An Early-Maturing, High-quality and High-yielding Rice Variety "Oraebyeo"

Jin Kwan Ham,Young Boum Shin,Youn Sang Cho,Jae Rok Kim,Soon Kwan Hong,Yong Bok Kim,An Soo Lee,Jong Goo Sa,Seong Kyung Kim,Jeong Ki Hong 한국육종학회 2007 한국육종학회지 Vol.39 No.4

"Oraebyeo" is mutation of a high quality japonica cultivar, Ilpumbyeoa new japonica rice(Oriza sativa L.) cultivar developed from by N-methyl-N-nitrosourea(MNU) treatment on fertilized egg cell by the rice breeding team of Gangwon Provincial Agricultural Res. & Ext. Service in 2006. This variety heads 20 days earlier than Ilpumbyeo and similar to that of Odaebyeo an early maturing leading cultivar of northern plain area of Korea. Compared with Odaebyeo has a good semi-erect plant type with lodging tolerance due to much shortened plant hight(l2cm shorter than Odaebyeo). Oraebyeo has similar panicle number with Odaebye where as it has more spikelets per panicle. However, this variety less the ratio of ripened grains and 1,000 grain weight than those of Odaebyeo. The yield performance of this cultivar in milled rice was about 5.09MT/ha in local adaptability test conducted for three years 2002-2004 and 5.51MT/ha in the demonstration plot of farmer's field.

잔대의 함유성분 분석과 항산화 활성

함영안(Young-An Ham),최현진(Hyun-Jin Choi),정미자(Mi-Ja Chung),함승시(Seung-Shi Ham) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.3

잔대 뿌리의 무기질, 아미노산, 지방산, 당류 함량과 항산화 효과에 대해서 조사하였다. 수분, 조단백 조지방, 조회분과 탄수화물은 각각 3.4±0.3, 9.4±0.1, 1.6±0.1, 3.7±0.2와 81.9±0.5%이었다. 무기질 중에 칼륨의 함량이 가장 높게 나타났으며, 총 아미노산 함량은 103.5±9.1 ㎎/g이었고, 지방산에서 palmitic acid가 가장 많았다. 잔대 뿌리에 함유된 비타민 E와 C는 각각 1.1±0.1과 5.2±0.5 ㎎/100 g이었다. 잔대 70% 에탄올, 헥산, 클로로포름, 에틸아세테이트, 부탄올과 물 추출물에서 DPPH 라디칼 소거능의 항산화 효과 결과는 RC50값이 265.4, 117.1, 103.7, 104.4, 108.9, 484.1 ㎍로 나타났다. The levels of minerals, amino acids, fatty acids and sugars and antioxidant capacity of Adenophora triphylla (AT) root were investigated. The moisture, crude protein, crude lipid, crude ash and carbohydrate contents of the AT root were 3.4±0.3, 9.4±0.1, 1.6±0.1, 3.7±0.2, and 81.9±0.5, respectively. Among the minerals present in root, potassium content was the highest. Total amino acid contents were 103.5±9.1 ㎎/g. Palmitic acid was the most abundant fatty acid in AT root. The vitamin E and C levels in AT root were 1.1±0.1 and 5.2±0.5 ㎎/100 g, respectively. The antioxidant activity of 70% ethanol extract and fractions of the AT root has been determined by the scavenging of the stable radical DPPH; the result showed that the chloroform fraction was the most active, as the amount required for 50% reduction of DPPH after 30 min (RC50) was 103.7 ㎍, followed by 70% ethanol extract (265.4 ㎍), hexane fraction (104.4 ㎍), ethyl acetate fraction (104.4 ㎍), butanol fraction (108.9 ㎍) and aqueous fraction (484.1 ㎍).

잔대 추출물들의 항돌연변이 및 항종양 효과

함영안(Young-An Ham),최현진(Hyun-Jin Choi),김수현(Soo-Hyun Kim),정미자(Mi-Ja Chung),함승시(Seung-Shi Ham) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.1

본 연구는 잔대의 돌연변이원성, 항돌연변이원성, 세포독성, 항종양 효과를 조사하기 위해서 수행되었다. 잔대를 70% 에탄올로 추출하여 추출용매에 따라 핵산, 클로로포름, 에틸아세테이트, 부탄올과 물층으로 분획하였다. Ames test, SRB assay와 종양 억제실험 방법을 사용하여 실험하였다. Ames test결과, 잔대 에탄올 추출물과 그 분획물들은 돌연변이원성을 나타내지 않았을 뿐만 아니라, MNNG와 4NQO로 돌연변이를 일으켰을 때 높은 항돌연변이 효과를 나타내었다. 잔대 에틸아세테이트 분획물은 S. Typhimurium TA98를 4NQO로 돌연변이를 유도하였을 때 66.5%의 억제율을 나타내었으며, S. Typhimurium TA100에서 MNNG와 4NQO로 돌연변이를 유도했을 때는 83.3%와 75.1%의 억제율을 나타내었다. 잔대 추출물 및 그 분획물들의 암세포 성장 억제효과를 살펴보기 위해 인간 자궁암세포(HeLa), 인간 간암세포(Hep3B), 인간 유방암세포(MCF-7), 인간 위암세포(AGS), 인간 폐암세포(A549)와 인간 신장정상세포(293)를 사용하였다. 잔대 에틸아세테이트 분획물을 1 ㎎/mL를 처리하였을 때 각각 79.9%(HeLa), 74.9%(Hep3B), 66.0%(MCF-7), 71.0%(AGS)와 74.3%(A549)의 가장 높은 억제활성을 나타내었다. 반면에 인간 정상 신장세포에서는 3~36%의 세포독성을 나타내었다. In vivo에서 잔대 추출물의 항암효과를 시험하기 위하여 Balb/c 마우스에 sarcoma-180 종양세포로 고형암을 유발시켰다. 그 결과 잔대 에틸아세테이트 층의 최고농도 50 ㎎/㎏에서 37.2%의 고형암 성장 억제효과를 나타내었고, 이는 모든 처리군 중 가장 높은 억제율이었다. This study was carried out to investigate the mutagenic, antimutagenic, cytotoxicity and antitumor effects of Adenophora triphylla (AT). AT was extracted with 70% ethanol and then further fractionated to hexane, chloroform, ethyl acetate, butanol and water. Antimutagenic, cytotoxicity and antitumor effects of AT extracts were measured by using Ames test, SRB method, and the tumor growth inhibition test. AT extracts did not show any mutagenicity in the Ames test; however, 70% ethanol extracts and its fractions had strong antimutagenic effects against mutation induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and 4-nitroquinoline-1-oxide (4NQO). The ethyl acetate fraction of AT (200 ㎍/plate) showed approximately 66.5% inhibitory effect on the mutagenesis induced by 4NQO against TA98 strain, whereas 83.3% and 75.1% inhibitions were observed on the mutagenesis induced by MNNG and 4NQO against TA100 strain. In anticancer effects, the cytotoxicity of AT extract and its fractions against cancer cell lines including human cervical adenocarcinoma (HeLa), human hepatocellular carcinoma (Hep3B), human breast adenocarcinoma (MCF-7), human gastric carcinoma (AGS), human lung carcinoma (A549) and transformed primary human embryo kidney (293) were investigated. The treatment of 1 ㎎/mL AT ethyl acetate faction had the highest cytotoxicity of 79.9%, 74.9%, 66.0%, 71.0% and 74.3% against HeLa, Hep3B, MCF-7, AGS and A549 cells, respectively. In contrast, the extract and its fractions showed only 3~36% cytotoxicity for a normal human kidney cell line (293). In vivo anti-cancer effect of Adenophora triphylla extract was tested using Balb/c mice transplanted sarcoma-180 cells. Adenophora triphylla ethyl acetate fraction showed the highest inhibition rate of 37.2% at the 50 ㎎/㎏ concentration.

발관 실패의 위험 인자 및 발관 후 천음과 재삽관의 예측에 있어 Cuff Leak Test의 유용성과 의미 분석

임성용 ( Seong Yong Lim ),서지영 ( Gee Young Suh ),경선영 ( Sun Yong Kyung ),안창혁 ( Chang Hyeok An ),박정웅 ( Jung Woong Park ),이상표 ( Sang Pyo Lee ),정성환 ( Sung Hwan Jeong ),함형석 ( Hyoung Suk Ham ),안영미 ( Young Mee A 대한결핵 및 호흡기학회 2006 Tuberculosis and Respiratory Diseases Vol.61 No.1

연구배경: 발관 실패와 관련된 위험 인자를 살펴보고, 기관내 삽관의 합병증으로 인한 후두부 폐쇄와 PES로 인한 재삽관의 위험성을 발관 전에 미리 예측하기 위한 CLT 검사의 의의 및 제한점을 알아보고자 하였다. 방법: 24시간 이상 삽관과 인공 호흡기 치료 후 발관을 시도한 34명의 연속적인 환자들을 대상으로 발관 실패군에서 성공군과 차이를 보이는 인자를 살펴보고 CLT를 시행해서 PES와 재삽관을 예측하는 CLV, CLP의 기준값을 구하였다. 결과: 총 34명중 발관 실패는 6명(17.6%), PES는 3명(8.8%) 이었고 PES가 있던 3명은 모두 재삽관을 받았다. 발관 실패 환자에서 삽관 기간이 길었고, SAPS II score가 컸으며, 삽관 기간은 CLV, CLP와 역상관관계를 보였다. PES 양성 환자의 평균 CLV은 22.5±23.8㎖로 음성 환자의 233.3±147.1㎖보다 낮았고, CLP도 양성군에서 6.2±7.3%로 음성군의 44.3±24.7%보다 유의하게 낮았다. 가장 신뢰성 있는 CLV, CLP의 기준값은 50㎖, 14.7%였고 CLV의 민감도는 100%, 특이도 93.5%, 양성예측도 60%, 음성예측도 100%였고, CLP의 민감도는 100%, 특이도 87.1%, 양성예측도 42.9%, 음성예측도 100%였다. 결론: 삽관 기간이 길고 중증 질환 환자에서 발관 실패의 가능성이 높다. CLV, CLP가 감소되어 있을 경우 PES로 인한 재삽관 위험이 높아지므로 발관 시 주의가 필요하지만 발관의 불필요한 연기나 시도 자체에 대한 기준은 아닐 것으로 생각된다. Background: Extubation failure was associated with poor prognosis and high hospital mortality. Cuff leak test (CLT) has been proposed as a relatively simple method for detecting laryngeal obstruction that predispose toward postextubation stridor (PES) and reintubation. We examined the risk factors of extubation failure and evaluated the usefulness and limitation of CLT for predicting PES and reintubation. Methods: Thirty-four consecutive patients intubated more than 24 hours were examined. The subjects were evaluated daily for extubation readiness, and CLT was performed prior to extubation. Several parameters in the extubation success and failure group were compared. The accuracy and limitation of CLT were evaluated after choosing the thresholds values of the cuff leak volume (CLV) and percentage (CLP). Results: Of the 34 patients studied, 6 (17.6%) developed extubation failure and 3 (8.8%) were accompanied by PES. The patients who had extubation failure were more likely to have a longer duration of intubation and more severe illness. The patients who developed PES had a smaller cuff leak than the others: according to the CLV (22.5±23.8 vs 233.3±147.1㎖, p=0.020) or CLP (6.2±7.3 vs 44.3±24.7%, p=0.013). The best cut off values for the CLV and CLP were 50㎖ and 14.7%, respectively. The sensitivity, negative predictive value, and specificity of CLT were relatively high, but the positive predictive value was low. Conclusion: The likelihood of developing extubation failure increases with increasing severity of illness and duration of intubation. A low CLV or CLP (<50㎖ or 14.7%) is useful in identifying patients at risk of PES, but the CLT is not an absolute predictor and should not be used an indicator for delaying extubation. (Tuberc Respir Dis 2006; 61: 34-40)

Upregulation of miR-23b Enhances the Autologous Therapeutic Potential for Degenerative Arthritis by Targeting PRKACB in Synovial Fluid-Derived Mesenchymal Stem Cells from Patients

Ham, Onju,Lee, Chang Youn,Song, Byeong-Wook,Lee, Se-Yeon,Kim, Ran,Park, Jun-Hee,Lee, Jiyun,Seo, Hyang-Hee,Lee, Chae Yoon,Chung, Yong-An,Maeng, Lee-So,Lee, Min Young,Kim, Jongmin,Hwang, Jihwan,Woo, Don Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.6

The use of synovial fluid-derived mesenchymal stem cells (SFMSCs) obtained from patients with degenerative arthropathy may serve as an alternative therapeutic strategy in osteoarthritis (OA) and rheumatoid arthritis (RA). For treatment of OA and RA patients, autologous transplantation of differentiated MSCs has several beneficial effects for cartilage regeneration including immunomodulatory activity. In this study, we induced chondrogenic differentiation of SFMSCs by inhibiting protein kinase A (PKA) with a small molecule and microRNA (miRNA). Chondrogenic differentiation was confirmed by PCR and immunocytochemistry using probes specific for aggrecan, the major cartilaginous proteoglycan gene. Absorbance of alcian blue stain to detect chondrogenic differentiation was increased in H-89 and/or miRNA-23b-transfected cells. Furthermore, expression of matrix metalloproteinase (MMP)-9 and MMP-2 was decreased in treated1 cells. Therefore, differentiation of SFMSCs into chondrocytes through inhibition of PKA signaling may be a therapeutic option for OA or RA patients.

Comparison of Quality Characteristics of Hot Water Extract of Maitake (Grifola frondosa) Mushrooms according to Outdoor Cultivation and Bag Cultivation

An Soo Lee(이안수),Ji Seon Park(박지선),Jin Kwan Ham(함진관),Eun Ha Chang(장은하),Kyung Dae Kim(김경대),Jae Geel Lim(임재길),Jeung Ae Yoon(윤정애),Han Ul Park(박한울),Kyung Ah Jang(장경아),Sun Young Kim(김선영),Kye Hyun Lim(임계현) 한국약용작물학회 2021 한국약용작물학회 학술대회논문집 Vol.2021 No.1

바이오 분야 적용을 위한 마이크로 젯 인젝션 실험

함영복(Young-Bog Ham),안병철(Byeung-Cheol An),Mojiz Abbas Trimzi,김종대(Jong-Dae Kim),이기태(Gi-Tae Lee),박중호(Jung-Ho park),윤소남(So-Nam Yun) 대한기계학회 2016 大韓機械學會論文集B Vol.40 No.10

마이크로 인젝션을 바이오분야에 적용하기 위해 고압생성을 통해 침투하는 방법을 채택하였다. 그러나 고압의 액체를 인젝션 할 경우 그 양을 미세하게 하기 어렵다. 이러한 문제를 해결하기 위해는 고속으로 동작하는 개폐밸브에 의한 방법이 있다. 본 연구에서는 수백 Hz로 동작가능한 압전 액추에이터로 구성된 개폐밸브를 적용하여 실험하였다. 압전밸브를 구동하는 파형을 다양하게 조절하고 노즐의 구조에 의한 영향을 알아보기 위해 5가지 서로 다른 치수의 노즐을 제작하였다. 다양한 노즐과 구동파형 변수를 조절하면서 무엇이 인젝션 볼륨과 인젝션 힘에 큰 영향을 미치는지 확인하였다. 이 실험을 통해, 인젝션 볼륨을 줄이고 인젝션 힘을 증가시키는 방법을 알아냈고 목표치가 있다면 그에 맞는 다양한 값을 결정할 수 있게 되었다. It is essential for micro jet injectors in the biomedical sector to operate under high pressure. High pressure injection, however, is accompanied by high volumes. On/Off valves that can be operated at high speeds have been used to address this problem. In this research, piezoelectric actuators which have a response frequency of the order of hundreds of kilohertz were used as the On/Off valve and experiments were applied. Researchers developed a controller to precisely manipulate the piezoelectric valve with various waveforms. They also fabricated five types of nozzles to consider the effect of nozzle type on injection. This allowed researchers to manipulate and confirm factors that can affect the injection volume and force. Results of this experiment have shown how to decrease the injection volume and increase the injection force. and it is predicted that the optimized injection volume and force value can be determined depending on the skin type.

사판식 액셜피스톤 수압 펌프 개발과 응용

함영복(Young Bog Ham),안병철(Byeung Cheol An) 유공압건설기계학회 2016 드라이브·컨트롤 Vol.13 No.3

전문 자격별 간호장교의 직무 만족도 조사

함영희 ( Young Hee Ham ),안창숙 ( Chang Sook An ),김은희 ( Eun Hee Kim ) 국군간호사관학교 군진간호연구소 1989 군진간호연구 Vol.9 No.-

한국기계연구원의 유공압건설기계 관련 정부과제 수행 현황 소개

함영복(Young-Bog Ham),김홍욱(Hong-Uk Kim),안병철(Byeung-Cheol An) 유공압건설기계학회 2020 드라이브·컨트롤 Vol.17 No.4