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      • 넙치의 백화현상 규명에 관한 연구 : Ⅰ. 멜라닌 색소 생성에 미치는 효소와 기질의 영향 Ⅰ. Effects of Enzyme Activities and Substrates on Melanin Formation

        최영준,강석중,조창환,명정구,김종현 國立統營水産專門大學 附設 水産科學硏究所 1990 수산과학연구소보고 Vol.2 No.-

        멜라닌 색소의 생합성 과정에 관여하는 것으로 알려져 있는 효소 및 관련 인자와의 상관성을 검토하기 위하여 자연산 정상넙치와 양식산 백화넙치의 肉質과 表皮, 미립자 사료와 생물먹이인 Artemia 및 Rotifer에 대하여 단백질의 함량, 멜라닌 색소생성에 고나여하는 효소의 활성, 아미노산 조성 및 비타민A와 C의 함량을 비교하였다. 정상넙치表皮는 肉質에 비하여 단백질의 함량이 높았으나, 백화넙치인 경우는 대체로 비슷한 값이었다. 사료중의 단백질 함량은 미립자 사료 중 C1이 가장 높았다. catechal oxidase의 활성은 기질과 조효소 농도의 증가와 더불러 증가하였으며, 백화 유무에 관계없이 거의유사한 값으로 나타났다. 그리고 L-dopa oxidase활성도 동일한 결과였다. 정상넙치의 유리아미노산 함량은 백화개체에 비하여 높았으며, 表皮의 경우는 정상넙치가 백화너치에 비하여 7.5배 가량 높은 값을 나타내었다. 필수아미노산의 조성으로 비교했을 때, 미립자 사료는 Artemia와 Rotifer보다 우수한 단백질원이었다. 그리고 정상넙치의 함활아미노산의 함유량은 백화넙치의 6.3배에 달하였다. 비타민A는 정상 및 백화넙치의 肉質과 表皮에서 검출되지 않았으나 비타민C는 정상인 접치表皮가 백화넙치 表皮에 비해 약 7.8배였으며, 미립자 사료는 100.83mg/100ㅎ으로서 사료 중 가장 높았다. 따라서 넙치表皮의 멜라닌색소의 생성에는 기질인 방향족 아미노산과 함황아미노산이 큰 영향을 미치는 것으로 판단되었다. The albinic phenomenon of flat-fish (Paralichthys olivaceus) was investigated by measuring protein content, tyrosinase activity, amino acid composition, and contents of vitamin A and C. These materials in the flat-fish feed-stuff were also tested. The amount of skin protein was higher than that of muscle in normal flat-fish. Catechol and L-dopa oxidase activity did not differ between normal and albinic flat-fish. The free amino acid of skin in normal flat-fish was 7.5 times that in albinic one. Sulfur-containing amino acid in normal flat-fish was also 6.3 times that in albinic ones. Vitamin A was not detected in both of flat-fish. The content of vitamin C in normal flat-fish was 7.8 times that in albinic one. The contents of protein, sulfur-containing amino acid and Vitamin C in micro-encapsulated feed (one commercial feed in Japan) were the highest among the feed-stuff used in this experiment. The melanin formation of flat-fish skin was affected by substrates such as aromatic amino acid and cofactor such as sulfur amino acid.

      • 피부색을 이용한 얼굴 특징 성분의 실시간 검출

        최찬석,이정문 강원대학교 정보통신연구소 2004 정보통신논문지 Vol.8 No.-

        Face region detection is a very important step in the face recognition process for real-time applications. This paper presents a method to detect a face region using skin color information in Y·Cb·Cr color space. We propose a simple and highly applicable method of extracting facial features in the selected region. capturing a still image by a PC camera, we search a skin-like region using skin color filtering. Then we decide the face region by cropping the skin-like region. Finally, we extract each facial feature(eye, nose, mouth, and eyebrows) from the cropped region using histogram projection methods.

      • KCI등재

        상수도 배관의 부식감지시스템 개발 (Ⅰ)

        최윤석,김정구 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.10

        In order to develop a new corrosion sensor for detecting and monitoring the corrosion of buried pipeline, sensor element design and the correlation of its output to corrosion rate of steel pipe itself were evaluated by laboratory test methods in synthetic groundwater. It uses well-known principles of galvanic corrosion and consists of two dissimilar metals (anode and cathode) installed on the buried pipeline. In this paper, two types of electrochemical probes were used: galvanic cells containing pipeline steel (CS)-copper (Cu) and pipeline steel (CS)-type 304 stainless steel (SS) couples. The corrosion behavior in synthetic groundwater for the different electrodes was investigated by potentiodynamic test. The galvanic corrosion test was accomplished by a zero resistance ammeter technique. Weight loss measurements were conducted to obtain the corrosion rates of pipeline steel in synthetic groundwater. The correlation between galvanic current and corrosion rate was obtained by galvanic current measurement and corrosion rate measurements. The results of the potentiodynamic test indicated that copper exhibited an active corrosion behavior, while stainless steel demonstrated spontaneous passivation. In galvanic corrosion tests, the galvanic current of copper-steel couple was higher than that of other couple. The comparison of the sensor output and corrosion rate revealed that a linear relationship was found between the probe current and the corrosion rate. Especially, a better linear quantitative relationship was found between the Cu-CS probe current and the weight loss data of the pipeline steel coupons. In addition, the Cu-CS probe is more suitable for high resistance soil than SS-CS probe, due to the high current output.

      • KCI등재

        蔘茸扶正湯의 放射線 照射로 損傷된 組織 恢復 및 造血促進 效果

        崔昇勳,安圭錫,金正洙 대한동의병리학회 1997 동의생리병리학회지 Vol.11 No.2

        현재 서양의학에서 활용되고 있는 항암 요법으로는 外科處置, 化學療法, 放射線療法 및 免疫療法 等이 있으며, 그 중에서는 抗癌劑에 의한 화학요법과 방사선요법이 가장 많이 응용되고 있다. 그런데 이들은 癌細胞뿐만 아니라 正常細胞까지도 殺傷함으로써 腫瘍細胞를 억제하는 동시에 骨髓造血器, 消化系統 및 免疫機能을 포함하는 인체의 정상적인 기능을 손상시키는 문제점으로 그 사용에 제한을 받고 있다. 따라서, 본 연구는 蔘茸扶正湯의 항암요법으로 손상된 조직의 회복 및 조혈촉진 효과를 규명하기 위해 생쥐를 대상으로 하여 실험하였다. 그 결과, 생쥐 비장세포에 대한 增殖效果, 造血促進因子의 分泌能, 방사선에 대한 임파구의 防禦效果, 방사선에 대한 조혈세포 防禦效果, 방사선(9Gy)을 조사받은 생쥐의 生存率 등에서 유의성있는 결과를 얻어내어 아래와 같이 보고하는 바이다. Radiotherapy is an irreplaceable method of cancer treatment. But it has several side effects, especially damages to the hemopoietic and Immune system. Therefore radioprotectors are required to treat cancer successfully. A lot of Herbs and Herbal prescriptions are reported to have radioprotective effects. Above all, those to support the healthy energy and strengthen the body resistance are found more effective. This study was performed to evaluate the radioprotective effects of prescription Shenrong Fuzheng Tang(S. F. T), which consists of 16 kinds of herbs. We investigated proliferation of murine splenocytes, secretion of colony-stimulating-factors(CSFs), immunocompetence after irradiation in-vitro, and Endogenous spleen colony assay, survival assay in-vivo. When splenocytes were cultured with Shenrong Fuzheng Tang (S.F.T) (500㎍/㎖), proliferation was enhanced 5.7 times compared to control cultured with medium alone(P〈0.05) and, showed highest proliferation at 4th day after incubation. In order to evaluate stimulation of hemopoiesis of Shenrong Fuzheng Tang(S.F.T), the supernatant of splenocytes cultured with optimal concentration of Shenrong Fuzheng Tang(S.F.T) was used to measure CSFs secretion. The result showed enhanced secretion of colony-stimulating-factors(CSFs) compared to control(P〈0.05). To evaluate the protective effect of lymphocytes from irradiation, proliferation of splenocytes stimulated by LPS and ConA after incubation with Shenrong Fuzheng Tang(S.F.T) for 24h Prior to Irradiation(1∼3 Gy) was measured. The results showed higher proliferation of Shenrong Fuzheng Tang(S.F.T.) treated cells than that of non-treated cells. And percentage increases of irradiated splenocytes per non-irradiated splenocytes were also higher in Shenrong Fuzheng Tang(S. F. T)-treated cells than control. Endogenous spleen colony assay, to evaluate the protection of hemopoietic cells form irradiation, showed increased number of colonies(p=0.03) in Shenrong Fuzheng Tang(S.F.T) treated murine spleen(10.3±1.9) compared to non-treated murine spleen(3.4±0.8). Survival time of mice irradiated with lethal dose of γ-ray(9Gy) was prolonged in Shenrong Fuzheng Tang(S.F.T) treated group prior to irradiation as compared to non-treated group. According to these results we can suggest that prescription Shenrong Fuzheng Tang(S.F.T) has radioprotective effects and be used to protect the hemopoietic and immune system from damages of anti-cancer radiotherapy.

      • 휴대폰 컬러가 브랜드 개성, 브랜드 충성도 및 구매의도에 미치는 영향

        최정민;김준석 경희대학교 산업관계연구소 2008 産硏論叢 Vol.33 No.-

        본 연구는 브랜드를 효과적으로 차별화하고 지속적인 경쟁우위를 창출 할 수 있도록 휴대폰 컬러가 브랜드 개성, 브랜드 충성도가 구매의도에 어떠한 영향을 미치는가에 대해 검증하였다. 본 연구의 목적을 달성하기 위하여 현재 휴대폰을 사용하고 있는 서울시 20대-30대의 남성과 여성을 대상으로 조사를 실시하였다. 작성된 설문지는 총 350부 중에서 303부가 회수되어 분석에 부적합한 14부를 제외한 289부를 분석대상으로 하여 실증 분석을 실시하였다. 본 연구의 주요결과는 첫째, 휴대폰 컬러가 브랜드 개성에 영향을 미친다. 둘째, 휴대폰 컬러가 구매의도에 영향을 미친다. 셋째, 기업이 제품카테고리 속성으로서의 개성을 명확하게 전달하는 것이 충성도를 높일 수 있다는 것으로 나타났다. 넷째, 브랜드 개성요인에 따라 소비자의 구매의도에 영향력을 가지는 것으로 나타나 브랜드 충성도와 구매의도에 미치는 브랜드 개성요인이 일치 할 수 있음을 보여준다. 마지막으로 브랜드 충성도가 제품을 구매하고 주위사람들에게 브랜드의 제품을 추천하는 구전활동을 하면서 소비자의 구매행동과 밀접한 관련이 있게 된다. 본 연구는 향후 기업의 경영전략과 브랜드 구조에 적합한 컬러 프로그램의 체계적 수립과 전략적 관리는 뚜렷한 브랜드 개성을 가지게 해주며 기업 자산 가치의 성장에 막대한 영향을 끼치는 중요한 요소가 된다는 점, 이러한 브랜드 개성이 소비자들에게 브랜드 충성도를 가지게 하며, 구매의도에도 많은 영향을 미친다는 것에서 의의를 찾을 수 있다. This study aims to testify the effect of the color of a mobile phone on brand personality and of brand loyalty on a customer’s purchase intention were verified in this study so that mobile-phone brands could be effectively differentiated, the sales of mobile-phone companies would increase, and competitiveness would be fostered. The results of this study are: First, the color of a mobile phone influences brand personality. Second, the color of a mobile phone affects the customer’s purchase intention. Third, a company’s enhancement of its brand personality can boost the consumers’ brand loyalty. Forth, brand personality, which is the offshoot of the implementation of an effective product personality presentation and communication strategy, strongly influences purchase intention. Finally, brand loyalty influences purchase intention. Based on the results of this study, it can be concluded that companies should endeavor to strengthen their customers’ brand loyalty by utilizing brand communities, and should improve their performance. Moreover, the study results show that brand loyalty influences people to purchase a product and to relay information about it to others by word of mouth. In other words, brand loyalty was found to have a close correlation with the consumers’ purchase behavior. As this pertains to the actual purchase of a product, a company that endeavors to strengthen its customers’ brand loyalty can expect its sales to increase and can expect to become more competitive.

      • KCI등재

        SJO¨GREN'S SYNDROME 환자에게 즉시 임프란트 술식을 행한 치험례

        최정환,손동석,이지연,이윤서,안선희,이재목 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.5

        The old edentulous patients experience xerostomia commonly. They have considerable difficulty in wearing tissue-supported prosthesis and high risk rate of caries. One of them is Sj□gren's syndrome patient. Sj□gren's syndrome is a chronic inflammatory autoimmune disorder that results in a lymphocyte-mediated destruction of exocrine glands. We planned to place implants immediately after extraction in this case of autoimmune disease. The advantages of immediate implant placement include considerable decrease in time from tooth extraction to placement of the final prosthesis, fewer surgical procedures, and better acceptance of the overall treatment plan. We present a case of Sj□gren's syndrome patient treated with immediate implant placement & immediate loading in anterior region and immediate implant placement in posterior region.

      • SCOPUSKCI등재

        수평절골술에 의한 턱 성형술

        정영하,최기영,김석권 대한성형외과학회 1992 Archives of Plastic Surgery Vol.19 No.3

        Many methods are used for genioplasty, such as alloplastic implants, horizontal osteotomy, bone or cartilarge graft and so on. Among these methods, alloplastic implants and horizontal osteotomy are most frequently used. Augmentation of chin with a silastic rubber implant is the procedure of choice for mild form of microgenia. But this technique has disadvantages of possible malposition or bone erosion and is contraindicated in the patients with a moderate to severe degree of microgenia, which are apparent in both sagittal(anteroposterior) and horizontal dimensions. The preferred technique is the horizontal osteotomy, which yields excellent results, even in the difficult cases such as implant failure, asymmetry, or vertical mandibular disproportion. It is a safe, reliable, and simple procedure that is relatively free of either significant side effects or complications. We performed 6 cases of horizontal osteotomy and concluded as follows., 1. The procedure is relatively simple 2. This method is widely applicable to various conditions of chin deformity, and especially more natural degree of microgenia. 3. It effectively influences soft tissue in submental region as well as bone contour with a more natural aesthetic results. 4. The osteotomy line should be placed at least 2mm inferiorly to mental formen. 5. It is safe and relatively free of complications with maximal aesthetic results.

      • 간암의 발생과정에서 관찰되는 난원형 세포의 전자현미경적 연구

        최석렬,이중달 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

        Oval cells have been observed during chemical hepatocarcinogenesis in animals fed 2-acetylaminofluorene (2-AAF). Biological behavior of these cells remained poorly understood, and the hypothesis concerning about the origin and fate of these cells have been controversial. The author carried out the histologic and electron microscopic studies of oval cells in the livers from 80 Sprague-Dawley oval cells along with regenerating hepatocytes and bile ductular cells were subjected to the examination. Oval cells appeared about the periportal areas at the 10th day of the experiment on the histologic examination and at the 7th day on the electron microscopy. Individual oval cells at the earlier period and small groups of oval cells at the later period permeated into the perilobular areas making primitive ductular structures. The electron microscopic examination revealed oval cells having poorly developed organelles and plasma membrane of bile ductal cell type. The basal lamina invested partly the cytoplasm of oval cells. The ductular structure with microvilli were formed by oval cells in the later period of the experiment. The result suggested that oval cells were primitive cells to develop later into the bile ductular cells rather than hepatocytes.

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