아미노산 수액의 신속한 정맥내 주사 후 담낭 수축에 관한 연구

김미영,김영수,최원,장재남,김명식,김인한,신용운,권계숙,이돈행,오윤주,김형길,이재수,김범수,조현근 대한소화기학회 1999 대한소화기학회지 Vol.33 No.1

Background/Aims: Gallbladder (GB) sludges and/or gallstones frequently develop due to hypomotility of GB after long-term total parenteral nutrition, abdominal surgery, bone marrow transplantatation, AIDS infection, trauma. The purpose of this study is to promote GB contraction and emptying with the rapid intravenous infusion of aminoacids and then, to determine the most effective dosage and infusion rate. Methods: After infusion of aminoacids, the volume of GB was measured serially using ultrasonography before infusion and immediately (0 min), 15 min, 30 min, 45 min and 60 min after infusion. The subjects of this study were 28 healthy male volunteers aged from 23 to 26. For control group (n=4), 250 cc of normal saline was infused for 30 min. For group A (n=8), 250 cc of aminoacids solution was infused for 30 min (21.2 g, 0.7 g/min). For group B (n=8), 250 cc of aminoacids solution was infused for 10 min (21.2 g, 2.1 g/mn). For group C (n=8), 125 cc of aminoacids solution was infused for 5 min (10.6 g, 2.1 g/min). Results: The volume of GB was significantly decreased with the lapse of time in A, B, C groups and the most significant change occurred at 45 min after infusion (p=0.0001). These groups showed significant volume change com pared to control group (p=0.0029). At 15 min after infusion, significant GB contraction occurred in group B, C compared to control group (p=0.0030). Only B group showed significant GB contraction at 45 min after infusion (p=0.0041). Conclusions: It is concluded that the intermittent rapid intravenous minoacids infusion may be useful to prevent GB sludges in the high risk groups. (Kor J Gastroenterol 1999;33:90 - 96)

Effect of Soybean Meal and Soluble Starch on Biogenic Amine Production and Microbial Diversity Using In vitro Rumen Fermentation

Jeong, Chang-Dae,Mamuad, Lovelia L.,Kim, Seon-Ho,Choi, Yeon Jae,Soriano, Alvin P.,Cho, Kwang Keun,Jeon, Che-Ok,Lee, Sung Sil,Lee, Sang-Suk Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.1

This study was conducted to investigate the effect of soybean meal (SM) and soluble starch (SS) on biogenic amine production and microbial diversity using in vitro ruminal fermentation. Treatments comprised of incubation of 2 g of mixture (expressed as 10 parts) containing different ratios of SM to SS as: 0:0, 10:0, 7:3, 5:5, 3:7, or 0:10. In vitro ruminal fermentation parameters were determined at 0, 12, 24, and 48 h of incubation while the biogenic amine and microbial diversity were determined at 48 h of incubation. Treatment with highest proportion of SM had higher (p<0.05) gas production than those with higher proportions of SS. Samples with higher proportion of SS resulted in lower pH than those with higher proportion of SM after 48 h of incubation. The largest change in $NH_3$-N concentration from 0 to 48 h was observed on all SM while the smallest was observed on exclusive SS. Similarly, exclusive SS had the lowest $NH_3$-N concentration among all groups after 24 h of incubation. Increasing methane ($CH_4$) concentrations were observed with time, and $CH_4$ concentrations were higher (p<0.05) with greater proportions of SM than SS. Balanced proportion of SM and SS had the highest (p<0.05) total volatile fatty acid (TVFA) while propionate was found highest in higher proportion of SS. Moreover, biogenic amine (BA) was higher (p<0.05) in samples containing greater proportions of SM. Histamines, amine index and total amines were highest in exclusive SM followed in sequence mixtures with increasing proportion of SS (and lowered proportion of SM) at 48 h of incubation. Nine dominant bands were identified by denaturing gradient gel electrophoresis (DGGE) and their identity ranged from 87% to 100% which were mostly isolated from rumen and feces. Bands R2 (uncultured bacterium clone RB-5E1) and R4 (uncultured rumen bacterium clone L7A_C10) bands were found in samples with higher proportions of SM while R3 (uncultured Firmicutes bacterium clone NI_52), R7 (Selenomonas sp. MCB2), R8 (Selenomonas ruminantium gene) and R9 (Selenomonas ruminantium strain LongY6) were found in samples with higher proportions of SS. Different feed ratios affect rumen fermentation in terms of pH, $NH_3$-N, $CH_4$, BA, volatile fatty acid and other metabolite concentrations and microbial diversity. Balanced protein and carbohydrate ratios are needed for rumen fermentation.

T Lymphocytes Negatively Regulate Lymph Node Lymphatic Vessel Formation

Kataru, Raghu P.,Kim, Honsoul,Jang, Cholsoon,Choi, Dong Kyu,Koh, Bong Ihn,Kim, Minah,Gollamudi, Sudheer,Kim, Yun-Keun,Lee, Seung-Hyo,Koh, Gou Young Elsevier 2011 Immunity Vol.34 No.1

<P><B>Summary</B></P><P>Lymph node lymphatic vessels (LNLVs) serve as a conduit to drain antigens from peripheral tissues to within the lymph nodes. LNLV density is known to be positively regulated by vascular endothelial growth factors secreted by B cells, macrophages, and dendritic cells (DCs). Here, we show that LNLV formation was negatively regulated by T cells. In both steady and inflammatory states, the density of LNLVs was increased in the absence of T cells but decreased when T cells were restored. Interferon-γ secretion by T cells suppressed lymphatic-specific genes in lymphatic endothelial cells and consequently caused marked reduction in LNLV formation. When T cells were depleted, recruitment of antigen-carrying DCs to LNs was augmented, reflecting a compensatory mechanism for antigen presentation to T cells through increased LNLVs. Thus, T cells maintain the homeostatic balance of LNLV density through a negative paracrine action of interferon-γ.</P> <P><B>Graphical Abstract</B></P><P><ce:figure id='dfig1'></ce:figure></P><P><B>Highlights</B></P><P>► Lymphatic vessels (LVs) are absent in the T cell zone of lymph nodes (LNs) ► T cells reduce LNLV density by secreting IFN-<B>γ</B> ► IFN-<B>γ</B> downregulates lymphatic-specific genes ► IFN-<B>γ</B> prevents lymphangiogenesis</P>

Phase II, multicentre, randomised trial of eribulin plus gemcitabine versus paclitaxel plus gemcitabine as first-line chemotherapy in patients with HER2-negative metastatic breast cancer

Park, Yeon Hee,Im, Seock-Ah,Kim, Sung-Bae,Sohn, Joo Hyuk,Lee, Keun Seok,Chae, Yee Soo,Lee, Ki Hyeong,Kim, Jee Hyun,Im, Young-Hyuck,Kim, Ji-Yeon,Kim, Tae-Yong,Lee, Kyung-Hun,Ahn, Jin-Hee,Kim, Gun Min,P Elsevier 2017 European journal of cancer Vol.86 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Paclitaxel plus gemcitabine (PG) combination chemotherapy is a preferred chemotherapeutic regimen for patients with metastatic breast cancer (MBC). Eribulin mesylate is a halichondrin non-taxane inhibitor of microtubule dynamics. A recent pooled analysis with eribulin showed improved overall survival (OS) in various MBC patient subgroups pretreated with anthracycline and taxane. Furthermore, eribulin may have less neurotoxicity than paclitaxel.</P> <P><B>Patients and methods</B></P> <P>This study was a prospective randomised phase II, open-label, two-arm, multicentre study comparing eribulin plus gemcitabine (EG) with PG chemotherapy as a first-line treatment for patients with human epidermal growth factor receptor 2-negative MBC. We hypothesised that EG chemotherapy would not be inferior to PG chemotherapy. The primary end-point was progression-free survival (PFS), which was estimated to be 70% at 6 months for each arm. The secondary end-points were as follows: OS, neuropathic scale, toxicity and clinical benefit rate.</P> <P><B>Results</B></P> <P>A total of 118 patients (median age: 50, 24–66) were enrolled between March 2015 and March 2016 and were randomly assigned to PG (<I>n</I> = <I>59</I>) or EG (<I>n</I> = <I>59</I>) chemotherapy. The mean number of metastatic sites was 3 (range 1–8). The 6-month PFS rates for both arms were 72% for EG and 73% for PG (<I>P</I> = <I>0.457</I>). There was no significant difference in OS between the two groups (not reached versus 21.2 months, <I>P</I> = <I>0.2234</I>). The median number of chemotherapy cycles for both groups was 10 for EG and 8 for PG (range 2–32). Clinical benefit rates were 44% for EG and 49% for PG. Major toxicities were neutropenia and neurotoxicity. Grade II or above neurotoxicity was more common with PG than with EG (13.6% for EG versus 45.8% for PG, <I>P</I> < <I>0.0001</I>).</P> <P><B>Conclusion</B></P> <P>EG chemotherapy had similar clinical benefits to PG chemotherapy in terms of PFS but less neurotoxicity.</P> <P><B>Trial registration</B></P> <P>KCSG BR13-11; ClinicalTrials.gov, NCT02263495.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This study was a prospective randomized phase II, multicentre study comparing EG with PG for MBC patients. </LI> <LI> The 6-month PFS rates for both arms were 72% for EG and 73% for PG (<I>P</I> = 0.457). </LI> <LI> EG chemotherapy had similar clinical benefits to PG chemotherapy in terms of progression-free survival but less neurotoxicity. </LI> </UL> </P>

Improved charge balance in phosphorescent organic light-emitting diodes by different ultraviolet ozone treatments on indium tin oxide

Kim, Dong Hyun,Lee, Won Ho,Jesuraj, P. Justin,Hafeez, Hassan,Lee, Jong Chan,Choi, Dae Keun,Song, Aeran,Chung, Kwun-Bum,Bae, Tae-Sung,Song, Myungkwan,Kim, Chang Su,Ryu, Seung Yoon Elsevier 2018 ORGANIC ELECTRONICS Vol.61 No.-

<P><B>Abstract</B></P> <P>Ultraviolet ozone (UVO) treatment of indium tin oxide (ITO) is a well-known technique to realize improved performance in organic light-emitting diodes (OLEDs). Herein, we report the comparison of UVO treatments on ITO at different energies (187 and 254 nm) in varying the charge balance in green phosphorescent OLEDs (Ph-OLEDs). The amounts and types of removed organic contaminants (such as -OH, C-C, C≡C, C=C, O=O, etc.) were observed to differ for each UVO energy. The 254-nm UVO treatment could not remove all the contaminants from the ITO surface, causing a decrease in hole injection (observed by hole-only device trends), due to the decrease in work function (WF) compared to the 187-nm-treated ITO. Moreover, the presence of large negatively charged O=O groups on the surface of 254-nm-treated ITO induced electronic repulsion (observed by electron-only device trends). However, remarkably, a suitable charge balance was attained by ITO treated with 254-nm UVO source over un-treated ITO, as well as with samples exposed to the higher-energy source (187 nm). The devices utilizing ITO treated with 254-nm UVO demonstrated the enhanced current efficiency of 67 cd/A, while the 187-nm UVO-treated and untreated ITO anodes exhibit those of 47 cd/A and 57 cd/A, respectively, at 5000 cd/m<SUP>2</SUP>. The variation in the WF of ITO with respect to different UVO sources and its role in the charge transport behaviors of the Ph-OLEDs were investigated in detail with contact angle measurements and interface barrier calculations through hole-only devices.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The effects of UVO treatment wavelength on ITO for Ph-OLED have investigated. </LI> <LI> Improved Charge balance achieved with 254 nm rather than 187 nm UVO treated ITO. </LI> <LI> Changes in work function and hole interface barrier in Ph-OLED have calculated. </LI> <LI> Superior EQE and current efficiency of 19.31% & 67.4 cd/A obtained with 254 nm UVO. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Harvesting near- and far-field plasmonic enhancements from large size gold nanoparticles for improved performance in organic bulk heterojunction solar cells

Shin, Jongmoon,Song, Myungkwan,Hafeez, Hassan,Jeusraj, P. Justin,Kim, Dong Hyun,Lee, Jong Chan,Lee, Won Ho,Choi, Dae Keun,Kim, Chul Hoon,Bae, Tae-Sung,Yu, Seung Min,Kim, Kyoung-Ho,Park, Hong-Gyu,Chung Elsevier 2019 ORGANIC ELECTRONICS Vol.66 No.-

<P><B>Abstract</B></P> <P>The high stability and strong coupling nature of gold nanoparticles (Au-NPs) than other metal counter parts have attracted the solar cell industry to pursue enhanced performances. Herein, we report on the improved performance of polymer bulk hetero-junction (BHJ) solar cells by the incorporation of large-size Au-NPs in the hole transport layer of poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS). To examine the reproducibility of the enhancement parameters, two different donor photoactive materials have been adapted and the role of larger-size (>70 nm, i.e. 71, 80, 87, 103 nm) Au-NPs in BHJ solar cells have been studied extensively. Significantly, when employing Au-NPs smaller than 80 nm, near-field coupling (localized surface plasmon resonance; LSPR) was prevalent, while the infusion of Au-NPs with sizes greater than 87 nm resulted in far-field scattering enhancement as the dominant effect, which was clearly determined using time resolved photo luminescence studies. The superior power conversion efficiency of 5.35% and 8.58% was achieved with PBDTTT-C: PC<SUB>61</SUB>BM and PTB7: PC<SUB>71</SUB>BM BHJs respectively, by employing 87 nm Au-NPs due to the balanced contribution of near- and far-field plasmonic effects, improved vertical coverage and better interfacial properties. This study illustrates that 87 nm Au-NPs is the maximum size to attain the improved efficiency, above which the rate of enhancement reduces dramatically.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Plasmonic enhancement in organic solar cells by gold nanoparticles (Au-NPs). </LI> <LI> Au-NPs with 71 and 80 nm shows dominant near-field enhancement. </LI> <LI> Au-NPs with 87 and 103 nm shows dominant far-field scattering enhancement. </LI> <LI> Improved vertical coverage and work function also reason for improved performances. </LI> <LI> 87 nm Au-NPs demonstrated the optimum condition of near- and far-field enhancements. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Enhanced device efficiency in organic light-emitting diodes by dual oxide buffer layer

Jesuraj, P. Justin,Hafeez, Hassan,Rhee, Sang Ho,Kim, Dong Hyun,Lee, Jong Chan,Lee, Won Ho,Choi, Dae Keun,Song, Aeran,Chung, Kwun-Bum,Song, Myungkwan,Kim, Chang Su,Ryu, Seung Yoon Elsevier 2018 ORGANIC ELECTRONICS Vol.56 No.-

<P><B>Abstract</B></P> <P>We have demonstrated an improvement in device performance of fluorescent organic light-emitting diodes (OLEDs) by inserting a dual anode buffer layer composed of tungsten oxide (WO<SUB>3</SUB>) and molybdenum oxide (MoO<SUB>3</SUB>). The advantage of adding dual anode buffer layers with different deposition sequences over individual and composite oxide buffer layers has been systematically analyzed based on their electronic and optical properties. The incorporation of single and composite buffer layers has been revealed to induce a very low injection barrier for holes in tri-layer fluorescent OLEDs which results in a charge imbalance in the emission layer. In contrast, a proper sequence of buffer layers (WO<SUB>3</SUB>/MoO<SUB>3</SUB>) exhibiting higher contact angle (lower surface energy) and higher surface roughness, together with a step-wise increment of potential barrier leads to a better overall charge balance in the active emission layer. Therefore, an enhanced current efficiency and power efficiency of ∼5.8 cd/A and ∼5.2 lm/W respectively were recorded for the WO<SUB>3</SUB>/MoO<SUB>3</SUB> buffer unit, which was better than the insertion of individual and composite layers.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The dual oxide buffer layers in fluorescent OLEDs have been explored. </LI> <LI> A dual buffer layer consists of tungsten oxide (WO<SUB>3</SUB>)/Molybdenum oxide (MoO<SUB>3</SUB>). </LI> <LI> The increment in hole interface barrier, higher contact angle and surface roughness of dual oxide layers is beneficial to achieve improved charge balance. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Improved stability of silver nanowire (AgNW) electrode for high temperature applications using selective photoresist passivation

Lee, Jong Chan,Min, Jeongho,Justin Jesuraj, P.,Hafeez, Hassan,Kim, Dong Hyun,Lee, Won Ho,Choi, Dae Keun,Cha, Jun Hwan,Lee, Chang Min,Song, Myungkwan,Kim, Chang Su,Ryu, Seung Yoon Elsevier 2019 MICROELECTRONIC ENGINEERING Vol.206 No.-

<P><B>Abstract</B></P> <P>Metal nanostructure arrays have been progressed as an alternative to the conventional oxides-based transparent conductive electrodes. Herein, we demonstrate the improved reliability of silver nanowire (AgNW) electrodes by photoresist encapsulation. The incorporation of photoresist followed by photolithography is beneficial to selectively pattern the AgNWs on poly[ether sulfone]. By varying the development or removal time of the ultraviolet (UV)-exposed photoresist, the properties of the AgNWs in the electrode are significantly varied. The optical parameters such as transmittance, haziness, and the yellow index of the electrodes have been extensively studied to reveal the advantage of the selective photoresist patterning. The AgNW electrodes patterned under 120 s of development time explored superior optical and electrical properties with high durability. The electrical properties of the AgNW electrodes at high temperatures (250 °C) demonstrate the photoresist-induced stability as compared to bare samples. Further, the morphological examination after the high temperature treatment reveals the reduced Rayleigh instability effects in 120 s developed AgNWs that facilitate the reliability under harsh conditions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Selective passivation of AgNWs electrode achieved with a photoresist. </LI> <LI> Haze/yellow index variation of AgNW electrode with various developing time revealed. </LI> <LI> AgNW passivated with 120 s developing time show high reliability until 250 °C. </LI> <LI> Selective passivation of AgNWs suppressed thermally induced Rayleigh instability. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Reactive oxygen species-dependent EndoG release mediates cisplatin-induced caspase-independent apoptosis in human head and neck squamous carcinoma cells

Kim, Jong Soo,Lee, Ji Hae,Jeong, Won Wook,Choi, Dae Hwa,Cha, Hee Jeong,Kim, Do Ha,Kwon, Joong Keun,Park, Soon Eun,Park, Jae Hoo,Cho, Hong Rae,Lee, Seon Ho,Park, Sang Kyu,Lee, Byung Ju,Min, Young Joo,P Wiley Subscription Services, Inc., A Wiley Company 2008 International journal of cancer: Journal internati Vol.122 No.3

<P>Cisplatin is a chemotherapeutic agent that is widely used to treat cancers such as head and neck squamous cell carcinoma (HNSCC). Previously, we have reported that cisplatin induced an early caspase-dependent apoptosis (8 hr) in a HNSCC cell, HN4. In this study, we examined a late caspase-independent apoptosis as well as an early caspase-dependent apoptosis in cisplatin-treated HN4 cells. While z-VAD-fmk, a pan-caspase inhibitor, blocked the caspase activities and protected cells from the early apoptosis, it did not provide protection against delayed apoptosis occurring after extended exposure (16 hr) to cisplatin, suggesting that the delayed apoptotic response in the presence of z-VAD-fmk was caspase-independent. Cisplatin treatment induced reactive oxygen species (ROS) generation, loss of the mitochondrial membrane potential (MMP) and nuclear translocation of endonuclease G (EndoG). Small interfering RNA mediated-knockdown of EndoG significantly protected cells from the delayed apoptosis induced by cisplatin in the presence of z-VAD-fmk. Overexpression of Bcl-2 in HN4 cells prevented loss of MMP, nuclear translocation of EndoG and protected cells from the delayed apoptosis induced by cisplatin in the presence of z-VAD-fmk. Pretreatment with N-acetyl-L-cysteine (NAC), a ROS scavenger, prevented both ROS generation, loss of the MMP and nuclear translocation of EndoG. Together, our data indicate that cisplatin treatment induced ROS-mediated loss of the MMP, and, then, the nuclear translocation of EndoG, which played a crucial role in caspase-independent apoptosis of HN4 cells in the presence of z-VAD-fmk. This is the first report about the involvement of EndoG in cisplatin-induced caspase-independent apoptosis of cells. © 2007 Wiley-Liss, Inc.</P>

Swiprosin-1 is expressed in mast cells and up-regulated through the protein kinase CβI/η pathway

Thylur, Ramesh P.,Kim, Young-Dae,Kwon, Min-Sung,Oh, Hyun-Mee,Kwon, Ho-Keun,Kim, Sang-Hyun,Im, Sin-Hyeog,Chun, Jang-Soo,Park, Zee-Yong,Jun, Chang-Duk Wiley Subscription Services, Inc., A Wiley Company 2009 Journal of cellular biochemistry Vol.108 No.3

<P>Swiprosin-1 exhibits the highest expression in CD8<SUP>+</SUP> T cells and immature B cells and has been thought to play a role in lymphocyte physiology. Here we report that swiprosin-1 is also expressed in mast cells and up-regulated in both in vitro cultured mast cells by phorbol ester and in vivo model tissues of passive cutaneous anaphylaxis and atopic dermatitis. Targeted inhibition of the specific protein kinase C (PKC) isotypes by siRNA revealed that PKC-βI/η are involved in the expression of swiprosin-1 in the human mast cell line HMC-1. In contrast, down-regulation of swiprosin-1 by A23187 or ionomycin suggests that calcium-signaling plays a negative role. The ectopic expression of swiprosin-1 augmented PMA/A23187-induced NF-κB promoter activity, and resulted in increased expression of cytokines. Moreover, knock-down of swiprosin-1 attenuated PMA/A23187-induced cytokine expression. Collectively, these results suggest that swiprosin-1 is a PKC-βI/η-inducible gene and it modulates mast cell activation through NF-κB-dependent pathway. J. Cell. Biochem. 108: 705–715, 2009. © 2009 Wiley-Liss, Inc.</P>