HBx형질전환 생쥐에서 발생한 간세포암종에서 H-ras 및 c-myc의 발현에 관한 연구

문형배,소병준,김학철,윤기중,한원철,조향정,유대열,정영진 圓光大學校 醫科學硏究所 2002 圓光醫科學 Vol.17 No.2

<연구목적> HBx형질전환 생쥐에서 발생한 간세포암종의 발암과정에 종양유전자(H-rgs, c-myc)의 발현 정도를 조사하고자 하였다. <연구방법> 정상생쥐 12마리(4-18개월) 및 HBx 형질전환 생쥐 44마리(4-18개월)를 대상으로 포르말린에 고정하고 파라핀에 포매한 간 조직을 이용하여 면역조직화학적염색을 실시하였다. 실험군은 정상 부위, 이형성 부위 및 종양 부위로 구분하였으며, 종양 부위는 소결절성병변 부위와 간세 포암종 부위로 구분하였고, 이형성병변 부위는 이형성병변만 발견되는 부위, 소결절성병변과 동반된 이형성병변 부위 및 간세포암종과 동반된 이형성병변 부위로 구분하였다. <연구결과> H-rgs의 발현은 정상 간조직에 비하여 이형성병변 부위(P<0.05) 및 종양 부위(P<0.01)에서 증가하였으며, 소결절성병변 부위과 간세포암종 부위 사이에서는 간세포암종 부위에서 증가된 경향이었으나 통계학적으로 유의한 차이가 없었으며, 각 이형성병변 부위 사이에서도 유의한 차이는 없었다. c-myc의 발현은 정상 간조직 및 이형성병변 부위에 비해 종양 부위에서 증가하였으며(P<0.001), 소결절성병변 부위와 간세포암종 부위에서는 비슷하였고, 각 이형성병변부위 사이에서도 비슷하였다. <결론> HBx형질전환 생쥐에서 발생하는 간세포암종의 발생에 H-rgs는 이형성 변화를 일으키는 시기에 관여하며, c-myc은 이형성병변에서 암으로 이행하는 시기에 관여할 것으로 생각한다. Background: This experiment was designed for the expression of H-ras and c-myc in hepatocarcinogenesis of the HBx transgenic mice. Methods: Immunohistochemical stains in the paraffin embedded tissue of the liver were used for the detection of H-ras and c-myc in the 12 normal mice and 44 HBx transgenic mice of the 4-18 month old. Results: Expression of the H-ras was significantly increased in the dysplastic area (P<0.05) and tumor area (P<0.01) than in the normal liver. But there were no differences of H-ras expression between areas of microscopically identified hepatocellular carcinoma (MI-HCC) and grossly identified hepatocellular carcinoma (GI-HCC) and dysplastic areas among the only dysplastic areas, dysplastic areas with MI-HCC and GI-HCC. Expression of the c-myc was significantly increased in the tumor area (P<0.001) than in the normal liver and dysplastic area. But there were no differences of c-myc expression between areas of MI-HCC and GI-HCC, and dysplastic areas among only dysplastic areas, dysplastic areas with MI-HCC and GI-HCC. Conclusions: Our study suggests that H-ras is related to the dysplastic change and c-myc is related to the neoplastic change in the hepatocarcinogenesis of the HBx transgenic mice.

자율신경계의 활성도 측정을 위한 Data Acquisition System의 개발 및 임상응용

신동구,박종선,김영조,심봉섭,이상학,이준하 영남대학교 기초/임상의학연구소 2001 Journal of Yeungnam Medical Science Vol.18 No.1

Background: Power spectrum analysis method is a powerful noninvasive tool for quantifying autonomic nervous system activity. In this paper, we developed a data acquistion system for estimating the activity of the autonomic nervous system by the analysis of heart rate and respiratory rate variability using power spectrum analysis. Materials and methods: For the detection of QRS peak and measurement of respiratory rate from patient's ECG, we used low-pass filter and impedance method respectively. This system adopt an isolated power for patient's safety. In this system, two output signals can be obtained: R-R interval(heart rate) and respiration rate time series. Experimental ranges are 30∼240 BPM for ECG and 15∼80 BPM for respiration. Regults: The system can acquire two signals accurately both in the experimental test using simulator and in real clinical setting. Conclusion: The system developed in this paper is efficient for the acquisition of heart rate and respiration signals. This system will play a role in research area for improving our understanding of the pathophysiologic involvement of the autonomic nervous system in various disease states.

북송 이전과 이후 臟腑圖의 심장 형태요소의 비교

Hak Jun Jo 한국의사학회 2017 한국의사학회지 Vol.30 No.2

Purpose: This paper explores the extent to which ZangFu diagrams made after Nor thern Song Dynasty were influenced by ZangFu diagrams made before Northern Song Dynasty, and how diagram soft he whole body and heart or iginatedfrom MingTangZangFuTuand affected each other when quoted in books published after Northern Song Dynasty. Method: The author used four form factors including heart shape, internal curved lines, cylinder-like structures above heart, and wuzangxi(五臟系) which resemble salotus flower which has not yet bloomed, to analyze and compare different ZangFu diagrams before and after the Northern SongDynasty. Results: Two form factors, the shape of heart and wuzangxi, are describe dinZangFu diagrams before Northern Song, and may have influenced ZangFu diagrams after Northern Song. However, theot her two form factors (internal curved lines and cylinder-like structures above the heart) did not influence ZangFu diagrams after Northern Song. Allform factors of heart found in Wu Cang Liu Fu Zhi Tu (within the whole body) and diagram of heart in ZhenJiuJuYing(鍼灸聚英)were most similar, but the two paintings became less similaras they were cited in several books. With the exception of the form factor with structures above heart, the threeot her form factors are almost identical between two painting sproduced after Northern Song dynasty, as displayed in the same book. Conclusion:ZangFudiagramsoftheheartproducedafterNorthernSongDynasty,werenotcompletelybasedonZangFudiagrams made before Northern Song Dynasty. Diagrams of whole body and those of the heart were changed, while influencing each other after Northern Song.

기문침법(奇門鍼法)에 관한 연구(硏究)

조학준 ( Hak Jun Jo ) 경락경혈학회 2005 Korean Journal of Acupuncture Vol.22 No.4

Objectives and methods: This research aims to study about Gimunchimbeop(奇門鍼法) from original text of 『Singyeol(神訣) and Bosingyeol(保身訣)』. I could know is contents about Gimunchimbeop(奇門鍼法) through result that interprets original text of 『Singyeol(神訣) and Bosingyeol(保身訣)』 with Hyeonto(懸吐) and analyzes, and Gimunchimbeop(奇門鍼法)`s contents are as following. Results and Conclusions: 『Singyeol(神訣) and Bosingyeol(保身訣)』 is seen uses Honggukpoyeolbeop(洪局布列法)`s Saenggipalgwae(生氣八卦) among Gimunpogukbeop(奇門鍼法), and Yearngukpoyeolbeop(烟局布列法) that do Sigapoguk(時家布局) by putting first. It Introduced method that depends to Saenggipalgwae(生氣八卦) in Sigapoguk(時家布局)`s occasion and selects, and doctors depend to Bideungpalbeop(飛騰八法)`s Palmaekpalhyeolbaegugungpalgwaedo(八脈八穴配九宮八卦圖) deciding Bongwae(本卦) via a day to look at patient and selects Juhyeol (主穴), Assign Saenggipalgwae(生氣八卦) laying stress on Bongwae(本卦) and makes Bojohyeol(補助穴) equivalent to Saenggi(生氣), good luck(福德) and Cheoneui(天宜). but, this need study more should use distinguishing how because was not proved. Wonpando(圓板圖) and Babdo(方圖) from Eumyangdun(陰陽遁)`s from 1 Guk(局) to 9 Guk(局) are thing for Yearngukpoyeolbeop(烟局布列法). But it is few references about there decides Hyeolwi(穴位) that use even if arrange actually. Through 『Singyeol(神訣) and Bosingyeol(保身訣)』, I could know truth that took advantage of strange news to acupuncture methods(鍼法) but specific practical use method does not remain. Need to establish body of theory in reply in hereafter therefore and prove the effect through presence at a sickbed practical use.

“七方(칠방)”說(설)의 변화,발전 과정

조학준 ( Hak Jun Jo ) 대한한의학원전학회 2013 대한한의학원전학회지 Vol.26 No.4

Objective: This study is about how theory on seven kinds of prescriptions in Yellow Emperor`s Cannon of Internal Medicine(黃帝內經) had been developed and how it had been applied for in prescription books or clinical texts. Method: I made a comparison of this theory between prescription books and clinical texts. After it, I investigated the change or development of it. Result: The first explanation about this was made by Wang Bing(王氷). Yu Wanso(劉完素) made up several varieties and meanings of it, Jang Jahwa(張子和) corrected what Yu Wanso added. Besides, someone for example, Wang Hogo(王好古), Yi Cheon(李筵), and so on added new varieties and meanings of odd prescription and even prescription. Conclusion: Theory on seven kinds of prescriptions in Yellow Emperor`s Cannon of Internal Medicine had been constantly changed and developed in prescription books or clinical texts.

腦와 心의 한의학적 상관성에 대한 연구

조학준(Hak Jun Jo) 한의병리학회 2005 동의생리병리학회지 Vol.19 No.6

明代 醫學考試의 試卷인 『醫學統宗』의 「儒謂醫類小道其說當否」에 대한 분석

조학준(Jo Hak-jun) 대한한의학원전학회 2021 대한한의학원전학회지 Vol.34 No.4

Objectives : This paper analyzes the exam paper titled, ‘Is Traditional Chinese Medicine Just an Art?’ of the Yixuetongzong(醫学統宗) to provide basis for research on regional physicians’ examination in the Ming period. Methods : Cross examination, interpretation and content analysis of the exam paper were carried out. Results : This document is the examination paper that He Jian(何柬) submitted past the age of 40 at a regional examination for physicians that took place at the Duxue Wenyuan which is presumed to have been a regional education institute for medicine in Nanjing. While he had experience in anatomy, having been an army doctor when he was young, and was an experienced doctor who was an expert in acupuncture, it seems his goal to become a medical official at the royal medical center failed. He accumulated knowledge and experience as a Confucian doctor for more than 25 years under his teacher Pan Xiquan. It is unclear whether he had been educated at the regional medical education institute. Conclusion : The regional physicians’ examination during the Ming period promoted test takers to learn both medical and Confucian knowledge quite thoroughly.

정경혈(正經穴) 중(中) 맥동처(脈動處)와 혈위(穴位)의 동이(同異)에 대(對)한 연구(硏究)

조학준 ( Hak Jun Jo ),이평재 ( Pyeong Jae Lee ) 대한한의학원전학회 2007 대한한의학원전학회지 Vol.20 No.4

본초(本草)의 동미이용(同味異用)에 대(對)한 연구(硏究) -성무기(成無己)의 『주해상한론(注解傷寒論)』을 위주로-

조학준 ( Hak Jun Jo ),김호현 ( Ho Hyun Kim ),임강현 ( Kang Hyun Leem ) 대한한의학원전학회 2008 대한한의학원전학회지 Vol.21 No.1

『동의보감』 간장도의 특징에 대한 연구

조학준(Jo Hak-jun) 대한한의학원전학회 2022 대한한의학원전학회지 Vol.35 No.1

목적: 『동의보감』 간장도(肝臟圖)의 특징을 분석한다. 방법: 『동의보감』 간장도를 명당장부도(明堂臟腑圖)에서 기원한 장부분도(臟腑分圖)와 장부총도(臟腑總圖), 북송 이전의 장부총도, 도가(道家) 서적의 간장도 등과 비교하고, 그 특징을 『동의보감』에서 인용된 서적의 내용과 비교한다. 결과: 『동의보감』 간장도는 잎맥, 잎자루가 묘사되지 않은 점이 명당장부도 기원 또는 도가 서적의 그것과 매우 다르다. 『동의보감』 간장도는 간엽(肝葉) 8개가 아니라 간엽 7개와 줄기의 일부, 즉 절간(節間)에 해당하는 간장 본체 1개로 묘사된 것이다. 『동의보감』 간장도는 명당장부도 기원 또는 도가 서적의 간장도를 모사(模寫)한 것이 아니며, 『동의보감』에서 인용한 서적, 예를 들어 『난경』, 『소문』 왕빙(王冰) 주(注)와 『의학입문』 등의 기술에 따라 구체화한 것으로 추정한다. 결론: 간엽 7개, 간장 본체 1개로 묘사된 특징은 명당장부도 또는 도가 서적의 장부도를 모사하기보다 문헌 기술에 근거하여 변형된 결과로 추정한다. Objectives : The characteristics of the Liver diagram in the Donguibogam were analyzed. Methods : The Liver diagram in the Donguibogam was compared with diagrams originating from the Mingtangzangfutu(明堂臟腑圖), diagram from before the Northern Song period, Liver diagrams from Daoist texts, etc., after which its characteristics were examined in relation to quoted texts within the Donguibogam. Results : The Liver diagram of the Donguibogam is very different from that of the Mingtangzangfutu(明堂臟腑圖) or Daoist texts in that the leaf veins and leafstalk have not been depicted. The diagram in the Donguibogam depicts the liver as having seven lobes and one body instead of eight lobes. The Donguibogam diagram does not originate from the Mingtangzangfutu(明堂臟腑圖) nor is it an imitation of Daoist texts. It seems to be a visual realization based on descriptions from quoted texts such as the Nanjing, Yixuerumen, and Suwen annotations by Wangbing. Conclusions : The Liver with seven lobes and one body as depicted in the Donguibogam is likely a transformed version based on textual descriptions rather than an imitation of the Mingtangzangfutu(明堂臟腑圖) of images in Daoist texts.