신경원에 chromatolysis가 유발되었으며, 소신경원의 수와 cresyl violet 에 대한 염색성이 감소되었다. 2. Vincristine투여군 백서 척수경부의 acetylcholinesterase활성은 1일 경과군의 복측각내 체성 운동신경원에서는 강한 양성반응을 나타내었고 배측각에서는 약한 양성반응을 나타내었으며 중간부에서는 미약한 양성반응을 나타내었으나 3일 경과군의 복측각내 체성운동신경원과 배측각내 교양질부에서는 중등도 양성반응을 나타내었으며 중간부에서는 미약한 양성반응을 나타내었다. 3. Vincristine투여군 백서 척수흉부의 acetylcholinesterase활성은 1일 경과군의 복측각내 체성운동신경원과 외측각내 내장성운동신경원에서는 약한 혹은 중등도 양성반응을 나타내었고 배측각에서는 약한 양성반응을 나타내었으며 척수수질의 중간부에서는 미약한 양성반응을 나타내었으나, 3일 경과군의 복측각내 체성운동신경원에서는 중등도 양성반응을 나타내었고 외측각내 내장성 운동신경원에서는 강한 양성반응을 나타내었으며 배측각내 교양질부에서는 중등도 양성반응을 나타내었으나 배측각의 나머지 부분에서는 약한 양성반응을 나타내었고 척수수질의 중간부에서는 미약한 혹은 약한 양성반응을 나타냈었다. 4. Vincristine 투여군 백서 척수요부의 acetylcholinesterase활성은 1일 경과군의 복측각내 체성운동신경원에서는 중등도 혹은 강한 양성반응을 나타내었고 배측각의 교양질부와 Lamina Ⅳ에서는 중등도 양성반응을 나타내었으며 배측각의 나머지 부분은 약한 양성반응을 나타내었고 척수수질의 중간부에서는 미약한 양성반응을 나타내었으나, 3일경과군의 복측각내 체성운동 신경원에서는 약한 혹은 중등도 양성반응을 나타내었고 배측각내 교양빌부와 Lamina Ⅳ에서는 약한 양성반응을 나타내었으며 척수수질의 중간부에서는 미약한 혹은 약한 양성반응을 나타내었다. 이상의 소견을 종합한 바, vincristine은 백서 척수내 신경원에 세포독으로 작용하여 신경원 손상을 초래하고 신경원의 acetylchlinesterase활성을 억제 하나, 이러한 손상은 시간이 경과함에 따라 일부 회복되는 것으로 사료된다. Vincristine, one vinca alkaloids, is widely used for chemotherapy of a variety of malignancy. The mechanism of anticancerous activity of this agent is based on inhibition of mitosis by interference with development of microtuble, of syntheses of nucleic acid and protein, and of destruction of cytoplasic membrane and microtubule. However, these inhibitory effects on cancer cell proliferation are non-specific, so clinical use of vincristine is frequently limited due to its toxic effects on the hematopoietic and nervous system especially. The author had undertaken this study to pursue the effect of vincristine on the nuerons in rat spinal cord histolgically and histochemically. Totals of 35 healthy male Wistar stain albino rats, weighing about 200 gm, were used as experimental animals. The experimental animals were divided into normal control and vincristine treated groups, and then vincristine treated rats were subdivided into 24 hours and 72hours groups by time passage. All vincristine treated rats were abministrated intraperitoreally with 0.5mg of vincristine per kg of rat body weight diluted in 0.2cc of normal saline for 5 days and rats of vincristine treated group were sacrificed by occipital blow at 24 hours and 72 hours after administration and then perfused by 10% neutral buffered formaline. The spedimen, spinal cord was collected and them fixed with 10% neutral buffered formaline during 18 hours at 4℃. For observing the histological change, cresyl violet stain was performed by routine pareffin method, and for histochemical study of acetylcholinesterase, frozen sedtions of 10 ㎛ in thickness were treated with Gerebtwoff method(1959). The results obtained were as follows : 1. In vincristine treated rat, a part of the somatic and visceral motor neurons were brought on chromatolysis. And the numer and the stain activities of small neurons were decreased in cresyl violet stain. 2. The activities of acetylcholinesterase of cervical segment in vincristine treated rat spinal cord were strongly positive at somatic motor meuron, weakly positive at dorsal horn and tracely positive at intermediate zone in 1 day passed group after the final administration, and moderately positive at somatic motor neuron and substantia gelatinosa, weakly positive at remainder of dorsal horn and tracely positive at intermediate zone in 3 days passed group. 3. The activities of acetylcholinesterase of thoracic segment in vincristine treated rat spinal cord weakly or moderately positive at somatic and visceral motor neurons, weakly positive at dorsal horn and tracely positive at intermdeiate zone in 1 day passed group after the final administration, and moderately positive at somatic motor mourons, strongly positive at visceral motor meurons, moderately positive in substantia gelatinosa, weakly positive at remainder of dorsal horn and tracely positive at intermediate zone in 3 days passed group. 4. The activities of acetylcholinesterase of lumbar segment in vincristine treated rat spinal cord were moderately or strong positive at somatic motor meurons, weakly positive at substantia gelatinosa and lamina Ⅳ, weakly positive at remainder of dorsal horn and tracely positive at intermediate zone in 1 day pssed group after the final administration, weakly or moderately positive at somatic motor neurons, weakly positive at sugstantia gelationsa and lamia Ⅳ and tracely or weakly positive at remainder of spinal medulla in 3 days passed group. It is consequently suggested that vincristine would induce the degeneration of neurons and inhibit acetylcholinesterase activity resulting from its neurotoxic effects on neurons in spinal cord of albino rats, but this damage would partially recover by time passage.