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RISS 인기검색어
- 검색키워드
- 저자 : Heeil Do (검색결과 6 건)
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Investigation of Fungal Strains Composition in Fruit Pollens for Artificial Pollination
( Heeil Do ),( Su-hyeon Kim ),( Gyeongjun Cho ),( Da-ran Kim ),( Youn-sig Kwak ) 한국균학회 2021 Mycobiology Vol.49 No.3
Plants pollination are conducted through various pollinators such as wind, animals, and insects. Recently, the necessity for artificial pollination is drawing attention as the proportion of natural pollinators involved is decreasing over the years. Likewise, the trade in pollen for artificial pollination is also increasing worldwide. Through these imported pollens, many unknown microorganisms can flow from foreign countries. Among them, spores of various fungi present in the particles of pollen can be dispersed throughout the orchard. Therefore, in this study, the composition of fungal communities in imported pollen was revealed, and potential ecological characteristics of the fungi were investigated in four types of imported pollen. Top 10 operational taxonomic unit (OTU) of fungi were ranked among the following groups: Alternaria sp., Cladosporium sp., and Didymella glomerata which belong to many pathogenic species. Through FUNGuild analysis, the proportion of OTUs, which is assumed to be potentially plant pathogens, was higher than 50%, except for apple pollen in 2018. Based on this study of fungal structure, this information can suggest the direction of the pollen quarantine process and contribute to fungal biology in pollen.
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Screening Antifungal and Exceptional Colonization Strains from Nationwide Actinobacteria Library
곽연식,Heeil Do,Da-Ran Kim 한국농약과학회 2022 농약과학회지 Vol.26 No.3
Since the Industrial Revolution, the population has grown exponentially and by 2050, the world's population is expected up to 9 billion. As the population increases, food supply is required to about 1.5 times the current amount, but the occurrence of diseases and environmental stress on crops due to climate change is increasing and crop production is decreasing. Chemical pesticides have contributed greatly to the increase in food production and security. However, due to issues of environmental, eco-friendly management techniques are demanded. There are many plants beneficial microorganisms that have been known to contribute to preventing plant disease occurrence as eco-friendly approaches in lab or greenhouse experimental scales. However, the candidate beneficial microbes failed to protect the plant against pathogens or abiotic stresses in commercial crop cultivated field conditions, due to a lack of colonization ability on plants. Therefore, this study aimed to secure outstanding colonization of microbial strains among Actinobacteria collection nationwide. Of the 2,114 isolates of Actinobacteria, two strains showed aggressive antifungal activity against a wide range of fungal pathogens and exceptional colonization ability in the plant rhizosphere. The strains have an excellent possibility to be biological control agents and members of the synthetic community for plant disease control.
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Comparison of Microbial Community of Rhizosphere and Endosphere in Kiwifruit
Kim, Min-Jung,Do, Heeil,Cho, Gyeongjun,Jeong, Rae-Dong,Kwak, Youn-Sig The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.6
Understanding the microbial community and function are crucial knowledge for crop management. In this study, bacterial and fungal community structures both rhizosphere and endosphere in kiwifruit were analyzed to gain our knowledge in kiwifruit microbiome. Microbial community in rhizosphere was less variation than endosphere community. Functional prediction results demonstrated that abundance of saprotrophic fungi was similar in both rhizosphere and endosphere, but potential pathogenic fungi was more abundance in endosphere than in rhizosphere. This finding suggested that maintain healthy soil is the first priority to protect the host plant against biotic stresses.
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Su-Hyeon Kim,Heeil Do,Gyeongjun Cho,Da-Ran Kim,Youn-Sig Kwak 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.3
Pollination is an essential process for plants to carry on their generation. Pollination is carried out in various ways depending on the type of plant species. Among them, pollination by insect pollinator accounts for the most common. However, these pollinators have be decreasing in population density due to environmen- tal factors. Therefore, use of artificial pollination is increasing. However, there is a lack of information on microorganisms present in the artificial pollens. We showed the composition of bacteria structure present in the artificial pollens of apple, kiwifruit, peach and pear, and contamination of high-risk pathogens was in- vestigated. Acidovorax spp., Pantoea spp., Erwinia spp., Pseudomonas spp., and Xanthomonas spp., which are classified as potential high-risk pathogens, have been identified in imported pollens. This study presented the pollen-associated bacterial community structure, and the results are expected to be foundation for strength- ening biosecurity in orchard industry.
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Pseudomonas capsici에 의한 아주까리 세균점무늬병의 국내 첫 보고
도희일,이승엽,이방울,함현희,이미현,이영기,Heeil Do,Seung Yeup Lee,Bang Wool Lee,Hyeonheui Ham,Mi-Hyun Lee,Young Kee Lee 한국식물병리학회 2023 식물병연구 Vol.29 No.4
2021년 8월 부산광역시 강서구에서 아주까리의 잎과 줄기에 점무늬병의 발생이 확인되었다. 아주까리 분리균은 tryptic soy agar에서 점액성이 없고 연한 녹색을 띠는 둥근 콜로니를 형성하였다. 세균은 단극성으로 다수의 편모를 가진 막대 모양이었다. 세균을 건전한 아주까리 유묘에 접종했을 때, 동일한 점무늬 증상의 괴사 반응이 나타났다. 병징이 나타난 부위에서 세균을 재분리하여 동일균 여부를 확인하였다. 분리균은 감자 무름 반응과 D-fucose 대사반응이 양성이었으며, troleandomycin에서 생장할 수 있었다. 16S rRNA와 항존유전자(gyrB, rpoD) 염기서열의 상동성을 비교하였을때, Pseudomonas capsici와 유연관계가 가장 높았다. 결과적으로 아주까리에서 분리된 균은 배양적, 생화학적, 유전적 특성에 의해 P. capsici로 동정되었다. 본 연구는 국내 아주까리에서 P. capsici에 의한 점무늬병의 최초 보고이다. In August 2021, water-soaking symptoms of bacterial spot disease were observed on castor bean in a field in Gangseo District, Busan. Bacteria isolated from the lesion when cultured on tryptic soy agar appeared to be nonmucoid and pale green. To confirm whether the isolates were the causative agent of the spot disease, they were inoculated onto healthy castor bean plants. The same symptoms were observed on the inoculated tissue, and the bacteria were reisolated from the lesion. Furthermore, the isolates were consistent with the biochemical and physiological features of Pseudomonas capsici. Sequencing analysis using 16S rRNA and housekeeping genes (gyrB, rpoD) showed that the isolates shared a high sequence similarity with P. capsici. These results confirmed that the strains belonged to P. capsici. To our knowledge, this is the first report of bacterial spot disease caused by P. capsici on castor bean in Korea.
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