A Comparative Study on the Bioethics Consciousness of University Students on the Life-sustaining Treatment Decision Act

Junghyun Kim(Junghyun Kim),Dae-Eun Kim(Dae-Eun Kim),Eunseok Park(Eunseok Park),Dae-Jin Kim(Dae-Jin Kim),Hwanhee Kim(Hwanhee Kim) 한국보건의료윤리학회 2023 한국보건의료윤리학회지 Vol.2 No.1

Background: The purpose of this study is to provide basic data on the necessity and direction of education to establish a desirable bioethics view by identifying bioethics awareness of the Life-sustaining Treatment Decision Act among biomedical laboratory science student and nursing students attending K University. Methods: From October 30 to November 5, 2022, the final 279 people (144 in biomedical laboratory science and 135 in nursing) were analyzed through a structured survey of 29 questions. Results: As a result of comparing the presence or absence of learning and the degree of awareness of the life-sustaining treatment decision act, the presence or absence of learning p<.001. There was a statistically significant difference in cognitive degree p<.001. Conclusion: It is suggested that continuous research should be conducted to confirm the perception of biomedical ethics of Biomedical laboratory science students and nursing students and to find the need and direction of education to establish a desirable bioethics.

Subsequent platinum based re-treatment of platinum-resistant ovarian cancer: 7 cases review

( Junghyun Kim ),( Jina Yun ),( Ah Reum Chun ),( Se Hun Kim ),( Se Hyung Kim ),( Seong Kyu Park ),( Dae Sik Hong ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.1

Patients who relapse within 6 months after completion of therapy are thought to be “platinum-resistant"(Pt-R) and felt to have a worse prognosis. Currently, Single agent such as topotecan, liposomal doxorubicin, vinorelbine, docetaxel and gemcitabine is used as second line setting for patients with Pt-R ovarian cancer. But, some articles have been reported that patients who have Pt-R ovarian cancer may still benefit from re-treatment with platinum compounds after an interval of treatment with nonplatinum agents. The purpose of our study was to review our experience with subsequent platinum based re-treatment in women with Pt-R ovarian cancer. We studied seven patients who had relapsed within six months of their recent exposure to platinum. They were treated with platinum based combination with topotecan, irinotecan, or docetaxel. The median age was 52 years, six patients was received paclitaxel and carboplatin combination chemotherapy prior to re treatment with platinum compounds. They received a median number of six cycles as first line chemotherapy. Two patients achieved complete respons (CR) and 5 had stable disease(SD). The median time to progression(TTP) of 1st line treatment was 8.5 months (95% CI 7.8-9.3) and the median platinum free interval was 4.6 months. All of them had good performance status(ECOG 0) before 2nd line treatment. Four patients received docetaxel-carboplatin and 3 had topotecan/irinotecan-cisplatin combination regimen. A median number of 6 cycles as re treatment with platinum compounds was received. One patient achieved CR, one patient achieved partial response, while 5 patients achieved SD. The median TTP for these seven patients after re-treatment with platinum compounds was 7.3 months (95% CI 5.1-9.4). Four patients had progressive disease and received further salvage therapy with another regimen. The median overall survival from the time deemed to be Pt-R is 22.8 months (95% CI 18.8-26.8). Our small retrospective series suggest that the Pt-R category is still less clear. Patients who have been deemed Pt-R may still benefit from subsequent platinum based re-treatment.

Extract of Litsea japonica ameliorates blood-retinal barrier breakdown in db/db mice.

Kim, Junghyun,Kim, Chan-Sik,Lee, Ik Soo,Lee, Yun Mi,Sohn, Eunjin,Jo, Kyuhyung,Kim, Joo Hwan,Kim, Jin Sook Macmillan Press 2014 Endocrine Vol.46 No.3

<P>Loss of blood-retinal barrier (BRB) properties is an important feature in the pathology of diabetic retinopathy. Endothelium integrity is important for the normal vascular function. Litsea japonica (Thunb.) Jussieu is a Korean native plant that is consumed as a vegetable food. In this study, we evaluated the ability of an ethanol extract of L. japonica to prevent retinal vascular leakages in db/db mice, which is an animal model of type II diabetes. L. japonica extracts (LJE, 100 and 250 mg/kg) were administered once a day, orally, for 12 weeks. Vehicle-treated db/db mice exhibited hyperglycemia and retinal vascular leakage. LJE treatment blocked diabetes-induced BRB breakdown and decreased retinal VEGF expression in db/db mice. LJE also inhibited the degradation of occludin, which is an important tight junction protein. These findings support the potential therapeutic usefulness of L. japonica for retinal vascular permeability diseases.</P>

Lens epithelial cell apoptosis initiates diabetic cataractogenesis in the Zucker diabetic fatty rat

Kim, Junghyun,Kim, Chan-Sik,Sohn, Eunjin,Kim, Hyojun,Jeong, Il-Ha,Kim, Jin Sook Springer-Verlag 2010 Graefe's archive for clinical and experimental oph Vol.248 No.6

Scopoletin Inhibits Rat Aldose Reductase Activity and Cataractogenesis in Galactose-Fed Rats

Kim, Junghyun,Kim, Chan-Sik,Lee, Yun Mi,Sohn, Eunjin,Jo, Kyuhyung,Shin, So Dam,Kim, Jin Sook Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P>Cataracts are a major cause of human blindness. Aldose reductase (AR) is an important rate-limiting enzyme that contributes to cataract induction in diabetic patients. Scopoletin is the main bioactive constituent of flower buds from <I>Magnolia fargesii</I> and is known to inhibit AR activity. To assess scopoletin's ability to mitigate sugar cataract formation <I>in vivo</I>, we studied its effects in a rat model of dietary galactose-induced sugar cataracts. Galactose-fed rats were orally dosed with scopoletin (10 or 50 mg/kg body weight) once a day for 2 weeks. Administering scopoletin delayed the progression of the cataracts that were induced by dietary galactose. Scopoletin also prevented galactose-induced changes in lens morphology, such as lens fiber swelling and membrane rupture. Scopoletin's protective effect against sugar cataracts was mediated by inhibiting both AR activity and oxidative stress. These results suggest that scopoletin is a useful treatment for sugar cataracts.</P>

The Role of High-Mobility Group Box-1 Protein in the Development of Diabetic Nephropathy

Kim, Junghyun,Sohn, Eunjin,Kim, Chan-Sik,Jo, Kyuhyung,Kim, Jin Sook S. Karger AG 2011 American journal of nephrology Vol.33 No.6

<P>Abstract</P><P><I>Background/Aims:</I> The purpose of the experiment reported here was to assess the involvement of high-mobility group box-1 (HMGB1), receptor for advanced glycation end products (RAGE) and nuclear factor (NF)-κB signaling pathway in the development of rat diabetic nephropathy. <I>Methods:</I> Diabetes was induced by intraperitoneal streptozotocin injection in 7-week-old male rats. At 20 weeks of age, renal expression of HMGB1 was detected by immunohistochemistry. The expression of RAGE and NF-κB activity was studied by Western blot and electrophoretic mobility shift assay in renal tissues of normoglycemic and diabetic rats, respectively. <I>Results:</I> HMGB1 was highly expressed in both the cytoplasmic and nuclear patterns in diabetic renal glomerular cells and tubular epithelial cells, although in normal rats, HMGB1 was expressed only in the cell nuclei. The expression of RAGE, a potential receptor for HMGB1, and NF-κB activity were also greater in diabetic than in normal rats. Moreover, diabetes increased the binding of NF-κB to the RAGE promoter. <I>Conclusion:</I> These findings suggest that hyperglycemia-induced HMGB1 release may induce the renal injury in diabetic rats, and that the pathogenic role of HMGB1 might be dependent on RAGE and through activation of NF-κB.</P><P>Copyright © 2011 S. Karger AG, Basel</P>

Ethyl Pyruvate Prevents Methyglyoxal-Induced Retinal Vascular Injury in Rats

Kim, Junghyun,Lee, Yun Mi,Kim, Chan-Sik,Sohn, Eunjin,Jo, Kyuhyung,Shin, So Dam,Kim, Jin Sook Hindawi Publishing Corporation 2013 Journal of diabetes research Vol.2013 No.-

<P>Pyruvate is an endogenous antioxidant substance. The aim of this study was to investigate the protective effects of ethyl pyruvate (EP) on retinal vascular injury in diabetic retinopathy. To investigate the protective effect of EP on vascular cell apoptosis and blood-retinal barrier (BRB) breakage, we have used intravitreally methylglyoxal-(MGO-) injected rat eyes. Apoptosis of the retinal vascular cell that was stimulated by the intravitreal injection of MGO was evidently attenuated by the EP treatment. EP exerts inhibitory effect on MGO-induced vascular cell apoptosis by blocking oxidative injury. In addition, EP treatment prevented MGO-induced BRB breakage and the degradation of occludin, an important tight junction protein. These observations suggest that EP acts through an antioxidant mechanism to protect against oxidative stress-induced apoptosis in retinal vessels.</P>

Inhibitory Activities of <i>Cudrania tricuspidata</i> Leaves on Pancreatic Lipase <i>In Vitro</i> and Lipolysis <i>In Vivo</i>

Kim, Young Sook,Lee, Youngseop,Kim, Junghyun,Sohn, Eunjin,Kim, Chan Sik,Lee, Yun Mi,Jo, Kyuhyung,Shin, Sodam,Song, Yoojin,Kim, Joo Hwan,Kim, Jin Sook Hindawi Publishing Corporation 2012 Evidence-based Complementary and Alternative Medic Vol.2012 No.-

<P>To identify effective herb to treat obesity, we screened 115 herbal extracts for inhibition of porcine pancreatic lipase (triacylg-ycerol acylhydrolase, EC 3.1.1.3) activity <I>in vitro</I>. Of the extracts tested, <I>Cudrania tricuspidata</I> leaves exhibited the most pronounced inhibitory effect on lipase activity with an IC<SUB>50</SUB> value of 9.91 <I><I>μ</I></I>g/mL. Antilipid absorption effects of <I>C. tricuspidata</I> leaves were examined in rats after oral administration of lipid emulsions containing 50 or 250 mg <I>C. tricuspidata</I>/kg body weight. Plasma triacylglycerol levels 2 h after the oral administration of emulsions containing <I>C. tricuspidata</I> were significantly reduced compared to the untreated group (<I>P</I> < 0.05). These results suggest that <I>C. tricuspidata</I> leaves may be useful for the treatment of obesity.</P>

Cytoplasmic translocation of high-mobility group box-1 protein is induced by diabetes and high glucose in retinal pericytes

Kim, Junghyun,Kim, Chan-Sik,Sohn, Eunjin,Kim, Jin Sook SPANDIDOS PUBLICATIONS 2016 MOLECULAR MEDICINE REPORTS Vol. No.

<P>The aim of the present study was to assess the involvement of the high-mobility group box-1 (HMGB1) protein, receptor for advanced glycation end products (RAGE) and nuclear factor (NF)-κB signaling pathway in the development of diabetic retinopathy. Rat primary retinal pericytes were exposed to 25 mmol/l D-glucose for 48 h. Diabetic retinal vessels were prepared from streptozotocin-induced diabetic rats 12 weeks following the induction of diabetes. The expression of HMGB1 was detected using immunofluorescence staining. The expression of RAGE and the activity of NF-κB were analyzed using western blot and electrophoretic mobility shift assays, respectively. The results showed that HMGB1 was translocated to the cytoplasm of the high glucose-treated pericytes and diabetic retinal pericytes, whereas, in the control cells and the normal retinas, HMGB1 was expressed in the cell nuclei only. The expression of RAGE, a potential receptor for HMGB1, and the activity of NF-κB were also increased in the high glucose-treated pericytes, compared with the normal control cells. In addition, high glucose increased the binding of NF-κB to the RAGE promoter. These findings suggested that the cytoplasmic translocation of HMGB1 may be caused by diabetes and high glucose in retinal pericytes, and that the pathogenic role of HMGB1 may be dependent on the expression of RAGE and activation of NF-κB.</P>

The Difference on Chest CT Findings of COPD Patients Considering Regional Effect: Urban and Rural Area Near Cement Plant with Specific Dust Exposure

( Junghyun Kim ),( Woo Jin Kim ),( Bom Kim ),( So Hyeon Bak ),( Yeon-mok Oh ) 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.-

Purpose The clinical and radiological presentation of chronic obstructive pulmonary disease (COPD) has proposed heterogeneity according to the sources of inflammation characterized in COPD. This study tried to evaluate COPD phenotypes for specific dust exposure. Method This study was designed to compare characteristics and clinical outcomes with radiological findings between the two prospective COPD cohorts representing a distinguishing region in the Korea; COPD in Dusty Area (CODA) and the Korean Obstructive Lung Disease (KOLD) cohort. A total of 733 participants (n=186 for CODA, and n=547 for KOLD, for each) were included in the final analysis. Multivariate analysis for the comparison of lung function and CT measurements of both study groups after adjusting for age, gender, education, body mass index, smoking status, pack-year, charlson comorbity index, and the frequency of exacerbation were done entering the level of FEV1(%), biomass exposure and COPD medication into the model in stepwise. Result There was no differences in mean wall area (70.2±1.26 in CODA vs. 67.07±0.90 in KOLD, p=0.121). KOLD, where the COPD subjects from urban and metropolitan area, showed higher emphysema index (6.07±3.06 in CODA vs. 20.0±2.21 in KOLD, p<0.001, respectively). This significance in emphysema index was consistent even after further adjustment for FEV1 (6.12±2.88 in CODA vs. 17.3±2.10 in KOLD, p=0.002, respectively). Mean wall area was also found to be significantly lower in KOLD (70.2±1.21 in CODA vs. 66.8±0.88 in KOLD, p=0.028) after including FEV1 into the model. However, there was no difference in lung density between the two groups (p=0.077). Additional adjustment for biomass parameter and medication for COPD did not alter the statistical significance after entering into the analysis with COPD medication. Conclusion Higher mean wall area and lower emphysema index were observed in dust exposure region. These Results suggest that imaging phenotype of COPD is influenced by the environmental exposure.