파노라마 방사선사진에서 하악관과 이공의 관찰되는 정도

정성진,최의환,김재덕 대한구강악안면방사선학회 2001 Imaging Science in Dentistry Vol.31 No.3

Purpose : To determine the head position that the superior border of the mandibular canal as well as mental foramen can be more clearly visualized in panoramic radiography Materials and Methods : Ten dry mandibles were radiographed bilaterally using PM 2002 CC panoramic machine. A 20 mm thick aluminium filter was added to the slit collimator to obtain radiographs with acceptable density. The specimens were tilted by 2, 4, 6, 8, and 10 degrees downward with and without radiopaque markers. Radiopaque markers were inserted into the mandibular canals and the mental canals of each side of the specimens to serve as reference image when assessing the radiographs. The visibility of the mandibular canal and the mental foramen was estimated by 4 observers on all radiographs. The obtained results were analyzed statistically. Results : Mandibular canals were significantly more clearly visible in the radiographs with 4 and 6 degree downward position on both sides (P〈0.05). Mental foramens were significantly more clearly visible in the radiographs with 4, 6, and 8 degree downward on right side and 6 degree downward on left side (P〈0.05). There was not significant difference between right and left sides. Conclusion : Panoramic radiographs with 4 to 6 degree downward tilting could be valuable in locating the mandibular canal as well as the mental foramen. (Korean J Oral Maxillofac Radiol 2001; 31 : 153-8)

대장암종에서 암관련 유전자들의 Promoter Methylation과 암종 발생 및 진행과의 관게에 대한 연구

정동준,이정은,민영기,조성우,백무준,양승하,김의한,김창진 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

The colorectal corcinoma is one of the most common malignant tumor in Korea and its carcinogenic mechanism has not been elucidated exactly yet. So far, the most common carcinogenic mechanism has been known to be multiple genetic changes. But, the CpG island hypermethylation has been revealed to be one of the most important carcinogenic mechanism second to the genetic changes. The epigenetic changes characteristically imprint and transfer to the next generation without changes of nucleotide sequences. When the CpG island of promoter or 5'- exon 1 of a specific gene is methylated by DNA methyltrasferases (DNMTs), the gene expression is inhibited. Carcinogenic mechansisms by promoter methylation has been well known, but the progression of the cancer has not been elucidated yet. The molecular researches on the carcinogenic mechanisms largely depended on the tumor tissue containing various components of cell, due to difficulties in harvesting pure homogeneous tumor cells. Promoter methylatonof the cancer related genes as APc, hMLH2, p16, DAP-K and MGMT in 50 cases of sporadic colorectal carcinoma was studied. The pure normal colorectal epithelial cells, and cancer cells from superficial and deep invasive portion were harvested respectively by laser captured microdissection(LCM), and DNA was extracted from them. The promoter methylation was studied by methylation specific poymerase reaction (MSP) after bisulfite modification of the DNA and the expression of the genes by immunohistochemistry. The results suggests that promoter methylation of the cancer related genes are closely related with the colorectal carcinogenesis and it occurrs from early phase of carcinogenesis, progressing constantly to the late stage. And the promoter methylation of normal epithelial cells increased by age. The p15, known to be the genes related to hematologic malignancy, was closely related to the colorectal carcinoma as well.

鼻毛의 白毛發生에 의한 年齡推定

김진국,홍석찬,진정언,박의우,김동욱 大韓法醫學會 1999 대한법의학회지 Vol.23 No.1

The gray vibrissae of Korean males(n= 189) between 15 and 75 years old and females(n=111) between 15 and 74 years old whop visited the department of otorhinolaryngology, Konkuk university Chungju hospital during 1996 to 1998, were counted for the purpose of estimating approximate age range in individuals of unknown age. The lowest age appearing gray hairs in both sexes was 15-year-old. The Pearson's correlation coefficient in both sexes was 0.490 (p<0.01). The age appearing gray vibrissae in females was slightly earlier than that of males, however, the differences between right and left sides of nasal cavity were insignificant. This method of counting gray vibrissae would be useful to estimate age range in personal identification together with other methods available until now.

5-Fluorouracil 및 mitomycin C가 지라조직에 미치는 영향

고정식,안의태,박경호,김진국,김의한,정영신 대한췌담도연구회 2000 대한췌담도연구회지 Vol.5 No.2

인공 치아우식 발생 모델에서 디지털 방사선 공제술을 이용한 인접면 치아우식증의 진단

박정훈,최용석,황의환,이기자,최삼진,박영호,김경숙,진현석,홍경원,오범석,박헌국 대한구강악안면방사선학회 2009 Imaging Science in Dentistry Vol.39 No.1

Purpose : The purpose of the experiment was to evaluating the diagnostic ability of dental caries detection using digital subtraction in the artificial caries activity model. Materials and Methods : Digital radiographies of five teeth with 8 proximal surfaces were obtained by CCD sensor (Kodak RVG 6100 using a size #2). The digital radiographic images and subtraction images from artificial proximal caries were examined and interpreted. In this study, we proposed novel caries detection method which could diagnose the dental proximal caries from single digital radiographic image. Results : In artificial caries activity model, the range of lesional depth was 572-1,374 μm and the range of lesional area was 36.95-138.52mm². The lesional depth and the area were significantly increased with demineralization time (p<0.001). Furthermore, the proximal caries detection using digital subtraction radiography showed high detection rate compared to the proximal caries examination using simple digital radiograph. Conclusion : The results demonstrated that the digital subtraction radiography from single radiographic image of artificial caries was highly efficient in the detection of dental caries compared to the data from simple digital radiograph.

스쿠알렌 합성 유전자를 도입한 형질전환 가시오가피

서진욱,정재훈,최용의,이학성,신차균 중앙대학교 유전공학연구소 2003 遺傳工學硏究論集 Vol.16 No.1

The enzyme, squalene synthase, represents a branch point in the isoprenoid pathway capable of diverting carbon flow specifically to the biosynthesis of phytosterol and Triterpenoid. Transgenic Eleutherococcus senticosus plants were prepared by introducing the genes for squalene synthase derived from Panax ginseng (PgSS1), hygromycin phosphotransferase (HPT) and green fluorescent protein (GFP) through Agrobacterium-mediated transformation. Establishment of transgenic plantlets were confirmed by the presence of PgSS1 and HPT bands in the genomic DNA preparation using a PCR method and a Southern blotting. In addition, expression of the introduced GFP DNA was confirmed by observing green fluorescence of GFP from the embroyos. In the in vitro analysis of the PgSS1 enzymatic activities the transgenic plants showed to have 1.5 to 3 times higher than wild type plant, indicating that the PgSS1 genes was well over-expressed in the transgenic plants. Especially the PgSS1 acivities of embroyogenic cell was 120 times higher than those of plantlet.

장중첩증을 동반한 맹장 지방종 1예

홍의실,나병규,김민옥,유일영,채지영,채희복,윤세진,이상전,송형근,박길선,정현용 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.2

대장 지방종은 주로 우측 결장에 호발하고 특히 회맹판 부위와 맹장 부위에 호발한다. 또한 50~60대에 빈도가 높고 증상이 있는 환자에서 장중첩증이 높게는 40~50%에서 보고되고 있어 성인 장중첩증의 원인으로 중요한 비중을 차지한다. 특히 성인 장중첩증 및 장폐쇄의 중요 원인인 악성 종양과 임상 양상이 유사하여 감별진단이 중요하다. 저자들은 반복적인 복통을 주소로 내원한 환자에서 맹장의 4×5㎝의 점막하 지방종이 말단 회장부위와 반복적인 장중첩중이 발생했던 1례를 부분 절제술로 치료하여 양호한 임상 경과를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Lipomas are one of the most common benign nonepithelial tumors of the colon which are often detected incidentally by radiologic investigation or on operation. These tumors are usually without specific symptoms. Colonic lipomas occur most often in the right colon, particularly in the ileocecal valve and the cecum. Most patients are asymptomatic. but tumors larger than 2 cm tend to produce a change in bowel habit. abdominal pain. rectorrhagia and complications including luminal obstruction or intestinal intussusception. Diagnosis can be made by colonoscopy abdominal CT. MRI, or barium enema. We report a case of lipoma of the cecum that originated in the submucosa and presented as a intussusception in a 50-year-old woman with a brief review of the literature.

Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

19C 영국 청소년 문학 속에 나타난 남성다운 인간상의 스포츠

하남길,정의진 한국체육사학회 2003 체육사학회지 Vol.11 No.-

In the process of modernization, a great deal of British and American sports culture was diffused into out own educational system as well as our livelihood. However we lacked research in the areas such as the origin and background to the development of British sports culture. In a general point of view British Sports culture was a creation of several factors such as British politics, society, geography, religion and nationality. However in an ideological sense, the single most influential factor in the development of British sports culture was Muscular Christianity and at the nucleus of Muscular Christianity was the concept of manliness. Therefore this article was written with two main objectives. Firstly, 19th century juvenile literature and its ideals of masculinity were examined in order to reveal the figure of a manly juvenile. Secondly, this article examines the rearing of masculine juveniles and its correlation with the development of sports. In novels by Thomas Hughes such as BOP(The Boys Own Paper) the character of a masculine gentleman illustrated was similar to that of a Middle Age Knight with outstanding prowess in sports. This was the appearance of a typical gentleman of the late 19th century and among the characteristics demanded of them were; bravery, honesty, honour, patriotism, loyalty between friends, exceptional ability in masculine sports, empathy for the weak, an unyielding stance against the strong, manners and a worthy attitude of the defeated. That is to say, a courageous and loyal knight of the old. In order to create a template of a manly gentleman in upper class education, training of youths in organisations such as the Boyscout in addition to sports and outdoor activities were considered vital. These physical training bore the intent of prolonging the tradition of sturdy manliness. In addition, an ethical temperament was also emphasised. However, further emphasised than this ethical quality in juvenile literature of this age was to incite in youths the idol of a man; aggressive and harsh, brave, relentless and one that does not step back in the face of battle. To encourage these male temperaments, sports was considered important and as a result sports developed earlier in Great Britain than in other nations.

조경식물의 식재와 이용 : 원주시와 횡성군을 중심으로

원종화,정진형,김창섭,이기의 江原大學校 森林科學硏究所 2005 Journal of Forest Science Vol.21 No.-

본 연구에서는 강원도 원주시와 횡성군애에 식재 활용 되고 있는 조경 식물의 이용 상황을 조사 분석하여 현재 어떠한 식물이 어떻게 이용되고 있는가를 파악함으로서 그 이용방법에 대한 개선 방안을 모색하고 아울러 원주시와 횡성군의 아름다운 경관 형성을 위한 관리 방법 등을 제시하였다. This study was executed to find out how to improve the planting and use of landscaping plants in Weonju and Hoengseong. 1. The number of street trees were 22.068 and the species number were 10 species in Weonju in 2004. The major species of street trees were Ginkgo biloba(58%), Prunus sargentii(15%), Zelkova serrata(9%), Prunus armeniaca var, ansu(8%), and Acer palmatum(6%). The ratio of native species versus exotic were 50:50. In Hoengseong, the number of street trees was 13,500 and the specise number were 15 species. The major species of street trees were Prunus sargentii(42%), Ginkgo biloba(23%), Acer triflorum(12%) Prunus armeniaca var, ansu(6%), and Prunus mume(4%). The ratio of native species versus exotic were 67:33. The species of which planting frequency within two areas was very high were Ginkgo biloba and Prunus sargentii. 2. It is necessary to select tree species suitable for the characteristics of the locality and to raise distinctive street trees that contribute to the tourist industry. For the purpose, the appropriate street trees in two areas are Cornus controversa, Quercus aliena, Zelkova serrata. Prunus padus, Sorbus alnifolia, Sorbus comixta, Albizzia julibrissin, Acer triflorum. Styrax japonica, Chionanthus retususm Celtis sinensism, Prunus yedoensis, Malus sieboldii, Crataegus Pinnatifida, Prunus armeniaca var. ansu and Pyrus pyrifolia etc.. 3. Appropriate pruning adds to the aesthetic and prolongs the useful life, it also requires less managing of insects and diseases to maintain good healthy of street tees. Street trees were not properly pruned due to electric lines and shortage of pruning information. The pruning was controlled by Korea Electric Power Co, which has no pruning information. Pruning must be maintained by a professional landscape company to maintain good shape such as with Bonsai. The shrubs planting zone between street trees and other trees, and preservation plates were established for healthy of street trees. They have to be repaired and maintained well to keep better environmental conditions. The proper fertilization, the control of pests and diseases, the installation of drainpipe and the use of soil brought from another place were needed to improve the planting, use and maintenance of landscape plants. 4. The species number of school trees and flowers of 102 schools in Weonju and Hoengseong were 17species, 16species respectively. The major species of school trees in Weonju were Juniperus chinensis(24%), Ginkgo biloba(17%), Pinus densiflora(14%), Zelkova serrata(14%), and Pinus koraiensis(9&), and those of school trees in Hoengseong were Pinus koraiensis(44%), Abies holophylla(25%), Juniperus chinensis(8%), and Ginkgo binloba(8%). The major species of school flowers in Weonju were Rosa centifolia(47%), Forsythia koreana(24%), Magnolia kobus(12%), and Rhododendron schlippenbachii(6%), and those of school flowers in Hoengseong were Forsythia koreana(36%), Rhododendron schlippenbachii(33%), Magnolia kobus(6%) and Dicentra spectabilis(6%). 5. The species number of the protection trees designated by Woenju and Hoengseong were 15 species. The major species of protection trees were Zelkova serrata(100 trees), Ginkgo biloba(18) Pinus densiflora(7), Quercus spp.(5), Juniperus chinensis(4) and Alnus japonica(4). 6. The landscape plants planted around 2004 in weonju were Prunus yedoensis(2,563trees), Betula platyphylla var, japonica(2,000), Abies holophylla(1,785), Diospyros kaki(1,100), Prunus sargentii(880) and Prurus armeniaca var. arsu(708) etc.. The shrubs planted were Rhododendron obutusum(21,559 plants), Rosa certjfolia(7,150), Rhododendron yedoense var. poukhanense(5,950), Forsythia koreana(3,000) and Ligustrum obtusifolium(2,500) etc.. The landscape plants planted in Hoengseong Acer triflorum(928trees), Prunus yedoensis(455), Zelkova serrata(327), Thuja orientalis(261), Prunus sargentii(257), Pinus koraiensis(200), Prunus persica for, rubro-plena(200) and Pyrus pyrifolia(200) etc.. The shrubs planted were Rhododendron yedoense var, poukhanense(15,936). Syringa dilatata(10,090). Forsythia koreana(9,660). Cercis chinensis(3,200). Buuxus microphylla var, koreana(2,600) and Rosa centifolia(1,868) etc.. 7. The species numbers of the herbaceous planted in 2004 in Weonju were 24 species and the ratio of native species versus exotic were 7:17. The major species of perennial plants were Aster koraiensis(30,656 plants), Coreopsis drummondii(7,656), Rudbeckia bicolor(6,000), Chrysanthemum morifolium(4,850) and Chrysanthemum zawadskii var, latilobum(4,312). The major species of annuals and biennials were Cosmos bipinnatus(672,000plants), Zinnia elegans(35,600), Petunia hybrida(26,920), Viola tricolor(23,000), Helianthus annuus(17,000), and Geranium cinereum var, pubcaulescens(5,200). In Hoengseong, the numbers of herbaceous plants were 906,310 plants and the species numbers were 15 species. The major species of perennials plants were Aster koraiensis(70,480 plants), Hemerocallis fulva(20,070), and Phlox drummodii(18,000). The major species of annuals and biennials were Phlox hybrida(174,000 plants), Cosmos bipinnatus(125,000), Zinnia elegans(109,000), Tagetes patula(96,700), Vinca rosea(89,000) and Calendula officinalis(70,000). 8. Through these result, it was thought that the diversification of planting species, the selection of plants suitable to each space and the generalization of use of native species were needed.