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서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)
김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1
In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.
한승규(Seung-Kyoo Han),전평(Pyoung Jeon) 대한소아신경학회 1999 대한소아신경학회지 Vol.7 No.1
본 저자는 출생 후 정상 발달을 보였고 경련의 과거력이 없었던 3세 환아에서, 임상적으로 약 24시간 동안 의식의 혼탁을 보여 시행한 뇌파 소견상 국소적인 극서파가 지속적으로 관찰되어 복합부분 발작 중첩증으로 진단한 증례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Nonconvulsive status epilepticus, which constitutes about 25% of all cases of status, has been subdivided into generalized nonconvulsine(absence) status and complex partial status. We report a case of complex partial status epilepticus in a 3-year-old male patient with altered mental function lasting about 24 hours. The electroencephalographic finding was compatible with complex partial status and the outcome of treatment is excellent, so far.
Duchenne형 근이양증에서 PCR을 응용한 Dystrophin 유전자 변이 분석
한승규(Seung-Kyoo Han),김종욱(Jong-Wook Kim),손병관(Byong-Kwan Son),채종희(Jong-Hee Chae),황용승(Yong-Seung Hwang) 대한소아신경학회 2000 대한소아신경학회지 Vol.8 No.2
목적 : Duchenne형 근인양증에서 나타나는 돌연변이는 유전자 결실 65%, 중복 5%, 그리고 점돌연변이, 다형성, 뉴클레오타이드 소변이 등의 미세병변 30%로 알려져 있다. 유전자 결실은 multiplex PCR이나 Southern blot을 이용하여 검출할 수 있으나, 미세병변은 검출을 위한 여러 연구가 시도되고 있지만 아직 뚜렷한 정론이 내려져 있지 않다. 이에 저자들은 환자의 말초 혈액을 채취하여 게놈 DNA를 분리한 후, PCR과 관련된 분자 진단법을 이용하여 유전자 변이 분석을 하였다. 방법 : 서울대학교 어린이병원 소아과에서 근육 조직 검사에 의하여 Duchenne형 근이양증으로 확진된 환자 중 외래 추적 관찰이 가능한 환자 5례와 그 환자의 모친 혹은 자매 7례 즉 총 12례를 대상으로 하였다. 이들로 부터 말초 혈액을 채취하여 게놈 DNA를 분리하였다. 총 28쌍의 시발체를 이용하여 multiplex PCR을 시행하였다. Exon 중복을 분석하기 위하여 multiplex PCR과 겔밀도측정법을 시행하여 부모와 다음 세대간의 띠강도를 비교하였다. 결실이 발견되지 않은 군은exon 각각에 대한 SSCP 분석과 직접 염기 서열분석을 하였다. 결과 : 혈액 1mL로 부터 게놈 DNA 12-2O㎍을 추출할 수 있었으며 크기는 5kb 이상이었다. 게놈 DNA를 이용하여 muitiplex PCR을 수행하였고 최고 9개의 PCR 산물을 얻을 수 있었다. 유전자 변이는 5례의 환자 중 4례에서 관찰되었다. 결실은 2례에서 관찰되었으며, 위치는 각각 exon 45, 47-53, 그리고 exon 47-49, 53이었다. 중복은 1례에서 관찰되었으며, exon 43에 위치하고 있었다. 결실이나 중복이 관찰되지 않았던 1례에서 미세병변이 관찰되었으며, exon 43의 A 염기 결실과 Intron 49의 C→G transversion 변이가 관찰되었다. 결론 : 저자들은 침습적 기법을 필요로 하는 근육 조직이 아닌 가장 손쉽게 얻을 수 있는 말초 혈액을 채취한 후, PCR, SSCP, 겔 밀도측정법, 직접 염기 서열 분석법 등의 방법을 체계적으로 이용하여 Duchenne형 근이양증에서 나타나는 여러 종류의 유전자 변이를 분석할 수 있었으며, 좀더 많은 증례를 대상으로 한 연구가 필요하리라 사료된다. Purpose: Large rearrangements in the dystrophin gene is detected in about 65-70% of patients by multiplex PCR or Southern blot, although detection of point mutations and microlesions is currently in progress. The purpose of this study is to carry out mutation analysis of the dystrophin gene by application of PCR-related molecular diagnostic methods in Duchenne muscular dystrophy(DMD). Methods: Five patients diagnosed as a DMD by muscle biopsy, and their first-degree relatives and mothers were enrolled in this study. The genomic DNA was extracted from the peripheral blood lymphocyte. We used a total of 28 pairs of primers including Beggs´ and Chamberlain´s primers. The multiplex PCR was performed in 4 groups; 5´Rxn2, 3´Rxn2, Rxn2, and CRxn2. For exon duplication analysis, multiplex PCR and gel densitometry were carried out by comparing the band intensities among individual bands. For groups with no detectable deletion, single strand conformation polymorphism(SSCP) analysis and direct DNA sequencing method were performed with individual PCR of candidate exons. Results: About 12-20μg of genomic DNA was extracted from 1mL of blood, and the size of DNA was over 50kb. Up to 9 PCR products were made from multiplex PCR using the genomic DNA. Among 5 families with DMD, No. 6 had about 240kb DNA deletion from exon 45 and 47-53, and No. 11 had about 130kb deletion from exon 47-49 and 53. No. 1 showed duplication of exon 43 when the multiplex PCR products were analyzed by a densitometer. When the deletion/duplication negative No. 3 was analyzed by SSCP method, exon 43 and 49 showed abnormal band patterns. The abnormal band pattern of exon 43 was caused by deletion mutation of A residue, which resulted in pretermination of dystrophin synthesis, meanwhile exon 49 showed transversion mutation of C→G at intron 49. Conclusion: Based on the results of this study, the methods of multiplex PCR, SSCP and direct sequencing of PCR products made it possible to analyze several types of mutation of DMD.
다공질 Calcium Polyphosphate의 생분해성에 관한 연구
양승민,이영규,한은영,김석영,계승법,이승진,이용무,구영,한수부,정종평,최상묵,류인철,Yang, Seung-Min,Lee, Young-Kyoo,Han, Eun-Young,Kim, Seok-Young,Kye, Seung-Beom,Lee, Seung-Jin,Lee, Yong-Moo,Ku, Young,Han, Soo-Boo,Chung, Chong-Pyoung,Ch 대한치주과학회 2001 Journal of Periodontal & Implant Science Vol.31 No.3
The purpose of this study is to evaluate the bioresorbability of Calcium Polyphosphate added with $Na_2O$ and chitosan. Though calcium phosphate ceramics meet some of the needs for bone replacement, they have some limitation of unresorbability and fibrous encapsulation without direct bone apposition during bone remodelling. To solve these problem, we developed a new ceramic, calcium polyphosphate(CPP), and report the biologic response to CPP in extraction sites of beagle dog. Porous CPP granules were prepared by condensation of anhydrous $Ca(H_2PO_4)_2$ to form non-crystalline $Ca(PO_3)_2$. CPP granules added with $Na_2O$ and chitosan were implanted in extraction sockets and histologic observation were performed at 12 weeks later. Histologic observation at 12 weeks revealed that CPP matrix were mingled with and directly apposed to new bone without any intervention of fibrous connective tissue. CPP granules added with chitosan were well adatped without any adverse tissue reaction and resorbed slowly and spontaneously. CPP granules added with $Na_2O$ and chitosan show multinucleated giant cells and osteoblast-like cells around grafted material and newly formed bone. This result revealed that CPP, regardless of its additive component, had a high affinity for bone and had been resorbed slowly. From this results, it was suggested that CPP is promising ceramic as a bone substitute and addition of $Na_2O$ and chitosan help biodegradation. In further study , it will be determined which concentration of $Na_2O$ help biodegradation and the other additive components increase the degradation rate.
만성 성인T세포백혈병/림프종 환자에서 병발한 거대세포바이러스 장염 1예
박한승,김대영,김지범,김윤구,조민수,옥태진,장선주,이규형 영남대학교 의과대학 2011 Yeungnam University Journal of Medicine Vol.28 No.2
Adult T-cell leukemia/lymphoma (ATLL) is a malignancy of mature T-cells caused by the human T-cell lymphotrophic virus type I (HTLV-I). HTLV-I is endemic in some areas in Japan, the Caribbean basin, and Africa but has low prevalence in South Korea. Patients with ATLL are susceptible to opportunistic infections such as cytomegalovirus (CMV) infection, but CMV infection in chronic ATLL is uncommon. Reported herein is a case involving a 44-year-old woman with chronic ATLL who presented the symptoms of fever and diarrhea. She was suspected to have acute-type ATLL but was later diagnosed with CMV colitis.