인체종양세포주들에 대한 재조합 감마인터페론의 세포살해능, 증식억제능, HLA항원 표현에 관한 연구

송형근,박성희,김노경,이상국 대한의학협회 1990 대한의학협회지 Vol.33 No.3

실험 방광 종양화과정에서 Proliferating Cell Nuclear Antigen(PCNA)의 발현

김원재,송형근,이현무 충북대학교 의과대학 충북대학교 의학연구소 1992 忠北醫大學術誌 Vol.2 No.1

Fisher 344백서에 0.1% N-butyl-N-(4-hydroxybutyl) nitrosamine(BBN)을 투여하여 방광에 단순과증식(simple hyperplasia ; SH), 결절성 혹은 유두상 과증식(nodular or papillary hyperplasia ; NPH) 및 표재성 방광암(Ta, T1)을 유발시킨 후 개개의 단계의 증식성의 차이를 보고자 후기 G1 phase, S-phase 및 전기 G2/M phase를 반영하는 proliferating cell nuclear antigen(PCNA/cyclin)에 대한 anti-PCNA/cyclin monoclonal antibody를 이용하여 세포의 labeling index(LI)를 구하였다. 정상,SH,NPH,Ta 및 T1에서의 LI는 0.76±2.71,4.52±7.74,10.55±17.99,16.97±18.66, 17.04±16.10이었으며, grade I이 14.54±16.21, grade II가 19.30±16.74이었다. 방광암의 종양화 과정이 진행함에 따라 통계적으로 유의하게 LI가 증가하는 양상을 보였으며 또한 분화도가 나빠질수록 LI가 유의하게 증가하였다. 그러나 종양의 경우 즉, Ta와 T1사이에는 통계적으로 유의한 차이(P>0.4)가 관찰되지 않았다. 이러한 사실은 이들의 경우에 있어서 세포분화도상 비슷한 병기의 표재성 종양이기 때문에 LI의 차이가 없었던 것으로 생각되며 이같은 세포역동학적 분석방법에 있어서는 종양의 병기보다는 개개의 세포의 생물학적 악성도에 의해서 좌우되는 것 같다. Proliferating cell nuclear antigen(PCNA)/cyclin is a cell-cycle-regulated protein, which can be demonstrated in routinely fixed specimens. The expression of PCNA/cyclin was investigated immunohistochemically in BBN induced rat bladder tumorigenesis using anti-PCNA/cyclin monoclonal antibody in embedded tissue fixed with formalin. Sequential cellular changes(normal, simple hypernlasia, nodular or papillary hyperplasia, and transitional cell carcinoma-Ta, T1) were observed in rat urinary bladder in accordance with the duration of BBN administration. The PCNA labeling index(LI) was determined by counting the number of PCNA labeled cells in the corresponding tissue section. The average PCNA LI obtained using this method in normal, simple hyperplasia(SH), nodular or papillary hyperplasia(NPH), and bladder tumor(Ta, T1) was 0.76, 4.52, 10.55, and 17.01(16.97, 17.04), respectively. Also, higher PCNA LI was found in grade II compared to grade I. Higher PCNA LI indicated greater biological malignancy based on the fact that mean LI progressively increased with tumorigenesis. However, mean LI at Ta did not significantly differ from that of T1, which implied that Ta might reflect similar cellular characteristics as a superficial bladder tumor.

전치부 개방교합자와 정상교합자의 이설근 및 구륜근 활성도에 관한 비교 연구

강용,송형근,윤영주,김광원 대한치과교정학회 1995 대한치과교정학회지 Vol.25 No.2

이 연구는 정상 교합자와 개방교합자 사이에, 측모두부방사선계측사진 분석을 하고, 이설근과 구륜근의 활성도를 비교하여 근활성도와 골격의 형태간에 상호 상관 관계를 알고자 하였다. 연구 대상으로는 전신건강 상태가 양호하고, 교정치료 경험이 없으며, 악관절 장애가 없는 Angle Ⅰ급 교합관계를 가진 20명의 정상교합자와 overbite가 -0.5에서 -6mm인 19명의 개방교합자를 선정하였으며, 각 대상에서 측모두부방사선계측사진을 촬영하여, 20가지 항목을 계측하고, 안정위, 연하시, 개구시, 등장성 혀 내밀기, 최대 혀 내밀기때의 이설근과 구륜근의 근활성도를 관찰하고, 근활성도와 골격의 형태간에 상관관계를 알고자 하였다. 이 연구로부터 얻어진 결과는 다음과 같다. 1. 안정위를 제외하고, 이설근의 근활성도는 개방교합자에서 정상교합자보다 유의성있게 높았다. 2. 연하시를 제외하고, 구륜근의 근활성도는 개방교합자에서 정상교합자보다 유의성있게 높았다. 3. 최대 혀 내밀기에서 개방교합자의 이설근이 가장 높은 근활성도를 보였다. 4. 골격형태와 이설근과 구륜근의 상관관계가 정상교합군에서는 적고, 개방교합군에서는 더 많은 항목에서 유의성있는 상관관계가 있었다. This study was undertaken to investigate the activities of genioglossus and orbicularis oris muscle between occlusion and anterior open bite group. 39 subjects without the experience of orthodontic treatment and T.M.disorder were selected for this study. 20 subjects were normal occlusion, 19 subjects were anterior open bite. The twenty items were measured from the cephalometric headplates, and EMG recording of the genioglossus, orbicularis oris muscle were taken at rest position., water swallowing, jaw opening, isometric tongue protrusion, maximum tongue protrusion. All data were analyzed and processed with the computer statistical method. The following results were obtained : 1. Except at rest position, the muscle activities of genioglossus muscle in anterior open bite were higher than in normal occlusion with significatn difference. 2. Except druing water swallowing, the muscle activities of orbicularis oris muscle in anterior open bite were higher than in normal occlusion with significant difference. 3. During maximum tongue protrusion, the geniolossus muscle of anterior open bite subjects showed the highest muscle activity. 4. Anterior open bite showed closer interrelationship between facial morphology ad the genioglossus, orbiculars oris muscle activities than that of normal occlusion with significatn difference.

Castleman 질환 1례

안묘순,송형근,박범수,한헌석,하태선 충북대학교 의과대학 충북대학교 의학연구소 1993 忠北醫大學術誌 Vol.3 No.1

Castleman 질환은 주로 종격동 임파선을 침범하여 임상적, 조직학적으로 흉성종과 유사한 소견을 보이는 질환이다. 보통 침범된 임파선은 3cm에서 7cm의 크기를 보이며 경계가 뚜렷한 종양으로 나타난다. 조직학적 소견상 hyaline-vascular형(H-V형)과 plasma cell형의 두가지로 나눈다. 임상적으로는 hyaline-vascular형은 별증상이 없으며 보통 우연히 발견되고 드물게 기도와 기관지를 압박하는 증세를 보이기도 한다. plasma cell형은 빈혈, 적혈구침강속도의 증가, 고감마글로불린혈증, 저알부민혈증, 그리고 저트란스페린혈증을 보이기도 한다. 저자들은 생후 2개월부터 반복되는 호흡기 감염과 거친 숨소리를 보이면서, 성장 및 발육장애를 보인 14개월된 남아에서, 기도에서 우측 주기관지까지를 압박하고 있는 종양을 흉부 전산화단층촬영에서 발견하고 떼어낸 후 조직소견상 hyaline-vascular형의 giant lymph node hyperplasia의 소견을 보이는 Castleman 질환 1례를 경험하였기에 보고하는 바이다. Castleman's disease(angiofollicular lymph node hyperplasia) can arise from lymph nodes or extranodal tissues and can be classified histologically into the hyaline-vascular form, characterized by hyaline follicle-like structures interspersed with vascular tissue, or the plasma cell variant, in which the follicle-like structures are seperated by sheets of plasma cells amidst vascular interfollicular tissue. The clinical manifestations of the multicentric variant can be protean and inculude fever, night sweats, weight loss, lymphadenopathy, hepatosplenomegaly, pleural effusion, ascites, anemia, thrombocytopenia, polyclonal hypergammaglobulinemia, and neurological and renal abnormalities. We report a case of Castleman's disease in 14-month old male patient with the chief complaints of recurrent respiratory symptoms and delayed growth and development, who was diagnosed as having a hyaline-vascular form of above disease after the excision of pulmonary mass which compressed the right main bronchus.

삼차신경 감각핵의 세포활성도와 Substance P 유리에 대한 Capsaicin, Paradol 및 Shogaol의 전통작용기전에 관한 연구 : Ⅰ. 유해자극에 의한 개구반사와 삼차신경 척수감각핵의 Substance P에 미치는 영향 Ⅰ. EFFECTS ON JAW OPENING REFLEX BY NOXIOUS STIMULUS AND RELEASE OF SUBSTANCE P

이종흔,김중수,송형근,박수정,함진숙 대한구강생물학회 1990 International Journal of Oral Biology Vol.14 No.1

The purpose of this investigation was to determine the relative analgesic effectiveness of paradol and shogaol compared with capsaicin, and effects of capsaicinoids on substance P of trigeminal spinal sensory nucleus. Under anesthesia, anterior belly of digastric muscle was exposed and a pair of electrodes was inserted to record the electromyograms. Noxious electrical stimulus was applied to oral mucosa around the mental foramen. Inferior alveolar nerves (IAN) and saphenous nerves were carefully exposed for application of vehicle and drugs to evaluate the effects on digastric EMG (dEMG) and neural conduction, and caudal part of brainstem was exposed to determine the substance P. Vehicle and 1.5% of capsaicin, paradol and shogaol were applied to IAN and saphenous nerves for 30 minutes, respectively. EMG_s of digastric muscle evoked by noxious electrical stimulus were recorded before application of drugs or vehicle, immediately after removal and washing of drugs and at 30 minutes after washing. To examine the effects of the peripheral stimulation on the action potential, stimulating electrodes were placed on the peripheral side of drugs application site and recording electrodes were on the proximal side. Substance P of trigeminal spinal sensory nucleus was collected by push-pull perfusion technique and determined by radioimmunoassay. Capsaicin application on IAN could not influence on dEMG, but paradol and shogaol decrease the amplitude of dEMG. Capsaicin applied locally to nerve decreased amplitude and conduction velocity of only C-fiber, while paradol and shogaol inhibited the neural conduction of C-fiber and Aδ-fiber. Substance P in the trigeminal spinal nucleus was reduced by capsaicin but not by paradol and shogaol.

저빈도 전기자극이 개의 치아 및 치은에 대한 동통억제효과

권훈,이상호,송형근 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.2

The purpose of this study was to investigate the effect of electical anesthesia induced by non-acupuncture point stimulation on inhibition of amplitude of digastric EMG evoked by noxious electical stimuli in teeth and gingiva. Experiments were performed with dogs anesthetized with intraperitoneaal pentobarbital sodium in an initial dose of 30mg/kg.Maintenance doses of 4.0ml/hout were given through a cannula in the femoral vein using a constant infusion pump. Anterior belly of digastric muscle was exposed and a pair of 0.1mm wire electrodes were inserted for E.M.G. recording.Bipolar electrodes were inserted into the labial and lingual surface of upper canine and the labial area of upper gingiva.Noxious stimuli were delivered to the tooth and gingiva through those electrodes by electric stimulator. Non-acupuncture point stimulation of 2Hz was delivered bilaterally to the femoral area.Amplitudes of digastric E.M.G. were measured from the oscilloscope and monitor connected to amplifier at different intensities fo electronic anesthesia of 1 volt,4 volt and 10 volt.The inhibited rate of the amplitudes of digastric E.M.G. were analysed statistically with paired t-test. The following results were obtained: 1.Non-acupuncture point stimulation with intensities of 1 volt,4 volt and 10 volt showed the inhibitory effect on pain of 15%,25%,and 16% in teeth and 15%,18% and 12% in gingiva respectively. 2.In tooth,statistical significance was observed between control and each group.In gingiva,there was statistical significance between control and group 1,2 except group 3. From these results,low frequency electrical stimulation of non-acupuncture point resulted in reducing of dental and gingiva pain,it could be used as adjunct to other pain control methods.

기도저항 변화가 설골근 활동성에 미치는 영향

우종윤,이상호,송형근 大韓小兒齒科學會 1995 大韓小兒齒科學會誌 Vol.22 No.1

There are some morphological changes in chronic mouth breathers, for example, a clock-wise rotation of mandible, extended posture of head, a lowered position of the tongue and the hyoid bone. When the intraluminal airway pressure becomes negative by the increase in airway resistance during inspiration, the airway can be collapsed. It was reported that hyoid position has a key role for maintaining airway patency. Anterior movement of hyoid bone can widen the airway and thus prevent airway obstruction. Many hyoid muscles attached to hyoid bone might control the position of hyoid. The present study was, therefore, designed to investigate the changes of activity of some hyoid muscles and the control mechanisms during increase in airway resistance. We obstructed the trachea or applied the sustained negative pressures to the isolated upper airway in 10 spotaneously breathing anesthetized dogs. The peak amplitudes of rectified and integrated eletromyogram(EMG)s from the geniohyoid, sternohyoid and sternothyroid were recorded. 1. As airway resistance increased, airway pressure were decreased and the peak amplitudes of integrated EMGs from those muscle were increased. Activities of all 3 muscles were increased during the first occluded breathing when the airway was occluded in the end of expiration. 2. The peak amplitudes of EMGs from those muscles were increased during applications of the negative pressure in the isolated upper air way. We could find these changes in the next breathing from pressure application. These results indicate that the negative pressure generated in upper airway augments the activities of hyoid muscles through the mechano-receptor activation and so does the deflation of lung. We speculate that geniohyoid and infrahyoid muscles cooperate for the anterior and inferior movement of hyoid bone.

장벽경색이 선행한 Henoch-Schonlein 자반증 1례

한헌석,하태선,송형근,이상전,안묘순,박범수 충북대학교 의과대학 충북대학교 의학연구소 1993 忠北醫大學術誌 Vol.3 No.1

Henoch-Schonlein 자반증은 피부, 위장관, 관절, 신장 등을 주로 침범하며 소혈관염을 기본 병리 소견으로하는 전신성 혈관 장애 질환이다. 위장관계 증상으로는 복통, 오심 및 구토, 잠혈, 토혈, 흑색변 등이 있을 수 있고, 이러한 증상은 합병증 없이 회복되는 것이 보통이나 드물게는 장중첩증, 장벽경색 및 괴사, 장천공 등의 심한 합병증을 동반할 수도 있다. 이러한 위장관계 증상들은 특징적인 피부 소견에 선행하여 나타날 수도 있으며, 이때는 급성복증에 대한 감별진단이 중요하다. 저자들은 장벽경색이 선행하여 감별진단에 어려움을 겪고 시험적개복술을 시행한 이후에 특징적인 피부 자반이 발현되어 Henoch-Schonlein 자반증으로 진단할 수 있었던 증례를 경험하였기에 이를 보고하고자 한다. 본 증례는 입원 1일전부터 시작된 복통을 주소로 입원한 5세 남아가 배꼽 주위에 심한 경련성 복통을 지속적으로 호소하였고 이학적 소견상 배꼽주위의 압통, 장음의 감소, 흑색변 등이 있었으며 소장조영술상 공장 부위에 미만성의 불규칙한 점막비후 소견을 보여 제 8병일에 시험적개복술을 시행한 결과 근위 공장에 국한된 장괴사의 소견이 발견되어 절제 및 문합술을 실시 하였다. 병리학적 검사에서는 장벽경색의 소견을 보였다. 수술후에 복통은 완화되었으나 경미하게 지속되었고, 제 13병일경에 급성 부고환염이 병발되었다가 회복되었고 제 15병일경에는 하지에서부터 발현된 자반이 3일간 지속되었다. 결구 Henoch-Schonlein 자반증과 이의 합병증으로 발생한 근위 공장의 장벽경색으로 진단하였다. Colicky abdominal pain as symptom of Henoch-Schonlein purpura occurs in up to 70% of patients, and results from submucosal edema and hemorrhage. Abdominal pain and other GI symptoms such as nausea, vomiting, melena and hematemesis resolve mostly without any complications. But rarely life-threatening GI complications such as intussusception, intestinal mural infarction and necrosis, and massive hemorrhage may develope. In 14% of patients, abdominal pain may precede other symptoms, and creats diagnostic difficulties. We reports a case of the Henoch-Schonlein purpura presented with colicky abdominal pain which resulted from intestinal mural infarction and preceded characteristic purpuric skin lesion by several weeks. This 5 year old male patient complained of colicky abdominal pain, nausea and melena. Exploratory laparatomy was done and revealed intestinal mural infarction in proximal jejunum. One week later operation, purpuric skin lesion develiped in both leg and feet, and then, Henoch-Schonlein purpura was diagnosed.

족삼리의 전기침 자극에 대한 개구반사 크기의 변화

이상호,나창수,송형근,서영아 大韓小兒齒科學會 1998 大韓小兒齒科學會誌 Vol.25 No.4

The purpose of this study was to investigate the effect of various electroacupuncture duration induced by acupuncture point-Zusanli(S36) electrical stimulation on inhibition of amplitude of digastric electromyogram (dEMG)evoked by noxious electrical stimuli around the mental foramen. <Experiments were conducted on adult rats (SD) were anesthetizde with> intraperitoneal sodium pentobarbital in an initial dose of 50mg/kg and maintenance doses of 4.5mg/kg/h were given through a cannula in the femoral vein using a constant infusion pump. A pair of stimulating electrodes were inserted for noxious stimuli around the mental foramen. An irritant electronic stimuli pulse (0.2 Hz,0.1 ms duration) was produced with an intensity of about 1.5-2 times threshold for evoking the dEMG. The anterior belly of the digastric muscle was exposed and a pair of 0.1mm wire electrodes were inserted for dEMG recording. Acupuncture point stimulation on Zusanli (2 Hz, 250㎲, biphasic pulse. 2 V) was delivered by Dental Electronic Anesthesia (3M,U.S.A). For periods of electronic stimulation of 10, 20, and 30min, the amplitudes of dEMG were measured on the oscilloscope and on the monitor connected to the amplifier. The following results were obtained: The dEMG was decreased to 73.4% of that in the control set after 10 min electroacupunture stimulation (Group Ⅰ): The dEMG was decreased to 77.1%(10min), 54.0% (20min)of that in the control set after 20minutes of electroacurunture stimulation (Group Ⅱ). The dEMG was decreased to 73.7% (10min), 61.9%(20min), 76.2%(30min) of that in the control set after 30 min of electroacupunture stimulation (Group Ⅲ). From these results, it may be that in the electroacupuncture stimulation on the Zusnali resulted in a reduction of amplitude of daEMG and that the most effective electroacupuncutre stimulation period was 20min.

장상피화생과 Helicobactor pylori와의 상관 관계에 관한 연구

이복희,김대수,이현숙,진영주,윤세진,송형근,성노현,정현용,이동호 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

In 1965 Lauren divided advanced gastric carcinoma into two main types-namely, "intestinal" and "diffuse" type carcinoma, which differ not only morphologically but also in their clinical and epidemiological characteristics. Moreover, a different histogenetic process has been postulated by many authors that intestinal type gastric carcinoma might arise from areas with intestinal metaplasia. Since Warren and Marshall reported the presence of Helicobacter pylori in the stomach in 1983, strong evidence has been obtained that there is a close relationship between Helicobacter pylori and chronic gastritis, atrophic gastritis, intestinal metaplasia, and intestinal type gastric carcinoma. To study the relationship between intestinal metaplasia and Helicobacter pylori infection, we performed gastroscopic biopsies and CLO tests taken from patients with epigastric pain. Only 87 patients with macroscopically suspected antral gastritis were included. The prevalence of intestinal metaplasia in gastric antral biopsies increased from 33.3% in the age group<30 years to 63.6% in the age group 60 years. When all patients were divided into two age groups-namely, (i)<50 years(n=41) and (ii)≥ 50 years(n-=46), intestinal metaplasia was found significantly more often in patients ≥ 50 years. ((i) 39.2%, (ii) 54.3%, p<0.0001). The prevalence of Helicobacter pylori in gastric antral biopsies decreased from 66.7% in the age group<30 years to 62.5%, in the age group in the age group 50 to 59 years. When all patients were divided into the same two age groups-namely(i)<50 years and (ii)≥50 years, the prevalence of Helicobacter pylori did not differ between two groups in a statistically significant way. The mean age of intestinal metaplasia positive patients who were also Helicobacter pylori positive was 48±11.0(mean±S.D.)years, whereas the mean age of intestinal metaplasia positive patients who were Helicobacter pylori negative was 52±9.7(mean±S.D.)years. In conclusion. although our data do not prove a causal relationship between Helicobacter pylori infection and the histogenesis of intestinal metaplasia, we suggest that Helicobacter pylori plays an important role in the development of intestinal metaplasia in the gastric mucosa. Whether Helicobacter pylori has to be present at all times during this process or is only necessary as a 'trigger', needs further research.