HBx형질전환 생쥐에서 발생한 간세포암종에서 H-ras 및 c-myc의 발현에 관한 연구

문형배,소병준,김학철,윤기중,한원철,조향정,유대열,정영진 圓光大學校 醫科學硏究所 2002 圓光醫科學 Vol.17 No.2

<연구목적> HBx형질전환 생쥐에서 발생한 간세포암종의 발암과정에 종양유전자(H-rgs, c-myc)의 발현 정도를 조사하고자 하였다. <연구방법> 정상생쥐 12마리(4-18개월) 및 HBx 형질전환 생쥐 44마리(4-18개월)를 대상으로 포르말린에 고정하고 파라핀에 포매한 간 조직을 이용하여 면역조직화학적염색을 실시하였다. 실험군은 정상 부위, 이형성 부위 및 종양 부위로 구분하였으며, 종양 부위는 소결절성병변 부위와 간세 포암종 부위로 구분하였고, 이형성병변 부위는 이형성병변만 발견되는 부위, 소결절성병변과 동반된 이형성병변 부위 및 간세포암종과 동반된 이형성병변 부위로 구분하였다. <연구결과> H-rgs의 발현은 정상 간조직에 비하여 이형성병변 부위(P<0.05) 및 종양 부위(P<0.01)에서 증가하였으며, 소결절성병변 부위과 간세포암종 부위 사이에서는 간세포암종 부위에서 증가된 경향이었으나 통계학적으로 유의한 차이가 없었으며, 각 이형성병변 부위 사이에서도 유의한 차이는 없었다. c-myc의 발현은 정상 간조직 및 이형성병변 부위에 비해 종양 부위에서 증가하였으며(P<0.001), 소결절성병변 부위와 간세포암종 부위에서는 비슷하였고, 각 이형성병변부위 사이에서도 비슷하였다. <결론> HBx형질전환 생쥐에서 발생하는 간세포암종의 발생에 H-rgs는 이형성 변화를 일으키는 시기에 관여하며, c-myc은 이형성병변에서 암으로 이행하는 시기에 관여할 것으로 생각한다. Background: This experiment was designed for the expression of H-ras and c-myc in hepatocarcinogenesis of the HBx transgenic mice. Methods: Immunohistochemical stains in the paraffin embedded tissue of the liver were used for the detection of H-ras and c-myc in the 12 normal mice and 44 HBx transgenic mice of the 4-18 month old. Results: Expression of the H-ras was significantly increased in the dysplastic area (P<0.05) and tumor area (P<0.01) than in the normal liver. But there were no differences of H-ras expression between areas of microscopically identified hepatocellular carcinoma (MI-HCC) and grossly identified hepatocellular carcinoma (GI-HCC) and dysplastic areas among the only dysplastic areas, dysplastic areas with MI-HCC and GI-HCC. Expression of the c-myc was significantly increased in the tumor area (P<0.001) than in the normal liver and dysplastic area. But there were no differences of c-myc expression between areas of MI-HCC and GI-HCC, and dysplastic areas among only dysplastic areas, dysplastic areas with MI-HCC and GI-HCC. Conclusions: Our study suggests that H-ras is related to the dysplastic change and c-myc is related to the neoplastic change in the hepatocarcinogenesis of the HBx transgenic mice.

자율신경계의 활성도 측정을 위한 Data Acquisition System의 개발 및 임상응용

신동구,박종선,김영조,심봉섭,이상학,이준하 영남대학교 기초/임상의학연구소 2001 Yeungnam University Journal of Medicine Vol.18 No.1

Background: Power spectrum analysis method is a powerful noninvasive tool for quantifying autonomic nervous system activity. In this paper, we developed a data acquistion system for estimating the activity of the autonomic nervous system by the analysis of heart rate and respiratory rate variability using power spectrum analysis. Materials and methods: For the detection of QRS peak and measurement of respiratory rate from patient's ECG, we used low-pass filter and impedance method respectively. This system adopt an isolated power for patient's safety. In this system, two output signals can be obtained: R-R interval(heart rate) and respiration rate time series. Experimental ranges are 30∼240 BPM for ECG and 15∼80 BPM for respiration. Regults: The system can acquire two signals accurately both in the experimental test using simulator and in real clinical setting. Conclusion: The system developed in this paper is efficient for the acquisition of heart rate and respiration signals. This system will play a role in research area for improving our understanding of the pathophysiologic involvement of the autonomic nervous system in various disease states.

북송 이전과 이후 臟腑圖의 심장 형태요소의 비교

Hak Jun Jo 한국의사학회 2017 한국의사학회지 Vol.30 No.2

Purpose: This paper explores the extent to which ZangFu diagrams made after Nor thern Song Dynasty were influenced by ZangFu diagrams made before Northern Song Dynasty, and how diagram soft he whole body and heart or iginatedfrom MingTangZangFuTuand affected each other when quoted in books published after Northern Song Dynasty. Method: The author used four form factors including heart shape, internal curved lines, cylinder-like structures above heart, and wuzangxi(五臟系) which resemble salotus flower which has not yet bloomed, to analyze and compare different ZangFu diagrams before and after the Northern SongDynasty. Results: Two form factors, the shape of heart and wuzangxi, are describe dinZangFu diagrams before Northern Song, and may have influenced ZangFu diagrams after Northern Song. However, theot her two form factors (internal curved lines and cylinder-like structures above the heart) did not influence ZangFu diagrams after Northern Song. Allform factors of heart found in Wu Cang Liu Fu Zhi Tu (within the whole body) and diagram of heart in ZhenJiuJuYing(鍼灸聚英)were most similar, but the two paintings became less similaras they were cited in several books. With the exception of the form factor with structures above heart, the threeot her form factors are almost identical between two painting sproduced after Northern Song dynasty, as displayed in the same book. Conclusion:ZangFudiagramsoftheheartproducedafterNorthernSongDynasty,werenotcompletelybasedonZangFudiagrams made before Northern Song Dynasty. Diagrams of whole body and those of the heart were changed, while influencing each other after Northern Song.

『醫學入門·傷寒篇 』 類傷寒 病症 10종의 유래와 개념에 대한 연구

조학준(Jo Hak-jun) 대한한의학원전학회 2016 대한한의학원전학회지 Vol.29 No.4

Objectives : This paper seeks to study the provenances of 10 Symptoms of Para-cold damage from Yixuerumen, and discovers the rationality and originality of para-cold damages recognized by Li Chan. Methods : The paper looks through the books referenced by Yixuerumen to investigate the source text for Yixuerumen’s Para-cold Damage chapter, and the contents of the referenced books will be compared to that of Yixuerumen. Results : The sources of the 10 symptoms of para-cold damage from Yixuerumen Chapter of Cold Damage can be traced to Leizhenghuorenshu (expectoration, indigestion, rising temperature due to lack of energy, and beriberi), Wang Zhen’s Shanghanzhengzhimingtiao·Xinzengxuleishanghansizheng(the infection in a boil, blood sattis, caused overexertion, and pox), and Liu Chun’s Yujiweiyi’s chapter on Common Cold. Here, Li Chan made his own addition of internal damage and damage of overworking. Li Chan seems to have considered para-cold damage not as a type of cold damage but as a “concept relative to the broader range of cold damage.” In reflection of this understanding, Li Chan limited the number of categories to ten in accordance with the developmental trace leading from Leizhenghuorenshu to Shanghanzhengzhimingtiao. When we understand para-cold damage as a “concept relative to the narrower range of cold damage,” it could be used as a mean as for “Wenbin diagnosis,” but Yixuerumen’s para-cold damage is only utilized for the “diagnosis of a broader concept of cold damage.” Conclusions : Li Chan deserves credits for his academic rationality and originality displayed by the method he used to form para-cold damages where he used real cold damage and para-cold damage as principles, by the division of para-cold damage symptoms into ten provenances, by the creation of the concept of para-cold damage, and by his broad collection of prescription and treatment corresponding to each type.

논문(論文) : 본초(本草)의 동미이용(同味異用)에 대(對)한 연구(硏究) -성무기(成無己)의 『주해상한론(注解傷寒論)』을 위주로-

조학준 ( Hak Jun Jo ),김호현 ( Ho Hyun Kim ),임강현 ( Kang Hyun Leem ) 대한한의학원전학회 ( 구 대한원전의사학회 ) 2008 대한한의학원전학회지 Vol.21 No.1

일제강점기의 한의학 교재 중 하나인 『漢方醫學講習書』

조학준(Hak-jun Jo) 한국의사학회 2010 한국의사학회지 Vol.23 No.1

In July 2008, I obtained a copy of 『Hanbang Eihak Gangseupseo』 through Uibangseowon. It was recorded that the book was compiled by Seong Ju-bong and reviewed by Ji Seok-young. According to previous studies, this book was the lecture book that was used in teaching Traditional Korean Medicine in Daejeon, Chungcheongnam-do.. This book gave insight to the system and curriculum of the school for Traditional Korean Medicine in Daejeon, Chungcheongnam-do. It also exhibited the academic characteristics of Traditional Korean Medicine in the Period of Japanese Occupation and the medical viewpoint of Seong Ju-bong. The summary is as follows: First, an independent School for Traditional Korean Medicine was run in Daejeon, Chungcheongnam-do, with reasonable curriculums and systematic textbooks. Second, the medical viewpoint and treatment methods of Huang Yuan-Yu of Qing Dynasty was actively introduced. Then it was reorganized for the society and stimulated the progress of Traditional Korean Medicine. Third, while absorbing Chinese Medicine, it still inherited our heritage of Traditional Korean Medicine Especially, Seong Ju-bong’s original opinions and clinical experiences are shown in surgery, gynecology and pediatrics. Fourth, in a break from the past, when Chinese culture could not be introduced due to diplomatic problems between Joseon and Qing, efforts were made to overcome limitations of lagging behind by adopting and educating Warm Disease study. Fifth, while working side by side with Ji Seok-young who introduced the modern Western Medicine through vaccination, it still searched for a traditional Korean medical treatment for chickenpox. I hope that the report of my findings through reading 『Eihak Gangseupseo』 could make up for the fact that the medical history during the colonial period is scarce.

논문(論文) : 三焦(삼초) 形象圖(형상도)의 비교

조학준 ( Hak Jun Jo ) 대한한의학원전학회(구 대한원전의사학회) 2015 대한한의학원전학회지 Vol.28 No.3

Objectives : An atlas of tripple energizer affords to make us understand what the ancients thought it shaped. Methods : All pictures of it were picked out from traditional east Asian medical books where the atlas of every organ had been inserted. To be sure what it shaped, they were classified according to shape. Results : Shang Han Lun Tiao Bian(傷寒論條辨) has been one of the 4 books that shows it in the entire atlas drawing all organs in traditional Chinese, Korean and Japanese medical books. 14 books have a picture of it, while 18 books have an atlas of each organ except it. Since its multiple functions could not be expressed on a canvas at once, it had 6 different shapes, including the picture in Shang Han Lun Tiao Bian. Conclusions : Most of them were more representational or conceptualized than realistic, because ancient people had drawn it to inform the various functions, rather to show the actual feature. As anatomy went out into the east world, the more actually it was drawn, the further it was away from the own purpose.

宋ㆍ金ㆍ元 시대 광의의 三焦辨證

조학준(Hak Jun Jo) 한국의사학회 2015 한국의사학회지 Vol.28 No.1

Objectives : In order to prove the extended type of symptom discrimination for triple energizers in Song, Jin and Yuan Dynasty. Methods : Extract the topic-related contents from the books, with key words, triple energizers. After arraying them from this sources according to time order, analyze the data. Results : Analyzing its history from symptom, prescription, and discrimination proved the fact that the extended type of symptom discrimination for triple energizers had been developed continually, and assured that it did not play an important role in forming the narrow type of its symptom discrimination for Wen Bing (溫病) in Qing (淸) Dynasty, but also had been advanced separately from its type for Wen Bing. Conclusions : Symptom discrimination for triple energizers in Song, Jin and Yuan Dynasty gradually went far to become its extended type which widely used for Za Bing (雜病), Wen Bing, and diseases for pediatrics and dermatology.

『素問·血氣形志篇』의 背兪穴과 肝, 脾의 해부학적 위치의 상관성에 대한 연구

조학준(Jo Hak-jun),김동율(Kim Dong-ryul) 대한한의학원전학회 2017 대한한의학원전학회지 Vol.30 No.1

Objectives : This paper aims to shed light on the change in Korean medicine’s anatomical awareness through reviewing the locations and the interrelationship between back acupoints, live, and spleen as revealed in Suwen·Xueqixingzhipian. Methods : The locations of the back acupoints described in the Suwen·Xueqixingzhipian were compared with the information found in Neijing annotations, Lingsu, and other books on acupuncture, and the location of liver and spleen were compared with the figures of internal organs found in pre-Northern Song Period texts and with Neijing’s other chapters. Results : According to Taisu, the acupoint UB18 and UB20 as described in the back acupoints of Suwen·Xueqixingzhipian are located left side and right side of human body respectively, and this knowledge derives from an accurate anatomical awareness of the locations of liver and spleen. Although such anatomical awareness found in Taisu, which was published in the Sui Dynasty, was unable to influence the 10th century Oh Dynasty’s Yanluozi Neijingtu, it was gradually revised in Northern Song Period’s Qixifan Wuzangtu and Cunzhentu through autopsies. However, the Theory of Five Elements which described as seen in the other chapters in Neijing or Tang Dynasty Wang Bing’s annotation that liver is located at the left side of human body and spleen at the center exerted an immense influence over the subsequent generations’ future doctors. Even though Taisu disappeared in Korea and China at the end of Southern Song period, the accurate anatomical knowledge with regards to the locations of viscera and entrails were passed down through medical texts such as Ming Dynasty’s Yixuerumen.

『의학입문』 간엽(肝葉) 관련 문장의 새 해석

조학준(Jo Hak-jun) 대한한의학원전학회 2021 대한한의학원전학회지 Vol.34 No.1

Objectives : This paper aims to solve difficulties surrounding the verses related to lobes of liver in the Yixuerumen. Methods : First, relevant verses were selected from the Yixuerumen, then compared to texts from which they had originated. The differences and contrarieties between the texts were resolved through the perspective of plant taxonomy. Results : Wangbing’s ‘buye(布葉)’ was taken as the seed leaf of the dicotyledonous plant and corrected to ‘daye(大葉)’ by Li Chan. The expression ‘Yixiaoye(一小葉)’ was understood as describing the many main leaves that look like one big leaf, which grow in between the seed leaves. Nanjing’s interpretation of there being three leaves on the left, and four on the right describes the leaf pattern that grows in alternation. Conclusions : Through analysis of the verses related to lobes of liver in the Yixuerumen, we can presume that Li Chan had the knowledge and understanding required in plant taxonomy such as characteristics of a dicotyledonous plant and phyllotaxis.