HaCaT 세포주에서 Epigallocatechin-3-Gallate (EGCG)가 자외선 및 LPS에 의한 iNOS mRNA 발현 및 NO 생성에 미치는 영향

탁우정 ( Tag U Jeong ),이창균 ( Lee Chang Gyun ),서성준 ( Seo Seong Jun ),김명남 ( Kim Myeong Nam ),노병인 ( No Byeong In ),홍창권 ( Hong Chang Gwon ) 대한피부과학회 2004 대한피부과학회지 Vol.42 No.1

N/A Background: Nitric oxide (NO) plays an important role in inflammation and multiple stages of carcinogenesis. Green tea (Camellia sinensis polyphenols are potent antiinflammatory agents and have been shown to inhibit NO production in tumor cell lines. In the present study, we examined the effect of epigallocatechinp-3-gallate (EGCG), a generation of NO in HaCaT cells. Methods: HaCaT cells were treated with 10μM EGCG and 100μM NAC for 1 hour. 1 hour later, they were irradiated with 50mJ/cm^2 UVB and treated with 200 u㎍/ml LPS for 12 hours, respectively. The iNOS mRNA was determined by reverse transcription-polymerase chain reaction (RT-PCR) and NO production was assessed by spectrophotometric method based on Griess reaction. Nuclear factor κB (NF-κB) binding activity was determined by electophoretic mobility shift assay (EMSA). Results: The results were as follows 1. EGCG inhibited UVB and LPS induced expression of inducible nitric oxide synthase. 2. HaCaT cells cotreated with EGCG produced significantly less iNOS mRNA and NO compared with HaCaT cells stimulated with UVB irradiation of LPS. 3. The inhibition of iNOS mRNA and NO production correlated with the suppression of expression of NF-κB dependent gene iNOS. 4. EGCG inhibited the activation and translocation of NF-κB to the nucleus Conclusion: inhibited the activation and translocation of NF-κB to the nucleus in HaCaT cells by interfering with the activation of NF-κB through a novel mechanism. Our results further suggest that EGCG may be therapeutically effective in UVB and cytokine induced cutaneous inflammation. (Korean J Dermatol 2004;42(1):37~46)

산양유 쿠미스의 제조와 Candida kefir 의 젖산 생성

인영민,정석근,함준상,안영태,김현욱,김동운,김회발,김용곤 한국유가공기술과학회 2000 Journal of Dairy Science and Biotechnology (JMSB) Vol.18 No.2

Goat milk consumption in Korea has been increasing nowadays due to recognition of good for health, and some goat milk producing farms get high income by processing and selling. Some chemical, physical, and nutritional characteristics of goat milk were reviewed. Development of various types of goat milk products is required for both producer and consumer, and Koumiss could be a good application. Koumiss is lactic acid-alcohol fermented milk product and is widely used for treating pulmonary tuberculosis in Russia. Koumiss making from goat milk was compared to from cow milk. L. plantarum and C. kefir isolated from Mongolian Koumiss were used as starter cultures, and mixed fermentation with L. bulgaricus showed symbiotic growth effect. The best sensory characteristics were obtained in two day fermented Koumiss. However the Koumiss making procedure was very complex because of mixed fermentation of yeast and lactic acid bacteria. To simplify this complex procedure, the yeast was developed to express 1dh gene of the lactic acid bacteria. L-1dh gene was isolated from the L. plantarum by PCR, and ligated to a commercial shuttle vector, The vector containing L-1dh was transformed to E. coli, and was purified after large scale preparation. The purified vector was transformed to C, kefir by electroporation after removal of antibiotic marker. Two kinds of colonies were obtained on YPG agar containing bromocresol purple, and the colony which shows yellow surrounding was considered to express 1dh gene. Expression conditions of the 1dh gene in the yeast need further study.

살 오징어(Todarodes pacificus) 간췌장 유래 한외여과 Aminopeptidase Retentate Fraction의 특성과 쓴맛 개선효과

김진수 ( Jin-soo Kim ),이정석 ( Jung Suck Lee ),윤인성 ( In Seong Yoon ),강상인 ( Sang In Kang ),박선영 ( Sun Young Park ),정우철 ( U-cheol Jeong ),허민수 ( Min Soo Heu ) 한국수산과학회(구 한국수산학회) 2020 한국수산과학회지 Vol.53 No.1

This study investigated some enzymatic properties and bitterness improvement of an aminopeptidase retentate fraction (ARF) from common squid Todarodes pacificus hepatopancreas extract (HPE), obtained by ultrafiltration with a 10 kDa molecular weight cut off membrane. Endoprotease and aminopeptidase (AP) activity, and the purity of the ARF (>10 kDa) increased by 6.69-18.11 U/mg and 1.5-2.6 fold, respectively, compared to HPE (2.63-9.37 U/mg). The AP activity toward LeuPNA was stable at 20-55°C and pH 5-9, but decreased slightly with increasing concentration of NaCl in the reaction mixture. The ARF was the most active MetPNA and preferentially hydrolyzed Glu, Leu and AlaPNA. The bitterness tryptic casein hydrolysates (BTCHs) were treated with ARF, and the bitterness of ARF-BTCHs significantly decreased with increasing amounts of released amino acids Ala, Val, Met, Ile and Leu, which show strong correlations with bitterness. Therefore, the ARF of T. pacificus HPE obtained by ultrafiltration may have a considerable potential for application in protein hydrolysis and appears to be ideally suited to the purpose of lowing bitterness in protein hydrolysates.

폐경후 여성에서 2개월간의 호르몬대치요법이 혈청 지질과 Apolipoprotein(a)의 농도에 미치는 영향

민용기 ( Min Yong Gi ),장학철 ( Jang Hag Cheol ),이관우 ( Lee Gwan U ),한인권 ( Han In Gwon ),김치정 ( Kim Chi Jeong ) 대한내과학회 1993 대한내과학회지 Vol.44 No.2

연구배경 : 폐경후 심혈관질환이 증가하며, 에스트로겐을 투여하면 이으 발생이 현저히 감소한다. Apolipoprotein(a)[apo(a)]는 lipoprotein(a)[Lp(a)]를 이루고 있는 당단백질이며 Lp(a)는 죽상경화증의 독립적인 위험인자라는 것이 밝혀져 있다. 폐경기 여성의 혈청apo(a)의 농도를 감소시키기 위한 많은 연구들이 있었으나, 호르몬 대치요법이 폐경기 여성의 혈청apo(a)의 농도에 미치는 영향은 잘 알려져 있지 않다. 이에 저자들은 폐경후 여성에서 2개월간의 에스트로겐 투여에 의한 혈청 지질 및 apo(a)농도의 변화와 이러한 변화에 프로게스토겐 병합요법이 어떠한 영향을 미치는가를 살펴보고자 전향적 연구를 시행하였다. 방법 : 혈청 FSH 농도가 40mIU/ml 이상이거나, 마지막 월경후 12개월 이상이 경과된 여성 272명을 대상으로 하였다. 자궁적출술을 시행하지 않은 여성은 무작위로 group A와 group B의 2군으로 나누어 group A는 25일 간 conjugated equine estrogen (CEE) 0.625mg과 후반 10일간 medroxyprogesterone acetate(MPA) 10mg을 병합 투여하였다. Group B는 25일간 CEE 0.625mg과 후반 10일간 MPA 5mg을 병합 투여하였다. 자궁적출술을 시행한 group C는 CEE 0.625mg 만을 25일간 경구 투여하였다. 각 group에서 이후 5일간은 투약을 중지하였으며 이를 매월 반복하였다. 혈청 콜레스테롤, 중성지방, 저밀도지단백질 콜레스테롤, 초저밀도지단백질 콜레스테롤, 고밀도지단백질 콜레스테롤, apo(a)의 농도를 측정하여 치료전후를 비교하였다. 결과 : 혈청 총콜레스테롤의 농도는 치료전 group A, B, C에서 각각 5.42±0.09mmol/L, 5.59±0.09mmol/L, 5.53±0.13mmol/L 이었고 2개월간의 호르몬대치요법후 group A, B에서 각각 4.93±0.08mmol/L, 5.22±0.08mmol/L로 통계적으로 유의하게 감소하였다(p=0.001). 혈청 저밀도지단백질 콜레스테롤의 농도는 치료전 group A, B, C에서 각각 3.29±0.07mmol/L, 3.41±0.07mmol/L, 3.40±0.10mmol/L 이었고 치료후 group A, B. C에서 각각 3.00±0.06mmol/L(p=0.0001), 3.14±0.06mmol/L, (p=0.0001) 3.18±0.12mmol/L(p=0.05)로 통계적으로 유의하게 감소하였다. 혈청 고밀도지단백질 콜레스테롤의 농도는 치료전 group A, B, C에서 각각 1.72±0.04mmol/L, 1.75±0.04mmol/L, 1.69±0.06mmol/L 이었고 치료후 group C에서만 1.90±0.07mmol/L로 통계적으로 유의하게 증가하였다(p=0.0001). 혈청 중성 지방의 농도는 치료전 group A, B, C에서 각각 1.45±0.08mmol/L, 1.54±0.08mmol/L, 1.68±0.15mmol/L 이었고 치료후 group A, B, C에서 모두 통계적으로 유의한 변화는 없었다. 혈청 초저밀도지단백질 콜레스테롤의 농도는 group A, B, C에서 각각 치료전 0.39±0.03mmol/L, 0.40±0.03mmol/L, 0.45±0.05mmol/L 이었고 치료후 group A, B에서 각각 0.30±0.02mmol/L(p=0.0001), 0.34±0.03mmol/L(p<0.05)로 통계적으로 유의하게 감소하였다. 혈청 apo(a) 농도는 group A, B, C에서 각각 치료전 357.5±33.2U/L, 348.7±28.22U/L, 376.8±54.2U/L 이었고 치료후 group A, B, C에서 각각 264.7±26.1U/L, 291.0±31.8U/L, 228±47.4U/L로 모든 group에서 통게적으로 유의하게 감소하였다(p=0.0001). 결론 : 폐경후 여성에게 호르몬 대치법을 시행하는 것은 혈청 지질과 apo(a)에 유익한 효과를 보이고 있으며, 따라서 심혈관질환 예방의 중요성을 감안할 때 폐경후 여성에게 호르몬대치요법을 시행하는 것이 바람직한 것으로 사료된다. Background : Postmenopausal women have a high risk of cardiovascular disease than premenopausal women and hormone replacement therapy [HRT] reduces the risk of cardiovascular disease. Apolipoprotein(a)[apo(a)] is attached by disulfide linkage to apo B-100 of LDL, forming lipoprotein(a)[Lp(a)]. The level of this lipoprotein is an independent risk factor for atherosclerosis. The serum level of apo(a) is stable and not affected by most dietary interventions and lipid lowering agents. There was little information available about the effect of HRT on levels of Lp(a). This study was conducted to assess the effect of HRT on serum lipids and apo(a) levels in postmenopausal women. Methods : Postmenopausal women with serum FSH level above 40mIU/ml or no menstruation for more than I year were included in the study. They received the combination of conjugnated equine estrogen (CEE) 0.625mg a day for 25 days plus medroxyprogesterone acetate 10mg(group A)or 5mg (group B) a day for the last 10 days followed by 5 days off. Hysterectomized women received CEE 0.625mg a day for 25 days (group C). HRT was repeated for 2 months. The baseline levels of serum total cholesterol, triglyceride, LDL cholesterol, VLDL cholesterol, HDL cholesterol, and apo(a) were compared with posttreatment levels. Results : Serum total cholesterol levels before treatment were 5.42±0.09mmol/L, 5.59±0.09mmol/L, 5.53±0.13mmol/L in group A, B, C respectively. After HRT they were significantly reduced to 4.93±0.08mmo/L, 5.22±0.08mmol/L in group A, B respectively (p=0.0001). Serum LDL cholesterol levels before treatment were 3.29±0.07mmol/L, 3.41±0.07mmol/L, 3.40±0.10mmol/L in gruop A, B, C respectively. After HRT they were significantly reduced to 3.00±0.06mmol/L (p=0.0001). 3.14±0.06mmol/L(p=0.0001), 3.18±0.13mmol/L(p=0.05) in gruop A, B, C respectively. Serum HDL cholesterol levels before treatment were 1.72±0.04mmol/L, 1.75±0.04mmol/L, 1.69±0.06mmol/L in gruop A, B, C respectively. After HRT they were significantly increased to 1.90±0.07mmol/L in gruop C (p=0.0001). Serum triglyceride levels before treatment were 1.45±0.08mmol/L, 1.54±0.08mmol/L, 1.68±0.15mmol/L in gruop A, B, C respectively. After HRT they were not significantly changed. Serum VLDL cholesterol levels before treatment were 0.39±0.03mmol/L, 0.40±0.03mmol/L, 0.45±0.05mmol/L in gruop A, B, C respectively. After HRT they were significantly reduced to 0.30±0.02mmol/L(p=0.0001), 0.34±0.03mmol/L (p<0.05) in gruop A, B respectively. Serum apo(a) levels before treatment were 357.5±33.2U/L, 348.7±28.2U/L, 376.8±54.2U/L in group A, B, C repectively. After HRT they were significantly reduced to 264.7±26.1U/L, 291.0±31.8U/L, 228.8±47.4U/L in group A, B, C reapectively (p=0.0001) Conclusion : These results suggest that HRT for 2 months in postmenopausal women has beneficial effect on serm lipids and apo(a) levels and HRT in postmenopausal women can improve their cardiovascular risk.

고립성 폐결절에 관한 임상적 고찰

이종인 ( Lee Jong In ),이상수 ( Lee Sang Su ),원구태 ( Won Gu Tae ),안강현 ( An Gang Hyeon ),이성우 ( Lee Seong U ),백순구 ( Baeg Sun Gu ),김승률 ( Kim Seung Lyul ),용석중 ( Yong Seog Jung ),신계철 ( Sin Gye Cheol ),정순희 ( Jeong 대한내과학회 1993 대한내과학회지 Vol.44 No.2

N/A Background : Because there aremany different reporting results about the etologies of solitary pulmonary nodules (SPNs), we cannot know the exact incidence of each causative disease. SPNs have a good prognosis relatively even if it is a malignant lesion. In Korea, where there is a high incidence of pulmonary tuberculosis, the differential diagnosis of SPNs should be performed. Methods : Among 351 patients who visited Wonju Chistian Hospital for SPNs in chest x-ray between 1982 and 1989, we evaluated 74 patients in whom the causes of SPNs could be elucidated by sputum AFB study, cytology, histolgic study, chest of solitary pulmonary nodule (SPN) seen at the Wonju Christian Hospital from 1982 to 1989. The followings were obtained. 1) The malignancies of SPN 74cases were 25 cases (33.7%). The primary lung carcinomas were 24 cases : The epidermoid carcinomas of these were 11 cases (45.8%), metastasis I case, tuberculoma 42 cases, and the other 7 cases. 2) The age distribution of benign lesions was from 19 to 73 years old with the mean age of 46 years old, and the age distribution of malignancies was from 17 to 77 years old with the mean age of 57 years old. The 23 cases (92.0%) of malignant nodules were more than 40 years old. The malignant frequency of SPN in a group of age more than 40 years old was 40.3% and in a group of age less than 40 years old, it was 11.7%. The malignant frequency was directly proportional to the increase of age(p<0.05). 3) The 3 cases of SPN were detected accidentally during physical up, all were benign. The 14 cases were detected during the evaluation of other diseases, 12 cases were benign. The 23 cases (40%) of 57 cases were symptomatic. Chest pain, dyspnea, and weight loss frequency in a group of less than 3cm in size was 11.9% and in a group of less than 3cm in size, it was 62.5% (p<0.05). 6) Calcifications were noted in 15 cases of 74 cases on the plain films, all were 7 central types, 7 diffuse types and 1 popcorn type. One of malignant cases had calcification on the chest CT scar. Among the 59 cases with non-calcified nodules, 25 cases were malignant (42.3%). The mean Hounsfield Unit of benign lesions was 182.5U and that of malignancies was 94U, there was a statistical difference between both groups(p<0.01). 7) The 21 cases (50%) of tuberculomas were located at the apicoposterior segment of upper lobe or the superior segment of lower lobe, but the 7 cases (28%) of malignancies were located at above sites. 8) The diagnostic accuracy of sputum study was 9.4% and that of fiberoptic bronchoscopy (FOB) was 33.3%. The diagnostic accuracy of transbronchial lung biopsy (TBLB) and transthoracic needle aspiration biopsy (TTNAB) was 57.4%. The percentage of primary lung cancer among SPNs which are larger than 3cm in size, occurring in the age of older than 40 years old and with no calcifications on radiologic examintion should be suspected as malignancies and diagnosed with sputum study, fiberoptic bronchoscopy, TBLB, and TTNAB.

바이러스성 및 알코올성 간질환에서의 장투과성의 변화

김정욱 ( Kim Jeong Ug ),전우규 ( Jeon U Gyu ),윤중원 ( Yun Jung Won ),박동일 ( Park Dong Il ),조용균 ( Jo Yong Gyun ),성인경 ( Seong In Gyeong ),박창영 ( Park Chang Yeong ),손정일 ( Son Jeong Il ),김병익 ( Kim Byeong Ig ),김은정 ( 대한소화기학회 2004 대한소화기학회지 Vol.43 No.2

Background/Aims: Increased intestinal permeability has been possible contributing factors to the pathogenesis of alcoholic liver disease. Moreover, it can contribute to the development of bacterial infection and intestinal endotoxemia in patients with liver cirrhosis. This study aimed to examine the difference of intestinal barrier dysfunction between alcoholic and viral liver disease patients through the comparison of the intestinal permeabilities of patients with clinical characteristics. Methods: Intestinal permeabilities were measured in 18 healthy controls, 41 patients with alcoholic liver disease (17 cases of alcoholic liver disease without cirrhosis and 24 cases of alcoholic liver cirrhosis) and 46 patients with viral liver disease (14 cases of chronic viral hepatitis and 32 cases of viral liver cirrhosis) by measuring 24 hour urine excretion of 51Cr-EDTA. Results: The intestinal permeability was significantly increased in the patients with alcoholic liver disease without cirrhosis (5.62±80%), alcoholic liver cirrhosis (5.29±48%) and viral liver cirrhosis (3.15±39%) compared with that in control subjects (1.99±53%). On the contrary, it was not increased in the patients with chronic viral hepatitis (2.05±57%) versus controls. The significant correlation was not found between intestinal permeability and clinical and laboratory findings. Conclusions: The intestinal permeability was elevated in patients with alcoholic liver disease compared to those with viral liver cirrhosis. The pathophysiology of liver injury secondary to intestinal epithelial damage may be different between alcoholic and viral liver diseases. (Korean J Gastroenterol 2004; 43:104-111)

위암에서 CD40의 발현 및 CD40이 위암 세포의 아포토시스의 미치는 영향

김상우 ( Kim Sang U ),이학성 ( Lee Hag Seong ),윤승규 ( Yun Seung Gyu ),정우철 ( Jeong U Cheol ),조영석 ( Jo Yeong Seog ),정정조 ( Jeong Jeong Jo ),이인석 ( Lee In Seog ),이강문 ( Lee Gang Mun ),이동수 ( Lee Dong Su ),최명규 ( Cho 대한소화기학회 2003 대한소화기학회지 Vol.42 No.4

Reliability of Dynamic Modulus measurement using ultrasonic sound velocity and acoustic resonance frequency

Jeong-Eun Lee(이정은),Seung-Hyeon Song(송승현),Ji-U Ha(하지우),Hyo-Ri Kim(김효리),Chang-Soon Lee(이창순),In-Sik Cho(조인식) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.11

The elastic modulus and Poissons ratio have been measured by the tensile test with the attachment of gauge sensors. However, the simpler dynamic method has been developed by using the acoustic resonance frequency, which is much easier and more accurate than the conventional tensile test. The typical dynamic methods are (i) the Ultrasonic pulse echo method, (ii) the Resonance frequency method, and (iii) the Impulse Excitation Method. Although both the Ultrasonic echo method and the resonance frequency method have widely been used, the Impulse Excitation Method has come into the spotlight due to its simplicity and accuracy, which has also been prescribed in ASTM E1876. In this study, the practical control system has been implemented with the numerical analysis algorithm for the Impulse Excitation Method. With this technique, the dynamic elastic modulus and Poissons ratio have been measured and evaluated for structural materials such as Al7075-T6, STS304, STS420_J2, SCM440, STS630 and Ti6Al4V. Therefore, the reliability was verified through comparative evaluation of the results of the static test method and the dynamic test method for six structural materials.

영상처리를 위한 웨이브렛 변환 디지털 필터의 설계

김윤홍,전경일,방기천,이우순,박인정,이강현,Kim, Yun-Hong,Jeon, Gyeong-Il,Bang, Gi-Cheon,Lee, U-Sun,Park, In-Jeong,Lee, Gang-Hyeon 대한전자공학회 2000 電子工學會論文誌-CI (Computer and Information) Vol.37 No.3

In this paper, we proposed the hardware architecture of wavelet transform digital filter for an image processing. Filter bank pyramid algorithm is used for wavelet transform and each fillet is implemented by the FIR filter. For DWT computation, because the memory controller is implemented by hardware, we can efficiently process the multisolution decomposition of the image data only input the parameter. As a result of the image Processing in this paper, 33㏈ PSNR has been obtained on 512$\times$512 B/W image due to 11-bit mantissa processing in FPGA Implementation. And because of using QMF( Quadrature Mirror Filter) properties, it reduces half number of the multiplier needed DWT(Discrete Wavelet Transform) computation so the hardware size is reduced largely. The proposed scheme can increase the efficiency of an image Processing as well as hardware size reduced. The hardware design proposed of DWT fillet bank is synthesized by VHDL coding and then the test board is manufactured, the operating Program and the application Program are implemented using MFC++ and C++ language each other. 본 논문에서는 영상처리를 위한 웨이브렛 변환 디지틀 필터 설계의 하드웨어 구조를 제안한다. 웨이브렛 변환을 위하여 필터 뱅크 피라미드 알고리즘을 이용하고 각각의 필터는 FIR 필터로 구현하였다. 그리고 메모리 제어기를 하드웨어로 구현하여 DWT 계산이 수행되므로 단순한 파라미터 입력만으로 영상 데이터의 다중해상도 분해를 효율적으로 처리할 수 있었다. 본 논문에서의 영상처리 결과는 FPGA의 하드웨어적 제한으로 인한 11bit의 가수처리 때문에, 512×512 흑 백영상에 대하여 33㏈의 PSNR이 나타났다. 그리고 QMF(Quadrature Mirror Filter)의 특성을 이용하여 DWT(Discrete Wavelet Transform) 계산에 필요한 승산기의 수를 절반으로 줄임으로써 하드웨어의 크기도 감소하였다. 그러므로 제안된 방법은 하드웨어 크기의 감소에 따른 영상처리의 효율성을 증대할 수 있다. DWT 필터 뱅크의 제안된 하드웨어 설계는 VHDL 코딩으로 설계합성을 하여 테스트 보드가 제작되었으며, 실행프로그램은 MFC++로, 영상복원 디코드 응용프로그램은 C++언어를 이용하여 구현하였다.

자기조립법에 의한 단분산 콜로이드의 배열

정인우(Jeong In U),이정민(Lee Jeong Min),김중현(Kim Jung Hyeon) 한국고분자학회 2004 고분자 과학과 기술 Vol.15 No.1