논에서 우분액비 시용이 총체보리 및 이탈리안 라이그라스의 생산성과 사료가치 및 환경오염에 미치는 영향

최기춘,나상필,정민웅,임영철,박형수,김종근,김원호,김맹중,최기준,김명화,이상락,김다혜,육완방,Choi, Ki-Choon,Na, Sang-Pil,Jung, Min-Woong,Lim, Young-Chul,Park, Hyung-Su,Kim, Jong-Geun,Kim, Won-Ho,Kim, Maeng-Jung,Choi, Gi-Jun,Kim, Myeong-Hwa,L 한국축산환경학회 2012 축산시설환경학회지 Vol.18 No.2

본 연구는 논에서 조사료 생산을 위한 작부형태별 우분액비의 시용효과를 규명하기 위하여 총체 벼 재배 후 우분액비 시용에 따른 총체보리 및 IRG의 생산성, 사료가치, 토양성분 및 용탈수중의 무기물 농도를 조사하였다. 본 연구는 2006년 5월부터 2009년 4월까지 3년 동안 전라북도 김제군 백산면 시험포장에서 완전임의배치 3반복으로 수행되었다. 총체보리의 2년간 평균수량은 7,520 kg/ha, 그리고 IRG는 평균 10,320 kg/ha으로 IRG가 총체보리 보다 현저하게 높은 수량을 보였다 (p<0.05). 이탈리안 라이그라스는 1번초가 2번초 보다 약 2배 이상의 수량을 보였다. IRG의 조단백질 함량은 총체보리보다 높았고 NDF, ADF 및 TDN 함량은 작물간에 큰 차이는 보이지 않았다. 경작형태별 우분액비 시용에 따른 토양내 pH, $P_2O_5$, T-N, OM 농도는 시험전에 비해 시험종료 후에 현저하게 증가하는 경향을 나타냈다 (p<0.05). 경작형태별 우분액비 시용에 따른 토양내 Ca, Na, Mg 및 K 농도는 시험전에 비해 시험종료 후에 현저하게 증가하는 경향을 나타냈다 (P<0.05). 경작형태별 우분액비 시용에 따른 용탈수중 $NH_4$-N, $NO_3$-N, $PO_4$-P, Cl, Ca, K, Mg, Na의 농도는 경작형태에 따른 확실한 차이를 보여주지 않았다. 따라서 논에서 우분액비를 이용하여 동계작물을 재배할 경우에는 수량도 중요하지만 환경적인 부분도 고려하여 우분액비를 이용해야 한다. This study was conducted to investigate the effects of cattle slurry on productivity of whole crop barley and Italian ryegrass and environmental pollution in paddy land. Cropping systems used in this study were consisted of two designs, such as whole crop barley applied with cattle slurry (WCB) and Italian ryegrass applied with cattle slurry (IRG). The field experiments were conducted on the clay loam at Backsanmyun, Kimje, Chunlabukdo province in Korea for three years (May 2006 to Apr. 2009). This study was arranged in completely randomized design with three replicates. The yields of WCB and IRG were 7,520 kg/ha and 10,320 kg/ha, respectively. The yields of IRG significantly increased as compared with that of WCB (p<0.05). The yield of Italian ryegrass in 1st cutting time was about 2-fold higher than that of 2nd cutting time. The contents of crude protein of IRG were higher than that of WCB. However, the contents of NDF, ADF and TDN were no difference between WCB and IRG treatments. The pH, and contents of T-N, $P_2O_5$ and organic matter (OM) in soil samples collected at the end of the experiment increased as compared with those at the beginning of the experiment (p<0.05). After the end of experiment, the concentrations of CEC (Ca, Na, Mg and K) in soil samples collected at the end of the experiment were remarkably higher than those at the beginning of the experiment (p<0.05). The concentrations of $NH_4$-N, $NO_3$-N, $PO_4$-P, Cl, Ca, K, Mg and Na in leaching water in paddy land cultivated with WCB and IRG were no difference between WCB and IRG treatments.

녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

Angiotensin 전환효소 유전자 다형성과 양극성 장애

김경나,김종우,정주호,이기철,정홍경,임성빈 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.2

연구목적 : 감정조절에 Renin-Angiotensin System이 관여하는 것으로 보고되어 왔다. 그 근거로 우울증의 새로운 약물치료로 주목받고 있는 Substance P의 대사에 angiotensin 전환효소(angiotensin-converting enzyme, ACE)가 관여한다는 것과 고혈압 환자에서 angiotensin 전환효소 억제제(ACE inhibitor)를 사용했을 때 다행감이나 우울감을 초래한다는 것 등이 있다. 본 연구에서는 ACE의 유전자 다형성을 분석하여 양극성장애와 ACE와의 연관성을 알아보고자 하였다. 방 법 : 양극성장애로 진단된 환자군 82명과 대조군 135명을 대상으로 16번째 intron의 다형성 부위를 가진 시발체 쌍(primer pair)을 사용하며 중합효소 연쇄반응(Polymerase Chain Reaction, PCR)을 시행하여 490bp 산물(I allele)과 190bp 산물(D allele)을 관찰함으로써 ACE 유전자의 유전자형(genotype)의 발현율과 대립유전자(allele)의 빈도를 비교 분석하였다. 결 과 : 양극성장애 환자군과 대조군 사이에서 유전자형의 발현율과 대립유전자적 빈도는 유의한 차이가 없었다. 결 론 : 양극성장애 환자군과 ACE 유전자 다형성 사이에 유의한 관련성은 없었다. 이 결과는 ACE가 양극성 장애의 원인으로 주요한 역할을 한다는 것을 시사하지 못했다. Objectives : A possible participation of the Renin-Angiotensin Systern(RAS) in regulating of the mood has been suggested by reports as follows : the angiotensrn converting enzyme(AGE1 is involved jn the metabolism of the neuropeptide substance P impficated with novel strategies for the pharmacotherapy of depression and the use of ACE inhibitors in hypertensive patients has been associated with euphoric or depressive stales. The aim of the present study was to analyze the role of the ACE gene I/D polymorphism for bipolar disorder. Methods : We examined the frequency of a polymorphism characterized by the insertion or deletion of a 287-bp Alu repeat sequence in intron 16 of the angiotensin converting enzyme gene(located on chromosome 37q233 in groups of patients with bipolar disorder(n=82) compared to healthy control subjects[n=135). ACE genotype was determined by size-analysis of polymerase chain reaction products. Results : The ACE ID polymorphism did not show any difference in allelic frequencies and genotypic distributions between bipolar disorder patients and control subjects. Conclusions : No significant association was found with bipolar disorder and the polymorphism of ACE gene. This finding does not support that ACE I/D polymorphism is a significant risk factor for bipolar disorder.

헤모글로빈의 Microencapsulation에 의한 인공적혈구의 산소전달과 용혈도에 관한 연구

나도춘,김기범,김성종,정경락 전북대학교 공학연구원 ( 구 전북대학교 공업기술연구소 ) 2003 工學硏究 Vol.34 No.-

In this study, we tried to improve oxygen transfer rate and reduce pressure drop on intravascular lung assist device with microencapsulation of red blood cells, because microcapsulation of red cells reduce blood hemolysis. Hemoglobin was purified from outdated human red blood cells used. Phospholipid was purified from egg yolk. The hemosome was prepared by encapsulation of hemoglobin with phospholipid multilayer, using rotary vacuum evaporator. The function of hemosome and hemosome/blood mixed solution was tested by measuring oxygen dissociation curve using blood gas analyzer. Also we were measured about hemolysis of hemosome, hemosome/blood mixed solution and blood by absorptivity method. As a results, the oxygen dissociation curve of hemosome and hemosome/blood mixed solution showed the same figure as the curve of normal red blood cells. Also, the hemolysis of hemosome and hemosome/blood mixed solution was measured less than normal red blood cell. Therefore, the oxygen transfer rate showed high use of hemosome, and hemosome/blood mixed solution rather than normal blood. Because microen- capsulation of normal red blood cells prevent hemolysis of normal red blood cells.

혈관 내 폐 보조장치에서의 기체교환속도의 향상

김기범,나도춘,김성종,정경락 전북대학교 공학연구원 ( 구 전북대학교 공업기술연구소 ) 2003 工學硏究 Vol.34 No.-

We intended to develope an vibrating intravascular lung assist device(VIVLAD) in order to exchange of efficiently gas transfer of oxygen and carbon dioxide exchange requirements for end-stage acute respiratory distress syndrome (ARDS) patients. However, the clinical study, concluded that more gas exchange was needed for intravascular oxygenation to be clinically effective in ARDS treatment. In this study, we tried to enhance gas exchange on the VIVLAD by using microencapsulation of hemoglobin, as the microencapsulation of hemoglobin reduces blood hemolysis, and perfluorocarbon emulsion(PFC emulsion). As a result, it was shown that the oxygen transfer of hemosome and blood/hemosome mixed solution were higher than that of whole blood. Also, it showed that the carbon dioxide transfer of whole blood/PFC emulsion mixed solution was higher than that of others. Therefore, we determined that hemosome and PFC emulsion could increase oxygen transfer and carbon dioxide transfer, respectively.

진동에 의한 혈관 내 폐 보조장치에서의 기체전달의 향상

나도춘,김기범,김성종,정경락 전북대학교 공학연구원 ( 구 전북대학교 공업기술연구소 ) 2003 工學硏究 Vol.34 No.-

When the Microporous hollow fiber membrane(MHFM) was contacted with whole blood, the serum leakage(membrane fouling) was occurred in the MFHM. In general, the MHFM was in use at most intravascular lung assist device(IVALAD) which was often occurred the serum leakage, and these serum leakage were reduced the gas exchange. In this work, we tried to improve gas exchange on the IVALAD by using vibration technique. As a result, it was showed that the vibrating method of this module was very effective due to the reduction of serum leakage on MFHM. Therefore, this method was increased gas exchange, respectively.

Effect of Ti Addition on the Microstructure and High-Temperature Oxidation Property of AlCoCrFeNi High-Entropy Alloy

Gi‑Su Ham,Young‑Kyun Kim,Young Sang Na,Kee‑Ahn Lee 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.1

This study investigated the effect of Ti addition on microstructure and high-temperature oxidation property of AlCoCrFeNihigh-entropy alloy. Ti content was controlled at 0 at% and 1 at%. The two alloys were found to have BCC single phase, andthe average grain sizes of Ti0.0 and Ti1.0 were 47.3 μm and 49.7 μm, respectively, showing similarity. The EDS mappingof the inside of grains found that both alloys were characterized to be divided into Al-Ni element rich region and Cr–Feelement rich region. As a result of high-temperature oxidation test at 1100 °C, oxidation weight gains were measured atTi0.0: 0.75 mg/cm2 and Ti1.0: 0.17 mg/cm2, respectively. The results indicate that Ti addition largely improved high temperatureoxidation resistance of AlCoCrFeNi HEA. Ti1.0 alloy, in particular, showed remarkably more excellent 1100 °Chigh-temperature oxidation resistance than other previously reported major ones such as NiCrAl and FeCrAl. In the surfaceand cross-section observations after oxidation tests, both alloys were found to have Al2O3oxides mostly. While the Ti0.0material was observed to have Al2O3spallation macroscopically, the Ti1.0 alloy showed Al2O3spallation only in some localareas. In addition, a unique result was found in AlCoCrFeNiTix(x=0,1) alloy that BCC → FCC phase transformation was accelerated,and FCC phase layer was formed in the surficial area where Al element had been depleted due to high-temperatureoxidation. Moreover, as Ti was added, the thickness of FCC layer induced by high-temperature oxidation decreased. Basedon the results, it was also discussed on how to improve the high-temperature oxidation resistance of AlCoCrFeNiTixHEA.

고속가공시 다중센서를 이용한 가공상태 감시 기술

김전하,강명창,김정석,나승표,김기태 한국공작기계학회 2000 한국공작기계학회 추계학술대회논문집 Vol.2000 No.-

The high hardened materials that are remarkable in aspects of durability have been used for die and mold industry. As the high hardened materials are hard to machine, the high-speed machining is essential to manufacture these materials. Currently, in the general turning and milling, experiments to the tool wear monitoring have studied, but those have not applied in high-speed machining. In this study, the cutting mechanism was analysed by the cutting force according to cutting conditions, and the parameters to monitor the tool wear were selected from the tendency of the cutting force and acceleration according to cutting length in the high-speed machining of the high hardened materials(STD11).

잡견에서 Rapid Atrial Pacing에 의한 심방의 전기적 재현성과 이의 회복

남기병,김동운,차성일,이석재,홍종면,임승운,나기정,조명찬 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.6

한국해안으로부터 Purple, Non-Sulfur Photosynthetic Bacterium, Rhodobacter sp. EGH-24의 분리 및 특성

차미선,김기한,조순자,이나은,이정은,이재동,이상준,박재림 한국환경과학회 2003 한국환경과학회지 Vol.12 No.12

A species of facultative photo-organotrophic, purple, non-sulfur bacterium was isolated from the 47 point at west and south coast of Korea in September 2001. Separated 13 samples of changes with red color under 28~32 ℃, 3000 lux, anaerobe conditions for 7 days cultivated in basal medium. For pure isolation from 13 samples, we used agar-shake tube method (0.4 % agar) and separated 5 strains through 13-repetition test. EGH-24 and EGH-30 was identified as the same strain through the RAPD(Random Amplified Polymorphic DNA)-PCR of strain EGH-9, EGH-13, EGH-23, EGH-24, EGH-30. Four isolates cultivated in synthesis wastewater for wastewater biodegradation test. EGH-24 was selected with efficient wastwater treating strain. Based on the results obtained from morphology, nutrient requirements, major bacteriochlorophyll content, 16S-rDNA phylogenetic analysis, EGH-24 strain may be identified as a new strain of the genus Rhodobacter and named Rhodobacter sp. EGH-24.