분산 환경에서 RMI를 이용한 문제은행 시스템 설계 및 구현

김지연,정재열 신라대학교 교육과학연구소 2003 교육과학연구 Vol.- No.8

肝走査에 依한 肝 크기의 簡易評價

金支烈 全南大醫大 1977 전남의대학술지 Vol.14 No.2

姓娠中 血淸 3,3',5'-Triiodothyronine(Reverse T₃)의 變動

金支烈,李民化 全南大醫大 1980 전남의대학술지 Vol.17 No.2

카이토산을 이용한 방사성스트론튬 오염의 치료

김지열,송호천,양광희,최근희,채기문,범희승,김광윤 대한방사선 방어학회 1994 방사선방어학회지 Vol.19 No.3

본 연구에서는 마우스에 이미 오염된 방사성스트론튬(Sr-85)을 제거하는데 수용성카이토산이 어느정도 효과가 있는지 알아보고자 하였다. Sr-85를 정맥주사한 경우 주사후 1일째의 체내 잔류량은 72.9 ±5.7%, 5일째는 57.7 ±1.9%, 7일째는 54.2 ±1.4%로 서서히 감소하였으며, Sr-85를 복강내 주사한 경우는 주사후 5일째 54.4 ±1.2%, 15일째 50.6 ±0.8%로 정맥주사에 비해 낮은 잔류량을 보였다(5일째 잔류량의 비교, P<0.05), 0.3% 수용성카이토산을 1회 정맥주사해준 제21군 및 10% 수용성카이토산을 식이중에 섞어 먹인 제5군은 각각의 대조군에 비해 체내 잔류방사능의 차이가 없었으나 (P>0.05), 0.3% 수용성카이토산을 3일간 정맥주사한 제3군과 3% 수용성카이토산을 이틀 간격으로 15일간 복강내 주사한 제6군에서는 각각의 대조군에 비해 낮은 잔류방사능을 보였다. (P<0.01). 결론적으로 수용성카이토산을 연속적으로 정맥투여하거나 복강내 투여하는 경우에는 스트론튬의 골대사촉진 또는 골중의 스트론튬과의 반응등을 통해 그 배출을 촉진시킬 것으로 사료되었다. The aim of the present study was to elucidate the effect of the water soluble chitosans on the removal of contaminated radiostrontium(Sr-85) from the bone of mice. The remaining radioactivities in intravenously injected controls(group 1) were higher than in intraperitoneally injected controls (group 4, P<0.01). The % retention at day 5 were 57.7 ±1.9%, 54.4 ±1.2%, respectively. Single intravenous injection of 0.3% water soluble chitosan and continuous oral ingestion of 10% water soluble chitosan for 15 days were ineffective on the removal of contaminated radiostrontiums. Multiple intravenous or intraperitoneal injections of water soluble chitosan effectively removed contaminated radiostrontiums (P<0.01 vs controls). In conclusion, water soluble chitosan might remove once incorporated radiostrontium from bones of mice. further studies were needed to elucidate the mechanism of the removal.

血淸內 3,3',5'-Triiodothyronine (Reverse T₃)과 3,3',5 Triiodothyronine(T₃)의 動態에 關하여

金支烈,李民化 全南大醫大 1980 전남의대학술지 Vol.17 No.1

肝走査像에 依힌 硬變症 肝臟의 重量測定

金支烈 全南大醫大 1976 전남의대학술지 Vol.13 No.2

에탄올성 간손상 흰쥐의 유해산소 생성효소계에 미치는 칡 추출물의 영향

조수열,김명주,박은미,박지윤,장주연 한국노화학회 2001 한국노화학회지 Vol.11 No.1

임신마우스에서 수용성카이토산에 의한 태아의 방사성스트론튬 오염 억제

채기문,최근희,양광희,범희승,김지열,김광윤 대한방사선 방어학회 1994 방사선방어학회지 Vol.19 No.3

핵분열생성물중의 하나인 방사성스트론튬은 임신한 쥐에서 태반을 빠르게 통과하여 태아를 오염시킨다. 독성이 적은 천연착화제로서 마우스체내의 방사성스트론튬(Sr-85)의 제거에 효과적인 것으로 알려진 카이토산을 이용하여 Sr-85의 태반오염억제에 관한 연구를 하였다. 실험군은 일반식이를 공급한 대조군과 임신 17일째에 수용성카이토산을 피하(1% 카이토산), 구강(10% 카이토산), 복강(0.3% 카이토산)으로 주사한 카이토산군으로 분류하였다. 피하주사군은 Sr-85 오염후 카이토산을 주사한 군이며 복강과 구강주사군은 Sr-85 오염젼 15일간 카이토산을 공급한 군이었다. 출산과 동시에 어린마우스의 전신을 측정한 겨러과 출생후 7일째에 대조군이 2.8 ±0.3%의 전신축적율을 나타낸 반면 피하, 구강, 복강, 주사군은 각각 1.2 ±0.1%, 1.4 ±0.1%, 1.6 ±0.2%로 나타났다. 결과적으로 임신한 마우스에서 수용성카이토산은 방사성스트론튬의 태반오염을 유의하게 억제하는 것으로 사료된다. Radiostrontium passes the placental barrier in pregnant rodents very well. Chitosan, a natural nontoxic chelator, was reported to reduce whole body retention of radiostrontium in mice. The aim of the present study was to evaluate water soluble chitosan as a blocking agent of transplacental transfer of radiostrontium in pregnant mice. Twenty pregnant mice wre divided into four groups : control and three groups of chitosan treatment(groups 1 to 3). Sr-85(15KBq in 0.2ml saline) was subcutaneously injected into pregnant mice at the 17th day of pregnancy. In control mice, 0.2ml saline was given 5 hours after the injection of Sr-85. In group 1.1% water soluble chitosan was given subcutanously for two days, twice daily after the injection of Sr-85. In group 2, 10% water soluble chitosan was given orally for 15 days before conception. In group 3, 0.3% water soluble chitosan was injected intravenously for 15 days, once daily before conception. Gamma counting of newborns were done at days 0, 2 and 7 after their births. Whole body retention of Sr-85 in newborns of control mice at days 0, 2, 7 were 3.1 ±0.3%, 2.9 ±0.3%, 2.8 ±0.3% respectively. In experimental groups, whole body retention of Sr-85 was significantly lower thanthat of control(p<0.01) and no statistical difference was noted between them. In group 1, the values were 2.1 ±0.3%, 1.4 ±0.1%, 1.4 ±0.1%, respectively. In group 3, they were 2.1 ± 0.2%, 1.7 ± 0.2%, 1.6 ± 0.2%, respectively. In conclusion, the water soluble chitosan reduced transplacental contamination of radiostrontium in pregnant mice.

한국 정상인에서 연령에 따른 뇌혈류분포와 혈류예비능의 변화 : Tc-99m HMPAO SPECT 에 의한 연구

문대혁,이희경,송호천,이재태,범희승,손혜경,정환정,민정준,김지열 대한핵의학회 1999 핵의학 분자영상 Vol.33 No.3

Purpose: The aim of this study was to evaluate the normal values of regional cerebral blood flow (rCBF) and cerebrovascular reserve (CVR) in normal children to aged volunteers using Tc-99m HMPAO. Materials and Methods: Thirty four right-handed normal volunteers (20 males, 14 females, mean age 40.3±24.9 years, range 4 to 82 years) were underwent rest/acetazolamide (ACZ) brain SPECT using Tc-99m HMPAO and the sequential injection and subtraction method. rCBF was estimated on the basis of a semiquantitative approach by means of right/left ratio, region/cerebellum and region to whole brain ratios in frontal, parietal, temporal, and occipital lobes, basal ganglia, thalami, and cerebellum. CVR was measured by means of % perfusion increase calculated as % mean count change compared to rest rCBF in each regions. Results: Mean values of right to left ratios range from 1.004 to 1.018. rCBF was highest in cerebellum and lowest in basal ganglia and thalami. Frontal and temporal rCBF decreased while occipital and thalami rCBF increased according to age. No sexual difference of rCBF was noted. Mean CVR was 29.9±12.9%. Mean CVR significantly increased to late teens, and declined thereafter. After 6th decade, CVR in both frontal lobes, left parietal lobe and right basal ganglia decreased significantly with advancing age. There was no sexual difference of CVR. Conclusion: Quantitative assessment of CVR was possible by ACZ Tc-99m HMPAO brain SPECT. It revealed that rCBF and CVR changed according to age in normal Korean volunteers. There was no sexual difference.

Comparison of Genetic Toxicity Between Chitosan and EDTA in Mice

Kim, Kwang Yoon,Kim, Young Ho,Kim, Hee Kyung,Bom, Hee Seung,Kim, Ji Yeul,Lee, Eung Hoo 한국키틴키토산학회 1998 한국키틴키토산학회지 Vol.3 No.3

자연계에 풍부하게 존재하고 있는 키틴에서 제조한 키토산은 건강보조 식품 및 다양한 방면에서 사용되어지고 있다. 이 연구의 목적은 일반적으로 사용되어지고 있는 화학적 흡착제인 EDTA와 천연 착화제인 키토산의 독성을 비교하였다. 독성은 혈액 구성 인자와 염색체 이상 검사를 이용하여 세포유전학적 독성에 대해 비교하였다. 이를 위하여 숫컷 마우스에 EDT 수용성 키토산 및 불용성 키토산을 일반 먹이에 농도별로 달러 혼합한 후 7일동안 섭취하게 한 후 혈액 및 염색체내의 유전독성을 상호 비교하였다. 결과로서, EDTA 및 키토산을 처리하지 않은 대조군에 비하여 키토산을 처리한 실험군에서는 농도에 상관없이 혈액 및 유전독성은 피해를 나타내지 않아 천연 착화제인 키토산은 고농도로 섭취한 경우에서도 안전성이 있는 것으로 판정되었다. 그러나, 화학 착화제인 EDTA의 경우는 실험기간동안 유전독성 및 혈액 구성치의 전반적으로 독성을 나타내었다. 결론적으로, 키토산을 경구 투여한 경우에서 인간에게 독성을 유발하지 않을것으로 사료되었다. Chitin is the second most abundant natural biopolymer in the world, next to cellulose, contains amino sugars which is found as a component of crustacean shells. Chitosan is a deacetylated product of chitin and it has been applied in the field of healthy food and other industries. This study was purposed to investigate the toxicity of natural chelator chitosan for the safe utilization. The blood cell composition and chromosomal aberration were studied after chitosan and EDTA feeding in mice. There was no specific differences at the changes of blood cell numbers and micronucleus numbers between chitosan feeding and normal feeding state. General chitosan prepared for food did not show genetic aberrations. Natural chelator chitosan was more stable in the blood cell numbers and chromosomal aberration. With this result, the study on the natural chelator of chitosan is needed and the clinical application is recommended. The cytogenetic toxicity of the natural chelator, chitosan was investigated comparing with the chemical chelator, EDTA in male ICR mice. Chitosan and EDTA powder food were supplied orally to mice for one week. In the blood components, micronucleus numbers and chromosomal aberration, chitosan groups did not appear any aberration. On the other hand, high toxicities were observed in the EDTA groups throughout the experimental period. As the result, the oral treatment of chitosan estimated to have no side effects in humans from the results in this study.